KR20140043934A - Ｆｃ-융합 단백질의 정제 방법 - Google Patents

Ｆｃ-융합 단백질의 정제 방법 Download PDF

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Publication number
KR20140043934A
KR20140043934A KR1020147002981A KR20147002981A KR20140043934A KR 20140043934 A KR20140043934 A KR 20140043934A KR 1020147002981 A KR1020147002981 A KR 1020147002981A KR 20147002981 A KR20147002981 A KR 20147002981A KR 20140043934 A KR20140043934 A KR 20140043934A
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South Korea
Prior art keywords
protein
chromatography
fusion protein
product
elution
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KR1020147002981A
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English (en)
Korean (ko)
Inventor
이제오마 신시아 이케추쿠
조셉 티-갸바
메튜 페트로프
크리스틴 리
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머크 샤프 앤드 돔 코포레이션
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Publication of KR20140043934A publication Critical patent/KR20140043934A/ko
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
KR1020147002981A 2011-07-08 2012-07-03 Ｆｃ-융합 단백질의 정제 방법 Withdrawn KR20140043934A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161505710P 2011-07-08 2011-07-08
US61/505,710 2011-07-08
PCT/US2012/045339 WO2013009526A1 (en) 2011-07-08 2012-07-03 Method for purifying fc-fusion protein

Publications (1)

Publication Number Publication Date
KR20140043934A true KR20140043934A (ko) 2014-04-11

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ID=47506409

Family Applications (1)

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KR1020147002981A Withdrawn KR20140043934A (ko) 2011-07-08 2012-07-03 Ｆｃ-융합 단백질의 정제 방법

Country Status (10)

Country Link
US (1) US9556258B2 (cg-RX-API-DMAC10.html)
EP (1) EP2729482B1 (cg-RX-API-DMAC10.html)
JP (1) JP6280499B2 (cg-RX-API-DMAC10.html)
KR (1) KR20140043934A (cg-RX-API-DMAC10.html)
CN (1) CN103814044A (cg-RX-API-DMAC10.html)
AU (2) AU2012282960A1 (cg-RX-API-DMAC10.html)
BR (1) BR112014000352A2 (cg-RX-API-DMAC10.html)
CA (1) CA2840951A1 (cg-RX-API-DMAC10.html)
DK (1) DK2729482T3 (cg-RX-API-DMAC10.html)
WO (1) WO2013009526A1 (cg-RX-API-DMAC10.html)

Cited By (2)

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Publication number Priority date Publication date Assignee Title
KR20150056601A (ko) * 2012-09-11 2015-05-26 코히러스 바이오사이언시즈, 인코포레이티드 고순도 및 탁월한 수율의 정확하게 폴딩된 에타너셉트
WO2016108569A1 (ko) * 2014-12-31 2016-07-07 주식회사 엘지생명과학 목적하는 함량으로 불순물을 포함하는 tnfr-fc 융합 단백질의 제조방법

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PL3395423T3 (pl) * 2013-03-14 2024-03-18 Amgen Inc. Usuwanie wypłukanych ligandów do oczyszczania na zasadzie powinowactwa
SG10201802023RA (en) * 2013-03-26 2018-05-30 Coherus Biosciences Inc Protein production method
NZ722391A (en) * 2014-02-04 2022-12-23 Biogen Ma Inc Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
KR102707370B1 (ko) * 2014-05-13 2024-09-13 암겐 인코포레이티드 필터 및 여과 공정과 함께 사용하기 위한 공정 제어 시스템 및 방법
WO2015189832A1 (en) 2014-06-13 2015-12-17 Lupin Limited Process for the purification of tnfr:fc fusion protein
FR3025515B1 (fr) * 2014-09-05 2016-09-09 Lab Francais Du Fractionnement Procede de purification d'un anticorps monoclonal
WO2016065273A1 (en) * 2014-10-24 2016-04-28 The University Of Chicago Heat-inducible self-assembling protein domains
US20160130324A1 (en) 2014-10-31 2016-05-12 Shire Human Genetic Therapies, Inc. C1 Inhibitor Fusion Proteins and Uses Thereof
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
CN107921407B (zh) * 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
EP3353288A1 (en) 2015-09-22 2018-08-01 H. Hoffnabb-La Roche Ag Expression of fc-containing proteins
US20170101435A1 (en) * 2015-10-13 2017-04-13 Therapeutic Proteins International, LLC Harvesting and perfusion apparatus
CN108463243B (zh) 2015-11-19 2022-06-14 夏尔人类遗传性治疗公司 重组人c1酯酶抑制剂及其用途
EP3455241B1 (en) 2016-05-11 2022-02-23 Cytiva BioProcess R&D AB Method of cleaning and/or sanitizing a separation matrix
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
JP7122974B2 (ja) 2016-05-11 2022-08-22 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 変異免疫グロブリン結合ポリペプチド
WO2017194592A1 (en) 2016-05-11 2017-11-16 Ge Healthcare Bioprocess R&D Ab Method of storing a separation matrix
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
US11312745B2 (en) * 2016-07-22 2022-04-26 Amgen Inc. Methods of purifying Fc-containing proteins
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
ES3064802T3 (en) * 2016-10-21 2026-04-29 Amgen Inc Pharmaceutical formulations and methods of making the same
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
CN109929027B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 采用线性洗脱步骤的重组融合蛋白纯化方法
WO2019246153A1 (en) 2018-06-19 2019-12-26 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
EP3941607A1 (en) * 2019-03-21 2022-01-26 Codiak BioSciences, Inc. Process for preparing extracellular vesicles
US20220411465A1 (en) * 2019-11-22 2022-12-29 Morphosys Ag Eluate collection during antibody chromatography
AU2021262609A1 (en) 2020-05-01 2022-12-22 Kashiv Biosciences, Llc An improved process of purification of protein
US11416468B2 (en) * 2020-07-21 2022-08-16 International Business Machines Corporation Active-active system index management
CN112574321B (zh) * 2020-12-30 2023-10-20 上海赛金生物医药有限公司 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法
MA71665A (fr) * 2021-02-11 2025-05-30 Denali Therapeutics Inc. Protéines de fusion du récepteur anti-transferrine et leurs procédés d'utilisation
EP4408857A4 (en) * 2021-09-28 2025-11-05 Kashiv Biosciences Llc IMPROVED PROCESS FOR PURIFICATION OF FUSION PROTEIN
AU2022354256A1 (en) * 2021-09-28 2024-04-11 Kashiv Biosciences, Llc An improved process for purification of fusion protein
CN114891061A (zh) * 2022-04-25 2022-08-12 杭州艾策生物技术有限公司 一种糖基化重组蛋白的纯化方法

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JP2001509030A (ja) 1997-01-21 2001-07-10 ヒューマン ジノーム サイエンシーズ，インコーポレイテッド Ｆｃレセプターおよびポリペプチド
EP1006183A1 (en) 1998-12-03 2000-06-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Recombinant soluble Fc receptors
JP4363986B2 (ja) * 2001-12-21 2009-11-11 イミュネックス・コーポレーション タンパク質の精製方法
CA2492267C (en) * 2002-07-15 2013-09-10 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
US7427659B2 (en) 2003-10-24 2008-09-23 Amgen Inc. Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction
CN1946739A (zh) * 2004-04-14 2007-04-11 豪夫迈－罗氏公司 纯化的白细胞介素－15/Fc融合蛋白及其制备
JP2010501622A (ja) * 2006-08-28 2010-01-21 アレス トレーディング ソシエテ アノニム Ｆｃ−融合タンパク質の精製法
KR20090058532A (ko) 2006-08-28 2009-06-09 아레스 트레이딩 에스.에이. Ｆｃ­융합 단백질을 정제하는 방법
WO2008085988A1 (en) 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
WO2010030393A1 (en) 2008-09-15 2010-03-18 Millipore Corporation Methods for quantifying protein leakage from protein based affinity chromatography resins
US20120282654A1 (en) 2009-04-29 2012-11-08 Schering Corporation Antibody purification
US8536316B2 (en) 2009-08-07 2013-09-17 Emd Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
US9540426B2 (en) 2009-10-06 2017-01-10 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150056601A (ko) * 2012-09-11 2015-05-26 코히러스 바이오사이언시즈, 인코포레이티드 고순도 및 탁월한 수율의 정확하게 폴딩된 에타너셉트
WO2016108569A1 (ko) * 2014-12-31 2016-07-07 주식회사 엘지생명과학 목적하는 함량으로 불순물을 포함하는 tnfr-fc 융합 단백질의 제조방법
US10988527B2 (en) 2014-12-31 2021-04-27 Lg Chem, Ltd. Method for preparing TNFR-Fc fusion protein containing target content of impurities

Also Published As

Publication number Publication date
EP2729482A4 (en) 2015-02-25
JP2014520814A (ja) 2014-08-25
DK2729482T3 (en) 2018-05-07
CA2840951A1 (en) 2013-01-17
AU2017204893A1 (en) 2017-08-03
EP2729482B1 (en) 2018-03-07
AU2012282960A1 (en) 2014-01-16
BR112014000352A2 (pt) 2017-02-14
JP6280499B2 (ja) 2018-02-14
WO2013009526A1 (en) 2013-01-17
EP2729482A1 (en) 2014-05-14
US9556258B2 (en) 2017-01-31
CN103814044A (zh) 2014-05-21
US20140187751A1 (en) 2014-07-03

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Patent event date: 20140205

Patent event code: PA01051R01D

Comment text: International Patent Application

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PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20170619

Comment text: Request for Examination of Application

PC1202 Submission of document of withdrawal before decision of registration

Comment text: [Withdrawal of Procedure relating to Patent, etc.] Withdrawal (Abandonment)

Patent event code: PC12021R01D

Patent event date: 20180615

WITB Written withdrawal of application