BR112014031143B8 - Método para quantificar células imunes em tecidos tumorais e suas aplicações - Google Patents

Método para quantificar células imunes em tecidos tumorais e suas aplicações

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Publication number
BR112014031143B8
BR112014031143B8 BR112014031143A BR112014031143A BR112014031143B8 BR 112014031143 B8 BR112014031143 B8 BR 112014031143B8 BR 112014031143 A BR112014031143 A BR 112014031143A BR 112014031143 A BR112014031143 A BR 112014031143A BR 112014031143 B8 BR112014031143 B8 BR 112014031143B8
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BR
Brazil
Prior art keywords
immune cells
tumor
tumor tissues
applications
analyzed
Prior art date
Application number
BR112014031143A
Other languages
English (en)
Other versions
BR112014031143A2 (pt
BR112014031143B1 (pt
Inventor
Milecnik Bernhard
Galon Jérôme
Pagès Franck
Original Assignee
Univ Paris Descartes
Inst Nat Sante Rech Med
Hopitaux Paris Assist Publique
Univ Paris
Univ Paris Cite
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Publication date
Application filed by Univ Paris Descartes, Inst Nat Sante Rech Med, Hopitaux Paris Assist Publique, Univ Paris, Univ Paris Cite filed Critical Univ Paris Descartes
Publication of BR112014031143A2 publication Critical patent/BR112014031143A2/pt
Publication of BR112014031143B1 publication Critical patent/BR112014031143B1/pt
Publication of BR112014031143B8 publication Critical patent/BR112014031143B8/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODO PARA QUANTIFICAR CÉLULAS IMUNES EM TECIDOS TUMORAIS E SUAS APLICAÇÕES. Um método para avaliação de um número ou a densidade de células imunológicas em tecidos tumorais compreendendo as etapas que consistem em: a. fornecer uma ou mais lâminas imunocoradas de secção de tecido obtidas por um sistema de coloração de lâminas automatizado usando anticorpos que se ligam especificamente a antígenos (marcadores) expressos pelas células imunológicas. b. proceder à digitalização das lâminas da etapa a. pela captura de imagem alta resolução, pelo qual uma imagem digital de alta definição (4,6 mm / pixel ou melhor) da lâmina a ser analisada é obtida, c. detectar a porção de corte de tecido sobre a imagem digital d. analisar a porção da seção de tecido para a definição (i) do tumor (TC) e (ii) da margem invasiva do tumor (IM), e. fornecer uma grade de referência de tamanho com unidades uniformemente distribuídas tendo uma mesma superfície, a dita grade sendo adaptada ao tamanho do tumor a ser analisado, e1. verificar a qualidade da immunocoloração, f. detectar e quantificar as células coradas de cada unidade em que o número ou a densidade de células imunológicas coradas de cada unidade é avaliada.
BR112014031143A 2012-06-14 2013-06-14 Método para quantificar células imunes em tecidos tumorais e suas aplicações BR112014031143B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IB2012001446 2012-06-14
IBPCT/IB2012/001446 2012-06-14
PCT/EP2013/062405 WO2013186374A1 (en) 2012-06-14 2013-06-14 Method for quantifying immune cells in tumoral tissues and its applications

Publications (3)

Publication Number Publication Date
BR112014031143A2 BR112014031143A2 (pt) 2017-08-08
BR112014031143B1 BR112014031143B1 (pt) 2022-03-03
BR112014031143B8 true BR112014031143B8 (pt) 2022-11-22

Family

ID=46881097

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014031143A BR112014031143B8 (pt) 2012-06-14 2013-06-14 Método para quantificar células imunes em tecidos tumorais e suas aplicações

Country Status (13)

Country Link
US (1) US10317408B2 (pt)
EP (1) EP2861986B1 (pt)
JP (1) JP6449765B2 (pt)
KR (1) KR102179848B1 (pt)
CN (1) CN104541167B (pt)
AU (1) AU2013276470B2 (pt)
BR (1) BR112014031143B8 (pt)
CA (1) CA2873278C (pt)
DE (1) DE13731071T1 (pt)
ES (1) ES2902879T3 (pt)
IL (1) IL235738B (pt)
SG (1) SG11201408107RA (pt)
WO (1) WO2013186374A1 (pt)

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EP3111221B2 (en) 2014-02-24 2022-01-19 Ventana Medical Systems, Inc. Methods, kits, and systems for scoring the immune response to cancer by simultaneous detection of cd3, cd8, cd20 and foxp3.
WO2016034655A2 (en) * 2014-09-03 2016-03-10 Ventana Medical Systems, Inc. Systems and methods for generating fields of view
US11275080B2 (en) 2014-11-05 2022-03-15 The Regents Of The University Of California Methods for stratifying non-responders to therapies that block PD1/PDL1 axis
WO2016118832A1 (en) 2015-01-22 2016-07-28 University Of Massachusetts Cancer immunotherapy
US10705088B2 (en) * 2015-10-23 2020-07-07 Novartis Ag Method of scoring a sample comprising tumor tissue
PL3455631T3 (pl) * 2016-05-09 2021-04-06 INSERM (Institut National de la Santé et de la Recherche Médicale) Sposoby klasyfikowania pacjentów z nowotworem litym
US11315240B2 (en) * 2016-05-18 2022-04-26 Hoffmann-La Roche Inc. System and method of tumor classification
EP3532984B1 (en) * 2016-10-27 2024-05-08 Koninklijke Philips N.V. Apparatus for determining cellular composition information in one or more tissue samples
US11459568B2 (en) 2016-10-31 2022-10-04 University Of Massachusetts Targeting microRNA-101-3p in cancer therapy
CN106846310A (zh) * 2017-01-19 2017-06-13 宁波江丰生物信息技术有限公司 一种基于免疫组化技术的病理辅助分析方法
WO2019020556A1 (en) * 2017-07-24 2019-01-31 Ventana Medical Systems, Inc. METHODS AND SYSTEMS FOR ASSESSING INFILTRAT OF IMMUNE CELLS IN TUMOR SAMPLES
US11814623B2 (en) 2018-01-30 2023-11-14 University Of Massachusetts Methods of treating a wound using epigenetic regulation
JP7411667B2 (ja) * 2019-02-05 2024-01-11 ヴェンタナ メディカル システムズ， インク． ステージｉｖの結腸直腸がんにおける免疫細胞浸潤の評価のための方法及びシステム
WO2020245155A1 (en) 2019-06-03 2020-12-10 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for modulating a treatment regimen
CN114556480A (zh) 2019-11-07 2022-05-27 欧森纳治疗股份有限公司 肿瘤微环境的分类
CN111551546B (zh) * 2020-06-01 2023-01-03 南京瑟斯检测科技有限公司 一种基于光学微腔结构超材料的免疫组化便捷检测方法
EP4172621A1 (en) 2020-06-30 2023-05-03 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for predicting the risk of recurrence and/or death of patients suffering from a solid cancer after preoperative adjuvant therapies
CN115843335A (zh) 2020-06-30 2023-03-24 国家医疗保健研究所 用于预测患有实体癌的患者在术前辅助治疗和根治性手术后复发和/或死亡风险的方法
CN113092202A (zh) * 2021-03-25 2021-07-09 上海福君基因生物科技有限公司 一种胃癌预后预测装置
CN113515077A (zh) * 2021-04-23 2021-10-19 重庆德方信息技术有限公司 人体细胞染色过程监控系统及方法
WO2023018085A1 (ko) * 2021-08-10 2023-02-16 주식회사 루닛 병리 슬라이드 이미지와 관련된 정보를 출력하는 방법 및 장치
WO2023175366A1 (en) 2022-03-17 2023-09-21 Veracyte Methods for predicting response to an immunotherapeutic treatment in a patient with a cancer
CN116646088B (zh) * 2023-07-27 2023-12-01 广东省人民医院 一种预测方法、装置、设备及介质

Family Cites Families (5)

* Cited by examiner, † Cited by third party
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US6215892B1 (en) * 1995-11-30 2001-04-10 Chromavision Medical Systems, Inc. Method and apparatus for automated image analysis of biological specimens
EP1777523A1 (en) * 2005-10-19 2007-04-25 INSERM (Institut National de la Santé et de la Recherche Médicale) An in vitro method for the prognosis of progression of a cancer and of the outcome in a patient and means for performing said method
AU2006333078B2 (en) * 2005-12-19 2012-03-01 Ventana Medical Systems, Inc. Automated lean methods in anatomical pathology
CN102165489B (zh) * 2008-09-16 2015-11-25 赫斯托克斯公司 生物标志物表达的可再现量化
FR2964744B1 (fr) * 2010-09-10 2015-04-03 Univ Versailles St Quentin En Yvelines Test pronostic de l'evolution d'une tumeur solide par analyse d'images

Also Published As

Publication number Publication date
KR20150023760A (ko) 2015-03-05
EP2861986A1 (en) 2015-04-22
CA2873278C (en) 2021-10-26
ES2902879T3 (es) 2022-03-30
JP2015525349A (ja) 2015-09-03
WO2013186374A1 (en) 2013-12-19
US10317408B2 (en) 2019-06-11
IL235738A0 (en) 2015-01-29
CN104541167A (zh) 2015-04-22
BR112014031143A2 (pt) 2017-08-08
EP2861986B1 (en) 2021-11-10
AU2013276470B2 (en) 2018-06-07
DE13731071T1 (de) 2016-08-18
US20150153349A1 (en) 2015-06-04
IL235738B (en) 2019-02-28
CN104541167B (zh) 2018-05-01
AU2013276470A1 (en) 2014-11-27
CA2873278A1 (en) 2013-12-19
BR112014031143B1 (pt) 2022-03-03
KR102179848B1 (ko) 2020-11-17
SG11201408107RA (en) 2015-01-29
JP6449765B2 (ja) 2019-01-09

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
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Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

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B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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Owner name: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) (FR) ; ASSISTANCE PUBLIQUE HOPITAUX DE PARIS (FR) ; UNIVERSITE DE PARIS (FR)

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