AR005568A1 - Moleculas y construcciones de adn para aumentar la expresion de genes foraneos; celulas y plantas transformadas y metodo para producirlas - Google Patents

Moleculas y construcciones de adn para aumentar la expresion de genes foraneos; celulas y plantas transformadas y metodo para producirlas

Info

Publication number
AR005568A1
AR005568A1 ARP970100323A ARP970100323A AR005568A1 AR 005568 A1 AR005568 A1 AR 005568A1 AR P970100323 A ARP970100323 A AR P970100323A AR P970100323 A ARP970100323 A AR P970100323A AR 005568 A1 AR005568 A1 AR 005568A1
Authority
AR
Argentina
Prior art keywords
expression
foreign genes
cells
molecules
produce
Prior art date
Application number
ARP970100323A
Other languages
English (en)
Original Assignee
Univ North Carolina State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina State filed Critical Univ North Carolina State
Publication of AR005568A1 publication Critical patent/AR005568A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression

Abstract

Se describe una región de unión a la matriz o soporte nuclear aislada de un gen del tabaco y un método para producir células recombinantes que poseenniveles aumentados de expresión de los genes extranos contenidos en las mismas. El métodocomprend e la transformación de una célula con una construcción deADN que comprende, en la dirección 5 a 3, una región de iniciación de la transcripción, un gen estructural ubicado hacia el extremo 3 con respecto a laregión de iniciación de latranscripc ión y que está asociado operativamente con la misma y la región de unión al soporte nuclear de la SEQ ID N° 1 ubicadahacia el extremo 5 con respecto a dicha región de iniciación de la transcripción o bien, hacia el extremo 3con respecto a dicho gen estructural.También se discuten las construcciones de ADN y los vectoes empleados para llevar a cabo el método recién mencionado.
ARP970100323A 1996-01-26 1997-01-27 Moleculas y construcciones de adn para aumentar la expresion de genes foraneos; celulas y plantas transformadas y metodo para producirlas AR005568A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/592,658 US5773695A (en) 1996-01-26 1996-01-26 Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells

Publications (1)

Publication Number Publication Date
AR005568A1 true AR005568A1 (es) 1999-06-23

Family

ID=24371559

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP970100323A AR005568A1 (es) 1996-01-26 1997-01-27 Moleculas y construcciones de adn para aumentar la expresion de genes foraneos; celulas y plantas transformadas y metodo para producirlas

Country Status (10)

Country Link
US (1) US5773695A (es)
EP (1) EP0904276B1 (es)
JP (1) JP4709334B2 (es)
AR (1) AR005568A1 (es)
AT (1) ATE421591T1 (es)
AU (1) AU716202B2 (es)
BR (1) BR9707208A (es)
DE (1) DE69739231D1 (es)
ES (1) ES2318855T3 (es)
WO (1) WO1997027207A1 (es)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239328B1 (en) 1992-10-05 2001-05-29 North Carolina State University Method for reducing expression variability of transgenes in plant cells
BR9807899A (pt) * 1997-03-28 2000-02-22 Mycogen Plant Science Inc Processo de transformação de plantas por sar aperfeiçoado
WO1998055608A1 (en) * 1997-06-03 1998-12-10 North Carolina State University Method for reducing expression variability of transgenes in plants cells
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
WO2000006757A1 (en) * 1998-07-31 2000-02-10 Mycogen Plant Science, Inc. Improved plant transformation process by scaffold attachment regions (sar)
US6177612B1 (en) 1998-07-31 2001-01-23 Her Majesty The Queen In Right Of Canada, As Represented By The Department Of Agriculture And Agri-Food Canada Matrix attachment regions
CA2343080A1 (en) * 1998-09-29 2000-04-06 Pioneer Hi-Bred International, Inc. Mar/sar elements flanking rsyn7-driven construct
JP4544748B2 (ja) * 1998-12-01 2010-09-15 ダウ・アグロサイエンス・エル・エル・シー 植物細胞内に導入された遺伝子の発現を増加するための人工マトリックス付着領域
DE19857654A1 (de) 1998-12-14 2000-06-15 Max Planck Gesellschaft Beeinflussung des Blühverhaltens von Pflanzen durch Expression Saccharose-spaltender Proteine
WO2000077176A1 (en) * 1999-06-14 2000-12-21 North Carolina State University Method for obtaining low copy transgenes by direct dna transformation
US7126041B1 (en) 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
SG141239A1 (en) * 2001-01-26 2008-04-28 Selexis Sa Matrix attachment regions and methods for use thereof
MXPA03011801A (es) * 2001-07-04 2005-03-07 Chromagenics Bv Secuencias de acido desoxirribonucleico (adn) que comprenden cualidades reguladoras de transcripcion de genes y metodos para deteccion y uso de secuencias de acido desoxirribonucleico (adn).
CA2427190A1 (en) * 2002-04-30 2003-10-30 Alberta Research Council Inc. Production of recombinant epidermal growth factor in plants
JP4647309B2 (ja) * 2002-06-14 2011-03-09 クロマジェニックス ビー．ブイ． 複数のタンパク質の同時生産のための方法；そこで使用するためのベクター及び細胞
WO2003106674A2 (en) * 2002-06-14 2003-12-24 Chromagenics B.V. Means and methods for regulating gene expression
US20040016014A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US20040016015A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
WO2004055215A1 (en) * 2002-12-18 2004-07-01 Chromagenics B.V. A method for improving protein production
AU2003290453A1 (en) * 2002-12-20 2004-07-14 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
CN100480261C (zh) * 2003-02-26 2009-04-22 中国科学院上海生命科学研究院 核基质蛋白结合序列及其应用
AP2265A (en) * 2003-08-19 2011-08-01 22Nd Century Ltd Llc Reduced-exposure tobacco products.
EP2292754B1 (en) 2003-10-24 2012-12-26 Selexis S.A. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
NZ553700A (en) 2004-11-08 2010-09-30 Chromagenics Bv Selection of host cells expressing protein at high levels using a selectable marker with a non-optimal start codon
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
CA2581422C (en) * 2004-11-08 2012-05-22 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US7553666B2 (en) 2005-11-04 2009-06-30 Dow Agrosciences Llc Preparation of vaccine master cell lines using recombinant plant suspension cultures
KR101439568B1 (ko) 2006-08-11 2014-09-12 다우 아그로사이언시즈 엘엘씨 아연 손가락 뉴클레아제-매개 상동 재조합
KR101520507B1 (ko) 2006-12-14 2015-05-29 다우 아그로사이언시즈 엘엘씨 최적화된 비-정규 아연 손가락 단백질
JP2010514698A (ja) * 2006-12-22 2010-05-06 ダウ・アグロサイエンス・エル・エル・シー 植物によって製造されるウエストナイルウイルス（ｗｎｖ）ワクチン、ベクターおよび植物コドン最適化配列
EP2610345B1 (en) 2007-11-27 2015-08-19 Medicago Inc. Recombinant influenza virus-like particles (VLPS) produced in transgenic plants expressing hemagglutinin
DK2480560T3 (en) * 2009-09-22 2018-04-16 Medicago Inc PROCEDURE FOR THE PREPARATION OF PLANT DERIVATIVE PROTEINS
BR122019025207B1 (pt) 2009-10-22 2022-10-04 Sangamo Biosciences, Inc. Proteína de dedo de zinco de ocorrência não natural e proteína de fusão
CN102843904B (zh) 2010-01-22 2015-01-21 陶氏益农公司 在经遗传修饰的生物体中切除转基因
WO2012033794A2 (en) * 2010-09-08 2012-03-15 Dow Agrosciences Llc Aad-12 event 1606 and related transgenic soybean lines
CA2820706C (en) 2010-12-03 2018-05-22 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
NZ628549A (en) 2012-02-01 2016-10-28 Dow Agrosciences Llc Novel class of glyphosate resistance genes
TW201538518A (zh) 2014-02-28 2015-10-16 Dow Agrosciences Llc 藉由嵌合基因調控元件所賦予之根部特異性表現
EP3782465B1 (en) 2019-08-23 2023-08-30 Universität Potsdam Transgenic zebrafish model for stroke

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5459252A (en) * 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
ES2176208T3 (es) * 1992-10-05 2002-12-01 Univ North Carolina State Metodo para incrementar la expresion y para reducir la variabilidad de expresion de genes foraneos en celulas vegetales.

Also Published As

Publication number Publication date
JP4709334B2 (ja) 2011-06-22
US5773695A (en) 1998-06-30
DE69739231D1 (de) 2009-03-12
ATE421591T1 (de) 2009-02-15
AU716202B2 (en) 2000-02-24
EP0904276A4 (en) 2004-03-31
EP0904276A1 (en) 1999-03-31
EP0904276B1 (en) 2009-01-21
WO1997027207A1 (en) 1997-07-31
JP2000504222A (ja) 2000-04-11
AU2246997A (en) 1997-08-20
BR9707208A (pt) 1999-04-06
ES2318855T3 (es) 2009-05-01

Similar Documents

Publication Publication Date Title
AR005568A1 (es) Moleculas y construcciones de adn para aumentar la expresion de genes foraneos; celulas y plantas transformadas y metodo para producirlas
NZ333998A (en) Method for reducing expression variability of transgenes in plant cells using DNA constructs comprising insulator elements
BR9815855A (pt) Genes e métodos para controle de nematóides em plantas
AR003112A1 (es) Un metodo para controlar la expresion de un gen estructural en una celula, construcciones de adn para uso en dicho metodo y capaz de alterar la expresionde un gen codificante de trombopoyetina, adnasa o interferon beta, molecula de adn aislado, celulas homologamente recombinantes, metodos para producir dichas
ES2056799T3 (es) Moleculas de adn codante para las regiones de control fmdh y gen estructural para una proteina que tiene una actividad de fmdh y su uso.
DK0941318T3 (da) Håndvægtformede konstruerede ekspressionssystemer til genterapi
MX9304027A (es) Variante de al enzima lipasa y metodo para su produccion.
ATE348891T1 (de) Wurzelrinden spezifischer genpromoter
ATE184649T1 (de) Gezielte spaltung von rns mittels gezielter bindung der ribonuklease p und spaltungssequenzen
MX428A (es) Region regulatoria para expresion de gen heterologa en levadura.
ATE526818T1 (de) Gentechnisch hergestellte wasserlinse
ATE329030T1 (de) Nukleinsäure, welche einen von lektin abstammenden faktor zur präservierung von progenitorzellen kodiert
AR020331A1 (es) Construccion, y metodo para producir una proteina en una celula de planta
ES2123008T3 (es) Secuencias de adn que codifican polipeptido de gelonina.
BR9912498A (pt) Expressão de peptìdios eucarióticos em plastìdio de plantas
WO1997026322A3 (en) Methods and compositions for inhibiting hexokinase
DE60144021D1 (de) Für zellzyklusproteine kodierende nukleinsäure und deren verwendung
AR027765A1 (es) Expresion y purificacion de polipeptidos bioactivos autenticos a partir de plantas
BR9813099A (pt) Seleção positiva-negativa na recombinação homóloga
ATE369430T1 (de) Methoden zur herstellung von nukleotidintegrasen
AR043862A1 (es) Procedimiento para la obtencion de estirpes celulares de mastocitos a partir de tejidos de cerdo y procedimiento para la produccion de moleculas de tipo de la heparina
AR016262A1 (es) Una construccion de adn que altera la expresion de un gen pshbeta endogeno, una celula homologamente recombinante, un metodo para alterar la expresion dela fshbeta endogeno en la celula de un mamifero, uso de dicha celula para la manufactura de un medicamento, un metodo para producir fshbeta y una m
CO4480058A1 (es) Genes codificantes y procedimiento de expresion de una nueva enzima: ftalil amidasa
BR9712532A (pt) Método para aumentar o nìvel de expressão de um transgene população de células monocotiledÈneas,plantas e sementes
ES2109194B1 (es) Un procedimiento para producir la enzima d-aminoacido oxidasa de rhodotorula gracilis en celulas hospedantes.

Legal Events

Date Code Title Description
FB Suspension of granting procedure