BR112013032338A8 - Método para melhorar o rendimento de produção para proteína l1 do papilomavírus humano - Google Patents
Método para melhorar o rendimento de produção para proteína l1 do papilomavírus humanoInfo
- Publication number
- BR112013032338A8 BR112013032338A8 BR112013032338A BR112013032338A BR112013032338A8 BR 112013032338 A8 BR112013032338 A8 BR 112013032338A8 BR 112013032338 A BR112013032338 A BR 112013032338A BR 112013032338 A BR112013032338 A BR 112013032338A BR 112013032338 A8 BR112013032338 A8 BR 112013032338A8
- Authority
- BR
- Brazil
- Prior art keywords
- protein
- production yield
- hpv
- human papillomavirus
- improve production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/01—DNA viruses
- C07K14/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/20011—Papillomaviridae
- C12N2710/20051—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
Método para melhorar o rendimento de produção da proteína L1 do papilomavírus humano. A presente invenção é um método para melhorar o rendimento da produção de proteína L 1 de HPV, incluindo a fase de cultura de células que expressam a proteína L1 de HPV, em um meio contendo alta concentração da fonte de carbono. De acordo com o método de cultura utilizando um meio contendo fontes de carbono altamente concentradas da presente invenção, o rendimento de produção da proteína L1 de HPV pode ser não só extremamente aumentado, mas também a 10 imunogenicidade da proteína L1 de HPV produzida é significativamente aumentada.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20110057748 | 2011-06-15 | ||
| KR10-2011-0057748 | 2011-06-15 | ||
| PCT/KR2012/004667 WO2012173390A2 (ko) | 2011-06-15 | 2012-06-13 | 인유두종바이러스 l1 단백질의 생산 수율을 향상시키는 방법 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR112013032338A2 BR112013032338A2 (pt) | 2016-12-20 |
| BR112013032338A8 true BR112013032338A8 (pt) | 2018-02-06 |
| BR112013032338B1 BR112013032338B1 (pt) | 2021-04-20 |
Family
ID=47357597
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112013032338-8A BR112013032338B1 (pt) | 2011-06-15 | 2012-06-13 | método para melhorar o rendimento de produção para proteína l1 do papilomavírus humano |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US8871466B2 (pt) |
| EP (1) | EP2722389B1 (pt) |
| JP (1) | JP6001064B2 (pt) |
| KR (1) | KR101458270B1 (pt) |
| CN (1) | CN103717741B (pt) |
| AU (1) | AU2012270418B2 (pt) |
| BR (1) | BR112013032338B1 (pt) |
| CA (1) | CA2839427C (pt) |
| CL (1) | CL2013003545A1 (pt) |
| MX (1) | MX348241B (pt) |
| WO (1) | WO2012173390A2 (pt) |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL117459A (en) * | 1995-03-22 | 2005-11-20 | Merck & Co Inc | Dna encoding human papillomavirus type 18 |
| IL117591A0 (en) * | 1995-03-30 | 1996-07-23 | Merck & Co Inc | Synthetic DNA encoding human papillomavirus type 11 L1 protein |
| MY140664A (en) * | 2003-09-29 | 2010-01-15 | Merck Sharp & Dohme | Optimized expression of hpv 45 l1 in yeast |
| MY139500A (en) * | 2003-11-12 | 2009-10-30 | Merck Sharp & Dohme | Optimized expression of hpv 58 l1 in yeast |
| KR100545563B1 (ko) * | 2003-12-23 | 2006-01-24 | 충남대학교산학협력단 | 재조합 단백질 생산 능력이 향상된 사카로마이세스세레비지애 변이주 |
| KR100595864B1 (ko) | 2004-01-27 | 2006-06-30 | 재단법인 목암생명공학연구소 | 형질전환 사카로마이세스 세레비지애 균주 및 이를 이용한ｌｋ8 단백질의 생산방법 |
| EP2041280B1 (en) * | 2006-06-20 | 2016-08-10 | Mogam Biotechnology Research Institute | Methods for enhancing a secretion efficiency of recombinant foreign protein in yeast expression system |
| KR100959145B1 (ko) * | 2008-03-21 | 2010-05-25 | 중앙대학교 산학협력단 | 인유두종바이러스 바이러스 유사 입자의 생산 및 정제 방법 |
| EP2444103B1 (en) * | 2009-06-19 | 2017-12-20 | Eyegene Inc. | Vaccine for cervical cancer |
-
2012
- 2012-06-13 AU AU2012270418A patent/AU2012270418B2/en active Active
- 2012-06-13 MX MX2013014899A patent/MX348241B/es active IP Right Grant
- 2012-06-13 WO PCT/KR2012/004667 patent/WO2012173390A2/ko active Application Filing
- 2012-06-13 KR KR1020120063107A patent/KR101458270B1/ko active IP Right Grant
- 2012-06-13 EP EP12801099.8A patent/EP2722389B1/en active Active
- 2012-06-13 JP JP2014515747A patent/JP6001064B2/ja active Active
- 2012-06-13 CA CA2839427A patent/CA2839427C/en active Active
- 2012-06-13 BR BR112013032338-8A patent/BR112013032338B1/pt active IP Right Grant
- 2012-06-13 US US14/126,091 patent/US8871466B2/en active Active
- 2012-06-13 CN CN201280029590.XA patent/CN103717741B/zh active Active
-
2013
- 2013-12-10 CL CL2013003545A patent/CL2013003545A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| BR112013032338A2 (pt) | 2016-12-20 |
| EP2722389A2 (en) | 2014-04-23 |
| EP2722389A4 (en) | 2015-01-21 |
| MX348241B (es) | 2017-06-01 |
| AU2012270418B2 (en) | 2015-08-13 |
| WO2012173390A3 (ko) | 2013-03-14 |
| US8871466B2 (en) | 2014-10-28 |
| CN103717741B (zh) | 2016-04-13 |
| MX2013014899A (es) | 2014-02-17 |
| WO2012173390A2 (ko) | 2012-12-20 |
| CN103717741A (zh) | 2014-04-09 |
| CL2013003545A1 (es) | 2014-07-11 |
| CA2839427A1 (en) | 2012-12-20 |
| EP2722389B1 (en) | 2018-03-07 |
| BR112013032338B1 (pt) | 2021-04-20 |
| JP2014519339A (ja) | 2014-08-14 |
| JP6001064B2 (ja) | 2016-10-05 |
| CA2839427C (en) | 2016-09-27 |
| KR101458270B1 (ko) | 2014-11-10 |
| US20140186888A1 (en) | 2014-07-03 |
| KR20120138683A (ko) | 2012-12-26 |
| AU2012270418A1 (en) | 2014-01-16 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25A | Requested transfer of rights approved |
Owner name: HONG JIN KIM (KR) |
|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B25A | Requested transfer of rights approved |
Owner name: POSVAX CO., LTD (KR) |
|
| B06T | Formal requirements before examination [chapter 6.20 patent gazette] | ||
| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] |
Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS. |
|
| B07E | Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/06/2012, OBSERVADAS AS CONDICOES LEGAIS. |