BR112014031143B8 - Método para quantificar células imunes em tecidos tumorais e suas aplicações - Google Patents

Método para quantificar células imunes em tecidos tumorais e suas aplicações

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Publication number
BR112014031143B8
BR112014031143B8 BR112014031143A BR112014031143A BR112014031143B8 BR 112014031143 B8 BR112014031143 B8 BR 112014031143B8 BR 112014031143 A BR112014031143 A BR 112014031143A BR 112014031143 A BR112014031143 A BR 112014031143A BR 112014031143 B8 BR112014031143 B8 BR 112014031143B8
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BR
Brazil
Prior art keywords
immune cells
tumor
tumor tissues
applications
analyzed
Prior art date
Application number
BR112014031143A
Other languages
English (en)
Other versions
BR112014031143B1 (pt
BR112014031143A2 (pt
Inventor
Milecnik Bernhard
Galon Jérôme
Pagès Franck
Original Assignee
Univ Paris Descartes
Inst Nat Sante Rech Med
Hopitaux Paris Assist Publique
Univ Paris
Univ Paris Cite
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Application filed by Univ Paris Descartes, Inst Nat Sante Rech Med, Hopitaux Paris Assist Publique, Univ Paris, Univ Paris Cite filed Critical Univ Paris Descartes
Publication of BR112014031143A2 publication Critical patent/BR112014031143A2/pt
Publication of BR112014031143B1 publication Critical patent/BR112014031143B1/pt
Publication of BR112014031143B8 publication Critical patent/BR112014031143B8/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MÉTODO PARA QUANTIFICAR CÉLULAS IMUNES EM TECIDOS TUMORAIS E SUAS APLICAÇÕES. Um método para avaliação de um número ou a densidade de células imunológicas em tecidos tumorais compreendendo as etapas que consistem em: a. fornecer uma ou mais lâminas imunocoradas de secção de tecido obtidas por um sistema de coloração de lâminas automatizado usando anticorpos que se ligam especificamente a antígenos (marcadores) expressos pelas células imunológicas. b. proceder à digitalização das lâminas da etapa a. pela captura de imagem alta resolução, pelo qual uma imagem digital de alta definição (4,6 mm / pixel ou melhor) da lâmina a ser analisada é obtida, c. detectar a porção de corte de tecido sobre a imagem digital d. analisar a porção da seção de tecido para a definição (i) do tumor (TC) e (ii) da margem invasiva do tumor (IM), e. fornecer uma grade de referência de tamanho com unidades uniformemente distribuídas tendo uma mesma superfície, a dita grade sendo adaptada ao tamanho do tumor a ser analisado, e1. verificar a qualidade da immunocoloração, f. detectar e quantificar as células coradas de cada unidade em que o número ou a densidade de células imunológicas coradas de cada unidade é avaliada.
BR112014031143A 2012-06-14 2013-06-14 Método para quantificar células imunes em tecidos tumorais e suas aplicações BR112014031143B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IBPCT/IB2012/001446 2012-06-14
IB2012001446 2012-06-14
PCT/EP2013/062405 WO2013186374A1 (en) 2012-06-14 2013-06-14 Method for quantifying immune cells in tumoral tissues and its applications

Publications (3)

Publication Number Publication Date
BR112014031143A2 BR112014031143A2 (pt) 2017-08-08
BR112014031143B1 BR112014031143B1 (pt) 2022-03-03
BR112014031143B8 true BR112014031143B8 (pt) 2022-11-22

Family

ID=46881097

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014031143A BR112014031143B8 (pt) 2012-06-14 2013-06-14 Método para quantificar células imunes em tecidos tumorais e suas aplicações

Country Status (13)

Country Link
US (1) US10317408B2 (pt)
EP (1) EP2861986B1 (pt)
JP (1) JP6449765B2 (pt)
KR (1) KR102179848B1 (pt)
CN (1) CN104541167B (pt)
AU (1) AU2013276470B2 (pt)
BR (1) BR112014031143B8 (pt)
CA (1) CA2873278C (pt)
DE (1) DE13731071T1 (pt)
ES (1) ES2902879T3 (pt)
IL (1) IL235738B (pt)
SG (1) SG11201408107RA (pt)
WO (1) WO2013186374A1 (pt)

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JP6605506B2 (ja) 2014-02-24 2019-11-13 ヴェンタナ メディカル システムズ， インク． ＣＤ３、ＣＤ８、ＣＤ２０及びＦｏｘＰ３の同時検出によりがんに対する免疫応答をスコア化するための方法、キット、及びシステム
JP6802153B2 (ja) * 2014-09-03 2020-12-16 ヴェンタナ メディカル システムズ， インク． 免疫スコアを計算するためのシステム及び方法
US11275080B2 (en) 2014-11-05 2022-03-15 The Regents Of The University Of California Methods for stratifying non-responders to therapies that block PD1/PDL1 axis
US10034901B2 (en) 2015-01-22 2018-07-31 University Of Massachusetts Cancer immunotherapy
US10705088B2 (en) * 2015-10-23 2020-07-07 Novartis Ag Method of scoring a sample comprising tumor tissue
MX2018013744A (es) * 2016-05-09 2019-08-16 Inst Nat Sante Rech Med Metodos para clasificar pacientes con un cancer solido.
EP3459046B1 (en) * 2016-05-18 2021-06-16 F. Hoffmann-La Roche AG Tumor proximity measure
CN110168561B (zh) * 2016-10-27 2023-06-16 皇家飞利浦有限公司 用于确定一个或多个组织样本中的细胞组成信息的装置
EP3532638A4 (en) 2016-10-31 2020-07-29 University of Massachusetts TARGETING MICROARN-101-3 P IN CARCINOTHERAPY
CN106846310A (zh) * 2017-01-19 2017-06-13 宁波江丰生物信息技术有限公司 一种基于免疫组化技术的病理辅助分析方法
WO2019020556A1 (en) * 2017-07-24 2019-01-31 Ventana Medical Systems, Inc. METHODS AND SYSTEMS FOR ASSESSING INFILTRAT OF IMMUNE CELLS IN TUMOR SAMPLES
US11814623B2 (en) 2018-01-30 2023-11-14 University Of Massachusetts Methods of treating a wound using epigenetic regulation
WO2020161125A1 (en) * 2019-02-05 2020-08-13 Ventana Medical Systems, Inc. Methods and systems for evaluation of immune cell infiltrate in stage iv colorectal cancer
WO2020245155A1 (en) 2019-06-03 2020-12-10 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods for modulating a treatment regimen
WO2021092071A1 (en) 2019-11-07 2021-05-14 Oncxerna Therapeutics, Inc. Classification of tumor microenvironments
CN111551546B (zh) * 2020-06-01 2023-01-03 南京瑟斯检测科技有限公司 一种基于光学微腔结构超材料的免疫组化便捷检测方法
JP2023531290A (ja) 2020-06-30 2023-07-21 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 術前補助療法及び根治手術後の固形がんを患っている患者の再発及び／又は死亡のリスクを予測するための方法
JP2023531305A (ja) 2020-06-30 2023-07-21 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 術前補助療法後の固形癌患者の再発及び／又は死亡のリスクを予測するための方法。
CN113092202A (zh) * 2021-03-25 2021-07-09 上海福君基因生物科技有限公司 一种胃癌预后预测装置
CN113515077A (zh) * 2021-04-23 2021-10-19 重庆德方信息技术有限公司 人体细胞染色过程监控系统及方法
EP4369297A1 (en) 2021-08-10 2024-05-15 Lunit Inc. Method and device for outputting information related to pathological slide image
WO2023175366A1 (en) 2022-03-17 2023-09-21 Veracyte Methods for predicting response to an immunotherapeutic treatment in a patient with a cancer
CN116646088B (zh) * 2023-07-27 2023-12-01 广东省人民医院 一种预测方法、装置、设备及介质

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1300713A3 (en) * 1995-11-30 2004-11-03 Chromavision Medical Systems, Inc. Method and apparatus for automated image analysis of biological specimens
EP1777523A1 (en) * 2005-10-19 2007-04-25 INSERM (Institut National de la Santé et de la Recherche Médicale) An in vitro method for the prognosis of progression of a cancer and of the outcome in a patient and means for performing said method
AU2006333078B2 (en) * 2005-12-19 2012-03-01 Ventana Medical Systems, Inc. Automated lean methods in anatomical pathology
EP2335221B8 (en) * 2008-09-16 2016-05-25 Novartis AG Reproducible quantification of biomarker expression
FR2964744B1 (fr) * 2010-09-10 2015-04-03 Univ Versailles St Quentin En Yvelines Test pronostic de l'evolution d'une tumeur solide par analyse d'images

Also Published As

Publication number Publication date
CN104541167A (zh) 2015-04-22
EP2861986B1 (en) 2021-11-10
JP6449765B2 (ja) 2019-01-09
AU2013276470B2 (en) 2018-06-07
WO2013186374A1 (en) 2013-12-19
KR102179848B1 (ko) 2020-11-17
ES2902879T3 (es) 2022-03-30
SG11201408107RA (en) 2015-01-29
BR112014031143B1 (pt) 2022-03-03
DE13731071T1 (de) 2016-08-18
AU2013276470A1 (en) 2014-11-27
CA2873278C (en) 2021-10-26
US20150153349A1 (en) 2015-06-04
CN104541167B (zh) 2018-05-01
JP2015525349A (ja) 2015-09-03
US10317408B2 (en) 2019-06-11
EP2861986A1 (en) 2015-04-22
CA2873278A1 (en) 2013-12-19
BR112014031143A2 (pt) 2017-08-08
KR20150023760A (ko) 2015-03-05
IL235738B (en) 2019-02-28
IL235738A0 (en) 2015-01-29

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
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Owner name: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE) (FR) ; ASSISTANCE PUBLIQUE HOPITAUX DE PARIS (FR) ; UNIVERSITE DE PARIS (FR)

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