BR112021021859A2 - Aminoacil-trna sintetase introduzindo derivados de lisina de forma eficaz - Google Patents

Aminoacil-trna sintetase introduzindo derivados de lisina de forma eficaz

Info

Publication number
BR112021021859A2
BR112021021859A2 BR112021021859A BR112021021859A BR112021021859A2 BR 112021021859 A2 BR112021021859 A2 BR 112021021859A2 BR 112021021859 A BR112021021859 A BR 112021021859A BR 112021021859 A BR112021021859 A BR 112021021859A BR 112021021859 A2 BR112021021859 A2 BR 112021021859A2
Authority
BR
Brazil
Prior art keywords
trna synthetase
aminoacyl
amino acid
lysine derivatives
effectively introducing
Prior art date
Application number
BR112021021859A
Other languages
English (en)
Inventor
Huiling Liu
Song Wu
Wei Chen
Zhenshan Zhang
Original Assignee
Suzhou Kunpeng Biotech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Suzhou Kunpeng Biotech Co Ltd filed Critical Suzhou Kunpeng Biotech Co Ltd
Publication of BR112021021859A2 publication Critical patent/BR112021021859A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01006—Lysine-tRNA ligase (6.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

aminoacil-trna sintetase introduzindo derivados de lisina de forma eficaz. é fornecido pela presenta invenção uma lisil-trna sintetase mutante, a qual é eliminada em um ou mais sítios selecionados a partir do seguinte grupo correspondente à sequência de aminoácidos de aminoacil-trna sintetase de tipo selvagem: 102º, 128º-140º e 159º-179º resíduos de aminoácidos. comparado com lisil-trna sintetase de tipo selvagem, a lisil-trna sintetase mutante de acordo com a presente invenção possui alta atividade, um alto nível de expressão e boa solubilidade, e pode aumentar significativamente a quantidade de um aminoácido não natural inserido e o nível de expressão de uma proteína alvo que contém um aminoácido não natural.
BR112021021859A 2019-04-30 2020-04-30 Aminoacil-trna sintetase introduzindo derivados de lisina de forma eficaz BR112021021859A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201910364788.7A CN111849929B (zh) 2019-04-30 2019-04-30 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶
PCT/CN2020/088506 WO2020221365A1 (zh) 2019-04-30 2020-04-30 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶

Publications (1)

Publication Number Publication Date
BR112021021859A2 true BR112021021859A2 (pt) 2021-12-21

Family

ID=72965094

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021021859A BR112021021859A2 (pt) 2019-04-30 2020-04-30 Aminoacil-trna sintetase introduzindo derivados de lisina de forma eficaz

Country Status (8)

Country Link
US (1) US20220290202A1 (pt)
EP (1) EP3964570A4 (pt)
JP (2) JP2022530917A (pt)
CN (2) CN111849929B (pt)
AU (1) AU2020265979A1 (pt)
BR (1) BR112021021859A2 (pt)
CA (1) CA3138814A1 (pt)
WO (1) WO2020221365A1 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111850020B (zh) * 2019-04-25 2021-05-07 苏州鲲鹏生物技术有限公司 利用质粒系统在蛋白中引入非天然氨基酸
CN111849929B (zh) * 2019-04-30 2021-05-11 苏州鲲鹏生物技术有限公司 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶
CN115701451B (zh) * 2021-08-02 2023-08-01 宁波鲲鹏生物科技有限公司 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用
CA3230774A1 (en) * 2021-09-06 2023-03-09 Veraxa Biotech Gmbh Novel aminoacyl-trna synthetase variants for genetic code expansion in eukaryotes
CN114134141B (zh) * 2021-11-03 2024-01-30 杭州嵌化合生医药科技有限公司 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法
CN114657156A (zh) * 2022-03-11 2022-06-24 南京中医药大学 吡咯赖氨酸氨酰-tRNA合成酶突变体及其筛选系统和应用
CN117279889A (zh) * 2022-04-28 2023-12-22 深圳湾实验室 经取代的氟硫酸盐及其用途
CN114908066B (zh) * 2022-05-17 2024-01-23 杭州嵌化合生医药科技有限公司 一种正交翻译系统及其在再分配密码子恢复ptc疾病中功能蛋白表达方面的应用

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0817212A2 (pt) * 2007-09-20 2014-10-14 Riken Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização
GB2470770A (en) * 2009-06-04 2010-12-08 Medical Res Council Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs
IN2014DN09922A (pt) * 2012-05-18 2015-08-14 Medical Res Council
CN104099360A (zh) * 2013-04-12 2014-10-15 北京大学 非天然氨基酸标记的目的蛋白或肽的制备
EP2894476A1 (en) * 2014-01-14 2015-07-15 Universität Konstanz Genetically Encoded Spin Label
CN104450746A (zh) * 2014-08-30 2015-03-25 武汉佰福泰制药有限公司 一种向蛋白质定点引入非天然氨基酸的方法
GB201419109D0 (en) * 2014-10-27 2014-12-10 Medical Res Council Incorporation of unnatural amino acids into proteins
CN107012121B (zh) * 2016-01-27 2021-01-26 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN107022568B (zh) * 2016-02-01 2020-08-25 北京大学 哺乳动物细胞中高效多点插入非天然氨基酸的系统
CN107177593B (zh) * 2016-03-10 2020-10-23 北京大学 利用优化的基因密码子扩展系统通读提前终止密码子疾病中的截短蛋白
WO2018045376A2 (en) * 2016-09-02 2018-03-08 Ikaria Inc. Functionally modified polypeptides and radiobiosynthesis
EP3309260A1 (en) * 2016-10-14 2018-04-18 European Molecular Biology Laboratory Archaeal pyrrolysyl trna synthetases for orthogonal use
CN107099546B (zh) * 2017-06-07 2019-11-19 胜武（北京）生物科技有限公司 一种调控嵌合抗原受体表达的方法
US11447809B2 (en) * 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
CN111718949B (zh) * 2019-03-19 2021-10-01 宁波鲲鹏生物科技有限公司 利用双质粒系统在蛋白中引入非天然氨基酸
CN111718920B (zh) * 2019-03-19 2021-05-07 宁波鲲鹏生物科技有限公司 在蛋白中高效引入赖氨酸衍生物的氨酰基-tRNA合成酶
CN111850020B (zh) * 2019-04-25 2021-05-07 苏州鲲鹏生物技术有限公司 利用质粒系统在蛋白中引入非天然氨基酸
CN111849929B (zh) * 2019-04-30 2021-05-11 苏州鲲鹏生物技术有限公司 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶

Also Published As

Publication number Publication date
WO2020221365A1 (zh) 2020-11-05
CA3138814A1 (en) 2020-11-05
AU2020265979A1 (en) 2021-12-23
EP3964570A4 (en) 2023-06-28
JP2022530917A (ja) 2022-07-04
EP3964570A1 (en) 2022-03-09
CN111849929A (zh) 2020-10-30
CN113767168A (zh) 2021-12-07
JP2023174662A (ja) 2023-12-08
US20220290202A1 (en) 2022-09-15
CN111849929B (zh) 2021-05-11

Similar Documents

Publication Publication Date Title
BR112021021859A2 (pt) Aminoacil-trna sintetase introduzindo derivados de lisina de forma eficaz
Dunn Key roles of microsymbiont amino acid metabolism in rhizobia-legume interactions
Fisher et al. Uptake of biotin by Chlamydia Spp. through the use of a bacterial transporter (BioY) and a host-cell transporter (SMVT)
BR112021018681A2 (pt) Aminoacil-trna sintetase para introdução eficiente de derivado de lisina em proteína
Kuhla et al. Effect of a high‐protein diet on food intake and liver metabolism during pregnancy, lactation and after weaning in mice
BR112013005140A2 (pt) controle de foco automático que usa dados de estatísticas de imagem com pontuações de foco automático refinadas e grosseiras
BRPI0509832A (pt) rolamento
BR112013030157A2 (pt) dispositivo de mira que tem marcadores radiopacos
EP3508577A3 (en) Modified amadoriase capable of acting on fructosyl hexapeptide
UY32144A (es) Mutantes de ahas resistentes a herbicidas y metodos de uso
BR112013008407A2 (pt) antígenos de clostridium difficile
Küberl et al. Pupylated proteins in Corynebacterium glutamicum revealed by MudPIT analysis
Imami et al. Global impact of Salmonella pathogenicity island 2-secreted effectors on the host phosphoproteome
Fields et al. Campylobacter jejuni CsrA regulates metabolic and virulence associated proteins and is necessary for mouse colonization
Cao et al. Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus
De Palma et al. Changes in muscle cell metabolism and mechanotransduction are associated with myopathic phenotype in a mouse model of collagen VI deficiency
BR112022010065A2 (pt) Terapia de gene da apoe
BR112017026142A2 (pt) composições e métodos para tratar doença sprue celíaca
BR112017017609A2 (pt) proteína de fusão fc, e, composição farmacêutica.
BR112018012055A2 (pt) substrato de transglutaminase (tg) recombinante, método in vitro para marcar uma proteína de interesse, composição farmacêutica ou de diagnóstico e kit de diagnóstico
BR112021023957A2 (pt) Peptídeos
BR112020004536A8 (pt) Controle de posicionamento dinâmico
Singh et al. Phosphoserine aminotransferase has conserved active site from microbes to higher eukaryotes with minor deviations
Mueller et al. Mechanisms for multiple intracellular localization of human mitochondrial proteins
BR112018001967A2 (pt) material de gravação sensível ao calor.