BR112021019657A2 - Método para produzir célula knock-in - Google Patents
Método para produzir célula knock-inInfo
- Publication number
- BR112021019657A2 BR112021019657A2 BR112021019657A BR112021019657A BR112021019657A2 BR 112021019657 A2 BR112021019657 A2 BR 112021019657A2 BR 112021019657 A BR112021019657 A BR 112021019657A BR 112021019657 A BR112021019657 A BR 112021019657A BR 112021019657 A2 BR112021019657 A2 BR 112021019657A2
- Authority
- BR
- Brazil
- Prior art keywords
- molecule
- genomic
- cleaves
- homology arm
- cell
- Prior art date
Links
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 230000006801 homologous recombination Effects 0.000 abstract 1
- 238000002744 homologous recombination Methods 0.000 abstract 1
- 230000006780 non-homologous end joining Effects 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
a presente invenção refere-se ao uso de um sistema de nuclease específico de sítio que inclui uma combinação de uma molécula que simultaneamente tem como alvo e cliva uma sequência do braço de homologia do dna doador e a sequência genômica correspondente à sequência do braço de homologia e uma molécula que tem como alvo e cliva a região genômica na vizinhança do sítio de clivagem por aquela molécula que causa o reparo entre o dna genômico e o dna doador por meio de junção de extremidade não homóloga e recombinação homóloga, tornando possível a produção de células e organismos com sequências doadoras longas knocked-in com alta eficiência e precisão.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2019072782 | 2019-04-05 | ||
PCT/JP2020/015291 WO2020204159A1 (ja) | 2019-04-05 | 2020-04-03 | ノックイン細胞の作製方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112021019657A2 true BR112021019657A2 (pt) | 2021-11-30 |
Family
ID=72668293
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112021019657A BR112021019657A2 (pt) | 2019-04-05 | 2020-04-03 | Método para produzir célula knock-in |
Country Status (9)
Country | Link |
---|---|
US (1) | US20220186263A1 (pt) |
EP (1) | EP3950942A4 (pt) |
JP (1) | JP7426120B2 (pt) |
KR (1) | KR20210148286A (pt) |
CN (1) | CN113646429A (pt) |
AU (1) | AU2020254078A1 (pt) |
BR (1) | BR112021019657A2 (pt) |
CA (1) | CA3136061A1 (pt) |
WO (1) | WO2020204159A1 (pt) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112746074B (zh) * | 2020-12-16 | 2022-12-02 | 南方医科大学皮肤病医院(广东省皮肤病医院、广东省皮肤性病防治中心、中国麻风防治研究中心) | Keratin 5-IRES-eGFP敲入的hESCs细胞系的构建及诱导分化方法 |
WO2024102954A1 (en) | 2022-11-10 | 2024-05-16 | Massachusetts Institute Of Technology | Activation induced clipping system (aics) |
Family Cites Families (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BRPI0307383B1 (pt) | 2002-01-23 | 2019-12-31 | The Univ Of Utah Research Foundation | método de recombinação genética direcionada em célula de planta hospedeira |
US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
CN102770539B (zh) | 2009-12-10 | 2016-08-03 | 明尼苏达大学董事会 | Tal效应子介导的dna修饰 |
GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
DK3401400T3 (da) | 2012-05-25 | 2019-06-03 | Univ California | Fremgangsmåder og sammensætninger til rna-styret mål-dna-modifikation og til rna-styret transskriptionsmodulering |
PT2896697E (pt) | 2012-12-12 | 2015-12-31 | Massachusetts Inst Technology | Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências |
KR20160007541A (ko) | 2013-04-22 | 2016-01-20 | 고쿠리쓰다이가쿠호진 규슈다이가쿠 | 피피알 모티프를 이용한 디앤에이 결합성 단백질 및 그의 이용 |
JP5931022B2 (ja) | 2013-08-09 | 2016-06-08 | 国立大学法人広島大学 | Dna結合ドメインを含むポリペプチド |
US10787684B2 (en) * | 2013-11-19 | 2020-09-29 | President And Fellows Of Harvard College | Large gene excision and insertion |
US10362771B2 (en) * | 2014-11-20 | 2019-07-30 | Kyoto University | Method for knock-in of DNA into target region of mammalian genome, and cell |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
US11905521B2 (en) * | 2015-11-17 | 2024-02-20 | The Chinese University Of Hong Kong | Methods and systems for targeted gene manipulation |
CN110214185A (zh) * | 2016-11-28 | 2019-09-06 | 国立大学法人大阪大学 | 基因组编辑方法 |
WO2018221685A1 (ja) | 2017-05-31 | 2018-12-06 | 国立大学法人 東京大学 | 改変されたCas9タンパク質及びその用途 |
KR102541398B1 (ko) | 2017-06-08 | 2023-06-07 | 고꾸리쯔 다이가꾸 호우징 오사까 다이가꾸 | Dna가 편집된 진핵 세포를 제조하는 방법 및 당해 방법에 사용되는 키트 |
BR112020003439A2 (pt) | 2017-08-21 | 2020-08-25 | Tokushima University | tecnologia de alteração específica de sequência alvo usando o reconhecimento alvo nucleotídico |
-
2020
- 2020-04-03 AU AU2020254078A patent/AU2020254078A1/en active Pending
- 2020-04-03 EP EP20783536.4A patent/EP3950942A4/en active Pending
- 2020-04-03 US US17/601,303 patent/US20220186263A1/en active Pending
- 2020-04-03 JP JP2021512319A patent/JP7426120B2/ja active Active
- 2020-04-03 WO PCT/JP2020/015291 patent/WO2020204159A1/ja unknown
- 2020-04-03 CA CA3136061A patent/CA3136061A1/en active Pending
- 2020-04-03 KR KR1020217035792A patent/KR20210148286A/ko unknown
- 2020-04-03 BR BR112021019657A patent/BR112021019657A2/pt unknown
- 2020-04-03 CN CN202080025993.1A patent/CN113646429A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
US20220186263A1 (en) | 2022-06-16 |
JP7426120B2 (ja) | 2024-02-01 |
EP3950942A1 (en) | 2022-02-09 |
AU2020254078A1 (en) | 2021-12-02 |
JPWO2020204159A1 (pt) | 2020-10-08 |
EP3950942A4 (en) | 2023-01-18 |
WO2020204159A1 (ja) | 2020-10-08 |
CA3136061A1 (en) | 2020-10-08 |
CN113646429A (zh) | 2021-11-12 |
KR20210148286A (ko) | 2021-12-07 |
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