BR112013014457A2 - método com base em mgmt para obter alta produção de expressão de proteína recombinante - Google Patents

método com base em mgmt para obter alta produção de expressão de proteína recombinante

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Publication number
BR112013014457A2
BR112013014457A2 BR112013014457A BR112013014457A BR112013014457A2 BR 112013014457 A2 BR112013014457 A2 BR 112013014457A2 BR 112013014457 A BR112013014457 A BR 112013014457A BR 112013014457 A BR112013014457 A BR 112013014457A BR 112013014457 A2 BR112013014457 A2 BR 112013014457A2
Authority
BR
Brazil
Prior art keywords
mgmt
protein expression
recombinant protein
obtain high
high production
Prior art date
Application number
BR112013014457A
Other languages
English (en)
Other versions
BR112013014457A8 (pt
Inventor
Elodie Crublet
Philippe Despres
Sylvie Paulous
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Publication of BR112013014457A2 publication Critical patent/BR112013014457A2/pt
Publication of BR112013014457A8 publication Critical patent/BR112013014457A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0601—Invertebrate cells or tissues, e.g. insect cells; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y201/00—Transferases transferring one-carbon groups (2.1)
    • C12Y201/01—Methyltransferases (2.1.1)
    • C12Y201/01063—Methylated-DNA-[protein]-cysteine S-methyltransferase (2.1.1.63), i.e. O6-methylguanine-DNA methyltransferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/055—Fusion polypeptide containing a localisation/targetting motif containing a signal for localisation to secretory granules (for exocytosis)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/103—Plasmid DNA for invertebrates
    • C12N2800/105—Plasmid DNA for invertebrates for insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Oncology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
BR112013014457A 2010-12-09 2011-12-09 usos da enzima 6-metilguanina-dna-metiltrasferase (mgmt, ec 2.1.1.63) para aumentar a produção de proteína heteróloga, bem como vetor de expressão, células recombinantes e polipeptídeo de fusão compreendendo sinal de secreção peptídica e proteína snap BR112013014457A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP10306389 2010-12-09
US201161505694P 2011-07-08 2011-07-08
PCT/EP2011/072387 WO2012076715A1 (en) 2010-12-09 2011-12-09 Mgmt-based method for obtaining high yield of recombinant protein expression

Publications (2)

Publication Number Publication Date
BR112013014457A2 true BR112013014457A2 (pt) 2016-09-27
BR112013014457A8 BR112013014457A8 (pt) 2017-12-19

Family

ID=44279107

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013014457A BR112013014457A8 (pt) 2010-12-09 2011-12-09 usos da enzima 6-metilguanina-dna-metiltrasferase (mgmt, ec 2.1.1.63) para aumentar a produção de proteína heteróloga, bem como vetor de expressão, células recombinantes e polipeptídeo de fusão compreendendo sinal de secreção peptídica e proteína snap

Country Status (8)

Country Link
US (3) US9109219B2 (pt)
EP (2) EP2649178B8 (pt)
JP (2) JP5940554B2 (pt)
CN (3) CN107090441A (pt)
BR (1) BR112013014457A8 (pt)
CA (1) CA2819552C (pt)
ES (1) ES2627117T3 (pt)
WO (1) WO2012076715A1 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107090441A (zh) * 2010-12-09 2017-08-25 巴斯德研究所 用于获得高产量重组蛋白表达的基于mgmt的方法
EP2567968A1 (en) 2011-09-09 2013-03-13 Institut Pasteur Polypeptides targeting glycosylated Muc2 proteins, methods of synthesis, their nucleic acids and uses therof
JP6215223B2 (ja) * 2011-12-09 2017-10-18 アンスティテュ・パストゥール マルチプレックス免疫スクリーニングアッセイ
AU2014354797C1 (en) * 2013-11-29 2018-02-01 Inovio Pharmaceuticals, Inc. MERS-CoV vaccine
KR20190003471A (ko) * 2016-02-25 2019-01-09 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 지카 바이러스에 대항하는 신규한 백신
CN105861753A (zh) * 2016-05-25 2016-08-17 深圳市第三人民医院 检测寨卡病毒的巢式rt-pcr方法、引物及试剂盒
FR3052779B1 (fr) * 2016-06-17 2018-06-22 Universite De Tours Nouvelles proteines de fusion et leur application pour la preparation de vaccins
CN106226529B (zh) * 2016-07-14 2018-03-06 四川农业大学 脑多头蚴病的标志物ldh以及用于诊断脑多头蚴病的试剂盒
JP2019533993A (ja) * 2016-09-19 2019-11-28 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア ジカウイルスに対する新規ワクチンとジカウイルスに対する使用に向けたｄｎａ抗体構築物との組み合わせ
US20200362313A1 (en) * 2017-09-13 2020-11-19 Biontech Rna Pharmaceuticals Gmbh Method of enhancing rna expression in a cell
CN107828738A (zh) * 2017-11-28 2018-03-23 新乡医学院 一种dna甲基转移酶缺陷型cho细胞系及其制备方法及应用
WO2019143891A1 (en) * 2018-01-18 2019-07-25 The Penn State Research Foundation Mycobacterium avium subspecies paratuberculosis immunodiagnostic antigens, methods, and kits comprising same
CN110531077B (zh) * 2018-05-25 2023-07-07 荣昌生物制药(烟台)股份有限公司 间皮素免疫组化检测试剂盒
WO2020034097A1 (en) * 2018-08-14 2020-02-20 Wuxi Biologics (Shanghai) Co., Ltd. Transcriptional regulatory element and its use in enhancing the expression of exogenous protein
PT115073A (pt) 2018-10-09 2020-05-29 Inst De Biologia Molecular E Celular Ibmc Ácido nucleico para ativar a expressão e a produção de proteínas
KR102365464B1 (ko) * 2019-12-24 2022-02-22 강원대학교산학협력단 지카바이러스 재조합 서브유닛 백신의 개발 및 이의 제조방법
US11740240B2 (en) * 2020-07-20 2023-08-29 Bio-Rad Laboratories, Inc. Immunoassay for SARS-CoV-2 neutralizing antibodies and materials therefor
CN112359063A (zh) * 2020-11-04 2021-02-12 安徽环球基因科技有限公司 新冠重组rbd蛋白在昆虫细胞中的生产方法
CN114908119A (zh) * 2021-02-09 2022-08-16 佛山汉腾生物科技有限公司 一种提高重组蛋白表达量的方法
CN115976104A (zh) * 2023-01-03 2023-04-18 中国食品药品检定研究院 一种纯化蛋白的方法

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2125047B (en) 1982-08-09 1986-02-19 Ciba Geigy Ag Yeast hybrid vectors and their use for the production of polypeptides
EP0118551A1 (en) 1982-09-15 1984-09-19 Collaborative Research Inc. Production of interferon in yeast by use of invertase promoter
US4758512A (en) 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
HU206897B (en) 1985-10-25 1993-01-28 Zymogenetics Inc Process for utilizing bar-1 gen for selecting strange proteins
US5550043A (en) 1987-05-08 1996-08-27 Smithkline Beecham Corporation Expression of heterologous proteins in Drosophila cells
US5681713A (en) 1987-05-08 1997-10-28 Smithkline Beecham Corporation Expression of heterologous proteins in Drosophila cells
US5879926A (en) 1989-03-31 1999-03-09 Transgene S.A. Yeast strains for the production of mature heterologous proteins, especially hirudin
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
DK82893D0 (da) 1993-07-08 1993-07-08 Novo Nordisk As Peptid
FR2714830B1 (fr) 1994-01-10 1996-03-22 Rhone Poulenc Rorer Sa Composition contenant des acides nucléiques, préparation et utilisations.
FR2715847B1 (fr) 1994-02-08 1996-04-12 Rhone Poulenc Rorer Sa Composition contenant des acides nucléiques, préparation et utilisations.
FR2727679B1 (fr) 1994-12-05 1997-01-03 Rhone Poulenc Rorer Sa Nouveaux agents de transfection et leurs applications pharmaceutiques
FR2730637B1 (fr) 1995-02-17 1997-03-28 Rhone Poulenc Rorer Sa Composition pharmaceutique contenant des acides nucleiques, et ses utilisations
US5837520A (en) 1995-03-07 1998-11-17 Canji, Inc. Method of purification of viral vectors
US5919682A (en) 1995-08-24 1999-07-06 Board Of Regents, University Of Texas System Overproduction of neuronal nitric oxide synthase
AU3447097A (en) 1996-07-01 1998-01-21 Rhone-Poulenc Rorer S.A. Method for producing recombinant adenovirus
EP0931158A1 (en) 1996-09-06 1999-07-28 The Trustees Of The University Of Pennsylvania An inducible method for production of recombinant adeno-associated viruses utilizing t7 polymerase
WO1998017684A2 (en) * 1996-10-25 1998-04-30 Advanced Research & Technology Institute Dna sequences encoding fusions of dna repair proteins and uses thereof
DE69737107T2 (de) 1996-11-20 2007-07-12 Introgen Therapeutics Inc., Austin Ein verbessertes verfahren zur produktion und reinigung von adenoviralen vektoren
ES2283032T3 (es) 1996-12-13 2007-10-16 Schering Corporation Metodos para purificar virus.
FR2777909B1 (fr) 1998-04-24 2002-08-02 Pasteur Institut Utilisation de sequences d'adn de structure triplex pour le tranfert de sequences de nucleotides dans des cellules, vecteurs recombinants contenant ces sequences triplex
JP3864610B2 (ja) 1998-05-21 2007-01-10 旭硝子株式会社 水分散型撥水撥油剤組成物およびその製造方法
US7029909B1 (en) 1998-11-20 2006-04-18 Fuso Pharmaceutical Industries, Ltd. Protein expression vector and utilization thereof
JP2003523169A (ja) 1998-12-31 2003-08-05 アバンテイス・フアルマ・エス・アー ウイルス粒子の分離法
WO2000050573A1 (fr) 1999-02-22 2000-08-31 Transgene S.A. Procede d'obtention d'une preparation virale purifiee
DK1092779T3 (da) 1999-10-11 2010-02-15 Pasteur Institut Lentiviirusvektorer til fremstilling af immunterapeutiske præparater
ATE394496T1 (de) * 2000-03-24 2008-05-15 Genencor Int Herstellung von sekretierten proteinen durch rekombinante eukaryotische zellen
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US6682507B2 (en) 2002-02-20 2004-01-27 Doug's Kangaroo Pouch, Llc User wearable device having sterile environment for connecting peritoneal dialysis tubes
CN1720332A (zh) 2002-10-03 2006-01-11 洛桑生态综合技术联合公司 O6－烷基鸟嘌呤－dna烷基转移酶的底物
EP1546370A1 (en) * 2002-10-03 2005-06-29 Ecole Polytechnique Federale De Lausanne (Epfl) Protein labelling with o6-alkylguanine-dna alkyltransferase
JP2007526282A (ja) 2004-03-02 2007-09-13 ウペエフエル・エコル・ポリテクニック・フェデラル・ドゥ・ローザンヌ Ｏ６−アルキルグアニンｄｎａアルキルトランスフェラーゼ特異的基質
WO2006114409A1 (en) 2005-04-27 2006-11-02 Covalys Biosciences Ag Pyrimidines reacting with o6-alkylguanine-dna alkyltransferase
JP2009508470A (ja) * 2005-07-21 2009-03-05 アボット・ラボラトリーズ Ｓｏｒｆ構築物並びにポリタンパク質、プロタンパク質及びタンパク質分解による方法を含む複数の遺伝子発現
US20100196889A1 (en) * 2006-11-13 2010-08-05 Bankaitis-Davis Danute M Gene Expression Profiling for Identification, Monitoring and Treatment of Colorectal Cancer
EP2125868B1 (en) 2007-02-28 2015-06-10 The Govt. Of U.S.A. As Represented By The Secretary Of The Department Of Health And Human Services Brachyury polypeptides and methods for use
WO2009013359A2 (en) 2007-07-25 2009-01-29 Fraunhofer Gesellschaft Zur Förderung Der Angewandten Forschung E. V. Self coupling recombinant antibody fusion proteins
FR2934684B1 (fr) 2008-07-31 2012-11-16 Cis Bio Int Methode de detection de l'internalisation de proteines membranaires.
CN107090441A (zh) * 2010-12-09 2017-08-25 巴斯德研究所 用于获得高产量重组蛋白表达的基于mgmt的方法
JP6215223B2 (ja) * 2011-12-09 2017-10-18 アンスティテュ・パストゥール マルチプレックス免疫スクリーニングアッセイ

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CN103476928A (zh) 2013-12-25
EP3202897A1 (en) 2017-08-09
EP2649178B1 (en) 2017-03-15
US20170088843A1 (en) 2017-03-30
CN103476928B (zh) 2017-03-29
WO2012076715A1 (en) 2012-06-14
CN105821078A (zh) 2016-08-03
US9546380B2 (en) 2017-01-17
CA2819552C (en) 2023-09-26
ES2627117T3 (es) 2017-07-26
US20160017367A1 (en) 2016-01-21
JP6316856B2 (ja) 2018-04-25
EP2649178A1 (en) 2013-10-16
JP5940554B2 (ja) 2016-06-29
US20130309747A1 (en) 2013-11-21
CN107090441A (zh) 2017-08-25
US9109219B2 (en) 2015-08-18
US10017769B2 (en) 2018-07-10
BR112013014457A8 (pt) 2017-12-19
CA2819552A1 (en) 2012-06-14
EP3202897B1 (en) 2020-04-15
JP2016154543A (ja) 2016-09-01
EP2649178B8 (en) 2017-08-30
JP2014502163A (ja) 2014-01-30

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