BR112017003608A2 - ?método para a análise comparativa do nível de expressão de mirna(s) alvo(s), dispositivo de análise da expressão de mirna, programa(s) para uma análise comparativa do(s) nível(eis) de expressão de mirna(s), meio de gravação legível por computador e chip para análise da expressão de mirna? - Google Patents

?método para a análise comparativa do nível de expressão de mirna(s) alvo(s), dispositivo de análise da expressão de mirna, programa(s) para uma análise comparativa do(s) nível(eis) de expressão de mirna(s), meio de gravação legível por computador e chip para análise da expressão de mirna?

Info

Publication number
BR112017003608A2
BR112017003608A2 BR112017003608A BR112017003608A BR112017003608A2 BR 112017003608 A2 BR112017003608 A2 BR 112017003608A2 BR 112017003608 A BR112017003608 A BR 112017003608A BR 112017003608 A BR112017003608 A BR 112017003608A BR 112017003608 A2 BR112017003608 A2 BR 112017003608A2
Authority
BR
Brazil
Prior art keywords
mirna
analysis
mirna expression
comparative analysis
expression level
Prior art date
Application number
BR112017003608A
Other languages
English (en)
Other versions
BR112017003608B1 (pt
Inventor
Kozono Satoko
Kondou Satoshi
Original Assignee
Toray Industries
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toray Industries filed Critical Toray Industries
Publication of BR112017003608A2 publication Critical patent/BR112017003608A2/pt
Publication of BR112017003608B1 publication Critical patent/BR112017003608B1/pt

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
    • G16B40/20—Supervised data analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Evolutionary Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Data Mining & Analysis (AREA)
  • Sustainable Development (AREA)
  • Bioethics (AREA)
  • Software Systems (AREA)
  • Databases & Information Systems (AREA)
  • Epidemiology (AREA)
  • Artificial Intelligence (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Public Health (AREA)

Abstract

é divulgado um método para a análise comparativa dos níveis de expressão de mirnas alvos entre uma pluralidade de amostras de fluido corporal. no método para a análise comparativa da presente invenção, os níveis de expressão de mirnas alvos em cada amostra de fluido corporal são corrigidos utilizando o(s) nível(eis) de expressão de mirna(s) endógeno(s) de correção que é(são) mensurado(s) simultaneamente com os níveis de expressão dos mirnas alvos na amostra. como mirna(s) endógeno(s) de correção, um ou mais mirnas selecionados a partir de 10 tipos específicos de mirnas endógenos de correção são utilizados. pela presente invenção, a análise comparativa dos mirnas alvos nas amostras de fluido corporal pode ser realizada com uma precisão maior do que por meio das técnicas convencionais.
BR112017003608-8A 2014-09-16 2015-09-15 MÉTODO PARA A ANÁLISE COMPARATIVA DO NÍVEL DE EXPRESSÃO DE miRNA(S) ALVO(S) E CHIP PARA ANÁLISE DA EXPRESSÃO DE miRNA BR112017003608B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2014187685 2014-09-16
JP2014-187685 2014-09-16
PCT/JP2015/076085 WO2016043170A1 (ja) 2014-09-16 2015-09-15 ｍｉＲＮＡ発現量の比較解析方法及び装置

Publications (2)

Publication Number Publication Date
BR112017003608A2 true BR112017003608A2 (pt) 2018-01-30
BR112017003608B1 BR112017003608B1 (pt) 2023-11-07

Family

ID=55533207

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017003608-8A BR112017003608B1 (pt) 2014-09-16 2015-09-15 MÉTODO PARA A ANÁLISE COMPARATIVA DO NÍVEL DE EXPRESSÃO DE miRNA(S) ALVO(S) E CHIP PARA ANÁLISE DA EXPRESSÃO DE miRNA

Country Status (8)

Country Link
US (1) US11348663B2 (pt)
EP (1) EP3196316B1 (pt)
JP (1) JP6819848B2 (pt)
KR (1) KR102350747B1 (pt)
CN (1) CN107075578B (pt)
BR (1) BR112017003608B1 (pt)
CA (1) CA2957267C (pt)
WO (1) WO2016043170A1 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019168933A1 (en) 2018-02-27 2019-09-06 Quadrant Biosciences Inc. A method for monitoring metabolism before and after exercise or injury using salivary micro-rnas
JP7363478B2 (ja) * 2018-07-31 2023-10-18 東レ株式会社 体液検体の品質評価方法
JP2022053549A (ja) * 2019-01-31 2022-04-06 国立大学法人 東京医科歯科大学 心房性不整脈検査方法及び心房性不整脈検査システム
CN113151413A (zh) * 2021-04-28 2021-07-23 杭州觅因生物科技有限公司 一种具有校准功能的microRNA检测试剂盒

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007075095A (ja) 2005-04-11 2007-03-29 Mitsubishi Rayon Co Ltd ｍｉＲＮＡ搭載マイクロアレイ
JP2007097429A (ja) 2005-09-30 2007-04-19 Mitsubishi Rayon Co Ltd ｎｏｎ−ｃｏｄｉｎｇＲＮＡの検出データ補正方法
US7993831B2 (en) 2007-09-14 2011-08-09 Asuragen, Inc. Methods of normalization in microRNA detection assays
JP5750710B2 (ja) 2008-04-30 2015-07-22 日本電気株式会社 癌マーカー、それを用いた癌の評価方法および評価試薬
CN102892898B (zh) 2009-12-24 2016-03-09 复旦大学 用于肝细胞癌诊断的含微rna生物标记的诊断试剂盒和方法
WO2011076141A1 (en) 2009-12-24 2011-06-30 Fudan University Diagnostic kits comprising microrna biomarkers and methods for diagnosis of hepatocellular cancer
CN102071253A (zh) 2010-12-08 2011-05-25 中国人民解放军第二军医大学 检测粪便中microRNA表达水平的方法和内参基因的选择方法
JP5998674B2 (ja) * 2012-06-29 2016-09-28 東レ株式会社 小型ｒｎａ発現量の比較解析方法
CN104540966A (zh) 2012-08-27 2015-04-22 国立大学法人京都大学 通过微小rna诊断类风湿性关节炎
WO2014048441A1 (en) * 2012-09-26 2014-04-03 Mirrx Therapeutics Oligomers with improved off-target profile

Also Published As

Publication number Publication date
US20170262581A1 (en) 2017-09-14
US11348663B2 (en) 2022-05-31
BR112017003608B1 (pt) 2023-11-07
CN107075578B (zh) 2021-06-01
EP3196316A1 (en) 2017-07-26
JP6819848B2 (ja) 2021-01-27
KR20170052530A (ko) 2017-05-12
EP3196316A9 (en) 2018-10-24
JPWO2016043170A1 (ja) 2017-06-22
EP3196316A4 (en) 2018-03-28
EP3196316B1 (en) 2023-02-22
KR102350747B1 (ko) 2022-01-13
WO2016043170A1 (ja) 2016-03-24
CA2957267A1 (en) 2016-03-24
CA2957267C (en) 2023-03-14
CN107075578A (zh) 2017-08-18

Similar Documents

Publication Publication Date Title
BR112018002848A2 (pt) método, aparelho, mídia de armazenamento não transitório, programa de computador, kit para medir níveis de expressão de seis ou mais genes-alvo da via de sinalização celular
BR112017007965A2 (pt) método implantado por computador para inferir a atividade de uma via de sinalização celular de tgf-b em um indivíduo; aparelho para inferir a atividade de uma via de sinalização celular de tgf-b em um indivíduo; meio de armazenamento não transitório; programa de computador; kits para medir níveis de expressão de três ou mais genes-alvo da via de sinalização celular de tgf-b em uma amostra de um indivíduo; inferir a atividade de uma via de sinalização celular de tgf-b em um indivíduo; inferir a atividade de uma via de sinalização celular de tgf-b em um indivíduo; e uso do kit
BR112018013550A2 (pt) identificação de entidades utilizando um modelo de aprendizado profundo
BR112016003868A2 (pt) método, aparelho, mídia de armazenamento não transitório, e, programa de computador
BR112017007962A2 (pt) método implementado por computador, aparelho, mídia de armazenamento, programa de computador, kit, e, uso do kit
BR112018069849A2 (pt) kit, dispositivo e método para a detecção de câncer pancreático precoce ou de lesão precursora do câncer pancreático
BR112018003631A2 (pt) sistemas e métodos para busca por variante de alta precisão
BR112017017025A2 (pt) métodos e composições para analizar componentes celulares
BR112014008597A2 (pt) sistema e método para determinação dos pontos q e j de um eletrocardiograma (ecg); um ou mais processadores; e mídia executável por computador não transitória
DK3129493T3 (da) Import af unaturlige eller modificerede nukleosidtriphosphater ind i celler via nukleinsyre-triphosphat-transportører
BR112015019570A8 (pt) método, sistema e meio de armazenamento por computador para manter compatibilidade de aplicativo com base em restrições de conjunto de atribuições
BR112017014116A2 (pt) pcr quantitativo multiplex
BR112016002435A2 (pt) proteínas biomarcadoras da síndrome urêmica hemolítica atípica
BR112016024494A2 (pt) análogos de peptídeo com sonda(s) de aminoácidos ramificados
BR112016024187A2 (pt) sistema sensor de fibra óptica, e, método para a determinação de uma localização de uma perturbação
BR112017003608A2 (pt) ?método para a análise comparativa do nível de expressão de mirna(s) alvo(s), dispositivo de análise da expressão de mirna, programa(s) para uma análise comparativa do(s) nível(eis) de expressão de mirna(s), meio de gravação legível por computador e chip para análise da expressão de mirna?
EA201790769A1 (ru) Определение уровней гликозаминогликанов методом масс-спектрометрии
BR112017016897A2 (pt) cepas fúngicas e métodos de uso
BR112015018213A2 (pt) método para determinar a síndrome da angústia respiratória aguda (ards) relacionada aos biomarcadores, um método para monitorar o desenvolvimento e o tratamento da ards em um paciente
BR112016028534A2 (pt) ceramidas e seu uso em diagnosticar cvd
BR112017025459A2 (pt) composição, método para tratamento de doença falciforme, kit, método para detecção de polissulfato pentosano de sódio (pps)
WO2016130572A3 (en) Methods of determining levels of exposure to radiation and uses thereof
BR112014003877A2 (pt) biomarcadores de micro-rna e métodos de uso dos mesmos
BR102012002812A8 (pt) método para determinar a influência de uma variável em um fenômeno
BR112016016249A2 (pt) Métodos de análise imunocromatográfica, dispositivo de análise imunocromatográfica e kit

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 15/09/2015, OBSERVADAS AS CONDICOES LEGAIS