AR083221A1 - Proceso para producir dialquilfosfotriesteres de nucleosidos mediante transesterificacion enzimatica y desproteccion de los mismos para producir nucleosidos monofosfato - Google Patents
Proceso para producir dialquilfosfotriesteres de nucleosidos mediante transesterificacion enzimatica y desproteccion de los mismos para producir nucleosidos monofosfatoInfo
- Publication number
- AR083221A1 AR083221A1 ARP110103609A ARP110103609A AR083221A1 AR 083221 A1 AR083221 A1 AR 083221A1 AR P110103609 A ARP110103609 A AR P110103609A AR P110103609 A ARP110103609 A AR P110103609A AR 083221 A1 AR083221 A1 AR 083221A1
- Authority
- AR
- Argentina
- Prior art keywords
- nucleoside
- phosphotriester
- produce
- dialkyl
- monophosphate
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 230000002255 enzymatic effect Effects 0.000 title abstract 2
- 238000005809 transesterification reaction Methods 0.000 title abstract 2
- VZQXUWKZDSEQRR-UHFFFAOYSA-N Nucleosid Natural products C12=NC(SC)=NC(NCC=C(C)C)=C2N=CN1C1OC(CO)C(O)C1O VZQXUWKZDSEQRR-UHFFFAOYSA-N 0.000 title 1
- 238000010511 deprotection reaction Methods 0.000 title 1
- 150000004712 monophosphates Chemical class 0.000 title 1
- 239000002777 nucleoside Substances 0.000 abstract 9
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract 6
- 102000004203 Phosphoric Triester Hydrolases Human genes 0.000 abstract 3
- 108090000754 Phosphoric Triester Hydrolases Proteins 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- -1 monophosphate nucleoside Chemical class 0.000 abstract 3
- 229910019142 PO4 Inorganic materials 0.000 abstract 2
- 125000000217 alkyl group Chemical group 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract 2
- 239000010452 phosphate Substances 0.000 abstract 2
- 102000004861 Phosphoric Diester Hydrolases Human genes 0.000 abstract 1
- 108090001050 Phosphoric Diester Hydrolases Proteins 0.000 abstract 1
- 239000013043 chemical agent Substances 0.000 abstract 1
- 238000005984 hydrogenation reaction Methods 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 150000007522 mineralic acids Chemical class 0.000 abstract 1
- 150000007524 organic acids Chemical class 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/04—Phosphoric diester hydrolases (3.1.4)
- C12Y301/04001—Phosphodiesterase I (3.1.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/08—Phosphoric triester hydrolases (3.1.8)
- C12Y301/08001—Aryldialkylphosphatase (3.1.8.1), i.e. paraoxonase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Reivindicación 1: Un proceso para producir un dialquilfosfotriéster de nucleósido, donde el proceso comprende la reacción de un nucleósido con un compuesto fosfotriéster activado como un dador de fosfato en presencia de una enzima fosfotriesterasa.Reivindicación 12: Un proceso para producir un nucleósido monofosfato monoéster, el proceso que comprende las etapas de: a) conducir la transesterificación enzimática de acuerdo con la reivindicación 1, mediante la reacción de un nucleósido con un compuesto fosfotriéster activado como un dador de fosfato en presencia de una enzima fosfotriesterasa, b) eliminar los grupos alquilo del dialquilfosfotriéster de nucleósido obtenido en la etapa (a) mediante reacción con un agente químico seleccionado del grupo de reactivos básicos inorgánicos u orgánicos, reactivos ácidos inorgánicos u orgánicos, tioalcoholes y reactivos de hidrogenación, o c) eliminar los grupos alquilo del dialquilfosfotriéster de nucleósido obtenido en la etapa (a) mediante hidrólisis de acuerdo con las siguientes etapas: i) incubar el dialquilfosfotriéster de nucleósido en presencia de una enzima fosfotriesterasa, ii) incubar el alquilfosfodiéster de nucleósido de la etapa (i) en presencia de una enzima fosfodiesterasa, y recoger el nucleósido monofosfato de la mezcla de reacción.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38767010P | 2010-09-29 | 2010-09-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR083221A1 true AR083221A1 (es) | 2013-02-06 |
Family
ID=44773066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP110103609A AR083221A1 (es) | 2010-09-29 | 2011-09-29 | Proceso para producir dialquilfosfotriesteres de nucleosidos mediante transesterificacion enzimatica y desproteccion de los mismos para producir nucleosidos monofosfato |
Country Status (2)
| Country | Link |
|---|---|
| AR (1) | AR083221A1 (es) |
| WO (1) | WO2012041965A1 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG10201610936RA (en) | 2011-12-22 | 2017-02-27 | Alios Biopharma Inc | Substituted nucleosides, nucleotides and analogs thereof |
| US9441007B2 (en) | 2012-03-21 | 2016-09-13 | Alios Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
| USRE48171E1 (en) | 2012-03-21 | 2020-08-25 | Janssen Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
| WO2013174962A1 (en) | 2012-05-25 | 2013-11-28 | Janssen R&D Ireland | Uracyl spirooxetane nucleosides |
| CN110684067A (zh) | 2012-12-21 | 2020-01-14 | 艾丽奥斯生物制药有限公司 | 取代的核苷和核苷酸 |
| WO2014169278A1 (en) | 2013-04-12 | 2014-10-16 | Achillion Pharmaceuticals, Inc. | Highly active nucleoside derivative for the treatment of hcv |
| AU2014331863C1 (en) | 2013-10-11 | 2019-05-16 | Alios Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
| GEAP202215528A (en) | 2015-03-06 | 2022-11-10 | Atea Pharmaceuticals Inc | B-D-2'-DEOXY-2'a-FLUORO-2'-B-C-SUBSTITUTED-2-MODIFIED-N6-SUBSTITUTED PURINE NUCLEOTIDES FOR HCV TREATMENT |
| KR102630013B1 (ko) | 2015-08-06 | 2024-01-25 | 키메릭스 인크. | 항바이러스제로서 유용한 피롤로피리미딘 뉴클레오시드 및 그의 유사체 |
| BR112019004297A2 (pt) | 2016-09-07 | 2019-05-28 | Atea Pharmaceuticals Inc | método, uso de uma quantidade eficaz de um composto, composto, e, composição farmacêutica. |
| SG11201906163TA (en) | 2017-02-01 | 2019-08-27 | Atea Pharmaceuticals Inc | Nucleotide hemi-sulfate salt for the treatment of hepatitis c virus |
| US11111264B2 (en) | 2017-09-21 | 2021-09-07 | Chimerix, Inc. | Morphic forms of 4-amino-7-(3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-2-methyl-7H-pyrrolo[2,3-d]pyrimidine-5-carboxamide and uses thereof |
| EP3773753A4 (en) | 2018-04-10 | 2021-12-22 | ATEA Pharmaceuticals, Inc. | TREATMENT OF PATIENTS INFECTED WITH THE HEPATITIS C VIRUS WITH CIRRHOSIS |
| US10874687B1 (en) | 2020-02-27 | 2020-12-29 | Atea Pharmaceuticals, Inc. | Highly active compounds against COVID-19 |
| JP2024525164A (ja) | 2021-06-17 | 2024-07-10 | アテア ファーマシューティカルズ， インコーポレイテッド | 有利な抗ｈｃｖ併用療法 |
| JP2025509635A (ja) | 2022-03-15 | 2025-04-11 | ローム・セラピューティクス・インコーポレイテッド | 疾患を治療するための化合物及び方法 |
| WO2024086592A2 (en) * | 2022-10-17 | 2024-04-25 | Emory Unversity | 4'-halogen containing nucleotide and nucleoside therapeutic compositions and uses related thereto |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5756315A (en) | 1989-12-05 | 1998-05-26 | Kyowa Hakko Kogyo Co., Ltd. | Inosine-guanosine kinase |
| KR20010085993A (ko) | 1999-09-03 | 2001-09-07 | 에가시라 구니오 | 돌연변이형 뉴클레오시드-5'-포스페이트 생성 효소 |
| AUPR502301A0 (en) * | 2001-05-15 | 2001-06-07 | Commonwealth Scientific And Industrial Research Organisation | Phosphotriesterase from Agrobacterium radiobacter P230 |
-
2011
- 2011-09-29 AR ARP110103609A patent/AR083221A1/es unknown
- 2011-09-29 WO PCT/EP2011/066988 patent/WO2012041965A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012041965A1 (en) | 2012-04-05 |
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