BR112019015682A8 - Novas luciferases e métodos de uso destas - Google Patents
Novas luciferases e métodos de uso destasInfo
- Publication number
- BR112019015682A8 BR112019015682A8 BR112019015682A BR112019015682A BR112019015682A8 BR 112019015682 A8 BR112019015682 A8 BR 112019015682A8 BR 112019015682 A BR112019015682 A BR 112019015682A BR 112019015682 A BR112019015682 A BR 112019015682A BR 112019015682 A8 BR112019015682 A8 BR 112019015682A8
- Authority
- BR
- Brazil
- Prior art keywords
- methods
- proteins
- acid molecules
- nucleic acid
- nucleic acids
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 108060001084 Luciferase Proteins 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 102000004169 proteins and genes Human genes 0.000 abstract 6
- 210000004027 cell Anatomy 0.000 abstract 3
- 241000233866 Fungi Species 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 238000010353 genetic engineering Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 210000003463 organelle Anatomy 0.000 abstract 1
- 230000004850 protein–protein interaction Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
Abstract
novas luciferases e métodos de uso destas. a presente invenção refere-se a moléculas de ácido nucleico que codificam novas luciferases, fragmentos funcionais destas, homólogos e mutantes, assim como a proteínas codificadas pelos ditos ácidos nucleicos. as moléculas de ácido nucleico de interesse são isoladas de fungos ou obtidas por métodos de engenharia genética. da mesma forma, células hospedeiras, linhagens celulares estáveis e organismos transgênicos compreendendo as ditas moléculas de ácido nucleico são providos. além disso, anticorpos específicos das proteínas da presente invenção são providos. as ditas proteínas e ácidos nucleicos são usados em muitas aplicações e métodos, em particular, na marcação de organismos, células, organelas celulares ou proteínas. da mesma forma, as ditas composições de proteína e nucleotídeo são usadas em métodos para a detecção de interações proteína-proteína, para testar a atividade do promotor sob várias condições. finalmente, são providos kits para o uso de proteínas e ácidos nucleicos da presente invenção na diversidade de métodos e aplicações.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
RU2017102986A RU2674894C2 (ru) | 2017-01-30 | 2017-01-30 | Новые люциферазы и способы их использования |
PCT/RU2017/050125 WO2018139956A1 (ru) | 2017-01-30 | 2017-12-06 | Новые люциферазы и способы их использования |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112019015682A2 BR112019015682A2 (pt) | 2020-04-14 |
BR112019015682A8 true BR112019015682A8 (pt) | 2021-11-09 |
Family
ID=62979645
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112019015682A BR112019015682A8 (pt) | 2017-01-30 | 2017-12-06 | Novas luciferases e métodos de uso destas |
Country Status (11)
Country | Link |
---|---|
US (1) | US11913033B2 (pt) |
EP (1) | EP3575395A4 (pt) |
JP (1) | JP7298907B2 (pt) |
KR (1) | KR102594240B1 (pt) |
CN (1) | CN110234758A (pt) |
AU (1) | AU2017395644A1 (pt) |
BR (1) | BR112019015682A8 (pt) |
CA (1) | CA3051667A1 (pt) |
MX (1) | MX2019009045A (pt) |
RU (1) | RU2674894C2 (pt) |
WO (1) | WO2018139956A1 (pt) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2730038C2 (ru) | 2018-06-28 | 2020-08-14 | Общество с ограниченной ответственностью "ПЛАНТА" | Ферменты биосинтеза люциферина и их применение |
RU2744869C2 (ru) * | 2019-08-08 | 2021-03-16 | Общество с ограниченной ответственностью "ПЛАНТА" | Метод и реактивы для детекции активности люциферазы |
CN111560454B (zh) * | 2020-04-10 | 2022-08-16 | 上海交通大学 | 植物细胞高尔基体特异标记及应用 |
WO2024034619A1 (ja) * | 2022-08-10 | 2024-02-15 | 国立大学法人大阪大学 | 発光タンパク質 |
CN117567640A (zh) * | 2023-11-09 | 2024-02-20 | 苏州泓迅生物科技股份有限公司 | 一种在大肠杆菌宿主细胞中高效表达与纯化重组蛋白的融合标签肽及其应用 |
Family Cites Families (25)
Publication number | Priority date | Publication date | Assignee | Title |
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US5385938B1 (en) | 1986-12-23 | 1997-07-15 | Tristrata Inc | Method of using glycolic acid for treating wrinkles |
US5739409A (en) | 1987-06-19 | 1998-04-14 | The Regents Of The University Of California | Endogenously sweetened transgenic plant products |
US5256558A (en) | 1989-05-03 | 1993-10-26 | The Trustees Of Rockefeller University | Gene encoding plant asparagine synthetase |
US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
US5767367A (en) | 1990-06-23 | 1998-06-16 | Hoechst Aktiengesellschaft | Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation |
DE4207358A1 (de) | 1992-03-04 | 1993-09-09 | Inst Genbiologische Forschung | Expressionskassette und plasmide zur schliesszellenspezifischen expression und ihre verwendung zur herstellung transgener pflanzenzellen und pflanzen |
US5633155A (en) | 1992-08-19 | 1997-05-27 | Jinro Limited | Expression vector for phytolacca antiviral protein and process for preparing transgenic plant transformed therewith |
US5576198A (en) | 1993-12-14 | 1996-11-19 | Calgene, Inc. | Controlled expression of transgenic constructs in plant plastids |
US5750870A (en) | 1994-06-17 | 1998-05-12 | Agritope, Inc. | Plant genetic transformation methods and transgenic plants |
US5750868A (en) | 1994-12-08 | 1998-05-12 | Pioneer Hi-Bred International, Inc. | Reversible nuclear genetic system for male sterility in transgenic plants |
US5629470A (en) | 1995-01-20 | 1997-05-13 | Rutgers, The State University Of New Jersey | Transgenic plants and plant cells with enhanced pathogen resistance and related methods |
US5674731A (en) | 1995-04-27 | 1997-10-07 | Life Technologies, Inc. | Regeneration of both plant tissues and transgenic plant tissues using a new plant hormone, 5-bromoindole-3-acetic acid |
US5690045A (en) | 1996-10-10 | 1997-11-25 | Marine Automation, Inc. | Personal watercraft lift at transom |
AU772485C (en) * | 1999-10-26 | 2004-11-18 | Promega Corporation | Novel enzyme |
CA2328684A1 (en) * | 2000-12-15 | 2002-06-15 | Yahia Gawad | Photon-triggered luminescent assay |
EP1373481A4 (en) * | 2001-03-07 | 2005-04-13 | Xenogen Corp | PROCESS FOR SCREENING ON THE INTRODUCTION OF DNA TO A TARGET CELL |
AU2002307203A1 (en) * | 2001-04-06 | 2002-10-21 | The Scripps Research Institute | Bioluminescent plants and methods of making same |
US20050014687A1 (en) * | 2002-03-19 | 2005-01-20 | Anderson David W. | Therapeutic polypeptides, nucleic acids encoding same, and methods of use |
WO2004076685A2 (en) * | 2003-02-28 | 2004-09-10 | Lux Biotechnology Limited | Fungal biosensor and assay |
EP3000886A1 (en) * | 2003-03-19 | 2016-03-30 | Amgen Fremont Inc. | Antibodies against t cell immunoglobulin domain and mucin domain 1 (tim-1) antigen and uses thereof |
EP2512227B1 (en) * | 2009-12-17 | 2016-11-02 | Sanofi | Animal model expressing luciferase under control of the myelin basic protein promoter (mbp-luci) and use of the model for bioluminescence in vivo imaging |
CN106220739A (zh) * | 2010-12-09 | 2016-12-14 | 宾夕法尼亚大学董事会 | 嵌合抗原受体‑修饰的t细胞治疗癌症的用途 |
US9587266B2 (en) * | 2011-02-09 | 2017-03-07 | University Of Massachusetts | Mutant luciferases |
KR101346620B1 (ko) * | 2011-11-30 | 2014-01-06 | 전남대학교산학협력단 | 신규 박테리아 용해 단백질 및 그의 용도 |
IL293944A (en) * | 2012-08-20 | 2022-08-01 | Hutchinson Fred Cancer Res | Method and preparations for cellular immunotherapy |
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2017
- 2017-01-30 RU RU2017102986A patent/RU2674894C2/ru active
- 2017-12-06 US US16/479,693 patent/US11913033B2/en active Active
- 2017-12-06 AU AU2017395644A patent/AU2017395644A1/en active Pending
- 2017-12-06 MX MX2019009045A patent/MX2019009045A/es unknown
- 2017-12-06 CA CA3051667A patent/CA3051667A1/en active Pending
- 2017-12-06 BR BR112019015682A patent/BR112019015682A8/pt unknown
- 2017-12-06 EP EP17893668.8A patent/EP3575395A4/en active Pending
- 2017-12-06 JP JP2019538322A patent/JP7298907B2/ja active Active
- 2017-12-06 WO PCT/RU2017/050125 patent/WO2018139956A1/ru unknown
- 2017-12-06 KR KR1020197025169A patent/KR102594240B1/ko active IP Right Grant
- 2017-12-06 CN CN201780085183.3A patent/CN110234758A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
EP3575395A4 (en) | 2020-10-14 |
JP2020505028A (ja) | 2020-02-20 |
CN110234758A (zh) | 2019-09-13 |
MX2019009045A (es) | 2019-12-16 |
RU2674894C2 (ru) | 2018-12-13 |
US20210079363A1 (en) | 2021-03-18 |
US11913033B2 (en) | 2024-02-27 |
WO2018139956A1 (ru) | 2018-08-02 |
KR20190113860A (ko) | 2019-10-08 |
RU2017102986A (ru) | 2018-07-30 |
JP7298907B2 (ja) | 2023-06-27 |
BR112019015682A2 (pt) | 2020-04-14 |
EP3575395A1 (en) | 2019-12-04 |
KR102594240B1 (ko) | 2023-10-26 |
CA3051667A1 (en) | 2018-08-02 |
AU2017395644A1 (en) | 2019-09-12 |
RU2017102986A3 (pt) | 2018-07-30 |
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