CN112888785A - 包含非规范碱基对的rna分子 - Google Patents

包含非规范碱基对的rna分子 Download PDF

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Publication number
CN112888785A
CN112888785A CN201980065254.2A CN201980065254A CN112888785A CN 112888785 A CN112888785 A CN 112888785A CN 201980065254 A CN201980065254 A CN 201980065254A CN 112888785 A CN112888785 A CN 112888785A
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Prior art keywords
sequence
rna molecule
ribonucleotide
base pairs
antisense
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CN201980065254.2A
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English (en)
Chinese (zh)
Inventor
N·A·史密斯
M·B·王
D·张
T·J·多兰
M·蒂泽德
A·D·阿鲁
I·K·格里夫斯
L·高
J·P·安德森
R·德菲特
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Commonwealth Scientific and Industrial Research Organization CSIRO
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Priority claimed from AU2018902840A external-priority patent/AU2018902840A0/en
Priority claimed from PCT/AU2018/051015 external-priority patent/WO2019051563A1/en
Application filed by Commonwealth Scientific and Industrial Research Organization CSIRO filed Critical Commonwealth Scientific and Industrial Research Organization CSIRO
Publication of CN112888785A publication Critical patent/CN112888785A/zh
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/33—Chemical structure of the base
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
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    • C12N2310/532—Closed or circular
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    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands

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  • Cell Biology (AREA)
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  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN201980065254.2A 2018-08-03 2019-08-02 包含非规范碱基对的rna分子 Pending CN112888785A (zh)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
AU2018902840A AU2018902840A0 (en) 2018-08-03 RNA molecules
AU2018902840 2018-08-03
AU2018902896A AU2018902896A0 (en) 2018-08-08 RNA molecules
AU2018902896 2018-08-08
AUPCT/AU2018/051015 2018-09-17
PCT/AU2018/051015 WO2019051563A1 (en) 2017-09-15 2018-09-17 Rna molecules
AU2019900941 2019-03-20
AU2019900941A AU2019900941A0 (en) 2019-03-20 RNA molecules
PCT/AU2019/050814 WO2020024019A1 (en) 2018-08-03 2019-08-02 Rna molecules comprising non-canonical base pairs

Publications (1)

Publication Number Publication Date
CN112888785A true CN112888785A (zh) 2021-06-01

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CN201980065254.2A Pending CN112888785A (zh) 2018-08-03 2019-08-02 包含非规范碱基对的rna分子

Country Status (16)

Country Link
US (1) US20210269800A1 (https=)
EP (1) EP3830268A4 (https=)
JP (2) JP2021533812A (https=)
KR (2) KR20210054518A (https=)
CN (1) CN112888785A (https=)
AU (2) AU2019313162B2 (https=)
BR (1) BR112021001978A2 (https=)
CA (1) CA3108536A1 (https=)
IL (1) IL280562A (https=)
MX (1) MX2021001360A (https=)
MY (1) MY210286A (https=)
PH (1) PH12021500009A1 (https=)
SG (1) SG11202101059WA (https=)
UA (1) UA129911C2 (https=)
WO (1) WO2020024019A1 (https=)
ZA (1) ZA202101256B (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115011609A (zh) * 2022-06-06 2022-09-06 江苏大学 BnaFANCM基因、应用及获得油菜雄性不育突变体的方法
CN115029349A (zh) * 2022-06-09 2022-09-09 中国农业大学 与植物抗病性相关的circRNA、来源基因及其应用
CN116478262A (zh) * 2022-09-28 2023-07-25 西南大学 RACK1在抗家蚕核型多角体病毒BmNPV中的应用

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022001439A (es) * 2019-08-02 2022-05-06 Commw Scient Ind Res Org Moléculas de arn para modular el florecimiento en plantas.
US20230090706A1 (en) * 2020-02-28 2023-03-23 The University Of Chicago Methods and compositions comprising trans-acting translational activators
CA3185762A1 (en) * 2020-07-15 2022-01-20 Steve Whyard Use of paperclip rna structure to inhibit target gene expression
CN111979210A (zh) * 2020-08-31 2020-11-24 中国农业大学 辣椒疫霉纤维素合酶蛋白及其编码基因与应用
WO2023215782A2 (en) * 2022-05-04 2023-11-09 University Of Maryland, College Park Rna vectors with hairpin-like inserts
WO2024164008A1 (en) * 2023-02-03 2024-08-08 Sirnaomics, Inc. Products and compositions
WO2026000033A1 (en) * 2024-06-28 2026-01-02 Commonwealth Scientific And Industrial Research Organisation Double stranded rna molecules with a to g substitutions

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US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
CN101370940A (zh) * 2006-01-12 2009-02-18 德福根有限公司 作为昆虫防治剂的dsRNA
WO2009150431A1 (en) * 2008-06-13 2009-12-17 Institute For Animal Health Vector
CN102352360A (zh) * 2011-11-11 2012-02-15 南开大学 抑制hiv-1的双长发卡结构rna表达原件的构建及功能检测
WO2012051152A2 (en) * 2010-10-12 2012-04-19 Kevin Ryan Novel dna templates for small rna production in mammalian cells
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

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AU2008211580B2 (en) * 2007-01-29 2013-08-29 University Of The Witwatersrand Johannesburg Use of double stranded RNA hairpin duplexes in gene silencing
US8841437B2 (en) * 2008-06-20 2014-09-23 The Board Of Trustees Of The Leland Stanford Junior University Precursor miRNA loop-modulated target regulation
EP2542247A4 (en) * 2010-03-01 2014-05-07 Philadelphia Children Hospital NUCLEIC ACID FOR ADDRESSING MULTIPLE REGIONS OF THE HCV GENOM

Patent Citations (7)

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Publication number Priority date Publication date Assignee Title
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
CN101370940A (zh) * 2006-01-12 2009-02-18 德福根有限公司 作为昆虫防治剂的dsRNA
WO2009150431A1 (en) * 2008-06-13 2009-12-17 Institute For Animal Health Vector
US20120076823A1 (en) * 2008-06-13 2012-03-29 Institute For Animal Health Vector
WO2012051152A2 (en) * 2010-10-12 2012-04-19 Kevin Ryan Novel dna templates for small rna production in mammalian cells
CN102352360A (zh) * 2011-11-11 2012-02-15 南开大学 抑制hiv-1的双长发卡结构rna表达原件的构建及功能检测
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

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Title
CHANG LIU 等: "Efficacy Analysis of Combinatorial siRNAs against HIV Derived from One Double Hairpin RNA Precursor", FRONT MICROBIOL., vol. 8, pages 2 *
HERRERA-CARRILLO等: "Probing the shRNA characteristics that hinder Dicer recognition and consequently allow Ago-mediated processing and AgoshRNA activity", RNA, vol. 20, pages 1410, XP055488337, DOI: 10.1261/rna.043950.113 *
MASAYUKI SANO等: "Expression of long anti-HIV-1 Hairpin RNAs for the Generation of Multiple siRNAs：advantages and limitations", MOLECULAR THERAPY, vol. 16, no. 1, pages 1, XP093052077, DOI: 10.1038/sj.mt.6300298 *
TARRYN BOURHILL等: "Successful disabling of the 5’UTR of HCV using adeno-associated viral vectors modular multimeric primary microRNA mimics", JOURNAL OF VIROLOGICAL METHODS, vol. 235, pages 26 - 33, XP029689268, DOI: 10.1016/j.jviromet.2016.05.008 *
梁之聘: "多靶位RNA干扰对HIV-1抑制作用的研究", 中国博士学位论文全文数据库（电子期刊）医药卫生科技, no. 4, pages 061 - 23 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115011609A (zh) * 2022-06-06 2022-09-06 江苏大学 BnaFANCM基因、应用及获得油菜雄性不育突变体的方法
CN115011609B (zh) * 2022-06-06 2024-03-19 江苏大学 BnaFANCM基因、应用及获得油菜雄性不育突变体的方法
CN115029349A (zh) * 2022-06-09 2022-09-09 中国农业大学 与植物抗病性相关的circRNA、来源基因及其应用
CN116478262A (zh) * 2022-09-28 2023-07-25 西南大学 RACK1在抗家蚕核型多角体病毒BmNPV中的应用
CN116478262B (zh) * 2022-09-28 2024-03-22 西南大学 RACK1在抗家蚕核型多角体病毒BmNPV中的应用

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PH12021500009A1 (en) 2021-09-13
UA129911C2 (uk) 2025-09-10
CA3108536A1 (en) 2020-02-06
KR20260036292A (ko) 2026-03-16
JP2024133533A (ja) 2024-10-02
AU2019313162A1 (en) 2021-03-18
KR20210054518A (ko) 2021-05-13
EP3830268A1 (en) 2021-06-09
ZA202101256B (en) 2022-09-28
MX2021001360A (es) 2021-07-16
US20210269800A1 (en) 2021-09-02
SG11202101059WA (en) 2021-02-25
AU2024203407A1 (en) 2024-08-01
IL280562A (en) 2021-03-25
BR112021001978A2 (pt) 2021-05-04
AU2019313162B2 (en) 2024-02-22
EP3830268A4 (en) 2023-12-13
WO2020024019A1 (en) 2020-02-06
MY210286A (en) 2025-09-08
JP2021533812A (ja) 2021-12-09

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