BR112013010585A2 - métodos de mpcr para análise de sequências de repetição - Google Patents

métodos de mpcr para análise de sequências de repetição

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Publication number
BR112013010585A2
BR112013010585A2 BR112013010585A BR112013010585A BR112013010585A2 BR 112013010585 A2 BR112013010585 A2 BR 112013010585A2 BR 112013010585 A BR112013010585 A BR 112013010585A BR 112013010585 A BR112013010585 A BR 112013010585A BR 112013010585 A2 BR112013010585 A2 BR 112013010585A2
Authority
BR
Brazil
Prior art keywords
methods
parsing
repeat sequences
mpcr
rich
Prior art date
Application number
BR112013010585A
Other languages
English (en)
Other versions
BR112013010585B1 (pt
Inventor
Andrew Hadd
Gary J Latham
Liangjing Chen
Ru Cao
Sachin Sah
Original Assignee
Asuragen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asuragen Inc filed Critical Asuragen Inc
Publication of BR112013010585A2 publication Critical patent/BR112013010585A2/pt
Publication of BR112013010585B1 publication Critical patent/BR112013010585B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/125—Electrophoretic separation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

métodos de mpcr para análise de sequências de repetição. são providos métodos para determinação do estado de metilação de moldes ricos em gc. os métodos incluem uso de padrões de referência gc que permitem caracterização simultânea de estado de metilação e comprimento de repetição de cgc. os métodos são úteis para detecção de genótipos associados com repetições ricas em gc, incluindo síndrome do x frágil.
BR112013010585-2A 2010-10-29 2011-10-28 método de caracterização de um locus de fmr1 ou um locus de frm2 em uma amostra de dna e método de análise de um locus fmr1 ou um locus fmr2 de uma amostra de dna humana BR112013010585B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40836710P 2010-10-29 2010-10-29
US61/408,367 2010-10-29
PCT/US2011/058453 WO2012058633A1 (en) 2010-10-29 2011-10-28 mPCR METHODS FOR ANALYZING REPEAT SEQUENCES

Publications (2)

Publication Number Publication Date
BR112013010585A2 true BR112013010585A2 (pt) 2016-08-09
BR112013010585B1 BR112013010585B1 (pt) 2020-12-01

Family

ID=44971100

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013010585-2A BR112013010585B1 (pt) 2010-10-29 2011-10-28 método de caracterização de um locus de fmr1 ou um locus de frm2 em uma amostra de dna e método de análise de um locus fmr1 ou um locus fmr2 de uma amostra de dna humana

Country Status (10)

Country Link
US (1) US9777330B2 (pt)
EP (1) EP2633073B1 (pt)
JP (1) JP6081366B2 (pt)
KR (1) KR101911966B1 (pt)
CN (2) CN103282517B (pt)
AU (1) AU2011320359B2 (pt)
BR (1) BR112013010585B1 (pt)
IL (1) IL225833A0 (pt)
WO (1) WO2012058633A1 (pt)
ZA (1) ZA201303478B (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2013266341B2 (en) * 2012-05-22 2019-01-03 The Johns Hopkins University A quantitative multiplex methylation specific PCR method- cMethDNA, reagents, and its use
US9371560B2 (en) 2012-07-20 2016-06-21 Asuragen, Inc. Comprehensive FMR1 genotyping
WO2014210559A1 (en) * 2013-06-28 2014-12-31 Life Technologies Corporation Methods and systems for visualizing data quality
CN104450911B (zh) * 2014-12-09 2017-05-17 上海五色石医学研究有限公司 一种fra x综合症相关基因fmr1检测试剂盒及其应用
CN104498607A (zh) * 2014-12-17 2015-04-08 绍兴华因生物科技有限公司 基于甲基化水平检测脆性x染色体综合征的定量比较法及其引物
BR112018008546A2 (pt) 2015-11-03 2018-10-30 Asuragen Inc métodos para detecção de tamanho de ácido nucleico de sequências de repetição
CN107523621A (zh) * 2017-09-04 2017-12-29 广州市达瑞生物技术股份有限公司 一种用于检测脆性x综合征的引物组及其试剂盒
CN108531576A (zh) * 2018-04-12 2018-09-14 北京信诺佰世医学检验所有限公司 检测脆性x染色体综合征的试剂盒和系统
CN108546755A (zh) * 2018-05-09 2018-09-18 广州市达瑞生物技术股份有限公司 用于脆性x综合征致病基因检测的校准品及其应用
DE102018213027A1 (de) * 2018-08-03 2020-02-06 Robert Bosch Gmbh Reaktionsgemisch, Verfahren und Kit zur Durchführung einer quantitativen Echtzeit-PCR
EP4097723A1 (en) 2020-01-30 2022-12-07 Laboratory Corporation of America Holdings Fragile x syndrome agg interruption genotyping

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
ES2159279T3 (es) * 1991-01-04 2001-10-01 Univ Washington Secuencias de dna relacionadas con el sindrome del cromosoma x fragil aislado.
US6180337B1 (en) * 1991-05-24 2001-01-30 Baylor College Of Medicine Diagnosis of the fragile X syndrome
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5863727A (en) 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5847162A (en) 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
SE506700C2 (sv) 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
US6008379A (en) 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US5936087A (en) 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
US6140054A (en) 1998-09-30 2000-10-31 University Of Utah Research Foundation Multiplex genotyping using fluorescent hybridization probes
US6140500A (en) 1999-09-03 2000-10-31 Pe Corporation Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for their use
US6191278B1 (en) 1999-11-03 2001-02-20 Pe Corporation Water-soluble rhodamine dyes and conjugates thereof
EP1438416A4 (en) * 2001-09-26 2007-09-05 Epigenx Pharmaceutical Inc ASSAYS FOR CHANGES TO THE METHYLATION OF DNA
JP4189495B2 (ja) * 2004-08-02 2008-12-03 国立大学法人群馬大学 ゲノムｄｎａのメチル化検出方法
CA2629008A1 (en) * 2005-11-08 2007-09-13 Euclid Diagnostics Llc Materials and methods for assaying for methylation of cpg islands associated with genes in the evaluation of cancer
CN101421410A (zh) * 2006-03-06 2009-04-29 纽约市哥伦比亚大学托管会 从混合的胎儿-母体来源中特异性扩增胎儿dna序列
US7855053B2 (en) 2006-07-19 2010-12-21 The Regents Of The University Of California Methods for detecting the presence of expanded CGG repeats in the FMR1 gene 5′ untranslated region
JP2009278867A (ja) * 2006-09-05 2009-12-03 Olympus Corp Ｄｎａメチル化阻害作用の判定方法
US8409805B2 (en) * 2009-02-13 2013-04-02 Asuragen, Inc. Method of amplification of GC-rich DNA templates
WO2010110835A1 (en) 2009-03-24 2010-09-30 Asuragen, Inc. Pcr methods for characterizing the 5' untranslated region of the fmr1 and fmr2 genes
US20100248239A1 (en) * 2009-03-24 2010-09-30 Mayo Foundation For Medical Education And Research Methods and materials for detecting fragile x mutations
WO2011070441A2 (en) * 2009-12-11 2011-06-16 Adam Wasserstrom Categorization of dna samples
EP2539464B1 (en) * 2010-02-23 2016-11-16 Illumina, Inc. Amplification methods to minimise sequence specific bias

Also Published As

Publication number Publication date
JP6081366B2 (ja) 2017-02-15
JP2013540452A (ja) 2013-11-07
IL225833A0 (en) 2013-06-27
CN106868107A (zh) 2017-06-20
CN103282517B (zh) 2018-11-13
EP2633073A1 (en) 2013-09-04
CN103282517A (zh) 2013-09-04
EP2633073B1 (en) 2017-04-26
AU2011320359A1 (en) 2013-05-02
ZA201303478B (en) 2018-12-19
BR112013010585B1 (pt) 2020-12-01
US20120107824A1 (en) 2012-05-03
AU2011320359B2 (en) 2015-07-16
KR20140049960A (ko) 2014-04-28
WO2012058633A1 (en) 2012-05-03
KR101911966B1 (ko) 2018-10-25
US9777330B2 (en) 2017-10-03

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 28/10/2011, OBSERVADAS AS CONDICOES LEGAIS.