BR112013014457A2 - método com base em mgmt para obter alta produção de expressão de proteína recombinante - Google Patents
método com base em mgmt para obter alta produção de expressão de proteína recombinanteInfo
- Publication number
- BR112013014457A2 BR112013014457A2 BR112013014457A BR112013014457A BR112013014457A2 BR 112013014457 A2 BR112013014457 A2 BR 112013014457A2 BR 112013014457 A BR112013014457 A BR 112013014457A BR 112013014457 A BR112013014457 A BR 112013014457A BR 112013014457 A2 BR112013014457 A2 BR 112013014457A2
- Authority
- BR
- Brazil
- Prior art keywords
- mgmt
- protein expression
- recombinant protein
- obtain high
- high production
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0601—Invertebrate cells or tissues, e.g. insect cells; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
- C12N9/1007—Methyltransferases (general) (2.1.1.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y201/00—Transferases transferring one-carbon groups (2.1)
- C12Y201/01—Methyltransferases (2.1.1)
- C12Y201/01063—Methylated-DNA-[protein]-cysteine S-methyltransferase (2.1.1.63), i.e. O6-methylguanine-DNA methyltransferase
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/055—Fusion polypeptide containing a localisation/targetting motif containing a signal for localisation to secretory granules (for exocytosis)
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
- C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/103—Plasmid DNA for invertebrates
- C12N2800/105—Plasmid DNA for invertebrates for insects
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Oncology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10306389 | 2010-12-09 | ||
US201161505694P | 2011-07-08 | 2011-07-08 | |
PCT/EP2011/072387 WO2012076715A1 (en) | 2010-12-09 | 2011-12-09 | Mgmt-based method for obtaining high yield of recombinant protein expression |
Publications (2)
Publication Number | Publication Date |
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BR112013014457A2 true BR112013014457A2 (pt) | 2016-09-27 |
BR112013014457A8 BR112013014457A8 (pt) | 2017-12-19 |
Family
ID=44279107
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112013014457A BR112013014457A8 (pt) | 2010-12-09 | 2011-12-09 | usos da enzima 6-metilguanina-dna-metiltrasferase (mgmt, ec 2.1.1.63) para aumentar a produção de proteína heteróloga, bem como vetor de expressão, células recombinantes e polipeptídeo de fusão compreendendo sinal de secreção peptídica e proteína snap |
Country Status (8)
Country | Link |
---|---|
US (3) | US9109219B2 (pt) |
EP (2) | EP2649178B8 (pt) |
JP (2) | JP5940554B2 (pt) |
CN (3) | CN105821078A (pt) |
BR (1) | BR112013014457A8 (pt) |
CA (1) | CA2819552C (pt) |
ES (1) | ES2627117T3 (pt) |
WO (1) | WO2012076715A1 (pt) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105821078A (zh) | 2010-12-09 | 2016-08-03 | 巴斯德研究所 | 用于获得高产量重组蛋白表达的基于mgmt的方法 |
EP2567968A1 (en) | 2011-09-09 | 2013-03-13 | Institut Pasteur | Polypeptides targeting glycosylated Muc2 proteins, methods of synthesis, their nucleic acids and uses therof |
CN104114697A (zh) | 2011-12-09 | 2014-10-22 | 巴斯德研究所 | 多重免疫筛选分析 |
JP6412131B2 (ja) * | 2013-11-29 | 2018-10-24 | ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア | MERS‐CoVワクチン |
EP3419660A4 (en) * | 2016-02-25 | 2019-11-06 | The Trustees Of The University Of Pennsylvania | NEW VACCINES AGAINST ZIKA VIRUS |
CN105861753A (zh) * | 2016-05-25 | 2016-08-17 | 深圳市第三人民医院 | 检测寨卡病毒的巢式rt-pcr方法、引物及试剂盒 |
FR3052779B1 (fr) * | 2016-06-17 | 2018-06-22 | Universite De Tours | Nouvelles proteines de fusion et leur application pour la preparation de vaccins |
CN106226529B (zh) * | 2016-07-14 | 2018-03-06 | 四川农业大学 | 脑多头蚴病的标志物ldh以及用于诊断脑多头蚴病的试剂盒 |
BR112019005378A2 (pt) * | 2016-09-19 | 2019-08-27 | Inovio Pharmaceuticals Inc | molécula de ácido nucleico, composição, e, métodos para prevenir ou tratar uma doença e para induzir uma resposta imune |
JP7424968B2 (ja) | 2017-09-13 | 2024-01-30 | バイオエヌテック エスエー | 細胞におけるrna発現を増強する方法 |
CN107828738A (zh) * | 2017-11-28 | 2018-03-23 | 新乡医学院 | 一种dna甲基转移酶缺陷型cho细胞系及其制备方法及应用 |
CN110531077B (zh) * | 2018-05-25 | 2023-07-07 | 荣昌生物制药(烟台)股份有限公司 | 间皮素免疫组化检测试剂盒 |
WO2020034097A1 (en) * | 2018-08-14 | 2020-02-20 | Wuxi Biologics (Shanghai) Co., Ltd. | Transcriptional regulatory element and its use in enhancing the expression of exogenous protein |
PT115073A (pt) | 2018-10-09 | 2020-05-29 | Inst De Biologia Molecular E Celular Ibmc | Ácido nucleico para ativar a expressão e a produção de proteínas |
KR102365464B1 (ko) * | 2019-12-24 | 2022-02-22 | 강원대학교산학협력단 | 지카바이러스 재조합 서브유닛 백신의 개발 및 이의 제조방법 |
US11740240B2 (en) * | 2020-07-20 | 2023-08-29 | Bio-Rad Laboratories, Inc. | Immunoassay for SARS-CoV-2 neutralizing antibodies and materials therefor |
CN112359063A (zh) * | 2020-11-04 | 2021-02-12 | 安徽环球基因科技有限公司 | 新冠重组rbd蛋白在昆虫细胞中的生产方法 |
CN114908119A (zh) * | 2021-02-09 | 2022-08-16 | 佛山汉腾生物科技有限公司 | 一种提高重组蛋白表达量的方法 |
CN115976104A (zh) * | 2023-01-03 | 2023-04-18 | 中国食品药品检定研究院 | 一种纯化蛋白的方法 |
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GB2125047B (en) | 1982-08-09 | 1986-02-19 | Ciba Geigy Ag | Yeast hybrid vectors and their use for the production of polypeptides |
EP0118551A1 (en) | 1982-09-15 | 1984-09-19 | Collaborative Research Inc. | Production of interferon in yeast by use of invertase promoter |
US4758512A (en) | 1984-03-06 | 1988-07-19 | President And Fellows Of Harvard College | Hosts and methods for producing recombinant products in high yields |
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US5681713A (en) | 1987-05-08 | 1997-10-28 | Smithkline Beecham Corporation | Expression of heterologous proteins in Drosophila cells |
US5550043A (en) | 1987-05-08 | 1996-08-27 | Smithkline Beecham Corporation | Expression of heterologous proteins in Drosophila cells |
US5879926A (en) | 1989-03-31 | 1999-03-09 | Transgene S.A. | Yeast strains for the production of mature heterologous proteins, especially hirudin |
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WO1998017684A2 (en) * | 1996-10-25 | 1998-04-30 | Advanced Research & Technology Institute | Dna sequences encoding fusions of dna repair proteins and uses thereof |
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EP1155120B1 (fr) | 1999-02-22 | 2006-07-05 | Transgene S.A. | Procede d'obtention d'une preparation virale purifiee |
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EP1266018B1 (en) * | 2000-03-24 | 2008-05-07 | Genencor International, Inc. | Production of secreted proteins by recombinant eukaryotic cells |
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CA2693155A1 (en) | 2007-07-25 | 2009-01-29 | Fraunhofer Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Self coupling recombinant antibody fusion proteins |
FR2934684B1 (fr) | 2008-07-31 | 2012-11-16 | Cis Bio Int | Methode de detection de l'internalisation de proteines membranaires. |
CN105821078A (zh) * | 2010-12-09 | 2016-08-03 | 巴斯德研究所 | 用于获得高产量重组蛋白表达的基于mgmt的方法 |
CN104114697A (zh) * | 2011-12-09 | 2014-10-22 | 巴斯德研究所 | 多重免疫筛选分析 |
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2011
- 2011-12-09 CN CN201610082061.6A patent/CN105821078A/zh active Pending
- 2011-12-09 WO PCT/EP2011/072387 patent/WO2012076715A1/en active Application Filing
- 2011-12-09 CA CA2819552A patent/CA2819552C/en active Active
- 2011-12-09 EP EP11791602.3A patent/EP2649178B8/en active Active
- 2011-12-09 JP JP2013542568A patent/JP5940554B2/ja active Active
- 2011-12-09 US US13/824,476 patent/US9109219B2/en active Active
- 2011-12-09 BR BR112013014457A patent/BR112013014457A8/pt not_active Application Discontinuation
- 2011-12-09 CN CN201710166781.5A patent/CN107090441A/zh active Pending
- 2011-12-09 EP EP16207377.9A patent/EP3202897B1/en active Active
- 2011-12-09 CN CN201180065989.9A patent/CN103476928B/zh active Active
- 2011-12-09 ES ES11791602T patent/ES2627117T3/es active Active
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2015
- 2015-07-28 US US14/811,079 patent/US9546380B2/en active Active
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2016
- 2016-02-29 JP JP2016038333A patent/JP6316856B2/ja active Active
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JP2016154543A (ja) | 2016-09-01 |
EP3202897B1 (en) | 2020-04-15 |
CA2819552C (en) | 2023-09-26 |
ES2627117T3 (es) | 2017-07-26 |
CN103476928A (zh) | 2013-12-25 |
JP2014502163A (ja) | 2014-01-30 |
JP5940554B2 (ja) | 2016-06-29 |
EP2649178B8 (en) | 2017-08-30 |
CA2819552A1 (en) | 2012-06-14 |
US9109219B2 (en) | 2015-08-18 |
BR112013014457A8 (pt) | 2017-12-19 |
EP3202897A1 (en) | 2017-08-09 |
EP2649178A1 (en) | 2013-10-16 |
JP6316856B2 (ja) | 2018-04-25 |
US9546380B2 (en) | 2017-01-17 |
EP2649178B1 (en) | 2017-03-15 |
US20170088843A1 (en) | 2017-03-30 |
CN105821078A (zh) | 2016-08-03 |
CN103476928B (zh) | 2017-03-29 |
US10017769B2 (en) | 2018-07-10 |
WO2012076715A1 (en) | 2012-06-14 |
US20130309747A1 (en) | 2013-11-21 |
US20160017367A1 (en) | 2016-01-21 |
CN107090441A (zh) | 2017-08-25 |
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