BR112014000352A2 - método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada - Google Patents

método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada

Info

Publication number
BR112014000352A2
BR112014000352A2 BR112014000352A BR112014000352A BR112014000352A2 BR 112014000352 A2 BR112014000352 A2 BR 112014000352A2 BR 112014000352 A BR112014000352 A BR 112014000352A BR 112014000352 A BR112014000352 A BR 112014000352A BR 112014000352 A2 BR112014000352 A2 BR 112014000352A2
Authority
BR
Brazil
Prior art keywords
purified
tnfr
purifying
protein
proteins containing
Prior art date
Application number
BR112014000352A
Other languages
English (en)
Portuguese (pt)
Inventor
Lee Christine
Cynthia Ikechukwu Ijeoma
Nti-Gyabaah Joseph
Petroff Matthew
Original Assignee
Merck Sharp & Dohe Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp & Dohe Corp filed Critical Merck Sharp & Dohe Corp
Publication of BR112014000352A2 publication Critical patent/BR112014000352A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
BR112014000352A 2011-07-08 2012-07-03 método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada BR112014000352A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161505710P 2011-07-08 2011-07-08
PCT/US2012/045339 WO2013009526A1 (en) 2011-07-08 2012-07-03 Method for purifying fc-fusion protein

Publications (1)

Publication Number Publication Date
BR112014000352A2 true BR112014000352A2 (pt) 2017-02-14

Family

ID=47506409

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014000352A BR112014000352A2 (pt) 2011-07-08 2012-07-03 método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada

Country Status (10)

Country Link
US (1) US9556258B2 (cg-RX-API-DMAC10.html)
EP (1) EP2729482B1 (cg-RX-API-DMAC10.html)
JP (1) JP6280499B2 (cg-RX-API-DMAC10.html)
KR (1) KR20140043934A (cg-RX-API-DMAC10.html)
CN (1) CN103814044A (cg-RX-API-DMAC10.html)
AU (2) AU2012282960A1 (cg-RX-API-DMAC10.html)
BR (1) BR112014000352A2 (cg-RX-API-DMAC10.html)
CA (1) CA2840951A1 (cg-RX-API-DMAC10.html)
DK (1) DK2729482T3 (cg-RX-API-DMAC10.html)
WO (1) WO2013009526A1 (cg-RX-API-DMAC10.html)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2012326170B2 (en) 2011-10-18 2016-12-22 Coherus Biosciences, Inc. Etanercept formulations stabilized with metal ions
KR102250937B1 (ko) * 2012-09-11 2021-05-12 코히러스 바이오사이언시즈, 인코포레이티드 고순도 및 탁월한 수율의 정확하게 폴딩된 에타너셉트
PL3395423T3 (pl) * 2013-03-14 2024-03-18 Amgen Inc. Usuwanie wypłukanych ligandów do oczyszczania na zasadzie powinowactwa
SG10201802023RA (en) * 2013-03-26 2018-05-30 Coherus Biosciences Inc Protein production method
NZ722391A (en) * 2014-02-04 2022-12-23 Biogen Ma Inc Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
KR102707370B1 (ko) * 2014-05-13 2024-09-13 암겐 인코포레이티드 필터 및 여과 공정과 함께 사용하기 위한 공정 제어 시스템 및 방법
WO2015189832A1 (en) 2014-06-13 2015-12-17 Lupin Limited Process for the purification of tnfr:fc fusion protein
FR3025515B1 (fr) * 2014-09-05 2016-09-09 Lab Francais Du Fractionnement Procede de purification d'un anticorps monoclonal
WO2016065273A1 (en) * 2014-10-24 2016-04-28 The University Of Chicago Heat-inducible self-assembling protein domains
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
KR102018608B1 (ko) 2014-12-31 2019-09-06 주식회사 엘지화학 목적하는 함량으로 불순물을 포함하는 TNFR-Fc 융합 단백질의 제조방법
CN107921407B (zh) * 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
EP3353288A1 (en) 2015-09-22 2018-08-01 H. Hoffnabb-La Roche Ag Expression of fc-containing proteins
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
CN108463243B (zh) 2015-11-19 2022-06-14 夏尔人类遗传性治疗公司 重组人c1酯酶抑制剂及其用途
EP3455241B1 (en) 2016-05-11 2022-02-23 Cytiva BioProcess R&D AB Method of cleaning and/or sanitizing a separation matrix
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
JP7122974B2 (ja) 2016-05-11 2022-08-22 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 変異免疫グロブリン結合ポリペプチド
WO2017194592A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of storing a separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US11312745B2 (en) * 2016-07-22 2022-04-26 Amgen Inc. Methods of purifying Fc-containing proteins
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
ES3064802T3 (en) * 2016-10-21 2026-04-29 Amgen Inc Pharmaceutical formulations and methods of making the same
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
CN109929027B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
WO2019246153A1 (en) 2018-06-19 2019-12-26 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
EP3941607A1 (en) * 2019-03-21 2022-01-26 Codiak BioSciences, Inc. Process for preparing extracellular vesicles
US20220411465A1 (en) * 2019-11-22 2022-12-29 Morphosys Ag Eluate collection during antibody chromatography
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
US11416468B2 (en) * 2020-07-21 2022-08-16 International Business Machines Corporation Active-active system index management
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
MA71665A (fr) * 2021-02-11 2025-05-30 Denali Therapeutics Inc. Protéines de fusion du récepteur anti-transferrine et leurs procédés d'utilisation
EP4408857A4 (en) * 2021-09-28 2025-11-05 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN
AU2022354256A1 (en) * 2021-09-28 2024-04-11 Kashiv Biosciences, Llc An improved process for purification of fusion protein
CN114891061A (zh) * 2022-04-25 2022-08-12 杭州艾策生物技术有限公司 一种糖基化重组蛋白的纯化方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001509030A (ja) 1997-01-21 2001-07-10 ヒューマン ジノーム サイエンシーズ，インコーポレイテッド Ｆｃレセプターおよびポリペプチド
EP1006183A1 (en) 1998-12-03 2000-06-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Recombinant soluble Fc receptors
JP4363986B2 (ja) * 2001-12-21 2009-11-11 イミュネックス・コーポレーション タンパク質の精製方法
CA2492267C (en) * 2002-07-15 2013-09-10 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
US7427659B2 (en) 2003-10-24 2008-09-23 Amgen Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
CN1946739A (zh) * 2004-04-14 2007-04-11 豪夫迈－罗氏公司 纯化的白细胞介素－15/Fc融合蛋白及其制备
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
KR20090058532A (ko) 2006-08-28 2009-06-09 아레스 트레이딩 에스.에이. Ｆｃ­융합 단백질을 정제하는 방법
WO2008085988A1 (en) 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
WO2010030393A1 (en) 2008-09-15 2010-03-18 Millipore Corporation Methods for quantifying protein leakage from protein based affinity chromatography resins
US20120282654A1 (en) 2009-04-29 2012-11-08 Schering Corporation Antibody purification
US8536316B2 (en) 2009-08-07 2013-09-17 Emd Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production

Also Published As

Publication number Publication date
EP2729482A4 (en) 2015-02-25
JP2014520814A (ja) 2014-08-25
DK2729482T3 (en) 2018-05-07
CA2840951A1 (en) 2013-01-17
AU2017204893A1 (en) 2017-08-03
KR20140043934A (ko) 2014-04-11
EP2729482B1 (en) 2018-03-07
AU2012282960A1 (en) 2014-01-16
JP6280499B2 (ja) 2018-02-14
WO2013009526A1 (en) 2013-01-17
EP2729482A1 (en) 2014-05-14
US9556258B2 (en) 2017-01-31
CN103814044A (zh) 2014-05-21
US20140187751A1 (en) 2014-07-03

Similar Documents

Publication Publication Date Title
IL276534A (en) Methods for the purification of fusion proteins containing serphin
DK2691411T3 (da) Buffersystem til proteinoprensning
EP2729482A4 (en) PROCESS FOR CLEANING FC FUSION PROTEINS
BR112013024521A2 (pt) métodos de purificação de proteínas
DK2526114T3 (da) Kromatografisk fremgangsmåde til oprensning af FC-indeholdende proteiner
DK2598516T3 (da) Fremgangsmåde til proteinoprensning
WO2013138793A3 (en) Affinity reagants for protein purification
IL225650A0 (en) Protein purification processes
PT2768857T (pt) Métodos para purificar anticorpos
PL3299380T3 (pl) Sposoby oczyszczania polipeptydów
DK2598514T3 (da) Proteinoprensning
DK3578659T3 (da) Kunstige nukelinsyremolekyler til forbedret protein- eller peptidekspression
EP2521568A4 (en) PLASMA KALLIKER BINDING PROTEINS
BR112014011900A2 (pt) um método de purificação de anticorpo
EP2663857A4 (en) Protein Detection Method
PL3415531T3 (pl) Białka wiążące antygen
SMT202400377T1 (it) Proteine contenenti fc di anticorpi eterodimerici e metodi per la loro produzione
EP2953965A4 (en) PROTEIN PURIFICATION PROCESS
BR112014001120A2 (pt) métodos de separação de polipeptídeos
EP2727930A4 (en) PROCESS FOR CLEANING PROTEINS
PL2776460T3 (pl) Białko fuzyjne zawierające interleukinę 4 i interleukinę 10
EP2698386A4 (en) FUSION PROTEIN
SG10201606218WA (en) Purified Proteins
IL232834A0 (en) Anti-cancer fusion protein
IL233999A0 (en) Ligands for binding antibodies and protein conjugates containing antibodies and their use in chromatography

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NAO 10196/2001, QUE MODIFICOU A LEI NAO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUAANCIA PRA VIA DA ANVISA. CONSIDERANDO A APROVAA AO DOS TERMOS DO PARECER NAO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NAO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDAANCIAS CABA-VEIS.

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 8A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2594 DE 24-09-2020 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.