BR112014021326A2 - célula para a expressão de protéina, método para reduzir a atividade de amidação de peptídeo em uma determinada célula, uso de uma célula - Google Patents
célula para a expressão de protéina, método para reduzir a atividade de amidação de peptídeo em uma determinada célula, uso de uma célulaInfo
- Publication number
- BR112014021326A2 BR112014021326A2 BR112014021326A BR112014021326A BR112014021326A2 BR 112014021326 A2 BR112014021326 A2 BR 112014021326A2 BR 112014021326 A BR112014021326 A BR 112014021326A BR 112014021326 A BR112014021326 A BR 112014021326A BR 112014021326 A2 BR112014021326 A2 BR 112014021326A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell
- protein expression
- peptide amidation
- amidation activity
- reducing peptide
- Prior art date
Links
- 230000000694 effects Effects 0.000 title abstract 4
- 230000006228 peptide amidation Effects 0.000 title abstract 4
- 238000010635 peptide amidation reaction Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
- C12Y114/17003—Peptidylglycine monooxygenase (1.14.17.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12164264 | 2012-04-16 | ||
| PCT/EP2013/057866 WO2013156458A1 (en) | 2012-04-16 | 2013-04-16 | Reduction of formation of amidated amino acids in cell lines for protein expression |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112014021326A2 true BR112014021326A2 (pt) | 2017-07-04 |
Family
ID=48095869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014021326A BR112014021326A2 (pt) | 2012-04-16 | 2013-04-16 | célula para a expressão de protéina, método para reduzir a atividade de amidação de peptídeo em uma determinada célula, uso de uma célula |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9637769B2 (enExample) |
| EP (1) | EP2839003B1 (enExample) |
| JP (2) | JP6438387B2 (enExample) |
| KR (2) | KR20140145196A (enExample) |
| CN (1) | CN104903447A (enExample) |
| AU (1) | AU2013248356B2 (enExample) |
| BR (1) | BR112014021326A2 (enExample) |
| CA (1) | CA2864466C (enExample) |
| ES (1) | ES2688727T3 (enExample) |
| IN (1) | IN2014DN09585A (enExample) |
| SI (1) | SI2839003T1 (enExample) |
| WO (1) | WO2013156458A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2688727T3 (es) | 2012-04-16 | 2018-11-06 | Lek Pharmaceuticals D.D. | Reducción de la formación de aminoácidos amidados en líneas celulares para la expresión de proteína |
| SG11201601262QA (en) | 2013-08-20 | 2016-03-30 | Lek Pharmaceuticals | CELL CULTURE MEDIUM AND PROCESS FOR CONTROLLING α-AMIDATION AND/OR C-TERMINAL AMINO ACID CLEAVAGE OF POLYPEPTIDES |
| US10913985B2 (en) | 2014-11-12 | 2021-02-09 | Lek Pharmaceuticals D.D. | Predicting productivity in early cell line development |
| CN108660155A (zh) * | 2017-03-31 | 2018-10-16 | 胡育诚 | 过表达靶蛋白的系统和方法 |
| JP7453151B2 (ja) * | 2018-11-02 | 2024-03-19 | 協和キリン株式会社 | 液体培地の調製方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006084111A2 (en) * | 2005-02-04 | 2006-08-10 | Glaxo Group Limited | Optimization of heterologous polypeptide expression |
| US20080227134A9 (en) * | 2005-09-16 | 2008-09-18 | Unigene Laboratories Inc. | Glyoxylate assays and their use of inden tifying natural amidated compounds |
| EP2450375A1 (en) * | 2010-11-09 | 2012-05-09 | Sandoz Gmbh | Cell culture medium and process for protein expression, said medium and process comprising a PAM inhibitor |
| ES2688727T3 (es) | 2012-04-16 | 2018-11-06 | Lek Pharmaceuticals D.D. | Reducción de la formación de aminoácidos amidados en líneas celulares para la expresión de proteína |
-
2013
- 2013-04-16 ES ES13716301.0T patent/ES2688727T3/es active Active
- 2013-04-16 US US14/384,803 patent/US9637769B2/en active Active
- 2013-04-16 KR KR1020147031609A patent/KR20140145196A/ko not_active Ceased
- 2013-04-16 WO PCT/EP2013/057866 patent/WO2013156458A1/en not_active Ceased
- 2013-04-16 JP JP2015506206A patent/JP6438387B2/ja active Active
- 2013-04-16 EP EP13716301.0A patent/EP2839003B1/en active Active
- 2013-04-16 CA CA2864466A patent/CA2864466C/en not_active Expired - Fee Related
- 2013-04-16 AU AU2013248356A patent/AU2013248356B2/en not_active Ceased
- 2013-04-16 IN IN9585DEN2014 patent/IN2014DN09585A/en unknown
- 2013-04-16 BR BR112014021326A patent/BR112014021326A2/pt not_active Application Discontinuation
- 2013-04-16 SI SI201331158T patent/SI2839003T1/sl unknown
- 2013-04-16 KR KR1020177031195A patent/KR20170124618A/ko not_active Ceased
- 2013-04-16 CN CN201380020180.3A patent/CN104903447A/zh active Pending
-
2017
- 2017-08-18 JP JP2017157971A patent/JP2018068279A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013156458A1 (en) | 2013-10-24 |
| WO2013156458A8 (en) | 2014-11-20 |
| KR20140145196A (ko) | 2014-12-22 |
| AU2013248356A1 (en) | 2014-09-18 |
| EP2839003B1 (en) | 2018-06-27 |
| AU2013248356A2 (en) | 2014-09-25 |
| EP2839003A1 (en) | 2015-02-25 |
| CA2864466A1 (en) | 2013-10-24 |
| CN104903447A (zh) | 2015-09-09 |
| US20150079634A1 (en) | 2015-03-19 |
| JP6438387B2 (ja) | 2018-12-12 |
| AU2013248356B2 (en) | 2017-03-16 |
| US9637769B2 (en) | 2017-05-02 |
| CA2864466C (en) | 2021-03-02 |
| JP2015519884A (ja) | 2015-07-16 |
| ES2688727T3 (es) | 2018-11-06 |
| SI2839003T1 (sl) | 2018-10-30 |
| JP2018068279A (ja) | 2018-05-10 |
| IN2014DN09585A (enExample) | 2015-07-31 |
| KR20170124618A (ko) | 2017-11-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B06G | Technical and formal requirements: other requirements [chapter 6.7 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] |
Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL. |