BR112018013906A2 - modulação de acessibilidade de ácidos nucleicos hospedeiros a enzimas que digerem ácido nucleico em fluidos biológicos acelulares - Google Patents

modulação de acessibilidade de ácidos nucleicos hospedeiros a enzimas que digerem ácido nucleico em fluidos biológicos acelulares

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Publication number
BR112018013906A2
BR112018013906A2 BR112018013906A BR112018013906A BR112018013906A2 BR 112018013906 A2 BR112018013906 A2 BR 112018013906A2 BR 112018013906 A BR112018013906 A BR 112018013906A BR 112018013906 A BR112018013906 A BR 112018013906A BR 112018013906 A2 BR112018013906 A2 BR 112018013906A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
enzymes
modulation
biological fluids
accessibility
Prior art date
Application number
BR112018013906A
Other languages
English (en)
Inventor
Cabannes Éric
Original Assignee
Pathoquest
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pathoquest filed Critical Pathoquest
Publication of BR112018013906A2 publication Critical patent/BR112018013906A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)

Abstract

a presente invenção se refere um método para isolar, amplificar e sequenciar ácidos nucleicos dos agentes infecciosos de uma fração acelular de um fluido biológico com o uso de detergentes e enzimas de digestão de ácidos nucleicos. a presente invenção se refere adicionalmente a um kit de partes que compreende detergentes e enzimas de digestão de ácidos nucleicos para a implantação dos métodos de acordo com a presente invenção.
BR112018013906A 2016-01-08 2017-01-06 modulação de acessibilidade de ácidos nucleicos hospedeiros a enzimas que digerem ácido nucleico em fluidos biológicos acelulares BR112018013906A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662276650P 2016-01-08 2016-01-08
EP16150594 2016-01-08
PCT/EP2017/050251 WO2017118719A1 (en) 2016-01-08 2017-01-06 Modulation of accessibility of host nucleic acids to nucleic acid digesting enzymes in a cellular biological fluids

Publications (1)

Publication Number Publication Date
BR112018013906A2 true BR112018013906A2 (pt) 2018-12-18

Family

ID=55349630

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018013906A BR112018013906A2 (pt) 2016-01-08 2017-01-06 modulação de acessibilidade de ácidos nucleicos hospedeiros a enzimas que digerem ácido nucleico em fluidos biológicos acelulares

Country Status (7)

Country Link
EP (1) EP3400297B1 (pt)
KR (1) KR20180095082A (pt)
CN (1) CN109072231A (pt)
AU (1) AU2017205283A1 (pt)
BR (1) BR112018013906A2 (pt)
CA (1) CA3010751A1 (pt)
WO (2) WO2017118719A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110404113A (zh) * 2019-08-29 2019-11-05 上海白衣缘生物工程有限公司 一种制备无细胞医用植入材料的方法
GB201917101D0 (en) 2019-11-25 2020-01-08 Uea Enterprises Ltd Method for digesting nucleic acid in a sample
CN111662959A (zh) * 2020-07-22 2020-09-15 中国医学科学院病原生物学研究所 一种真菌高通量快速检测方法
CN114369640B (zh) * 2021-03-08 2024-09-06 南京诺唯赞生物科技股份有限公司 一种快速高效去除动物源性宿主核酸的方法
WO2024138484A1 (zh) * 2022-12-29 2024-07-04 深圳华大生命科学研究院 测序方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2354800A (en) * 1998-12-10 2000-06-26 Genetic Vectors, Inc. Method of protein removal
WO2005068662A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
KR100746372B1 (ko) * 2005-02-28 2007-08-03 바이오퀘스트(주) 핵산 분자가 포함되는 직접 효소 반응 방법
DE102005057334A1 (de) * 2005-11-28 2007-06-06 Aj Innuscreen Gmbh Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien
CN101548005A (zh) * 2006-10-06 2009-09-30 普罗梅加公司 分离细胞的方法和试剂盒
WO2009015484A1 (en) * 2007-08-02 2009-02-05 Universite Laval Concentration and enrichment of microbial cells and microbial nucleic acids from bodily fluids
WO2010062354A1 (en) * 2008-10-31 2010-06-03 Biomerieux, Inc. Methods for separation, characterization, and/or identification of microorganisms using mass spectrometry
EP2556143B1 (en) * 2010-04-08 2016-12-07 Qiagen GmbH Method for isolating and purifying nucleic acids
FR3001464B1 (fr) * 2013-01-25 2016-02-26 Biomerieux Sa Procede d'isolement specifique d'acides nucleiques d'interet
WO2015062699A1 (en) * 2013-10-30 2015-05-07 Merck Patent Gmbh Method for isolating microorganisms from a complex sample
US10655122B2 (en) * 2015-04-20 2020-05-19 Qiagen Gmbh Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample

Also Published As

Publication number Publication date
WO2017118719A1 (en) 2017-07-13
EP3400297B1 (en) 2022-09-14
KR20180095082A (ko) 2018-08-24
WO2017118719A8 (en) 2018-08-02
EP3400297A1 (en) 2018-11-14
AU2017205283A1 (en) 2018-07-19
CA3010751A1 (en) 2017-07-13
CN109072231A (zh) 2018-12-21
WO2017118718A1 (en) 2017-07-13

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]