BR112021001978A2 - molécula de ácido ribonucleico quimérico, polinucleotídeo isolado e/ou exógeno, vetor, célula hospedeira, organismo não humano, método para produzir uma molécula de ácido ribonucleico quimérico, extrato de uma célula hospedeira, composição, método para reduzir ou infrarregular o nível e/ou atividade de uma molécula de rna alvo em uma célula ou um organismo, método para reduzir danos causados por uma praga ou patógeno a um organismo não humano, método para controlar um organismo não humano, método para evitar ou tratar uma doença em um sujeito, uso de uma molécula de ácido ribonucleico quimérico e kit - Google Patents

molécula de ácido ribonucleico quimérico, polinucleotídeo isolado e/ou exógeno, vetor, célula hospedeira, organismo não humano, método para produzir uma molécula de ácido ribonucleico quimérico, extrato de uma célula hospedeira, composição, método para reduzir ou infrarregular o nível e/ou atividade de uma molécula de rna alvo em uma célula ou um organismo, método para reduzir danos causados por uma praga ou patógeno a um organismo não humano, método para controlar um organismo não humano, método para evitar ou tratar uma doença em um sujeito, uso de uma molécula de ácido ribonucleico quimérico e kit Download PDF

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Publication number
BR112021001978A2
BR112021001978A2 BR112021001978-2A BR112021001978A BR112021001978A2 BR 112021001978 A2 BR112021001978 A2 BR 112021001978A2 BR 112021001978 A BR112021001978 A BR 112021001978A BR 112021001978 A2 BR112021001978 A2 BR 112021001978A2
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BR
Brazil
Prior art keywords
sequence
rna
ribonucleotide
molecule
antisense
Prior art date
Application number
BR112021001978-2A
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English (en)
Portuguese (pt)
Inventor
Neil Andrew SMITH
Robert De Feyter
Ming Bo WANG
Daai ZHANG
Timothy James Doran
Mark Tizard
Annapurna Devi ALLU
Ian Kevin GREAVES
Lingling Gao
Jonathan Paul ANDERSON
Original Assignee
Commonwealth Scientific And Industrial Research Organisation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2018902840A external-priority patent/AU2018902840A0/en
Priority claimed from PCT/AU2018/051015 external-priority patent/WO2019051563A1/en
Application filed by Commonwealth Scientific And Industrial Research Organisation filed Critical Commonwealth Scientific And Industrial Research Organisation
Publication of BR112021001978A2 publication Critical patent/BR112021001978A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
  • Catching Or Destruction (AREA)
BR112021001978-2A 2018-08-03 2019-08-02 molécula de ácido ribonucleico quimérico, polinucleotídeo isolado e/ou exógeno, vetor, célula hospedeira, organismo não humano, método para produzir uma molécula de ácido ribonucleico quimérico, extrato de uma célula hospedeira, composição, método para reduzir ou infrarregular o nível e/ou atividade de uma molécula de rna alvo em uma célula ou um organismo, método para reduzir danos causados por uma praga ou patógeno a um organismo não humano, método para controlar um organismo não humano, método para evitar ou tratar uma doença em um sujeito, uso de uma molécula de ácido ribonucleico quimérico e kit BR112021001978A2 (pt)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
AU2018902840A AU2018902840A0 (en) 2018-08-03 RNA molecules
AU2018902840 2018-08-03
AU2018902896 2018-08-08
AU2018902896A AU2018902896A0 (en) 2018-08-08 RNA molecules
PCT/AU2018/051015 WO2019051563A1 (en) 2017-09-15 2018-09-17 Rna molecules
AUPCT/AU2018/051015 2018-09-17
AU2019900941 2019-03-20
AU2019900941A AU2019900941A0 (en) 2019-03-20 RNA molecules
PCT/AU2019/050814 WO2020024019A1 (en) 2018-08-03 2019-08-02 Rna molecules comprising non-canonical base pairs

Publications (1)

Publication Number Publication Date
BR112021001978A2 true BR112021001978A2 (pt) 2021-05-04

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ID=69230474

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021001978-2A BR112021001978A2 (pt) 2018-08-03 2019-08-02 molécula de ácido ribonucleico quimérico, polinucleotídeo isolado e/ou exógeno, vetor, célula hospedeira, organismo não humano, método para produzir uma molécula de ácido ribonucleico quimérico, extrato de uma célula hospedeira, composição, método para reduzir ou infrarregular o nível e/ou atividade de uma molécula de rna alvo em uma célula ou um organismo, método para reduzir danos causados por uma praga ou patógeno a um organismo não humano, método para controlar um organismo não humano, método para evitar ou tratar uma doença em um sujeito, uso de uma molécula de ácido ribonucleico quimérico e kit

Country Status (16)

Country Link
US (1) US20210269800A1 (https=)
EP (1) EP3830268A4 (https=)
JP (2) JP2021533812A (https=)
KR (2) KR20260036292A (https=)
CN (1) CN112888785A (https=)
AU (2) AU2019313162B2 (https=)
BR (1) BR112021001978A2 (https=)
CA (1) CA3108536A1 (https=)
IL (1) IL280562A (https=)
MX (1) MX2021001360A (https=)
MY (1) MY210286A (https=)
PH (1) PH12021500009A1 (https=)
SG (1) SG11202101059WA (https=)
UA (1) UA129911C2 (https=)
WO (1) WO2020024019A1 (https=)
ZA (1) ZA202101256B (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4007813A4 (en) * 2019-08-02 2023-08-30 Commonwealth Scientific and Industrial Research Organisation Rna molecules for modulating flowering in plants
EP4110918A4 (en) * 2020-02-28 2024-04-03 The University of Chicago METHODS AND COMPOSITIONS COMPRISING TRANS-ACTING TRANSLATION ACTIVATORS
WO2022011452A1 (en) * 2020-07-15 2022-01-20 University Of Manitoba Use of paperclip rna structure to inhibit target gene expression
CN111979210A (zh) * 2020-08-31 2020-11-24 中国农业大学 辣椒疫霉纤维素合酶蛋白及其编码基因与应用
EP4519445A2 (en) * 2022-05-04 2025-03-12 University of Maryland, College Park Rna vectors with hairpin-like inserts
CN115011609B (zh) * 2022-06-06 2024-03-19 江苏大学 BnaFANCM基因、应用及获得油菜雄性不育突变体的方法
CN115029349B (zh) * 2022-06-09 2024-11-12 中国农业大学 与植物抗病性相关的circRNA、来源基因及其应用
CN116478262B (zh) * 2022-09-28 2024-03-22 西南大学 RACK1在抗家蚕核型多角体病毒BmNPV中的应用
WO2024164008A1 (en) * 2023-02-03 2024-08-08 Sirnaomics, Inc. Products and compositions
WO2026000033A1 (en) * 2024-06-28 2026-01-02 Commonwealth Scientific And Industrial Research Organisation Double stranded rna molecules with a to g substitutions

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
CN101370940A (zh) * 2006-01-12 2009-02-18 德福根有限公司 作为昆虫防治剂的dsRNA
AU2008211580B2 (en) * 2007-01-29 2013-08-29 University Of The Witwatersrand Johannesburg Use of double stranded RNA hairpin duplexes in gene silencing
US8841437B2 (en) * 2008-06-20 2014-09-23 The Board Of Trustees Of The Leland Stanford Junior University Precursor miRNA loop-modulated target regulation
GB0810912D0 (en) * 2008-06-13 2008-07-23 Inst Animal Health Ltd Vector
WO2011109380A1 (en) * 2010-03-01 2011-09-09 The Children's Hospital Of Philadelphia Nucleic acids for targeting multiple regions of the hcv genome
US20130245102A1 (en) * 2010-10-12 2013-09-19 Research Foundation Of The City University Of New York Novel dna templates for small rna production in mammalian cells
CN102352360B (zh) * 2011-11-11 2012-10-10 南开大学 抑制hiv-1的双长发卡结构rna表达原件的构建及功能检测
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

Also Published As

Publication number Publication date
KR20210054518A (ko) 2021-05-13
US20210269800A1 (en) 2021-09-02
UA129911C2 (uk) 2025-09-10
CA3108536A1 (en) 2020-02-06
AU2019313162B2 (en) 2024-02-22
IL280562A (en) 2021-03-25
AU2019313162A1 (en) 2021-03-18
PH12021500009A1 (en) 2021-09-13
EP3830268A1 (en) 2021-06-09
KR20260036292A (ko) 2026-03-16
WO2020024019A1 (en) 2020-02-06
ZA202101256B (en) 2022-09-28
SG11202101059WA (en) 2021-02-25
AU2024203407A1 (en) 2024-08-01
MX2021001360A (es) 2021-07-16
JP2021533812A (ja) 2021-12-09
MY210286A (en) 2025-09-08
CN112888785A (zh) 2021-06-01
EP3830268A4 (en) 2023-12-13
JP2024133533A (ja) 2024-10-02

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