BR112018008113A2 - veículo de cromatografia de afinidade e método para purificar a substância biológica - Google Patents

veículo de cromatografia de afinidade e método para purificar a substância biológica

Info

Publication number
BR112018008113A2
BR112018008113A2 BR112018008113-2A BR112018008113A BR112018008113A2 BR 112018008113 A2 BR112018008113 A2 BR 112018008113A2 BR 112018008113 A BR112018008113 A BR 112018008113A BR 112018008113 A2 BR112018008113 A2 BR 112018008113A2
Authority
BR
Brazil
Prior art keywords
affinity chromatography
group
affinity
biological material
refining method
Prior art date
Application number
BR112018008113-2A
Other languages
English (en)
Japanese (ja)
Inventor
Kihara Shiori
Original Assignee
Fujifilm Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujifilm Corporation filed Critical Fujifilm Corporation
Publication of BR112018008113A2 publication Critical patent/BR112018008113A2/pt

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
    • B01J20/28011—Other properties, e.g. density, crush strength
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/291—Gel sorbents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3202—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the carrier, support or substrate used for impregnation or coating
    • B01J20/3206—Organic carriers, supports or substrates
    • B01J20/3208—Polymeric carriers, supports or substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3291—Characterised by the shape of the carrier, the coating or the obtained coated product
    • B01J20/3293—Coatings on a core, the core being particle or fiber shaped, e.g. encapsulated particles, coated fibers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

trata-se de um veículo de cromatografia de afinidade que tem uma excelente pureza purificação, que inclui um substrato, um polímero hidrofí-lico e um ligante de afinidade, em que o substrato é constituído de pelo menos um selecionado a partir do grupo que consiste em um polissacarídeo, um polímero à base de acrilato, um polímero à base de metacrilato e um polímero à base de estire-no, o polímero hidrofílico é pelo menos um selecionado a partir do grupo que con-siste em polissacarídeos hidrofílicos, o ligante de afinidade é pelo menos um seleci-onado a partir do grupo que consiste em uma proteína de ligação ao anticorpo e um polipeptídeo de ligação ao anticorpo, um grupo carboxila é introduzido no veículo de cromatografia de afinidade e a quantidade do grupo carbóxi introduzida é de 15 mmol/l de gel para 60 mmol/l de gel em termos de capacidade de troca de íon.
BR112018008113-2A 2015-10-23 2016-10-21 veículo de cromatografia de afinidade e método para purificar a substância biológica BR112018008113A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2015-208982 2015-10-23
JP2015208982 2015-10-23
PCT/JP2016/081312 WO2017069254A1 (ja) 2015-10-23 2016-10-21 アフィニティクロマトグラフィ担体および生体物質の精製方法

Publications (1)

Publication Number Publication Date
BR112018008113A2 true BR112018008113A2 (pt) 2018-11-06

Family

ID=58557258

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018008113-2A BR112018008113A2 (pt) 2015-10-23 2016-10-21 veículo de cromatografia de afinidade e método para purificar a substância biológica

Country Status (11)

Country Link
US (1) US10888841B2 (pt)
EP (1) EP3367093B1 (pt)
JP (1) JP6600002B2 (pt)
KR (1) KR20180049026A (pt)
CN (1) CN108139371A (pt)
AU (1) AU2016340518B2 (pt)
BR (1) BR112018008113A2 (pt)
CA (1) CA3000965A1 (pt)
RU (1) RU2694637C1 (pt)
SG (1) SG11201802997WA (pt)
WO (1) WO2017069254A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201616758D0 (en) * 2016-10-03 2016-11-16 Ge Healthcare Bio Sciences Ab Novel chromatography media
WO2018105198A1 (ja) * 2016-12-09 2018-06-14 富士フイルム株式会社 ミックスモードアフィニティクロマトグラフィー用担体
EP3894428A4 (en) 2018-12-12 2022-09-07 Purilogics, LLC AFFINITY MEMBRANE AND PROCESS OF PRODUCTION
CN113926434A (zh) * 2021-11-03 2022-01-14 上海江夏血液技术有限公司 一种蛋白吸附膜材料及其制备方法和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4663163A (en) * 1983-02-14 1987-05-05 Hou Kenneth C Modified polysaccharide supports
JPH075688B2 (ja) * 1983-02-14 1995-01-25 キュノ、インコーポレーテッド 変性多糖支持体
JPS6348222A (ja) * 1986-08-19 1988-02-29 Showa Denko Kk インタ−ロイキン−２活性阻害因子吸着剤
GB2195344B (en) * 1986-08-19 1991-05-08 Showa Denko Kk Adsorbent composed of porous beads of chitosan and adsorption method using same
SU1700006A1 (ru) * 1989-02-06 1991-12-23 Институт биоорганической химии АН БССР Способ получени аффинных сорбентов дл иммуноферментного анализа
BRPI0515586B1 (pt) * 2004-09-22 2015-11-03 Ge Healthcare Bio Sciences Ab método de preparar uma matriz cromatográfica de polissacarídeo reticulado, matriz cromatográfica porosa de polissacarídeo reticulado, e, uso de uma matriz cromatográfica
EP2167229A4 (en) * 2007-07-25 2012-02-01 Ge Healthcare Bio Sciences Ab SEPARATION MATRIX
JP2010133734A (ja) * 2008-12-02 2010-06-17 Tosoh Corp アフィニティークロマトグラフィー用カルボキシル化担体、及びそれを用いたアフィニティークロマトグラフィー用分離剤
US8912117B2 (en) 2009-02-20 2014-12-16 Jnc Corporation Cellulose gel for purification of immunoglobulin
US9890191B2 (en) 2012-09-03 2018-02-13 Kaneka Corporation Mixed-mode antibody affinity separation matrix and purification method using the same, and the target molecules
WO2014171437A1 (ja) * 2013-04-16 2014-10-23 旭化成メディカル株式会社 抗体タンパク質の精製方法
CN105555795A (zh) * 2013-09-17 2016-05-04 株式会社钟化 新抗体纯化方法和由该方法得到的抗体、以及使用了阳离子交换基团的新抗体纯化法和由该方法得到的抗体

Also Published As

Publication number Publication date
AU2016340518B2 (en) 2019-02-28
KR20180049026A (ko) 2018-05-10
SG11201802997WA (en) 2018-05-30
EP3367093B1 (en) 2021-11-03
CN108139371A (zh) 2018-06-08
US20180229215A1 (en) 2018-08-16
EP3367093A1 (en) 2018-08-29
EP3367093A4 (en) 2018-10-17
US10888841B2 (en) 2021-01-12
RU2694637C1 (ru) 2019-07-16
WO2017069254A1 (ja) 2017-04-27
AU2016340518A1 (en) 2018-04-26
JPWO2017069254A1 (ja) 2018-07-19
JP6600002B2 (ja) 2019-10-30
CA3000965A1 (en) 2017-04-27

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]