ZA202101256B - Rna molecules comprising non-canonical base pairs - Google Patents

Rna molecules comprising non-canonical base pairs

Info

Publication number
ZA202101256B
ZA202101256B ZA2021/01256A ZA202101256A ZA202101256B ZA 202101256 B ZA202101256 B ZA 202101256B ZA 2021/01256 A ZA2021/01256 A ZA 2021/01256A ZA 202101256 A ZA202101256 A ZA 202101256A ZA 202101256 B ZA202101256 B ZA 202101256B
Authority
ZA
South Africa
Prior art keywords
base pairs
rna molecules
canonical base
canonical
rna
Prior art date
Application number
ZA2021/01256A
Other languages
English (en)
Inventor
Andrew Smith Neil
Bo Wang Ming
Zhang Daai
James Doran Timothy
Tizard Mark
Devi Allu Annapurna
Kevin Greaves Ian
Gao Lingling
Paul Anderson Jonathan
De Feyter Robert
Original Assignee
Commw Scient Ind Res Org
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2018902840A external-priority patent/AU2018902840A0/en
Priority claimed from PCT/AU2018/051015 external-priority patent/WO2019051563A1/en
Application filed by Commw Scient Ind Res Org filed Critical Commw Scient Ind Res Org
Publication of ZA202101256B publication Critical patent/ZA202101256B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Catching Or Destruction (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
ZA2021/01256A 2018-08-03 2021-02-24 Rna molecules comprising non-canonical base pairs ZA202101256B (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
AU2018902840A AU2018902840A0 (en) 2018-08-03 RNA molecules
AU2018902896A AU2018902896A0 (en) 2018-08-08 RNA molecules
PCT/AU2018/051015 WO2019051563A1 (en) 2017-09-15 2018-09-17 Rna molecules
AU2019900941A AU2019900941A0 (en) 2019-03-20 RNA molecules
PCT/AU2019/050814 WO2020024019A1 (en) 2018-08-03 2019-08-02 Rna molecules comprising non-canonical base pairs

Publications (1)

Publication Number Publication Date
ZA202101256B true ZA202101256B (en) 2022-09-28

Family

ID=69230474

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2021/01256A ZA202101256B (en) 2018-08-03 2021-02-24 Rna molecules comprising non-canonical base pairs

Country Status (16)

Country Link
US (1) US20210269800A1 (https=)
EP (1) EP3830268A4 (https=)
JP (2) JP2021533812A (https=)
KR (2) KR20210054518A (https=)
CN (1) CN112888785A (https=)
AU (2) AU2019313162B2 (https=)
BR (1) BR112021001978A2 (https=)
CA (1) CA3108536A1 (https=)
IL (1) IL280562A (https=)
MX (1) MX2021001360A (https=)
MY (1) MY210286A (https=)
PH (1) PH12021500009A1 (https=)
SG (1) SG11202101059WA (https=)
UA (1) UA129911C2 (https=)
WO (1) WO2020024019A1 (https=)
ZA (1) ZA202101256B (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022001439A (es) * 2019-08-02 2022-05-06 Commw Scient Ind Res Org Moléculas de arn para modular el florecimiento en plantas.
US20230090706A1 (en) * 2020-02-28 2023-03-23 The University Of Chicago Methods and compositions comprising trans-acting translational activators
CA3185762A1 (en) * 2020-07-15 2022-01-20 Steve Whyard Use of paperclip rna structure to inhibit target gene expression
CN111979210A (zh) * 2020-08-31 2020-11-24 中国农业大学 辣椒疫霉纤维素合酶蛋白及其编码基因与应用
WO2023215782A2 (en) * 2022-05-04 2023-11-09 University Of Maryland, College Park Rna vectors with hairpin-like inserts
CN115011609B (zh) * 2022-06-06 2024-03-19 江苏大学 BnaFANCM基因、应用及获得油菜雄性不育突变体的方法
CN115029349B (zh) * 2022-06-09 2024-11-12 中国农业大学 与植物抗病性相关的circRNA、来源基因及其应用
CN116478262B (zh) * 2022-09-28 2024-03-22 西南大学 RACK1在抗家蚕核型多角体病毒BmNPV中的应用
WO2024164008A1 (en) * 2023-02-03 2024-08-08 Sirnaomics, Inc. Products and compositions
WO2026000033A1 (en) * 2024-06-28 2026-01-02 Commonwealth Scientific And Industrial Research Organisation Double stranded rna molecules with a to g substitutions

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
CN101370940A (zh) * 2006-01-12 2009-02-18 德福根有限公司 作为昆虫防治剂的dsRNA
AU2008211580B2 (en) * 2007-01-29 2013-08-29 University Of The Witwatersrand Johannesburg Use of double stranded RNA hairpin duplexes in gene silencing
US8841437B2 (en) * 2008-06-20 2014-09-23 The Board Of Trustees Of The Leland Stanford Junior University Precursor miRNA loop-modulated target regulation
GB0810912D0 (en) * 2008-06-13 2008-07-23 Inst Animal Health Ltd Vector
EP2542247A4 (en) * 2010-03-01 2014-05-07 Philadelphia Children Hospital NUCLEIC ACID FOR ADDRESSING MULTIPLE REGIONS OF THE HCV GENOM
US20130245102A1 (en) * 2010-10-12 2013-09-19 Research Foundation Of The City University Of New York Novel dna templates for small rna production in mammalian cells
CN102352360B (zh) * 2011-11-11 2012-10-10 南开大学 抑制hiv-1的双长发卡结构rna表达原件的构建及功能检测
WO2016100333A1 (en) * 2014-12-15 2016-06-23 Syngenta Participations Ag Pesticidal microrna carriers and use thereof

Also Published As

Publication number Publication date
CN112888785A (zh) 2021-06-01
PH12021500009A1 (en) 2021-09-13
UA129911C2 (uk) 2025-09-10
CA3108536A1 (en) 2020-02-06
KR20260036292A (ko) 2026-03-16
JP2024133533A (ja) 2024-10-02
AU2019313162A1 (en) 2021-03-18
KR20210054518A (ko) 2021-05-13
EP3830268A1 (en) 2021-06-09
MX2021001360A (es) 2021-07-16
US20210269800A1 (en) 2021-09-02
SG11202101059WA (en) 2021-02-25
AU2024203407A1 (en) 2024-08-01
IL280562A (en) 2021-03-25
BR112021001978A2 (pt) 2021-05-04
AU2019313162B2 (en) 2024-02-22
EP3830268A4 (en) 2023-12-13
WO2020024019A1 (en) 2020-02-06
MY210286A (en) 2025-09-08
JP2021533812A (ja) 2021-12-09

Similar Documents

Publication Publication Date Title
ZA202101256B (en) Rna molecules comprising non-canonical base pairs
IL288956A (en) RN" A building
ZA202101368B (en) Rna particles comprising polysarcosine
ZA201905396B (en) Trans-replicating rna
SG11201911430PA (en) Novel nucleic acid molecules
IL268894A (en) Medical RNA
SG11202003247RA (en) Rna sequence adaptation
SG10202108307YA (en) Rna cancer vaccines
HUE057821T2 (hu) Sejtmentes DNS fragmentációs mintázatának elemzése
IL277921A (en) RNA new small operators
SG11202006396SA (en) Immunomodulatory small hairpin rna molecules
EP3242510A4 (en) Base station
EP3361566A4 (en) BASE STATION
ZA202001649B (en) Rna molecules
IL289266A (en) new molecules
GB201907242D0 (en) Dna methods etc ii
EP3300422A4 (en) Base station
GB201818117D0 (en) Enantioselective process
HK40055918A (en) Rna molecules comprising non-canonical base pairs
HK40033373A (en) Rna molecules
AU2018902896A0 (en) RNA molecules
AU2018902840A0 (en) RNA molecules
GB201919193D0 (en) Novel molecules
AU2017903773A0 (en) RNA molecules
GB201912688D0 (en) Novel Molecules