CN113767168A - 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 - Google Patents
高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 Download PDFInfo
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Abstract
本发明提供了一种突变型赖氨酰‑tRNA合成酶，其在对应于野生型氨酰基‑tRNA合成酶的氨基酸序列的选自下组的一个或多个位点发生缺失：第102位、第128‑140位和第159‑179位氨基酸残基。相比野生型赖氨酰‑tRNA合成酶，本发明突变型赖氨酰‑tRNA合成酶活性高，表达量高，可溶性好，可显著提高非天然氨基酸插入量和含有非天然氨基酸的目的蛋白的表达量。
Description
PCT国内申请，说明书已公开。
Claims (10)
- PCT国内申请，权利要求书已公开。
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| CN201910364788.7A CN111849929B (zh) | 2019-04-30 | 2019-04-30 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
| CN2019103647887 | 2019-04-30 | ||
| PCT/CN2020/088506 WO2020221365A1 (zh) | 2019-04-30 | 2020-04-30 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
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| CN202080032606.7A Pending CN113767168A (zh) | 2019-04-30 | 2020-04-30 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
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| CN111850020B (zh) * | 2019-04-25 | 2021-05-07 | 苏州鲲鹏生物技术有限公司 | 利用质粒系统在蛋白中引入非天然氨基酸 |
| CN111849929B (zh) * | 2019-04-30 | 2021-05-11 | 苏州鲲鹏生物技术有限公司 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
| CN115701451B (zh) * | 2021-08-02 | 2023-08-01 | 宁波鲲鹏生物科技有限公司 | 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用 |
| WO2023031445A2 (en) * | 2021-09-06 | 2023-03-09 | Veraxa Biotech Gmbh | Novel aminoacyl-trna synthetase variants for genetic code expansion in eukaryotes |
| CN114134141B (zh) * | 2021-11-03 | 2024-01-30 | 杭州嵌化合生医药科技有限公司 | 一种引入非天然氨基酸的嵌合体苯丙氨酸翻译系统及其构建方法 |
| CN114657156A (zh) * | 2022-03-11 | 2022-06-24 | 南京中医药大学 | 吡咯赖氨酸氨酰-tRNA合成酶突变体及其筛选系统和应用 |
| WO2023208081A1 (en) * | 2022-04-28 | 2023-11-02 | Shenzhen Bay Laboratory | Substituted fluorosulfate and use thereof |
| CN114908066B (zh) * | 2022-05-17 | 2024-01-23 | 杭州嵌化合生医药科技有限公司 | 一种正交翻译系统及其在再分配密码子恢复ptc疾病中功能蛋白表达方面的应用 |
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| JP5590649B2 (ja) * | 2007-09-20 | 2014-09-17 | 独立行政法人理化学研究所 | 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 |
| CN104099360A (zh) * | 2013-04-12 | 2014-10-15 | 北京大学 | 非天然氨基酸标记的目的蛋白或肽的制备 |
| EP2894476A1 (en) * | 2014-01-14 | 2015-07-15 | Universität Konstanz | Genetically Encoded Spin Label |
| GB201419109D0 (en) * | 2014-10-27 | 2014-12-10 | Medical Res Council | Incorporation of unnatural amino acids into proteins |
| CN107012121B (zh) * | 2016-01-27 | 2021-01-26 | 北京大学 | 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建 |
| CN107022568B (zh) * | 2016-02-01 | 2020-08-25 | 北京大学 | 哺乳动物细胞中高效多点插入非天然氨基酸的系统 |
| CN107177593B (zh) * | 2016-03-10 | 2020-10-23 | 北京大学 | 利用优化的基因密码子扩展系统通读提前终止密码子疾病中的截短蛋白 |
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| CN107099546B (zh) * | 2017-06-07 | 2019-11-19 | 胜武（北京）生物科技有限公司 | 一种调控嵌合抗原受体表达的方法 |
| CN111718920B (zh) * | 2019-03-19 | 2021-05-07 | 宁波鲲鹏生物科技有限公司 | 在蛋白中高效引入赖氨酸衍生物的氨酰基-tRNA合成酶 |
| CN111718949B (zh) * | 2019-03-19 | 2021-10-01 | 宁波鲲鹏生物科技有限公司 | 利用双质粒系统在蛋白中引入非天然氨基酸 |
| CN111850020B (zh) * | 2019-04-25 | 2021-05-07 | 苏州鲲鹏生物技术有限公司 | 利用质粒系统在蛋白中引入非天然氨基酸 |
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| WO2010139948A2 (en) * | 2009-06-04 | 2010-12-09 | Medical Research Council | Methods |
| CN104718285A (zh) * | 2012-05-18 | 2015-06-17 | 医药研究委员会 | 利用编码包含BCN基团的氨基酸的正交密码子和正交PylRS合成酶将包含BCN基团的氨基酸整合到多肽中的方法 |
| CN104450746A (zh) * | 2014-08-30 | 2015-03-25 | 武汉佰福泰制药有限公司 | 一种向蛋白质定点引入非天然氨基酸的方法 |
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| US20220290202A1 (en) | 2022-09-15 |
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| AU2020265979A1 (en) | 2021-12-23 |
| BR112021021859A2 (pt) | 2021-12-21 |
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| CA3138814A1 (en) | 2020-11-05 |
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