CN111849929A - 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 - Google Patents
高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 Download PDFInfo
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Abstract
Description
最初的残基 | 代表性的取代 | 优选的取代 |
Ala(A) | Val;Leu;Ile | Val |
Arg(R) | Lys;Gln;Asn | Lys |
Asn(N) | Gln;His;Lys;Arg | Gln |
Asp(D) | Glu | Glu |
Cys(C) | Ser | Ser |
Gln(Q) | Asn | Asn |
Glu(E) | Asp | Asp |
Gly(G) | Pro;Ala | Ala |
His(H) | Asn;Gln;Lys;Arg | Arg |
Ile(I) | Leu;Val;Met;Ala;Phe | Leu |
Leu(L) | Ile;Val;Met;Ala;Phe | Ile |
Lys(K) | Arg;Gln;Asn | Arg |
Met(M) | Leu;Phe;Ile | Leu |
Phe(F) | Leu;Val;Ile;Ala;Tyr | Leu |
Pro(P) | Ala | Ala |
Ser(S) | Thr | Thr |
Thr(T) | Ser | Ser |
Trp(W) | Tyr;Phe | Tyr |
Tyr(Y) | Trp;Phe;Thr;Ser | Phe |
Val(V) | Ile;Leu;Met;Phe;Ala | Leu |
酶 | 丁炔氧羰基人胰岛素的产量(mg/L发酵液) |
野生型pylRs | 530 |
suppylRs | 720 |
IPYEpylRs | 700 |
optpylRs | 730 |
IPYEpylRs(L274A,C313S,F349Y) | 1050 |
单质粒IPYEpylRs | 590 |
Claims (10)
Priority Applications (10)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910364788.7A CN111849929B (zh) | 2019-04-30 | 2019-04-30 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
CA3138814A CA3138814A1 (en) | 2019-04-30 | 2020-04-30 | Aminoacyl-trna synthetase efficiently introducing lysine derivatives |
EP20798501.1A EP3964570A4 (en) | 2019-04-30 | 2020-04-30 | Aminoacyl-trna synthetase efficiently introducing lysine derivatives |
US17/594,841 US20220290202A1 (en) | 2019-04-30 | 2020-04-30 | Aminoacyl-trna synthetase efficiently introducing lysine derivatives |
BR112021021859A BR112021021859A2 (pt) | 2019-04-30 | 2020-04-30 | Aminoacil-trna sintetase introduzindo derivados de lisina de forma eficaz |
PCT/CN2020/088506 WO2020221365A1 (zh) | 2019-04-30 | 2020-04-30 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
AU2020265979A AU2020265979A1 (en) | 2019-04-30 | 2020-04-30 | Aminoacyl-tRNA synthetase efficiently introducing lysine derivatives |
JP2021565005A JP2022530917A (ja) | 2019-04-30 | 2020-04-30 | リシン誘導体を効率的に導入するアミノアシル-tRNAシンテターゼ |
CN202080032606.7A CN113767168A (zh) | 2019-04-30 | 2020-04-30 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
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CA3230774A1 (en) * | 2021-09-06 | 2023-03-09 | Veraxa Biotech Gmbh | Novel aminoacyl-trna synthetase variants for genetic code expansion in eukaryotes |
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CN114908066A (zh) * | 2022-05-17 | 2022-08-16 | 浙江大学 | 一种正交翻译系统及其在再分配密码子恢复ptc疾病中功能蛋白表达方面的应用 |
CN114908066B (zh) * | 2022-05-17 | 2024-01-23 | 杭州嵌化合生医药科技有限公司 | 一种正交翻译系统及其在再分配密码子恢复ptc疾病中功能蛋白表达方面的应用 |
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BR112021021859A2 (pt) | 2021-12-21 |
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