BR9813099A - Seleção positiva-negativa na recombinação homóloga - Google Patents
Seleção positiva-negativa na recombinação homólogaInfo
- Publication number
- BR9813099A BR9813099A BR9813099-4A BR9813099A BR9813099A BR 9813099 A BR9813099 A BR 9813099A BR 9813099 A BR9813099 A BR 9813099A BR 9813099 A BR9813099 A BR 9813099A
- Authority
- BR
- Brazil
- Prior art keywords
- homologous recombination
- positive
- negative selection
- recombination
- genome
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Patente de Invenção: <B>"SELEçãO POSITIVA-NEGATIVA NA RECOMBINAçãO HOMóLOGA''<D>. A invenção refere-se a um processo para a introdução de um DNA estranho no genoma de uma célula visada mediante recombinação homóloga, bem como para a recombinação homóloga de construções de DNA adequadas.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97118175.5 | 1997-10-20 | ||
EP97118175 | 1997-10-20 | ||
PCT/EP1998/006616 WO1999020780A1 (de) | 1997-10-20 | 1998-10-20 | Positiv-negativ-selektion bei der homologen rekombination |
Publications (2)
Publication Number | Publication Date |
---|---|
BR9813099A true BR9813099A (pt) | 2000-08-22 |
BR9813099B1 BR9813099B1 (pt) | 2010-02-09 |
Family
ID=8227499
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI9813099-4A BR9813099B1 (pt) | 1997-10-20 | 1998-10-20 | processo para a introduÇço de dna exàgeno em uma cÉlula hospedeira mediante recombinaÇço homàloga, vetor recombinante, bem como emprego do referido vetor e de receptores superficiais em posiÇço de membrana. |
Country Status (17)
Country | Link |
---|---|
US (1) | US6284541B1 (pt) |
EP (1) | EP1025253B2 (pt) |
JP (1) | JP4417549B2 (pt) |
KR (1) | KR100566702B1 (pt) |
CN (1) | CN1146666C (pt) |
AR (1) | AR017368A1 (pt) |
AT (1) | ATE260982T1 (pt) |
AU (1) | AU730466B2 (pt) |
BR (1) | BR9813099B1 (pt) |
CA (1) | CA2306229C (pt) |
DE (1) | DE59810925D1 (pt) |
DK (1) | DK1025253T4 (pt) |
ES (1) | ES2213925T5 (pt) |
PT (1) | PT1025253E (pt) |
TR (1) | TR200001046T2 (pt) |
WO (1) | WO1999020780A1 (pt) |
ZA (1) | ZA989497B (pt) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7220221B2 (en) * | 2000-05-03 | 2007-05-22 | Nautilus, Inc. | Exercise device with body extension mechanism |
US6929909B2 (en) | 2000-09-15 | 2005-08-16 | Deltagen, Inc. | Gene targeting vectors comprising conditional positive selection markers |
WO2002055687A2 (en) * | 2000-12-13 | 2002-07-18 | Deltagen Inc | Transgenic mice containing target gene disruptions |
WO2003027261A2 (en) * | 2001-09-27 | 2003-04-03 | Functional Genetics, Inc. | Methods and compositions for gene targeting by homologous recombination |
DE50115580D1 (de) † | 2001-10-01 | 2010-09-16 | Deutsches Krebsforsch | Verfahren zur Herstellung von Protein-Bibliotheken und zur Selektion von Proteinen daraus |
US20030135872A1 (en) * | 2001-12-04 | 2003-07-17 | Burgess Robert M. | Gene targeting methods and vectors |
AU2003231244A1 (en) * | 2002-05-02 | 2003-11-17 | University Of North Carolina At Chapel Hill | In vitro mutagenesis, phenotyping, and gene mapping |
FR2860517B1 (fr) * | 2003-10-03 | 2006-02-10 | Evologic Sa | Procedes et outils pour la modification chromosomique de bacteries |
EP1859037A2 (en) | 2005-03-08 | 2007-11-28 | BASF Plant Science GmbH | Expression enhancing intron sequences |
JP5087625B2 (ja) | 2006-09-01 | 2012-12-05 | セラピューティック・ヒューマン・ポリクローナルズ・インコーポレーテッド | 非ヒトトランスジェニック動物におけるヒトまたはヒト化免疫グロブリンの発現強化 |
UA100692C2 (ru) | 2007-05-02 | 2013-01-25 | Мериал Лимитед | Днк-плазмиды, имеющие повышенную экспрессию и стабильность |
WO2009130598A1 (en) * | 2008-04-21 | 2009-10-29 | Unitargeting Research As | Genetically modified eukaryotic cells |
CN102618453A (zh) * | 2011-10-31 | 2012-08-01 | 四川农业大学 | 猪传染性胸膜肺炎放线杆菌Apx I C基因缺失突变株、构建方法、疫苗及应用 |
JP6037290B2 (ja) | 2011-12-13 | 2016-12-07 | 公立大学法人横浜市立大学 | 遺伝子ターゲティングベクター及びその利用方法 |
DE102014116334A1 (de) * | 2014-11-10 | 2016-05-12 | Eberhard Karls Universität Tübingen Medizinische Fakultät | Herstellung von rekombinanten Expressionsvektoren |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH01203975A (ja) * | 1988-02-09 | 1989-08-16 | Konica Corp | 流体試料中の特定成分の測定方法 |
DE3907679A1 (de) * | 1989-03-09 | 1990-09-13 | Max Planck Gesellschaft | Verfahren zur gezielten veraenderung eines gens |
US5464764A (en) | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
EP0747485B1 (en) † | 1989-11-06 | 1998-12-02 | Cell Genesys, Inc. | Production of proteins using homologous recombination |
CA2040099A1 (en) | 1990-05-01 | 1991-11-02 | Mariano Barbacid | Tyrosine kinase negative trkb |
EP0559834A1 (en) * | 1990-11-30 | 1993-09-15 | Abbott Laboratories | Immunoassay and monoclonal antibodies useful for detecting truncated nerve growth factor receptor |
US5641670A (en) * | 1991-11-05 | 1997-06-24 | Transkaryotic Therapies, Inc. | Protein production and protein delivery |
PT101031B (pt) † | 1991-11-05 | 2002-07-31 | Transkaryotic Therapies Inc | Processo para o fornecimento de proteinas por terapia genetica |
ES2240962T3 (es) * | 1993-06-04 | 2005-10-16 | The United States Of America As Represented By The Secretary Of The Navy | Metodo para estimular selectivamente la proliferacion de celulas t. |
IT1261847B (it) | 1993-09-01 | 1996-06-03 | San Romanello Centro Fond | Metodo per marcare cellule eucariote. |
US6066322A (en) * | 1995-03-03 | 2000-05-23 | Millennium Pharmaceuticals, Inc. | Methods for the treatment of immune disorders |
US5633161A (en) * | 1995-03-29 | 1997-05-27 | Millennium Pharmaceuticals, Inc. | Murine gene fomy030 coding for tumor progression inhibitor |
US6017754A (en) * | 1995-08-24 | 2000-01-25 | Invitrogen Corporation | System for isolating and identifying eukaryotic cells transfected with genes and vectors, host cells and methods thereof |
-
1998
- 1998-10-19 ZA ZA9809497A patent/ZA989497B/xx unknown
- 1998-10-20 CA CA2306229A patent/CA2306229C/en not_active Expired - Lifetime
- 1998-10-20 AR ARP980105211A patent/AR017368A1/es active IP Right Grant
- 1998-10-20 EP EP98955483A patent/EP1025253B2/de not_active Expired - Lifetime
- 1998-10-20 PT PT98955483T patent/PT1025253E/pt unknown
- 1998-10-20 AT AT98955483T patent/ATE260982T1/de active
- 1998-10-20 BR BRPI9813099-4A patent/BR9813099B1/pt not_active IP Right Cessation
- 1998-10-20 DE DE59810925T patent/DE59810925D1/de not_active Expired - Lifetime
- 1998-10-20 WO PCT/EP1998/006616 patent/WO1999020780A1/de not_active Application Discontinuation
- 1998-10-20 TR TR2000/01046T patent/TR200001046T2/xx unknown
- 1998-10-20 US US09/509,560 patent/US6284541B1/en not_active Expired - Lifetime
- 1998-10-20 DK DK98955483T patent/DK1025253T4/da active
- 1998-10-20 ES ES98955483T patent/ES2213925T5/es not_active Expired - Lifetime
- 1998-10-20 JP JP2000517100A patent/JP4417549B2/ja not_active Expired - Lifetime
- 1998-10-20 AU AU12293/99A patent/AU730466B2/en not_active Expired
- 1998-10-20 KR KR1020007004264A patent/KR100566702B1/ko not_active IP Right Cessation
- 1998-10-20 CN CNB98810394XA patent/CN1146666C/zh not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
CN1146666C (zh) | 2004-04-21 |
DE59810925D1 (de) | 2004-04-08 |
TR200001046T2 (tr) | 2001-07-23 |
JP2001523442A (ja) | 2001-11-27 |
CA2306229C (en) | 2011-03-22 |
EP1025253B2 (de) | 2009-07-29 |
BR9813099B1 (pt) | 2010-02-09 |
CA2306229A1 (en) | 1999-04-29 |
CN1276836A (zh) | 2000-12-13 |
AU730466B2 (en) | 2001-03-08 |
EP1025253B1 (de) | 2004-03-03 |
DK1025253T3 (da) | 2004-07-12 |
DK1025253T4 (da) | 2009-08-31 |
ZA989497B (en) | 2000-04-19 |
US6284541B1 (en) | 2001-09-04 |
WO1999020780A1 (de) | 1999-04-29 |
PT1025253E (pt) | 2004-07-30 |
AR017368A1 (es) | 2001-09-05 |
ES2213925T5 (es) | 2009-11-11 |
ES2213925T3 (es) | 2004-09-01 |
ATE260982T1 (de) | 2004-03-15 |
JP4417549B2 (ja) | 2010-02-17 |
KR20010031277A (ko) | 2001-04-16 |
KR100566702B1 (ko) | 2006-04-03 |
AU1229399A (en) | 1999-05-10 |
EP1025253A1 (en) | 2000-08-09 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BR9813099A (pt) | Seleção positiva-negativa na recombinação homóloga | |
BR9815855A (pt) | Genes e métodos para controle de nematóides em plantas | |
DE69636288D1 (de) | Samenstrenung resistenz | |
SG147468A1 (en) | High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences | |
PT1428878E (pt) | Processo para a produção e purificação de eritropoietina | |
BR9809433A (pt) | Moléculas de dna enzimáticas | |
PE20040900A1 (es) | Acidos nucleicos inmunoestimuladores | |
AR044724A1 (es) | Promotores del virus de la rizadura amarilla del cestrum | |
AR005568A1 (es) | Moleculas y construcciones de adn para aumentar la expresion de genes foraneos; celulas y plantas transformadas y metodo para producirlas | |
DE69534855D1 (de) | Enzymaktive dna moleküle | |
BR0104520B1 (pt) | processo para a produção de uma montagem de eletrodo de membrana para células de combustìvel. | |
BRPI9811031B8 (pt) | processo para preparar uma célula produtora de epo humana, processo para preparar epo humana, construção de dna para ativação de gene de epo endógeno, bem como plasmìdio. | |
PL1608752T3 (pl) | Sposób izolacji kwasów nukleinowych | |
BR0313455A (pt) | modulação "antisense" da expressão de nav1.3 | |
AR043862A1 (es) | Procedimiento para la obtencion de estirpes celulares de mastocitos a partir de tejidos de cerdo y procedimiento para la produccion de moleculas de tipo de la heparina | |
BR9811853A (pt) | Processos para aumentar o rendimento em plantas | |
WO2003089617A3 (en) | Methods for increasing homologous recombination of a nucleic acid sequence | |
BR0316822A (pt) | Recombinação de dna especìfica da sequência em células eucarióticas | |
EA200101093A1 (ru) | Экспрессия гепарин-связывающего протеина в рекомбинантных клетках млекопитающих | |
WO2001073001A3 (en) | Establishment of cellular manipulations which enhance oligo-mediated gene targeting | |
AR016839A1 (es) | Gen que codifica la androctonina, vector que lo contiene y plantas transformadas obtenidas resistentes a enfermedades | |
AR016264A1 (es) | Construccion de adn que altera la expresion de un gen endogeno de ifna2 en celulas de mamifero al integrarse al genoma de la celula por medio derecombinacion homologa, celula homologamente recombinante, metodo para alterar la expresion del gen endogeno de ifn2 en una celula de mamifero, uso dedicha | |
PT1537210E (pt) | Inibicao de oliconucleotidos decoy de expressao cd-40 | |
SE9801245D0 (sv) | Genetic recombination | |
WO2001059139A3 (de) | Ozon- und/oder pathogen-induzierbare genexpression in pflanzen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 09/02/2010, OBSERVADAS AS CONDICOES LEGAIS. |
|
B21A | Patent or certificate of addition expired [chapter 21.1 patent gazette] |
Free format text: PATENTE EXTINTA EM 09.02.2020 |