BR9713108A - Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células - Google Patents

Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células

Info

Publication number
BR9713108A
BR9713108A BR9713108-3A BR9713108A BR9713108A BR 9713108 A BR9713108 A BR 9713108A BR 9713108 A BR9713108 A BR 9713108A BR 9713108 A BR9713108 A BR 9713108A
Authority
BR
Brazil
Prior art keywords
cell
collagenase
histolytucum
type
recombinant clostridium
Prior art date
Application number
BR9713108-3A
Other languages
English (en)
Portuguese (pt)
Inventor
Helmut Burtscher
Dorothee Ambrosius
Friederike Hesse
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of BR9713108A publication Critical patent/BR9713108A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
BR9713108-3A 1996-11-19 1997-10-30 Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células BR9713108A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP96118490 1996-11-19
EP97101085 1997-01-24
PCT/EP1997/006002 WO1998022574A2 (fr) 1996-11-19 1997-10-30 Collagenase recombinee type i provenant du clostridium histolyticum, et son utilisation pour l'isolation des cellules et des agglomerations cellulaires

Publications (1)

Publication Number Publication Date
BR9713108A true BR9713108A (pt) 2004-06-22

Family

ID=26142307

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9713108-3A BR9713108A (pt) 1996-11-19 1997-10-30 Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células

Country Status (9)

Country Link
US (1) US6475764B1 (fr)
EP (1) EP0941311B1 (fr)
JP (1) JP2001510331A (fr)
AT (1) ATE272109T1 (fr)
AU (1) AU5222498A (fr)
BR (1) BR9713108A (fr)
CA (1) CA2272244A1 (fr)
DE (1) DE59711812D1 (fr)
WO (1) WO1998022574A2 (fr)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280993B1 (en) * 1999-08-24 2001-08-28 Ichiro Yamato Gene encoding class I collagenase
US7651684B2 (en) 2001-12-07 2010-01-26 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
US7771716B2 (en) 2001-12-07 2010-08-10 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of musculoskeletal disorders
US7585670B2 (en) 2001-12-07 2009-09-08 Cytori Therapeutics, Inc. Automated methods for isolating and using clinically safe adipose derived regenerative cells
US20050095228A1 (en) 2001-12-07 2005-05-05 Fraser John K. Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders
AU2002357135C1 (en) 2001-12-07 2009-01-22 Macropore Biosurgery, Inc. Systems and methods for treating patients with processed lipoaspirate cells
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US8105580B2 (en) 2001-12-07 2012-01-31 Cytori Therapeutics, Inc. Methods of using adipose derived stem cells to promote wound healing
EP1433845A1 (fr) * 2002-12-23 2004-06-30 Georg-August-Universität Collagenase de Clostridium tetani et utilisations
ATE524070T1 (de) 2003-02-20 2011-09-15 Cytori Therapeutics Inc Verfahren zur verwendung von aus fettgewebe stammenden zellen bei der behandlung von herz- kreislauf-leiden
DE10331171A1 (de) * 2003-07-09 2005-01-27 Nordmark Arzneimittel Gmbh & Co. Kg Neutrale Protease (NP) und Produkt unter Verwendung von neutraler Protease für Gewebedissoziation sowie Verfahren zu dessen Herstellung
ES2403357T3 (es) 2003-12-11 2013-05-17 Isto Technologies Inc. Sistema de cartílago particulado
US20070231788A1 (en) * 2003-12-31 2007-10-04 Keyvan Behnam Method for In Vitro Assay of Demineralized Bone Matrix
JP5292533B2 (ja) 2005-08-26 2013-09-18 ジンマー・インコーポレイテッド インプラントおよび関節疾患の治療、置換および治療方法
US7811560B2 (en) * 2006-01-30 2010-10-12 Auxilium Us Holdings, Llc Compositions and methods for treating collagen-mediated diseases
US8323642B2 (en) * 2006-12-13 2012-12-04 Depuy Mitek, Inc. Tissue fusion method using collagenase for repair of soft tissue
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
US20080233614A1 (en) * 2007-02-13 2008-09-25 Cranenburgh Rocky M Production of recombinant collagenases colg and colh in escherichia coli
WO2008128075A1 (fr) 2007-04-12 2008-10-23 Isto Technologies, Inc. Compositions et procédés de réparation de tissu
US20100159564A1 (en) * 2007-11-30 2010-06-24 Dwulet Francis E Protease resistant recombinant bacterial collagenases
EP2130551B1 (fr) * 2008-06-02 2012-06-06 Roche Diagnostics GmbH Purification améliorée de collagénases à partir de culture liquide de Clostridium histolyticum
WO2010021993A1 (fr) 2008-08-19 2010-02-25 Cytori Therapeutics, Inc. Procédés d'utilisation de cellules issues du tissu adipeux dans le traitement du système lymphatique et d'une maladie maligne
KR101644534B1 (ko) 2009-05-01 2016-08-01 비미니 테크놀로지스 엘엘씨 조직 및 세포 부유화 이식편의 최적화 시스템, 방법 및 조성물
DK2802652T3 (da) * 2012-01-12 2019-07-15 Endo Global Ventures Clostridium histolyticum enzym
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
KR20160055827A (ko) 2013-09-19 2016-05-18 사이토리 테라퓨틱스, 인크. 통증 및/또는 섬유증의 조절에 지방 조직-유래 세포를 사용하는 방법
JP6661072B2 (ja) 2014-03-06 2020-03-11 株式会社ニッピ 細胞分離用酵素剤
JP7558654B2 (ja) 2017-03-01 2024-10-01 エンド ベンチャーズ アンリミテッド カンパニー セルライトを評価及び処置するための装置及び方法
KR20240001279A (ko) * 2017-03-28 2024-01-03 엔도 벤쳐즈 리미티드 개선된 콜라게나제 생성 방법
WO2020174005A1 (fr) 2019-02-28 2020-09-03 The Regenerative Group Ltd. Combinaison régénérative de plasma et de tissu adipeux
EP4130277A4 (fr) 2020-03-31 2024-05-01 Amano Enzyme Inc. Agent de collagénase et son application d'utilisation
CN113025599B (zh) * 2021-04-02 2023-09-12 重庆科润生物医药研发有限公司 一种重组溶组织梭菌i型胶原酶及其制备方法和应用
CN112941058B (zh) * 2021-04-02 2023-12-05 重庆科润生物医药研发有限公司 一种重组溶组织梭菌ii型胶原酶及其制备方法和应用
CN118159651A (zh) 2021-09-28 2024-06-07 天野酶制品株式会社 用于制造胶原三肽或者分解软骨的酶剂

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801038A (en) * 1984-05-29 1998-09-01 Genencor International Inc. Modified subtilisins having amino acid alterations
US5177011A (en) 1988-08-18 1993-01-05 Calgene, Inc. Plant elongation factor promoters, coding sequences and uses
US5177017A (en) * 1990-03-22 1993-01-05 Trigen, Inc. Molecular cloning of the genes responsible for collagenase production from Clostridium histolyticum
JPH08500970A (ja) 1992-06-22 1996-02-06 トライジェン，インコーポレーテッド ヒストリチクス菌からのコラゲナーゼ産生に応答性の遺伝子の分子クローニング
DE4408940A1 (de) * 1994-03-16 1995-10-05 Boehringer Mannheim Gmbh Rekombinante Collagenase Typ II aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden

Also Published As

Publication number Publication date
WO1998022574A3 (fr) 1998-07-09
EP0941311A2 (fr) 1999-09-15
CA2272244A1 (fr) 1998-05-28
ATE272109T1 (de) 2004-08-15
EP0941311B1 (fr) 2004-07-28
JP2001510331A (ja) 2001-07-31
DE59711812D1 (de) 2004-09-02
US6475764B1 (en) 2002-11-05
AU5222498A (en) 1998-06-10
WO1998022574A2 (fr) 1998-05-28

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1905 DE 10/07/2007.