CN104903447A - 在用于蛋白质表达的细胞系中减少酰胺化的氨基酸形成 - Google Patents

在用于蛋白质表达的细胞系中减少酰胺化的氨基酸形成 Download PDF

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Publication number
CN104903447A
CN104903447A CN201380020180.3A CN201380020180A CN104903447A CN 104903447 A CN104903447 A CN 104903447A CN 201380020180 A CN201380020180 A CN 201380020180A CN 104903447 A CN104903447 A CN 104903447A
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cell
gene
peptide
enzyme
cells
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CN201380020180.3A
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Chinese (zh)
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米哈埃拉·斯库利
多米尼克·戈赛尔
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Lek Pharmaceuticals dd
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Lek Pharmaceuticals dd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/17—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced ascorbate as one donor, and incorporation of one atom of oxygen (1.14.17)
    • C12Y114/17003—Peptidylglycine monooxygenase (1.14.17.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN201380020180.3A 2012-04-16 2013-04-16 在用于蛋白质表达的细胞系中减少酰胺化的氨基酸形成 Pending CN104903447A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12164264.9 2012-04-16
EP12164264 2012-04-16
PCT/EP2013/057866 WO2013156458A1 (en) 2012-04-16 2013-04-16 Reduction of formation of amidated amino acids in cell lines for protein expression

Publications (1)

Publication Number Publication Date
CN104903447A true CN104903447A (zh) 2015-09-09

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CN201380020180.3A Pending CN104903447A (zh) 2012-04-16 2013-04-16 在用于蛋白质表达的细胞系中减少酰胺化的氨基酸形成

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Country Link
US (1) US9637769B2 (enExample)
EP (1) EP2839003B1 (enExample)
JP (2) JP6438387B2 (enExample)
KR (2) KR20170124618A (enExample)
CN (1) CN104903447A (enExample)
AU (1) AU2013248356B2 (enExample)
BR (1) BR112014021326A2 (enExample)
CA (1) CA2864466C (enExample)
ES (1) ES2688727T3 (enExample)
IN (1) IN2014DN09585A (enExample)
SI (1) SI2839003T1 (enExample)
WO (1) WO2013156458A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108660155A (zh) * 2017-03-31 2018-10-16 胡育诚 过表达靶蛋白的系统和方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014021326A2 (pt) 2012-04-16 2017-07-04 Lek Pharmaceuticals célula para a expressão de protéina, método para reduzir a atividade de amidação de peptídeo em uma determinada célula, uso de uma célula
WO2015024977A1 (en) 2013-08-20 2015-02-26 Lek Pharmaceuticals D.D. CELL CULTURE MEDIUM AND PROCESS FOR CONTROLLING α-AMIDATION AND/OR C-TERMINAL AMINO ACID CLEAVAGE OF POLYPEPTIDES
SI3218478T1 (sl) 2014-11-12 2020-08-31 Lek Pharmaceuticals D.D. Predvidevanje produktivnosti zgodaj v razvoju celične linije
US20210403971A1 (en) * 2018-11-02 2021-12-30 Kyowa Kirin Co., Ltd. Method for preparing liquid medium

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006084111A2 (en) 2005-02-04 2006-08-10 Glaxo Group Limited Optimization of heterologous polypeptide expression
US20080227134A9 (en) 2005-09-16 2008-09-18 Unigene Laboratories Inc. Glyoxylate assays and their use of inden tifying natural amidated compounds
EP2450375A1 (en) * 2010-11-09 2012-05-09 Sandoz Gmbh Cell culture medium and process for protein expression, said medium and process comprising a PAM inhibitor
BR112014021326A2 (pt) 2012-04-16 2017-07-04 Lek Pharmaceuticals célula para a expressão de protéina, método para reduzir a atividade de amidação de peptídeo em uma determinada célula, uso de uma célula

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JOHNSON K等: "Cation exchange-HPLC and mass spectrometry reveal C-terminal amidation of an IgG1 heavy chain", 《ANALYTICAL BIOCHEMISTRY》 *
MAINS R E等: "Inhibition of peptide amidation by disulfirm and diethyldithiocarbamate", 《THE JOURNAL OF BIOLOGICAL CHEMISTRY》 *
TRACI A. CZYZYK，ET AL.: "Deletion of peptide amidation enzymatic activity leads to edema and embryonic lethality in the mouse", 《DEVELOPMENTAL BIOLOGY》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108660155A (zh) * 2017-03-31 2018-10-16 胡育诚 过表达靶蛋白的系统和方法

Also Published As

Publication number Publication date
CA2864466A1 (en) 2013-10-24
US20150079634A1 (en) 2015-03-19
JP6438387B2 (ja) 2018-12-12
AU2013248356A2 (en) 2014-09-25
BR112014021326A2 (pt) 2017-07-04
KR20170124618A (ko) 2017-11-10
ES2688727T3 (es) 2018-11-06
AU2013248356B2 (en) 2017-03-16
JP2015519884A (ja) 2015-07-16
SI2839003T1 (sl) 2018-10-30
JP2018068279A (ja) 2018-05-10
IN2014DN09585A (enExample) 2015-07-31
WO2013156458A1 (en) 2013-10-24
CA2864466C (en) 2021-03-02
AU2013248356A1 (en) 2014-09-18
KR20140145196A (ko) 2014-12-22
WO2013156458A8 (en) 2014-11-20
EP2839003A1 (en) 2015-02-25
US9637769B2 (en) 2017-05-02
EP2839003B1 (en) 2018-06-27

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