AU2019313162B2 - RNA molecules comprising non-canonical base pairs - Google Patents

RNA molecules comprising non-canonical base pairs Download PDF

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Publication number
AU2019313162B2
AU2019313162B2 AU2019313162A AU2019313162A AU2019313162B2 AU 2019313162 B2 AU2019313162 B2 AU 2019313162B2 AU 2019313162 A AU2019313162 A AU 2019313162A AU 2019313162 A AU2019313162 A AU 2019313162A AU 2019313162 B2 AU2019313162 B2 AU 2019313162B2
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sequence
ribonucleotide
rna molecule
antisense
sense
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AU2019313162A1 (en
Inventor
Annapurna Devi ALLU
Jonathan Paul ANDERSON
Robert DE FEYTER
Timothy James Doran
Lingling Gao
Ian Kevin GREAVES
Neil Andrew Smith
Mark Tizard
Ming Bo Wang
Daai ZHANG
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Priority claimed from PCT/AU2018/051015 external-priority patent/WO2019051563A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands

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  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
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AU2019313162A 2018-08-03 2019-08-02 RNA molecules comprising non-canonical base pairs Active AU2019313162B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2024203407A AU2024203407A1 (en) 2018-08-03 2024-05-22 RNA molecules comprising non-canonical base pairs

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
AU2018902840A AU2018902840A0 (en) 2018-08-03 RNA molecules
AU2018902840 2018-08-03
AU2018902896 2018-08-08
AU2018902896A AU2018902896A0 (en) 2018-08-08 RNA molecules
PCT/AU2018/051015 WO2019051563A1 (en) 2017-09-15 2018-09-17 Rna molecules
AUPCT/AU2018/051015 2018-09-17
AU2019900941 2019-03-20
AU2019900941A AU2019900941A0 (en) 2019-03-20 RNA molecules
PCT/AU2019/050814 WO2020024019A1 (en) 2018-08-03 2019-08-02 Rna molecules comprising non-canonical base pairs

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AU2019313162A1 AU2019313162A1 (en) 2021-03-18
AU2019313162B2 true AU2019313162B2 (en) 2024-02-22

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US (1) US20210269800A1 (https=)
EP (1) EP3830268A4 (https=)
JP (2) JP2021533812A (https=)
KR (2) KR20260036292A (https=)
CN (1) CN112888785A (https=)
AU (2) AU2019313162B2 (https=)
BR (1) BR112021001978A2 (https=)
CA (1) CA3108536A1 (https=)
IL (1) IL280562A (https=)
MX (1) MX2021001360A (https=)
MY (1) MY210286A (https=)
PH (1) PH12021500009A1 (https=)
SG (1) SG11202101059WA (https=)
UA (1) UA129911C2 (https=)
WO (1) WO2020024019A1 (https=)
ZA (1) ZA202101256B (https=)

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EP4007813A4 (en) * 2019-08-02 2023-08-30 Commonwealth Scientific and Industrial Research Organisation Rna molecules for modulating flowering in plants
EP4110918A4 (en) * 2020-02-28 2024-04-03 The University of Chicago METHODS AND COMPOSITIONS COMPRISING TRANS-ACTING TRANSLATION ACTIVATORS
WO2022011452A1 (en) * 2020-07-15 2022-01-20 University Of Manitoba Use of paperclip rna structure to inhibit target gene expression
CN111979210A (zh) * 2020-08-31 2020-11-24 中国农业大学 辣椒疫霉纤维素合酶蛋白及其编码基因与应用
EP4519445A2 (en) * 2022-05-04 2025-03-12 University of Maryland, College Park Rna vectors with hairpin-like inserts
CN115011609B (zh) * 2022-06-06 2024-03-19 江苏大学 BnaFANCM基因、应用及获得油菜雄性不育突变体的方法
CN115029349B (zh) * 2022-06-09 2024-11-12 中国农业大学 与植物抗病性相关的circRNA、来源基因及其应用
CN116478262B (zh) * 2022-09-28 2024-03-22 西南大学 RACK1在抗家蚕核型多角体病毒BmNPV中的应用
WO2024164008A1 (en) * 2023-02-03 2024-08-08 Sirnaomics, Inc. Products and compositions
WO2026000033A1 (en) * 2024-06-28 2026-01-02 Commonwealth Scientific And Industrial Research Organisation Double stranded rna molecules with a to g substitutions

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WO2009150431A1 (en) * 2008-06-13 2009-12-17 Institute For Animal Health Vector
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KR20210054518A (ko) 2021-05-13
US20210269800A1 (en) 2021-09-02
UA129911C2 (uk) 2025-09-10
CA3108536A1 (en) 2020-02-06
IL280562A (en) 2021-03-25
AU2019313162A1 (en) 2021-03-18
PH12021500009A1 (en) 2021-09-13
BR112021001978A2 (pt) 2021-05-04
EP3830268A1 (en) 2021-06-09
KR20260036292A (ko) 2026-03-16
WO2020024019A1 (en) 2020-02-06
ZA202101256B (en) 2022-09-28
SG11202101059WA (en) 2021-02-25
AU2024203407A1 (en) 2024-08-01
MX2021001360A (es) 2021-07-16
JP2021533812A (ja) 2021-12-09
MY210286A (en) 2025-09-08
CN112888785A (zh) 2021-06-01
EP3830268A4 (en) 2023-12-13
JP2024133533A (ja) 2024-10-02

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