BR112017008251A2 - usos de um tecido de um porco transgênico que compreende um gene a(1,3) galactosiltransferase, cmah e ß4galnt2 interrompido e de um produto relacionado com a pele, métodos para preparação de um material de transplante para xenoenxerto, para produção de um composto de interesse e para produzir um reagente de cultura de células, e, reagente de cultura de células - Google Patents

usos de um tecido de um porco transgênico que compreende um gene a(1,3) galactosiltransferase, cmah e ß4galnt2 interrompido e de um produto relacionado com a pele, métodos para preparação de um material de transplante para xenoenxerto, para produção de um composto de interesse e para produzir um reagente de cultura de células, e, reagente de cultura de células

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Publication number
BR112017008251A2
BR112017008251A2 BR112017008251A BR112017008251A BR112017008251A2 BR 112017008251 A2 BR112017008251 A2 BR 112017008251A2 BR 112017008251 A BR112017008251 A BR 112017008251A BR 112017008251 A BR112017008251 A BR 112017008251A BR 112017008251 A2 BR112017008251 A2 BR 112017008251A2
Authority
BR
Brazil
Prior art keywords
cell culture
culture reagent
methods
interest
disrupted
Prior art date
Application number
BR112017008251A
Other languages
English (en)
Other versions
BR112017008251B1 (pt
Inventor
A Tector Joseph
Original Assignee
Univ Indiana Res & Tech Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Indiana Res & Tech Corp filed Critical Univ Indiana Res & Tech Corp
Publication of BR112017008251A2 publication Critical patent/BR112017008251A2/pt
Publication of BR112017008251B1 publication Critical patent/BR112017008251B1/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/22—Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biomedical Technology (AREA)
  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

o pedido provê métodos para melhorar um sintoma relacionado com a rejeição, para reduzir a separação prematura e métodos para produção de um composto de interesse com um perfil de epítopo alterado. são fornecidos porcos knockout com um gene ou genes interrompidos, e órgãos, tecidos e células porcinas a partir dos mesmos.
BR112017008251-9A 2014-10-22 2015-10-21 Usos de um tecido de um porco transgênico que compreende um gene a(1,3) galactosiltransferase, cmah e ß4galnt2 interrompido e de um produto relacionado com a pele, e, métodos para preparação de um material de transplante para xenoenxerto, para produção de um composto de interesse e para produzir um reagente de cultura de células BR112017008251B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462067129P 2014-10-22 2014-10-22
US62/067129 2014-10-22
PCT/US2015/056730 WO2016065046A1 (en) 2014-10-22 2015-10-21 Triple transgenic pigs suitable for xenograft

Publications (2)

Publication Number Publication Date
BR112017008251A2 true BR112017008251A2 (pt) 2018-05-02
BR112017008251B1 BR112017008251B1 (pt) 2022-02-22

Family

ID=55761497

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017008251-9A BR112017008251B1 (pt) 2014-10-22 2015-10-21 Usos de um tecido de um porco transgênico que compreende um gene a(1,3) galactosiltransferase, cmah e ß4galnt2 interrompido e de um produto relacionado com a pele, e, métodos para preparação de um material de transplante para xenoenxerto, para produção de um composto de interesse e para produzir um reagente de cultura de células

Country Status (8)

Country Link
US (1) US20170311579A1 (pt)
EP (2) EP4129308A1 (pt)
JP (3) JP2017536814A (pt)
KR (2) KR102659529B1 (pt)
CN (2) CN116059250A (pt)
BR (1) BR112017008251B1 (pt)
CA (1) CA2965550A1 (pt)
WO (1) WO2016065046A1 (pt)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
JP6830437B2 (ja) * 2014-12-10 2021-02-17 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ 疾患を処置するための遺伝子改変された細胞、組織および臓器
EP3313176A4 (en) * 2015-06-26 2019-06-26 Indiana University Research&technology Corporation TRANSGENIC PORKS HAVING GENETIC MODIFICATIONS OF ALS
EP3347458A4 (en) * 2015-09-09 2019-08-07 Revivicor Inc. MULTI TRANSGENIC PIG FOR XENOTRANSPLANTATION
AU2017285224B2 (en) * 2016-06-14 2023-05-18 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease
WO2018157011A1 (en) * 2017-02-24 2018-08-30 Xenotherapeutics, Inc. Products and methods for treating burn wounds
EP3510861A3 (en) 2017-12-22 2019-09-25 Avantea SRL Hypoallergenic food and medical products from genome edited livestock
KR20200119826A (ko) * 2018-01-30 2020-10-20 인디애나 유니버시티 리서치 앤드 테크놀로지 코포레이션 돼지 이종항원의 식별
CN108220294A (zh) * 2018-02-11 2018-06-29 南京医科大学 CRISPR/Cas9载体组合及其在基因敲除中的应用
CN108588123A (zh) * 2018-05-07 2018-09-28 南京医科大学 CRISPR/Cas9载体组合在制备基因敲除猪的血液制品中的应用
WO2020072982A1 (en) 2018-10-05 2020-04-09 Xenotherapeutics, Inc. Xenotransplantation products and methods
US10883084B2 (en) 2018-10-05 2021-01-05 Xenotherapeutics, Inc. Personalized cells, tissues, and organs for transplantation from a humanized, bespoke, designated-pathogen free, (non-human) donor and methods and products relating to same
AU2019359400A1 (en) * 2018-10-10 2021-05-20 Revivicor, Inc. Compositions and methods for preventing allergies
EP3945799A1 (en) 2019-03-25 2022-02-09 Xenotherapeutics, Inc. Personalized cells, tissues, and organs for transplantation from a humanized, bespoke, designated-pathogen free, (non- human) donor and methods and products relating to same
KR102176161B1 (ko) * 2019-07-23 2020-11-09 주식회사 옵티팜 돼지 내인성 레트로바이러스 Envlope C 음성, GGTA1, CMAH, iGb3s, β4GalNT2 유전자가 넉아웃되고, 인간 CD46 및 TBM 유전자를 발현하는 이종장기이식을 위한 형질전환 복제돼지 및 이의 제조방법
WO2021024123A1 (en) * 2019-08-06 2021-02-11 Nzeno Limited Donor pigs for xenotransplantation
US20220409669A1 (en) * 2019-12-02 2022-12-29 The General Hospital Corporation Nerve Xenografts and Related Methods
CN113425906A (zh) * 2020-03-23 2021-09-24 成都中科奥格生物科技有限公司 一种软骨材料及其制备方法和用途
CN111778251A (zh) * 2020-07-14 2020-10-16 金佩奇生物科技(南京)有限公司 敲除猪异种抗原的基因的gRNA及其应用
CN113512534B (zh) * 2020-09-23 2024-04-23 杭州启函生物科技有限公司 用于遗传修饰和靶向的组合物和方法
US20230203176A1 (en) 2021-09-17 2023-06-29 Novartis Ag Methods For Prevention Of Graft Rejection In Xenotransplantation

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6166288A (en) 1995-09-27 2000-12-26 Nextran Inc. Method of producing transgenic animals for xenotransplantation expressing both an enzyme masking or reducing the level of the gal epitope and a complement inhibitor
AU761831B2 (en) 1998-04-15 2003-06-12 Bioscience 2002 Llc Inhibition of xenoreactive antibodies
US6650968B2 (en) 2000-12-27 2003-11-18 Plug Power Inc. Technique to regulate an efficiency of a fuel cell system
CA2445945A1 (en) 2001-04-30 2002-11-07 Rbc Biotechnology, Inc. Modified organs and cells for xenotransplantation
CA2471035C (en) 2001-12-21 2014-05-13 The Curators Of The University Of Missouri Knockout swine and methods for making the same
CA2496761C (en) 2002-08-21 2015-06-02 Revivicor, Inc. Porcine animals lacking any expression of functional alpha 1,3 galactosyltransferase
EP1685148A2 (en) 2003-11-05 2006-08-02 University Of Pittsburgh Of The Commonwealth System Of Higher Education PORCINE ISOGLOBOSIDE 3 SYNTHASE PROTEIN, cDNA, GENOMIC ORGANIZATION, AND REGULATORY REGION
US9642899B2 (en) * 2010-05-06 2017-05-09 Mayo Foundation For Medical Education And Research Implantation of a cardiac xenograft from a B4GALNT2KO and GTKO transgenic pig to reduce immunogenicity
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues

Also Published As

Publication number Publication date
EP3220925A4 (en) 2018-07-18
EP3220925A1 (en) 2017-09-27
KR20240055155A (ko) 2024-04-26
EP3220925B1 (en) 2022-03-16
JP2017536814A (ja) 2017-12-14
BR112017008251B1 (pt) 2022-02-22
EP4129308A1 (en) 2023-02-08
CN107106607A (zh) 2017-08-29
KR20170074941A (ko) 2017-06-30
WO2016065046A1 (en) 2016-04-28
US20170311579A1 (en) 2017-11-02
CN116059250A (zh) 2023-05-05
JP2023109993A (ja) 2023-08-08
KR102659529B1 (ko) 2024-04-23
JP2021045134A (ja) 2021-03-25
CA2965550A1 (en) 2016-04-28
WO2016065046A9 (en) 2017-06-29

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Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 21/10/2015, OBSERVADAS AS CONDICOES LEGAIS.