CN104541167B - 用于定量肿瘤组织中的免疫细胞的方法及其应用 - Google Patents
用于定量肿瘤组织中的免疫细胞的方法及其应用 Download PDFInfo
- Publication number
- CN104541167B CN104541167B CN201380030888.7A CN201380030888A CN104541167B CN 104541167 B CN104541167 B CN 104541167B CN 201380030888 A CN201380030888 A CN 201380030888A CN 104541167 B CN104541167 B CN 104541167B
- Authority
- CN
- China
- Prior art keywords
- cell
- tumour
- unit
- value
- density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 163
- 238000000034 method Methods 0.000 title claims abstract description 75
- 238000010186 staining Methods 0.000 claims abstract description 60
- 239000011521 glass Substances 0.000 claims abstract description 46
- 239000003550 marker Substances 0.000 claims abstract description 32
- 238000004043 dyeing Methods 0.000 claims abstract description 26
- 238000012744 immunostaining Methods 0.000 claims abstract description 24
- 230000009870 specific binding Effects 0.000 claims abstract description 3
- 238000004040 coloring Methods 0.000 claims abstract 2
- 238000004458 analytical method Methods 0.000 claims description 25
- 238000001514 detection method Methods 0.000 claims description 18
- 102100030385 Granzyme B Human genes 0.000 claims description 17
- 101001009603 Homo sapiens Granzyme B Proteins 0.000 claims description 17
- 102100022005 B-lymphocyte antigen CD20 Human genes 0.000 claims description 16
- 101000897405 Homo sapiens B-lymphocyte antigen CD20 Proteins 0.000 claims description 16
- 239000000975 dye Substances 0.000 claims description 8
- 238000005259 measurement Methods 0.000 claims description 6
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 claims description 4
- -1 CD45RO Proteins 0.000 claims description 3
- 102000017420 CD3 protein, epsilon/gamma/delta subunit Human genes 0.000 claims 1
- 108050005493 CD3 protein, epsilon/gamma/delta subunit Proteins 0.000 claims 1
- 101000946843 Homo sapiens T-cell surface glycoprotein CD8 alpha chain Proteins 0.000 claims 1
- 102100034922 T-cell surface glycoprotein CD8 alpha chain Human genes 0.000 claims 1
- 238000012550 audit Methods 0.000 claims 1
- 239000000427 antigen Substances 0.000 abstract description 4
- 102000036639 antigens Human genes 0.000 abstract description 4
- 108091007433 antigens Proteins 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 128
- 210000001519 tissue Anatomy 0.000 description 66
- 239000000523 sample Substances 0.000 description 42
- 201000011510 cancer Diseases 0.000 description 25
- 239000000090 biomarker Substances 0.000 description 18
- 230000004083 survival effect Effects 0.000 description 13
- 238000004393 prognosis Methods 0.000 description 12
- 230000004044 response Effects 0.000 description 10
- 238000010586 diagram Methods 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 210000001744 T-lymphocyte Anatomy 0.000 description 6
- 206010009944 Colon cancer Diseases 0.000 description 5
- 238000011319 anticancer therapy Methods 0.000 description 5
- 238000013459 approach Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 241000208340 Araliaceae Species 0.000 description 4
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 4
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 4
- 235000003140 Panax quinquefolius Nutrition 0.000 description 4
- 238000001574 biopsy Methods 0.000 description 4
- 235000008434 ginseng Nutrition 0.000 description 4
- 238000001325 log-rank test Methods 0.000 description 4
- 238000001815 biotherapy Methods 0.000 description 3
- 238000002512 chemotherapy Methods 0.000 description 3
- 230000028993 immune response Effects 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 230000003053 immunization Effects 0.000 description 3
- 230000002055 immunohistochemical effect Effects 0.000 description 3
- 238000003364 immunohistochemistry Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000002493 microarray Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 239000012188 paraffin wax Substances 0.000 description 3
- 238000001959 radiotherapy Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 210000001266 CD8-positive T-lymphocyte Anatomy 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 206010027476 Metastases Diseases 0.000 description 2
- 238000011532 immunohistochemical staining Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 241000872198 Serjania polyphylla Species 0.000 description 1
- 240000002825 Solanum vestissimum Species 0.000 description 1
- 235000018259 Solanum vestissimum Nutrition 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009830 antibody antigen interaction Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003822 epoxy resin Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 201000005202 lung cancer Diseases 0.000 description 1
- 208000020816 lung neoplasm Diseases 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 210000003071 memory t lymphocyte Anatomy 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920000647 polyepoxide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57492—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds localized on the membrane of tumor or cancer cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5047—Cells of the immune system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Abstract
Description
标准的组合 | A组 | B组 | C组 |
C1 | 7 | 4 | 3 |
C2 | 7 | 5 | 3 |
C3 | 10 | 4 | 3 |
C4 | 10 | 5 | 3 |
C5 | 6 | 4 | 3 |
C6 | 6 | 5 | 3 |
C7 | 9 | 4 | 3 |
C8 | 9 | 5 | 3 |
C9 | 4 | 4 | 3 |
C10 | 4 | 5 | 3 |
C11 | 3 | 4 | 3 |
C12 | 3 | 5 | 3 |
C13 | 5 | 4 | 3 |
C14 | 5 | 5 | 3 |
C15 | 7 | 4 | 2 |
Claims (16)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IB2012001446 | 2012-06-14 | ||
IBPCT/IB2012/001446 | 2012-06-14 | ||
PCT/EP2013/062405 WO2013186374A1 (en) | 2012-06-14 | 2013-06-14 | Method for quantifying immune cells in tumoral tissues and its applications |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104541167A CN104541167A (zh) | 2015-04-22 |
CN104541167B true CN104541167B (zh) | 2018-05-01 |
Family
ID=46881097
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201380030888.7A Active CN104541167B (zh) | 2012-06-14 | 2013-06-14 | 用于定量肿瘤组织中的免疫细胞的方法及其应用 |
Country Status (13)
Country | Link |
---|---|
US (1) | US10317408B2 (zh) |
EP (1) | EP2861986B1 (zh) |
JP (1) | JP6449765B2 (zh) |
KR (1) | KR102179848B1 (zh) |
CN (1) | CN104541167B (zh) |
AU (1) | AU2013276470B2 (zh) |
BR (1) | BR112014031143B8 (zh) |
CA (1) | CA2873278C (zh) |
DE (1) | DE13731071T1 (zh) |
ES (1) | ES2902879T3 (zh) |
IL (1) | IL235738B (zh) |
SG (1) | SG11201408107RA (zh) |
WO (1) | WO2013186374A1 (zh) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2701092T5 (es) | 2014-02-24 | 2022-05-12 | Ventana Med Syst Inc | Procedimientos, kits y sistemas para puntuar la respuesta inmunitaria al cáncer por detección simultánea de CD3, CD8, CD20 y FOXP3 |
EP3195191B1 (en) * | 2014-09-03 | 2020-10-21 | Ventana Medical Systems, Inc. | Systems and methods for calculating immune scores |
WO2016073760A1 (en) * | 2014-11-05 | 2016-05-12 | The Regents Of The University Of California | Methods for stratifying non-responders to therapies that block pd1/pdl1 axis |
CA2973878A1 (en) | 2015-01-22 | 2016-07-28 | University Of Massachusetts | Cancer immunotherapy |
CN108136216B (zh) * | 2015-10-23 | 2021-08-24 | 诺华股份有限公司 | 对包含肿瘤组织的样品评分的方法 |
MX2018013744A (es) * | 2016-05-09 | 2019-08-16 | Inst Nat Sante Rech Med | Metodos para clasificar pacientes con un cancer solido. |
US11315240B2 (en) * | 2016-05-18 | 2022-04-26 | Hoffmann-La Roche Inc. | System and method of tumor classification |
US11048911B2 (en) * | 2016-10-27 | 2021-06-29 | Koninklijke Philips N.V. | Apparatus for determining cellular composition information in one or more tissue samples |
EP3532638A4 (en) | 2016-10-31 | 2020-07-29 | University of Massachusetts | TARGETING MICROARN-101-3 P IN CARCINOTHERAPY |
CN106846310A (zh) * | 2017-01-19 | 2017-06-13 | 宁波江丰生物信息技术有限公司 | 一种基于免疫组化技术的病理辅助分析方法 |
WO2019020556A1 (en) * | 2017-07-24 | 2019-01-31 | Ventana Medical Systems, Inc. | METHODS AND SYSTEMS FOR ASSESSING INFILTRAT OF IMMUNE CELLS IN TUMOR SAMPLES |
US11814623B2 (en) | 2018-01-30 | 2023-11-14 | University Of Massachusetts | Methods of treating a wound using epigenetic regulation |
WO2020161125A1 (en) * | 2019-02-05 | 2020-08-13 | Ventana Medical Systems, Inc. | Methods and systems for evaluation of immune cell infiltrate in stage iv colorectal cancer |
JP2022536075A (ja) | 2019-06-03 | 2022-08-12 | アンセルム(アンスティチュート・ナシオナル・ドゥ・ラ・サンテ・エ・ドゥ・ラ・ルシェルシュ・メディカル) | 処置レジメンを調節する方法 |
TW202132573A (zh) | 2019-11-07 | 2021-09-01 | 美商昂克賽納醫療公司 | 腫瘤微環境之分類 |
CN111551546B (zh) * | 2020-06-01 | 2023-01-03 | 南京瑟斯检测科技有限公司 | 一种基于光学微腔结构超材料的免疫组化便捷检测方法 |
EP4172628A1 (en) | 2020-06-30 | 2023-05-03 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for predicting the risk of recurrence and/or death of patients suffering from a solid cancer after preoperative adjuvant therapy and radical surgery |
JP2023531305A (ja) | 2020-06-30 | 2023-07-21 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | 術前補助療法後の固形癌患者の再発及び/又は死亡のリスクを予測するための方法。 |
CN113092202A (zh) * | 2021-03-25 | 2021-07-09 | 上海福君基因生物科技有限公司 | 一种胃癌预后预测装置 |
CN113515077A (zh) * | 2021-04-23 | 2021-10-19 | 重庆德方信息技术有限公司 | 人体细胞染色过程监控系统及方法 |
WO2023018085A1 (ko) | 2021-08-10 | 2023-02-16 | 주식회사 루닛 | 병리 슬라이드 이미지와 관련된 정보를 출력하는 방법 및 장치 |
WO2023175366A1 (en) | 2022-03-17 | 2023-09-21 | Veracyte | Methods for predicting response to an immunotherapeutic treatment in a patient with a cancer |
CN116646088B (zh) * | 2023-07-27 | 2023-12-01 | 广东省人民医院 | 一种预测方法、装置、设备及介质 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6215892B1 (en) * | 1995-11-30 | 2001-04-10 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
EP1777523A1 (en) * | 2005-10-19 | 2007-04-25 | INSERM (Institut National de la Santé et de la Recherche Médicale) | An in vitro method for the prognosis of progression of a cancer and of the outcome in a patient and means for performing said method |
ES2593930T3 (es) * | 2005-12-19 | 2016-12-14 | Ventana Medical Systems, Inc. | Impresión automatizada de etiquetas para portaobjetos de especímenes de tejido |
JP5551702B2 (ja) * | 2008-09-16 | 2014-07-16 | ヒストロックス,インコーポレイテッド. | バイオマーカー発現の再現性のある定量 |
FR2964744B1 (fr) * | 2010-09-10 | 2015-04-03 | Univ Versailles St Quentin En Yvelines | Test pronostic de l'evolution d'une tumeur solide par analyse d'images |
-
2013
- 2013-06-14 ES ES13731071T patent/ES2902879T3/es active Active
- 2013-06-14 CN CN201380030888.7A patent/CN104541167B/zh active Active
- 2013-06-14 BR BR112014031143A patent/BR112014031143B8/pt active IP Right Grant
- 2013-06-14 EP EP13731071.0A patent/EP2861986B1/en active Active
- 2013-06-14 KR KR1020157000987A patent/KR102179848B1/ko active IP Right Grant
- 2013-06-14 WO PCT/EP2013/062405 patent/WO2013186374A1/en active Application Filing
- 2013-06-14 SG SG11201408107RA patent/SG11201408107RA/en unknown
- 2013-06-14 JP JP2015516637A patent/JP6449765B2/ja active Active
- 2013-06-14 AU AU2013276470A patent/AU2013276470B2/en active Active
- 2013-06-14 DE DE13731071.0T patent/DE13731071T1/de active Pending
- 2013-06-14 US US14/407,698 patent/US10317408B2/en active Active
- 2013-06-14 CA CA2873278A patent/CA2873278C/en active Active
-
2014
- 2014-11-17 IL IL235738A patent/IL235738B/en active IP Right Grant
Non-Patent Citations (5)
Title |
---|
Localization and Density of Immune Cells in the Invasive Margin of Human Colorectal Cancer Liver Metastases Are Prognostic for Response to Chemotherapy;Niels Halama;《Cancer research》;20110901;第17卷;摘要,第5671页右栏第4段,第5672页左栏第2-3段、右栏第4段,第5673页,第5675页左栏第2段,图2-4 * |
The local immunological microenvironment in colorectal cancer as a prognostic factor for treatment decisions in the clinic;Niels Halama;《OncoImmunology》;20120101;第1卷(第1期);图1、图2,第63页第1栏第36-56行,第63页第2栏第15-27行,第64页第2栏第11-22行,第64页第3栏第3-13行 * |
免疫组化技术标准化的探讨;王文勇;《细胞与分子免疫学杂志》;20110831;第27卷(第8期);第928页第6-7节 * |
全自动免疫组化仪的应用及影响其染色质量的因素探讨;虞杰;《泰州职业技术学院学报》;20111231;第11卷(第6期);摘要,第70页右栏第1段 * |
组织芯片在免疫组化染色阳性对照中的应用;王翠芝 等;《临床与实验病理学杂志》;20060831;第22卷(第4期);第481-第484页 * |
Also Published As
Publication number | Publication date |
---|---|
CA2873278C (en) | 2021-10-26 |
BR112014031143B1 (pt) | 2022-03-03 |
KR102179848B1 (ko) | 2020-11-17 |
BR112014031143A2 (pt) | 2017-08-08 |
ES2902879T3 (es) | 2022-03-30 |
WO2013186374A1 (en) | 2013-12-19 |
US20150153349A1 (en) | 2015-06-04 |
AU2013276470A1 (en) | 2014-11-27 |
AU2013276470B2 (en) | 2018-06-07 |
US10317408B2 (en) | 2019-06-11 |
JP6449765B2 (ja) | 2019-01-09 |
CA2873278A1 (en) | 2013-12-19 |
EP2861986A1 (en) | 2015-04-22 |
DE13731071T1 (de) | 2016-08-18 |
IL235738A0 (en) | 2015-01-29 |
EP2861986B1 (en) | 2021-11-10 |
SG11201408107RA (en) | 2015-01-29 |
CN104541167A (zh) | 2015-04-22 |
IL235738B (en) | 2019-02-28 |
KR20150023760A (ko) | 2015-03-05 |
JP2015525349A (ja) | 2015-09-03 |
BR112014031143B8 (pt) | 2022-11-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104541167B (zh) | 用于定量肿瘤组织中的免疫细胞的方法及其应用 | |
US20220051804A1 (en) | Image Analysis for Breast Cancer Prognosis | |
US9298968B1 (en) | Digital image analysis of inflammatory cells and mediators of inflammation | |
Lloyd et al. | Using image analysis as a tool for assessment of prognostic and predictive biomarkers for breast cancer: How reliable is it? | |
EP3117259B1 (en) | Assessment of staining quality | |
JP5866362B2 (ja) | 画像解析による固形腫瘍進行の予後検査 | |
US20220389523A1 (en) | Image acquisition methods for simultaneously detecting genetic rearrangement and nuclear morphology | |
Balkenhol et al. | Optimized tumour infiltrating lymphocyte assessment for triple negative breast cancer prognostics | |
WO2017171720A1 (en) | Digital image analysis of inflammatory cells and mediators of inflammation | |
Jing et al. | Validity and reliability of Ki-67 assessment in oestrogen receptor positive breast cancer | |
EP4120187A1 (en) | A method for measuring a prognostic marker in prostate cancer | |
CN114022472B (zh) | 一种肿瘤浸润淋巴细胞分析方法、装置和存储介质 | |
CN115602313B (zh) | 用于疾病疗效与生存预后预测的生物标志物及其用途 | |
Besusparis | The assessment of the breast cancer proliferation rate and its intratumor heterogeneity using digital immunohistochemistry methods |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP01 | Change in the name or title of a patent holder | ||
CP01 | Change in the name or title of a patent holder |
Address after: Paris France Patentee after: INSERM (INSTITUT NATIONAL DE LA SANTe ET DE LA RECHERCHE MeDICALE) Patentee after: University of Western dais, Paris Patentee after: ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS Address before: Paris France Patentee before: INSERM (INSTITUT NATIONAL DE LA SANTe ET DE LA RECHERCHE MeDICALE) Patentee before: University of Paris Patentee before: ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230103 Address after: Paris France Patentee after: INSERM (INSTITUT NATIONAL DE LA SANTe ET DE LA RECHERCHE MeDICALE) Patentee after: University of Paris Patentee after: ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS Address before: Paris France Patentee before: INSERM (INSTITUT NATIONAL DE LA SANTe ET DE LA RECHERCHE MeDICALE) Patentee before: UNIVERSITE PARIS DESCARTES Patentee before: ASSISTANCE PUBLIQUE - HOPITAUX DE PARIS |