BR112022014567A2 - Métodos de purificação - Google Patents

Métodos de purificação

Info

Publication number
BR112022014567A2
BR112022014567A2 BR112022014567A BR112022014567A BR112022014567A2 BR 112022014567 A2 BR112022014567 A2 BR 112022014567A2 BR 112022014567 A BR112022014567 A BR 112022014567A BR 112022014567 A BR112022014567 A BR 112022014567A BR 112022014567 A2 BR112022014567 A2 BR 112022014567A2
Authority
BR
Brazil
Prior art keywords
methods
purification methods
purification
supernator
interleukin
Prior art date
Application number
BR112022014567A
Other languages
English (en)
Inventor
R Mcneill Blair
Prabu Vijayakumar Saraswathi Raj
j pete Matthew
Original Assignee
Alkermes Pharma Ireland Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alkermes Pharma Ireland Ltd filed Critical Alkermes Pharma Ireland Ltd
Publication of BR112022014567A2 publication Critical patent/BR112022014567A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
    • B01D15/245—Adding materials to the effluents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Removal Of Specific Substances (AREA)
  • Cephalosporin Compounds (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)

Abstract

MÉTODOS DE PURIFICAÇÃO DE UM POLIPEPTÍDEO, PARA REDUZIR O TEOR DE PROTEÍNA DE CÉLULA HOSPEDEIRA DE UM SOBRENADANTE DE CÉLULA E PARA MELHORAR A MEIA VIDA SÉRICA DE UMA COMPOSIÇÃO E COMPOSIÇÕES. São fornecidos na presente invenção métodos de purificação de polipeptídeos compreendendo uma interleucina-2 (IL-2) permutada circularmente fundida com a porção extracelular de uma cadeia de IL-2Ra.
BR112022014567A 2020-01-24 2021-01-21 Métodos de purificação BR112022014567A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202062965578P 2020-01-24 2020-01-24
PCT/US2021/014306 WO2021150674A1 (en) 2020-01-24 2021-01-21 Methods of purification

Publications (1)

Publication Number Publication Date
BR112022014567A2 true BR112022014567A2 (pt) 2022-09-27

Family

ID=76992521

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022014567A BR112022014567A2 (pt) 2020-01-24 2021-01-21 Métodos de purificação

Country Status (12)

Country Link
US (1) US20210253631A1 (pt)
EP (1) EP4093755A1 (pt)
JP (1) JP2023511946A (pt)
KR (1) KR20220143676A (pt)
CN (1) CN115335394A (pt)
AU (1) AU2021210905A1 (pt)
BR (1) BR112022014567A2 (pt)
CA (1) CA3165442A1 (pt)
IL (1) IL294901A (pt)
MX (1) MX2022009134A (pt)
TW (1) TW202140512A (pt)
WO (1) WO2021150674A1 (pt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20230009446A (ko) * 2020-05-11 2023-01-17 엘커메스 파마 아일랜드 리미티드 Il-2 융합 폴리펩티드 조성물 및 그의 제조 및 사용 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200611910A (en) * 2004-05-19 2006-04-16 Maxygen Inc Interferon-alpha polypeptides and conjugates
ME03079B (me) * 2012-06-08 2019-01-20 Alkermes Pharma Ireland Ltd Ligandi modifikovani cirkularnom permutacijom као agonisтi i antagonisti
MX2018005834A (es) * 2015-11-18 2018-08-01 Merck Patent Gmbh Separacion de proteina mejorada en cromatografia de intercambio de ion.

Also Published As

Publication number Publication date
JP2023511946A (ja) 2023-03-23
US20210253631A1 (en) 2021-08-19
AU2021210905A1 (en) 2022-09-01
EP4093755A1 (en) 2022-11-30
WO2021150674A1 (en) 2021-07-29
MX2022009134A (es) 2022-10-21
IL294901A (en) 2022-09-01
CN115335394A (zh) 2022-11-11
KR20220143676A (ko) 2022-10-25
TW202140512A (zh) 2021-11-01
CA3165442A1 (en) 2021-07-29

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