BR112014006741A2 - método para monitorização, diagnóstico e/ou prognóstico de lesão renal aguda no estágio inicial - Google Patents

método para monitorização, diagnóstico e/ou prognóstico de lesão renal aguda no estágio inicial

Info

Publication number
BR112014006741A2
BR112014006741A2 BR112014006741A BR112014006741A BR112014006741A2 BR 112014006741 A2 BR112014006741 A2 BR 112014006741A2 BR 112014006741 A BR112014006741 A BR 112014006741A BR 112014006741 A BR112014006741 A BR 112014006741A BR 112014006741 A2 BR112014006741 A2 BR 112014006741A2
Authority
BR
Brazil
Prior art keywords
kit
specific
kidney injury
urine sample
early
Prior art date
Application number
BR112014006741A
Other languages
English (en)
Inventor
Ernesto Irarrazabal Muñoz Carlos
Original Assignee
Univ Los Andes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=47913944&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112014006741(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Los Andes filed Critical Univ Los Andes
Publication of BR112014006741A2 publication Critical patent/BR112014006741A2/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/52—Assays involving cytokines
    • G01N2333/54—Interleukins [IL]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/8139—Cysteine protease (E.C. 3.4.22) inhibitors, e.g. cystatin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/34—Genitourinary disorders
    • G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

abstract the present invention relates to a method and a kit for monitoring, diagnosis, prognosis of acute kidney injury in early stage and determination of treatment in subjects suffering thereof. the method comprises the steps of a) providing a urine sample; b) enriching the urine sample in exosomes present in the urine sample using at least one step of immunopurification; c) detecting an acute kidney injury (aki) marker in the exosome. the invention also relates to a kit for determining the presence and/or level of a specific kidney injury marker, for simple and early determination of the onset of aki in a subject, the kit comprising means for enriching the urine sample in exosomes using at least one step of immunopurification, and means for detecting a predetermined kidney injury marker of a condition. the method and kit of the invention have the advantage to provide a specific, simple and early detection of kidney injury markers that is no possible with the standard methods applied until now. the method and kit of the invention also provide the advantage of identifying the specific structure that is injured in the kidney, since it uses specific antibodies for specific proteins present in specific structures of the kidney. --------------------------------------------------------------------------tradução do resumo resumo patente de invenção: "método para monitorização, diagnóstico e/ou prognóstico de lesão renal aguda no estágio inicial". a presente invenção se refere a um método e a um kit para mo-nitorização, diagnóstico, prognóstico de lesão renal aguda no estágio inicial e determinação de tratamento em sujeitos que sofram dela. o método com-preende as etapas de a) obtenção de uma amostra de urina; b) enriqueci-mento da amostra de urina em exossomos presentes na amostra de urina usando pelo menos uma etapa de imunopurificação; c) detecção de um mar-cador de lesão renal aguda (aki) no exossomo. a invenção também se refere a um kit para determinação da presença e/ou nível de um marcador de lesão renal específico, para determinação simples e precoce do início de aki em um sujeito, o kit compreendendo meios para enriquecimento da amostra de urina em exossomos usando pelo menos uma etapa de imunopurificação, e meios para detecção de um marcador de lesão renal predeterminado de uma condição. o método e o kit da invenção têm a vantagem de proporcionarem uma detecção específica, simples e precoce de marcadores de lesão renal, o que não era possível com os métodos padronizados aplicados até agora. o método e o kit da invenção também proporcionam a vantagem de identificar a estrutura específica que está lesionada no rim, pois usam anticorpos específicos para proteínas específicas presentes em estruturas específicas do rim.
BR112014006741A 2011-09-22 2011-09-22 método para monitorização, diagnóstico e/ou prognóstico de lesão renal aguda no estágio inicial BR112014006741A2 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2011/054187 WO2013041913A1 (en) 2011-09-22 2011-09-22 Method for monitoring, diagnosis and/or prognosis of acute kidney injury in early stage

Publications (1)

Publication Number Publication Date
BR112014006741A2 true BR112014006741A2 (pt) 2017-03-28

Family

ID=47913944

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014006741A BR112014006741A2 (pt) 2011-09-22 2011-09-22 método para monitorização, diagnóstico e/ou prognóstico de lesão renal aguda no estágio inicial

Country Status (14)

Country Link
US (1) US20140329334A1 (pt)
EP (1) EP2758774A4 (pt)
JP (1) JP5893742B2 (pt)
KR (1) KR20140076571A (pt)
CN (1) CN103917869A (pt)
AU (1) AU2011377379A1 (pt)
BR (1) BR112014006741A2 (pt)
CA (1) CA2849577A1 (pt)
HK (1) HK1199754A1 (pt)
IL (1) IL231626A0 (pt)
IN (1) IN2014DN03194A (pt)
MX (1) MX2014003422A (pt)
RU (1) RU2014115999A (pt)
WO (1) WO2013041913A1 (pt)

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FR3014198B1 (fr) * 2013-12-03 2017-03-03 Biomerieux Sa Procede d'isolement d'exosomes
ES2825708T3 (es) * 2014-07-17 2021-05-17 Univ Pennsylvania Métodos para usar exosomas para monitorizar el estado de órgano trasplantado
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
WO2016077484A1 (en) * 2014-11-11 2016-05-19 Astute Medical, Inc. Methods and compositions for diagnosis and prognosis of renal injury and renal failure
US10370719B2 (en) 2014-11-12 2019-08-06 Hitachi Chemical Co., Ltd. Method and device for diagnosing organ injury
WO2016140388A1 (ko) * 2015-03-05 2016-09-09 경북대학교 산학협력단 엑소좀 또는 이의 단백질을 포함하는 장기 독성 진단용 바이오마커 조성물 및 이를 이용한 진단 방법
US20160320390A1 (en) * 2015-05-01 2016-11-03 Morehouse School Of Medicine Compositions and methods for capturing exosomes
WO2017027858A1 (en) * 2015-08-12 2017-02-16 The Trustees Of Columbia University In The City Of New York Methods of treating volume depletion and kidney injury
JP6624704B2 (ja) 2015-08-31 2019-12-25 日立化成株式会社 尿路上皮疾患の評価のための分子法
JP6531987B2 (ja) * 2015-10-01 2019-06-19 国立大学法人名古屋大学 腎疾患診断のためのエクソソーム回収法
CN105441578B (zh) * 2016-01-19 2018-08-31 中南大学湘雅二医院 尿液外泌体microRNA分子标志物的应用及制备的试剂盒
EP3427060A4 (en) 2016-03-07 2019-12-18 CFGenome, LLC NON-INVASIVE MOLECULAR WITNESSES
CN106053811A (zh) * 2016-06-05 2016-10-26 浙江大学 一种尿液外泌体elisa的检测方法及其应用
RU2647327C2 (ru) * 2016-07-05 2018-03-15 Государственное Бюджетное Образовательное Учреждение Высшего Профессионального Образования "Кубанский государственный медицинский университет" Министерства здравоохранения Российской Федерации (ГБОУ ВПО КубГМУ Минздрава России) Способ ранней диагностики хронической болезни почек у пациентов с хронической обструктивной болезнью легких
JP2019168223A (ja) * 2016-08-12 2019-10-03 公立大学法人和歌山県立医科大学 エクソソームの膜に存在するタンパク質の検出方法
CN109425742A (zh) * 2017-08-23 2019-03-05 迪亚莱博（张家港）生物科技有限公司 一种化学发光法快速检测水通道蛋白1的试剂盒
WO2019045049A1 (ja) * 2017-09-01 2019-03-07 学校法人帝京大学 腎尿細管細胞特異的発現ベクター
RU2702023C1 (ru) * 2018-08-17 2019-10-04 Федеральное государственное бюджетное учреждение "Ростовский научно-исследовательский онкологический институт" Министерства здравоохранения Российской Федерации Способ диагностики острого повреждения почек после органосохраняющего хирургического лечения локализованного рака почки
ES2968898T3 (es) * 2019-06-06 2024-05-14 Fundacion Instituto De Investig Sanitaria Fundacion Jimenez Diaz Método in vitro para detectar enfermedad renal
EP3904883A1 (en) * 2020-04-07 2021-11-03 Sciomics GmbH Prediction and early diagnosis of acute kidney injury
RU2765678C1 (ru) * 2020-12-21 2022-02-01 федеральное государственное бюджетное образовательное учреждение высшего образования "Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. Павлова" Министерства здравоохранения Российской Федерации Способ прогнозирования риска развития острого повреждения почек у больных с острым коронарным синдромом
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Also Published As

Publication number Publication date
EP2758774A1 (en) 2014-07-30
IN2014DN03194A (pt) 2015-05-22
RU2014115999A (ru) 2015-10-27
HK1199754A1 (en) 2015-07-17
CA2849577A1 (en) 2013-03-28
JP5893742B2 (ja) 2016-03-23
KR20140076571A (ko) 2014-06-20
JP2014528076A (ja) 2014-10-23
CN103917869A (zh) 2014-07-09
US20140329334A1 (en) 2014-11-06
EP2758774A4 (en) 2015-04-29
IL231626A0 (en) 2014-05-28
AU2011377379A1 (en) 2014-04-10
WO2013041913A1 (en) 2013-03-28
MX2014003422A (es) 2014-10-13

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