BR9909459A - Protocolo de transformação de soja de eficiência melhorada - Google Patents
Protocolo de transformação de soja de eficiência melhoradaInfo
- Publication number
- BR9909459A BR9909459A BR9909459-2A BR9909459A BR9909459A BR 9909459 A BR9909459 A BR 9909459A BR 9909459 A BR9909459 A BR 9909459A BR 9909459 A BR9909459 A BR 9909459A
- Authority
- BR
- Brazil
- Prior art keywords
- transformation
- protocol
- soybean
- improved efficiency
- processing protocol
- Prior art date
Links
- 235000010469 Glycine max Nutrition 0.000 title abstract 6
- 244000068988 Glycine max Species 0.000 title abstract 5
- 230000009466 transformation Effects 0.000 abstract 5
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000005562 Glyphosate Substances 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 abstract 1
- 229940097068 glyphosate Drugs 0.000 abstract 1
- 210000001161 mammalian embryo Anatomy 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
Patente de Invenção: <B>"PROTOCOLO DE TRANSFORMAçãO DE SOJA DE EFICIêNCIA MELHORADA"<D>. Um protocolo de transformação é descrito para a transformação mediada por partículas de soja. Este protocolo é com base na liberação de gene meristema de desenvolvimento de um embrião de soja. Antes da transformação dos protocolos com base no gene do caule médio ("meristema") a liberação em soja não dependeu da seleção devido à dificuldades em empregar agentes de seleção em tecido diferenciado em soja. Constatamos que uma cultura pós-bombardeio com a seleção de glifosato pode dramaticamente aumentar a eficiência de um tal protocolo de transformação.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/056,073 US5914451A (en) | 1998-04-06 | 1998-04-06 | Efficiency soybean transformation protocol |
PCT/US1999/006672 WO1999051759A1 (en) | 1998-04-06 | 1999-03-26 | Particle mediated transformation of soybean with glyphosate selection |
Publications (2)
Publication Number | Publication Date |
---|---|
BR9909459A true BR9909459A (pt) | 2000-12-12 |
BR9909459B1 BR9909459B1 (pt) | 2011-05-31 |
Family
ID=22001985
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI9909459-2A BR9909459B1 (pt) | 1998-04-06 | 1999-03-26 | processo eficaz de obtenção de plantas de soja de linhagem germinal transformadas usando seleção de glifosfato. |
Country Status (14)
Country | Link |
---|---|
US (1) | US5914451A (pt) |
EP (2) | EP1068341B1 (pt) |
JP (1) | JP2002510499A (pt) |
AT (2) | ATE328097T1 (pt) |
AU (1) | AU3209599A (pt) |
BR (1) | BR9909459B1 (pt) |
CA (1) | CA2325331A1 (pt) |
CY (1) | CY1104999T1 (pt) |
DE (2) | DE69931619T2 (pt) |
DK (1) | DK1068341T3 (pt) |
ES (1) | ES2265684T3 (pt) |
PT (1) | PT1068341E (pt) |
WO (1) | WO1999051759A1 (pt) |
ZA (1) | ZA200005411B (pt) |
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US5416011A (en) * | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
JPH03187381A (ja) * | 1989-11-17 | 1991-08-15 | Agracetus Inc | グルタミンシンテターゼインヒビター耐性に関するダイズ植物の遺伝子操作方法 |
CA2036935A1 (en) * | 1990-02-26 | 1991-08-27 | Paul Christou | Plant transformation process with early identification of germ line transformation events |
WO1992000377A1 (en) * | 1990-06-25 | 1992-01-09 | Monsanto Company | Glyphosate tolerant plants |
DK0546090T4 (da) * | 1990-08-31 | 2006-11-20 | Monsanto Co | Glyphosattolerante enolpyruvylshikimat-3-phosphat-synthaser |
US5633435A (en) * | 1990-08-31 | 1997-05-27 | Monsanto Company | Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases |
US5631152A (en) * | 1994-10-26 | 1997-05-20 | Monsanto Company | Rapid and efficient regeneration of transgenic plants |
WO1999018223A1 (en) * | 1997-10-07 | 1999-04-15 | EMBRAPA-Empresa Brasileira De Pesquisa Agropecuária | A process for obtaining transgenic leguminous plants (leguminosae) containing exogenous dna |
-
1998
- 1998-04-06 US US09/056,073 patent/US5914451A/en not_active Expired - Lifetime
-
1999
- 1999-03-26 CA CA002325331A patent/CA2325331A1/en not_active Abandoned
- 1999-03-26 DE DE69931619T patent/DE69931619T2/de not_active Expired - Lifetime
- 1999-03-26 EP EP99914194A patent/EP1068341B1/en not_active Expired - Lifetime
- 1999-03-26 PT PT99914194T patent/PT1068341E/pt unknown
- 1999-03-26 WO PCT/US1999/006672 patent/WO1999051759A1/en active IP Right Grant
- 1999-03-26 JP JP2000542470A patent/JP2002510499A/ja not_active Withdrawn
- 1999-03-26 AT AT99914194T patent/ATE328097T1/de active
- 1999-03-26 DK DK99914194T patent/DK1068341T3/da active
- 1999-03-26 DE DE69941439T patent/DE69941439D1/de not_active Expired - Lifetime
- 1999-03-26 AT AT06114739T patent/ATE443150T1/de not_active IP Right Cessation
- 1999-03-26 BR BRPI9909459-2A patent/BR9909459B1/pt not_active IP Right Cessation
- 1999-03-26 EP EP06114739A patent/EP1728871B1/en not_active Expired - Lifetime
- 1999-03-26 ES ES99914194T patent/ES2265684T3/es not_active Expired - Lifetime
- 1999-03-26 AU AU32095/99A patent/AU3209599A/en not_active Abandoned
-
2000
- 2000-10-04 ZA ZA200005411A patent/ZA200005411B/xx unknown
-
2006
- 2006-06-29 CY CY20061100887T patent/CY1104999T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
DE69931619T2 (de) | 2007-05-10 |
EP1728871B1 (en) | 2009-09-16 |
ES2265684T3 (es) | 2007-02-16 |
ZA200005411B (en) | 2002-03-27 |
EP1068341A1 (en) | 2001-01-17 |
US5914451A (en) | 1999-06-22 |
AU3209599A (en) | 1999-10-25 |
JP2002510499A (ja) | 2002-04-09 |
ATE328097T1 (de) | 2006-06-15 |
WO1999051759A1 (en) | 1999-10-14 |
CY1104999T1 (el) | 2009-11-04 |
ATE443150T1 (de) | 2009-10-15 |
EP1728871A2 (en) | 2006-12-06 |
EP1728871A3 (en) | 2006-12-13 |
CA2325331A1 (en) | 1999-10-14 |
DK1068341T3 (da) | 2006-07-10 |
DE69941439D1 (de) | 2009-10-29 |
PT1068341E (pt) | 2006-08-31 |
BR9909459B1 (pt) | 2011-05-31 |
DE69931619D1 (de) | 2006-07-06 |
EP1068341B1 (en) | 2006-05-31 |
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