BR112018003746A2 - métodos para inativação e extracção de bactéria álcool-ácido resistente a partir de meios líquidos para caracterização e/ou identificação utilizando espectrometria de massa - Google Patents

métodos para inativação e extracção de bactéria álcool-ácido resistente a partir de meios líquidos para caracterização e/ou identificação utilizando espectrometria de massa

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Publication number
BR112018003746A2
BR112018003746A2 BR112018003746A BR112018003746A BR112018003746A2 BR 112018003746 A2 BR112018003746 A2 BR 112018003746A2 BR 112018003746 A BR112018003746 A BR 112018003746A BR 112018003746 A BR112018003746 A BR 112018003746A BR 112018003746 A2 BR112018003746 A2 BR 112018003746A2
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BR
Brazil
Prior art keywords
acid
resistant bacteria
tube
inactivation
mass spectrometry
Prior art date
Application number
BR112018003746A
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English (en)
Other versions
BR112018003746B1 (pt
Inventor
Miller Eric
Moreno Erik
Totty Heather
Deol Parampal
Original Assignee
Bio Merieux Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Merieux Inc filed Critical Bio Merieux Inc
Publication of BR112018003746A2 publication Critical patent/BR112018003746A2/pt
Publication of BR112018003746B1 publication Critical patent/BR112018003746B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/35—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Mycobacteriaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/355—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Nocardia (G)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Sustainable Development (AREA)
  • Optics & Photonics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção é direcionada a um método e um kit para a inativação de bactérias álcool-ácido resistentes. em algumas modalidades, o método pode in-cluir: transferir uma amostra de uma cultura líquida contendo bactérias álcool-ácido resistentes para um primeiro tubo, em que o primeiro tubo compreende um corpo, uma primeira extremidade ao corpo tendo uma abertura, e uma segunda extremida-de ao corpo tendo uma porção frustocônica terminando em uma ponta côncava; centrifugar o tubo para concentrar a bactéria álcool-ácido resistente na ponta côn-cava e subsequentemente decantar um sobrenadante; ressuspensão do concen-trado bacteriano álcool-ácido resistente em álcool; transferir a suspensão para um segundo tubo contendo esferas; agitar o segundo tubo para romper as células de bactérias álcool-ácido resistentes; e incubação da suspensão para inativar a bacté-ria álcool-ácido resistente na amostra de teste. o método também pode incluir identi-ficar as bactérias álcool-ácido resistentes com espectrometria de massa.
BR112018003746-0A 2015-08-24 2016-07-15 Métodos para inativação e extração de bactéria álcool- ácido resistente em uma amostra de teste para caracterização e/ou identificação utilizando espectrometria de massa BR112018003746B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201562209116P 2015-08-24 2015-08-24
US62/209,116 2015-08-24
US201662306390P 2016-03-10 2016-03-10
US62/306,390 2016-03-10
PCT/US2016/042537 WO2017034699A1 (en) 2015-08-24 2016-07-15 Methods for inactivation and extraction of acid-fast bacteria from liquid media for characterization and/or identification using mass spectrometry

Publications (2)

Publication Number Publication Date
BR112018003746A2 true BR112018003746A2 (pt) 2018-09-25
BR112018003746B1 BR112018003746B1 (pt) 2024-03-12

Family

ID=56550414

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018003746-0A BR112018003746B1 (pt) 2015-08-24 2016-07-15 Métodos para inativação e extração de bactéria álcool- ácido resistente em uma amostra de teste para caracterização e/ou identificação utilizando espectrometria de massa

Country Status (8)

Country Link
US (1) US10626437B2 (pt)
EP (1) EP3341491A1 (pt)
JP (2) JP2018527925A (pt)
CN (1) CN108291247A (pt)
AU (1) AU2016312197B2 (pt)
BR (1) BR112018003746B1 (pt)
CA (1) CA2996498A1 (pt)
WO (1) WO2017034699A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3647763B1 (en) * 2018-10-29 2021-07-14 FEI Company A method of preparing a biological sample for study in an analysis device
CN117092199B (zh) * 2023-10-16 2024-01-26 成都泰莱医学检验实验室有限公司 利用碳化钨纳米材料基质进行飞行时间质谱检测的方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5253551A (en) * 1990-07-12 1993-10-19 Bio-Pias, Inc. Centrifuge tube and centrifuge tube cap removing and installing tool and method
US6177266B1 (en) 1996-04-29 2001-01-23 The United States Of America As Represented By The Secretary Of The Army Rapid identification of bacteria by mass spectrometry
US6723564B2 (en) 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US5948610A (en) 1998-06-03 1999-09-07 University Of Maryland At Baltimore County Use of matrices comprising liquids and light absorbing particles for analysis of microorganisms by laser desorption mass spectrometry
US7020559B1 (en) 1998-11-17 2006-03-28 University Of Maryland Methods for identifying and classifying organisms by mass spectrometry and database searching
US20020192676A1 (en) 2001-06-18 2002-12-19 Madonna Angelo J. Method for determining if a type of bacteria is present in a mixture
US6833249B2 (en) 2002-03-22 2004-12-21 Council Of Scientific And Industrial Research Quick and sensitive method of quantifying mycolic acid to develop anti-microbial agents and a diagnostic kit thereof
KR20050052467A (ko) 2002-08-12 2005-06-02 다이나박스 테크놀로지 코퍼레이션 면역조절 조성물, 이의 제조방법 및 이의 이용방법
MXPA05005031A (es) 2002-11-12 2005-11-17 Becton Dickinson Co Diagnostico de la sepsis o sirs usando perfiles de biomarcadores.
DE102006021493B4 (de) 2006-05-09 2010-04-01 Bruker Daltonik Gmbh Massenspektrometrische Messung mikrobieller Resistenzen
DE102007058516B4 (de) 2007-11-23 2017-08-10 Bruker Daltonik Gmbh Identifizierung von Erregern in Körperflüssigkeiten
US20110268744A1 (en) 2008-05-26 2011-11-03 Ian Garthwaite Method of Diagnosis of Infection by Mycobacteria and Reagents Therefor
MX2011004108A (es) * 2008-10-31 2011-08-15 Bio Merieux Inc Metodos para la separacion, caracterizacion y/o identificacion de microorganismos utilizando espectrometria de masas.
US8647835B2 (en) * 2008-10-31 2014-02-11 BIO MéRIEUX, INC. Methods for separation, characterization and/or identification of microorganisms using spectroscopy
CN102414326A (zh) 2009-02-26 2012-04-11 泰里安诊断有限公司 诊断分枝杆菌感染的方法及用于该方法的试剂
WO2011058131A1 (en) * 2009-11-12 2011-05-19 Centre Hospitalier Universitaire Vaudois (Chuv) Methods of producing a bacterial preparation
US20130309716A1 (en) 2012-05-17 2013-11-21 Biomerieux Inc. Methods For Inactiviation And/or Extraction of A Fungus Test Sample For Characterization And/or Identification Using Mass Spectrometry
US8735091B2 (en) * 2012-05-17 2014-05-27 Biomerieux, Inc. Methods for inactivation and extraction of acid-fast bacteria for characterization and/or identification using mass spectrometry
US9932620B2 (en) * 2012-05-21 2018-04-03 Celsis Ltd. Methods, devices, and systems of detecting microorganisms
CN103308696B (zh) * 2013-05-30 2015-06-24 中国疾病预防控制中心传染病预防控制所 基于质谱技术的布鲁氏菌快速检测试剂盒

Also Published As

Publication number Publication date
EP3341491A1 (en) 2018-07-04
WO2017034699A1 (en) 2017-03-02
US20170058316A1 (en) 2017-03-02
CA2996498A1 (en) 2017-03-02
BR112018003746B1 (pt) 2024-03-12
AU2016312197B2 (en) 2022-08-18
US10626437B2 (en) 2020-04-21
AU2016312197A1 (en) 2018-03-15
CN108291247A (zh) 2018-07-17
JP2021097707A (ja) 2021-07-01
JP2018527925A (ja) 2018-09-27
JP7055913B2 (ja) 2022-04-18

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Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 15/07/2016, OBSERVADAS AS CONDICOES LEGAIS