BR112013011426A2 - método para purificação de material lipídico - Google Patents

método para purificação de material lipídico

Info

Publication number
BR112013011426A2
BR112013011426A2 BR112013011426A BR112013011426A BR112013011426A2 BR 112013011426 A2 BR112013011426 A2 BR 112013011426A2 BR 112013011426 A BR112013011426 A BR 112013011426A BR 112013011426 A BR112013011426 A BR 112013011426A BR 112013011426 A2 BR112013011426 A2 BR 112013011426A2
Authority
BR
Brazil
Prior art keywords
purification
lipid material
lipid
Prior art date
Application number
BR112013011426A
Other languages
English (en)
Inventor
Annika Malm
Mervi Hujanen
Reijo Tanner
Original Assignee
Neste Oil Oyj
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neste Oil Oyj filed Critical Neste Oil Oyj
Publication of BR112013011426A2 publication Critical patent/BR112013011426A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00Production of fats or fatty oils from raw materials
    • C11B1/10Production of fats or fatty oils from raw materials by extracting
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11BPRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00Refining fats or fatty oils
    • C11B3/006Refining fats or fatty oils by extraction
    • CCHEMISTRY; METALLURGY
    • C10PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10LFUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00Liquid carbonaceous fuels
    • C10L1/02Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • CCHEMISTRY; METALLURGY
    • C10PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10LFUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00Liquid carbonaceous fuels
    • C10L1/10Liquid carbonaceous fuels containing additives
    • C10L1/14Organic compounds
    • C10L1/18Organic compounds containing oxygen
    • CCHEMISTRY; METALLURGY
    • C10PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10LFUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G, C10K; LIQUEFIED PETROLEUM GAS; ADDING MATERIALS TO FUELS OR FIRES TO REDUCE SMOKE OR UNDESIRABLE DEPOSITS OR TO FACILITATE SOOT REMOVAL; FIRELIGHTERS
    • C10L1/00Liquid carbonaceous fuels
    • C10L1/10Liquid carbonaceous fuels containing additives
    • C10L1/14Organic compounds
    • C10L1/18Organic compounds containing oxygen
    • C10L1/1802Organic compounds containing oxygen natural products, e.g. waxes, extracts, fatty oils
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11CFATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C3/00Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Emergency Medicine (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Extraction Or Liquid Replacement (AREA)
BR112013011426A 2010-11-08 2011-11-08 método para purificação de material lipídico BR112013011426A2 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US41114510P 2010-11-08 2010-11-08
EP10190309.4A EP2450425B1 (en) 2010-11-08 2010-11-08 A method for lipid extraction from biomass
US13/117,185 US8648210B2 (en) 2010-11-08 2011-05-27 Method for purifying lipid material
EP11167858.7A EP2450426B1 (en) 2010-11-08 2011-05-27 A method for purifying lipid material
PCT/FI2011/050986 WO2012062963A1 (en) 2010-11-08 2011-11-08 A method for purifying lipid material

Publications (1)

Publication Number Publication Date
BR112013011426A2 true BR112013011426A2 (pt) 2016-08-02

Family

ID=43640686

Family Applications (2)

Application Number Title Priority Date Filing Date
BR112013011424-0A BR112013011424B1 (pt) 2010-11-08 2011-11-07 Método para extração de lipídeos a partir da biomassa
BR112013011426A BR112013011426A2 (pt) 2010-11-08 2011-11-08 método para purificação de material lipídico

Family Applications Before (1)

Application Number Title Priority Date Filing Date
BR112013011424-0A BR112013011424B1 (pt) 2010-11-08 2011-11-07 Método para extração de lipídeos a partir da biomassa

Country Status (16)

Country Link
US (2) US8648210B2 (pt)
EP (2) EP2450425B1 (pt)
JP (1) JP5893636B2 (pt)
KR (1) KR101990877B1 (pt)
CN (2) CN103328620B (pt)
AU (1) AU2011327956B2 (pt)
BR (2) BR112013011424B1 (pt)
CA (2) CA2817185C (pt)
DK (1) DK2450425T3 (pt)
ES (2) ES2490619T3 (pt)
HK (2) HK1187946A1 (pt)
IL (1) IL225981A (pt)
MX (1) MX2013005142A (pt)
PT (1) PT2450425E (pt)
SG (1) SG190153A1 (pt)
WO (2) WO2012062962A1 (pt)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2675517T3 (es) 2000-01-19 2018-07-11 Dsm Ip Assets B.V. Proceso de extracción sin solvente
US11247964B2 (en) 2009-05-01 2022-02-15 Rrip, Llc Method of processing phospholipid based lipid materials
US8308951B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Extraction of proteins by a two solvent method
EP2555633B1 (en) 2010-04-06 2014-06-11 Heliae Development LLC Selective extraction of proteins from freshwater or saltwater algae
US8211308B2 (en) * 2010-04-06 2012-07-03 Heliae Development, Llc Extraction of polar lipids by a two solvent method
US8115022B2 (en) 2010-04-06 2012-02-14 Heliae Development, Llc Methods of producing biofuels, chlorophylls and carotenoids
US8273248B1 (en) 2010-04-06 2012-09-25 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
US8313648B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Methods of and systems for producing biofuels from algal oil
US8475660B2 (en) 2010-04-06 2013-07-02 Heliae Development, Llc Extraction of polar lipids by a two solvent method
EP3617318A1 (en) 2010-06-01 2020-03-04 DSM IP Assets B.V. Extraction of lipid from cells and products therefrom
WO2013075116A2 (en) 2011-11-17 2013-05-23 Heliae Development, Llc Omega 7 rich compositions and methods of isolating omega 7 fatty acids
US8722911B2 (en) 2012-06-20 2014-05-13 Valicor, Inc. Process and method for improving the water reuse, energy efficiency, fermentation, and products of an ethanol fermentation plant
US9328310B1 (en) 2012-07-06 2016-05-03 Arrowhead Center, Inc. Subcritical water extraction of lipids from wet algal biomass
KR20150036502A (ko) 2012-07-13 2015-04-07 칼리스타, 인코포레이티드 바이오리파이너리 시스템, 이의 방법 및 조성물
SG11201506290RA (en) * 2013-03-13 2015-09-29 Sanofi Aventis Deutschland Production of squalene and/or sterol from cell suspensions of fermented yeast
WO2014152830A1 (en) 2013-03-14 2014-09-25 The University Of Wyoming Research Corporation Methods and systems for biological coal-to-biofuels and bioproducts
EP2970859A4 (en) 2013-03-14 2017-07-19 The University Of Wyoming Research Corporation Conversion of carbon dioxide utilizing chemoautotrophic microorganisms systems and methods
CN104059776B (zh) * 2013-03-20 2017-04-26 中国石油化工股份有限公司 一种高杂质油脂的处理方法
DE102014005372B4 (de) 2013-04-09 2022-09-01 Hochschule Anhalt Verfahren zur Abtrennung von extrazellulären Lipiden aus Algen
BR112015031937B1 (pt) * 2013-06-25 2022-02-22 Shell Internationale Research Maatschappij B.V. Método para produzir ésteres de ácido graxo, composição empregada na obtenção de ésteres de ácido graxo, e, composição remanescente da obtenção de ésteres de ácido graxo
CN105829539B (zh) 2013-12-20 2023-11-10 帝斯曼营养品股份公司 从微生物中回收油的方法
AU2014369045B2 (en) 2013-12-20 2020-02-27 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
KR102426987B1 (ko) 2013-12-20 2022-07-28 디에스엠 아이피 어셋츠 비.브이. 미생물 세포로부터의 미생물 오일의 수득 방법
ES2749002T3 (es) * 2013-12-20 2020-03-18 Mara Renewables Corp Métodos de recuperación de aceite sobre microorganismos
AU2014369042B2 (en) 2013-12-20 2020-04-30 Dsm Ip Assets B.V. Processes for obtaining microbial oil from microbial cells
SG11201605009RA (en) 2013-12-20 2016-07-28 Dsm Ip Assets Bv Processes for obtaining microbial oil from microbial cells
US20170119831A1 (en) * 2014-06-17 2017-05-04 Neste Oyj Use of hydrothermally treated biomass as pathogen binder
US10597601B2 (en) 2014-06-17 2020-03-24 Neste Oyj Method for recovering lipids from microbial biomass
EP3157538B1 (en) * 2014-06-17 2019-05-22 Neste Oyj Use of hydrothermally treated biomass as mycotoxin binder
FI128345B (en) * 2016-08-05 2020-03-31 Neste Oyj Method for cleaning the feed
FI128343B (en) * 2016-09-30 2020-03-31 Neste Oyj Process for the purification of lipid material
CN108034679B (zh) * 2017-11-23 2021-08-10 浙江海洋大学 一种从微藻中提取生物表面活性剂的方法
FI129178B (en) * 2017-12-29 2021-08-31 Neste Oyj Purification of biomass-based lipid material
FI129367B (en) * 2018-12-28 2021-12-31 Neste Oyj METHOD FOR HANDLING LIPID - CONTAINING MATERIALS
KR102395099B1 (ko) * 2019-08-02 2022-05-13 주식회사 청담 탈산 장치 및 이를 이용하는 탈산유 제조 방법
KR102395100B1 (ko) * 2019-10-30 2022-05-13 주식회사 청담 기능성 유지 제조 방법
WO2021213991A1 (en) 2020-04-20 2021-10-28 N.V. Desmet Ballestra Engineering S.A. Versatile method for purifying glyceridic materials
US11993752B1 (en) 2022-12-21 2024-05-28 Neste Oyj Process for manufacturing of renewable hydrocarbons from renewable feedstock comprising phosphorus as an impurity

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL57712A (en) * 1979-07-03 1984-02-29 Yissum Res Dev Co Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product
CA1157883A (en) * 1981-01-26 1983-11-29 United Oilseed Products Ltd. Chemical degumming of crude vegetable oils
JPS60238395A (ja) * 1984-05-14 1985-11-27 旭化成株式会社 植物油の精製法
JPS6244170A (ja) 1985-08-19 1987-02-26 Agency Of Ind Science & Technol モルテイエレラ属糸状菌体の超臨界流体による抽出方法
SE501697C2 (sv) * 1993-02-11 1995-04-24 Svenska Mejeriernas Riksforeni Förfarande för utvinning av sfingomyelin
DE4407933A1 (de) * 1993-03-15 1994-09-22 Sueddeutsche Kalkstickstoff Verfahren zur Gewinnung von Lipidfraktionen aus pulverförmigen Eiprodukten
US6063946A (en) * 1996-01-26 2000-05-16 Eastman Chemical Company Process for the isolation of polyunsaturated fatty acids and esters thereof from complex mixtures which contain sterols and phosphorus compounds
SE509358C2 (sv) * 1996-07-05 1999-01-18 Karlshamns Crushing & Feed Ab Användning av vattenånga för framställning av glyceridolja med låg halt av icke-hydratiserbara fosfatider
US6166231A (en) 1998-12-15 2000-12-26 Martek Biosciences Corporation Two phase extraction of oil from biomass
US6127560A (en) 1998-12-29 2000-10-03 West Central Cooperative Method for preparing a lower alkyl ester product from vegetable oil
ATE390190T1 (de) * 2001-11-13 2008-04-15 Metanomics Gmbh & Co Kgaa Verfahren zur extraktion von inhaltsstoffen aus organischem material
CA2469647C (en) * 2001-12-12 2011-02-15 Daniel G. Dueppen Extraction and winterization of lipids from oilseed and microbial sources
FR2852602B1 (fr) 2003-03-17 2007-08-10 Inst Francais Du Petrole Procede d'alcoolyse d'huiles acides d'origine vegetale ou animale
DE102004062141A1 (de) * 2004-12-23 2006-07-06 Nutrinova Nutrition Specialties & Food Ingredients Gmbh Verfahren zur Herstellung eines Rohöls aus Gemischen von Mikroorganismen und Pflanzen, das so hergestellte Öl sowie die spezifischen Verwendungen des so hergestellten und gegebenenfalls zusätzlich raffinierten Öls
AU2007203074A1 (en) * 2005-08-09 2007-07-19 Sqc Pty Ltd Extraction process-diesel fuel from hydrocarbon rich biomass
US8192774B2 (en) 2006-03-14 2012-06-05 Oilseeds Biorefinery Corporation Oil extraction
GR1006009B (el) * 2006-07-18 2008-07-29 Ευστρατιος Χατζηεμμανουηλ Μεθοδος εξευγενισμου φυτικου ελαιου και προσθετο για το σκοπο αυτο, οπως επισης και η χρησιμοποιησητους.
WO2008034109A1 (en) 2006-09-14 2008-03-20 Biofuelbox Corporation Methods of robust and efficient conversion of cellular lipids to biofuels
CN1923960A (zh) * 2006-10-08 2007-03-07 清华大学 微生物发酵油脂及其用于制备生物柴油的方法
DE602007011667D1 (de) 2006-12-01 2011-02-10 Univ North Carolina State Verfahren zur umwandlung von biomasse in treibstoff
NZ578234A (en) * 2007-05-02 2012-12-21 Ouro Fino Participacoes E Empreendimentos S A Process to produce biodiesel and/or fuel oil
NZ598199A (en) * 2007-09-12 2013-12-20 Martek Biosciences Corp Biological oils and production and uses thereof
US8017819B2 (en) 2008-04-25 2011-09-13 Conocophillips Company Thermal treatment of triglycerides
ITMI20081203A1 (it) 2008-06-30 2010-01-01 Eni Spa Procedimento per l'estrazione di acidi grassi da biomassa algale
IT1392810B1 (it) * 2009-02-04 2012-03-23 Eni Spa Procedimento per l'estrazione di lipidi da biomassa algale
EP2406361B1 (en) 2009-03-11 2021-01-20 Swedish Oat Fiber AB Method for separating neutral and polar lipids and an oil rich in polar lipids
US20110047866A1 (en) 2009-08-31 2011-03-03 Conocophillips Company Removal of impurities from oils and/or fats
MX2012004676A (es) * 2009-10-22 2012-10-05 Univ Illinois Procesamiento hidrotermal (htp) de algas cultivadas en corrientes residuales de htp.

Also Published As

Publication number Publication date
DK2450425T3 (da) 2014-07-07
PT2450425E (pt) 2014-07-24
CN103328620B (zh) 2014-08-27
KR101990877B1 (ko) 2019-06-19
SG190153A1 (en) 2013-07-31
KR20140007065A (ko) 2014-01-16
EP2450426B1 (en) 2017-07-12
CA2817185A1 (en) 2012-05-18
CN103270147B (zh) 2016-01-27
EP2450426A1 (en) 2012-05-09
IL225981A0 (en) 2013-06-27
AU2011327956B2 (en) 2015-11-12
IL225981A (en) 2017-05-29
AU2011327956A1 (en) 2013-05-23
JP5893636B2 (ja) 2016-03-23
US20120110898A1 (en) 2012-05-10
AU2011327957A1 (en) 2013-05-23
EP2450425B1 (en) 2014-05-14
BR112013011424A2 (pt) 2016-08-02
CA2817185C (en) 2018-11-06
US8648210B2 (en) 2014-02-11
US20120116103A1 (en) 2012-05-10
ES2641494T3 (es) 2017-11-10
HK1187946A1 (en) 2014-04-17
CA2817186C (en) 2018-01-02
JP2013543910A (ja) 2013-12-09
WO2012062963A1 (en) 2012-05-18
MX2013005142A (es) 2013-08-01
HK1189022A1 (zh) 2014-05-23
CN103270147A (zh) 2013-08-28
CA2817186A1 (en) 2012-05-18
EP2450425A1 (en) 2012-05-09
BR112013011424B1 (pt) 2021-08-10
CN103328620A (zh) 2013-09-25
US8845765B2 (en) 2014-09-30
WO2012062962A1 (en) 2012-05-18
ES2490619T3 (es) 2014-09-04

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B06T Formal requirements before examination [chapter 6.20 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]