MX348241B - Metodo para mejorar el rendimiento de produccion de la proteina l1 del virus del papiloma humano. - Google Patents

Metodo para mejorar el rendimiento de produccion de la proteina l1 del virus del papiloma humano.

Info

Publication number
MX348241B
MX348241B MX2013014899A MX2013014899A MX348241B MX 348241 B MX348241 B MX 348241B MX 2013014899 A MX2013014899 A MX 2013014899A MX 2013014899 A MX2013014899 A MX 2013014899A MX 348241 B MX348241 B MX 348241B
Authority
MX
Mexico
Prior art keywords
protein
production yield
enhancing
human papillomavirus
hpv
Prior art date
Application number
MX2013014899A
Other languages
English (en)
Other versions
MX2013014899A (es
Inventor
Hyoung Jin Kim
Hong Jin Kim
Original Assignee
Hong Jin Kim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hong Jin Kim filed Critical Hong Jin Kim
Publication of MX2013014899A publication Critical patent/MX2013014899A/es
Publication of MX348241B publication Critical patent/MX348241B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/01—DNA viruses
    • C07K14/025—Papovaviridae, e.g. papillomavirus, polyomavirus, SV40, BK virus, JC virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/20011—Papillomaviridae
    • C12N2710/20051—Methods of production or purification of viral material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para mejorar el rendimiento de producción de la proteína L1 del HPV que incluye la fase de cultivar la célula que expresa la proteína L1 del HPV en un medio que contiene concentración alta de fuente de carbono; de conformidad con el método de cultivo que usa un medio que contiene fuente de carbono altamente concentrada de esta invención, el rendimiento de producción de la proteína L1 del HPV puede no solo incrementarse marcadamente, sino que también la inmunogenicidad de la proteína L1 del HPV producida se Incrementa significativamente.
MX2013014899A 2011-06-15 2012-06-13 Metodo para mejorar el rendimiento de produccion de la proteina l1 del virus del papiloma humano. MX348241B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20110057748 2011-06-15
PCT/KR2012/004667 WO2012173390A2 (ko) 2011-06-15 2012-06-13 인유두종바이러스 l1 단백질의 생산 수율을 향상시키는 방법

Publications (2)

Publication Number Publication Date
MX2013014899A MX2013014899A (es) 2014-02-17
MX348241B true MX348241B (es) 2017-06-01

Family

ID=47357597

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013014899A MX348241B (es) 2011-06-15 2012-06-13 Metodo para mejorar el rendimiento de produccion de la proteina l1 del virus del papiloma humano.

Country Status (11)

Country Link
US (1) US8871466B2 (es)
EP (1) EP2722389B1 (es)
JP (1) JP6001064B2 (es)
KR (1) KR101458270B1 (es)
CN (1) CN103717741B (es)
AU (1) AU2012270418B2 (es)
BR (1) BR112013032338B1 (es)
CA (1) CA2839427C (es)
CL (1) CL2013003545A1 (es)
MX (1) MX348241B (es)
WO (1) WO2012173390A2 (es)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL117459A (en) * 1995-03-22 2005-11-20 Merck & Co Inc Dna encoding human papillomavirus type 18
IL117591A0 (en) * 1995-03-30 1996-07-23 Merck & Co Inc Synthetic DNA encoding human papillomavirus type 11 L1 protein
MY140664A (en) * 2003-09-29 2010-01-15 Merck Sharp & Dohme Optimized expression of hpv 45 l1 in yeast
MY139500A (en) * 2003-11-12 2009-10-30 Merck Sharp & Dohme Optimized expression of hpv 58 l1 in yeast
KR100545563B1 (ko) * 2003-12-23 2006-01-24 충남대학교산학협력단 재조합 단백질 생산 능력이 향상된 사카로마이세스세레비지애 변이주
KR100595864B1 (ko) * 2004-01-27 2006-06-30 재단법인 목암생명공학연구소 형질전환 사카로마이세스 세레비지애 균주 및 이를 이용한ｌｋ8 단백질의 생산방법
US8298787B2 (en) * 2006-06-20 2012-10-30 Mogam Biotechnology Research Institute Methods for enhancing a secretion efficiency of recombinant foreign protein in yeast expression system
KR100959145B1 (ko) * 2008-03-21 2010-05-25 중앙대학교 산학협력단 인유두종바이러스 바이러스 유사 입자의 생산 및 정제 방법
MX2011013566A (es) * 2009-06-19 2012-06-28 Eyegene Inc Vacunas para cancer cervical.

Also Published As

Publication number Publication date
AU2012270418A1 (en) 2014-01-16
US20140186888A1 (en) 2014-07-03
WO2012173390A2 (ko) 2012-12-20
CN103717741A (zh) 2014-04-09
CA2839427A1 (en) 2012-12-20
EP2722389B1 (en) 2018-03-07
CL2013003545A1 (es) 2014-07-11
AU2012270418B2 (en) 2015-08-13
KR101458270B1 (ko) 2014-11-10
BR112013032338A2 (pt) 2016-12-20
KR20120138683A (ko) 2012-12-26
EP2722389A4 (en) 2015-01-21
EP2722389A2 (en) 2014-04-23
BR112013032338B1 (pt) 2021-04-20
WO2012173390A3 (ko) 2013-03-14
CN103717741B (zh) 2016-04-13
CA2839427C (en) 2016-09-27
MX2013014899A (es) 2014-02-17
JP2014519339A (ja) 2014-08-14
US8871466B2 (en) 2014-10-28
JP6001064B2 (ja) 2016-10-05
BR112013032338A8 (pt) 2018-02-06

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