BR112012022006A2 - um processo para a síntese enzimática de alquil ésteres de ácido graxo - Google Patents

um processo para a síntese enzimática de alquil ésteres de ácido graxo

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Publication number
BR112012022006A2
BR112012022006A2 BR112012022006A BR112012022006A BR112012022006A2 BR 112012022006 A2 BR112012022006 A2 BR 112012022006A2 BR 112012022006 A BR112012022006 A BR 112012022006A BR 112012022006 A BR112012022006 A BR 112012022006A BR 112012022006 A2 BR112012022006 A2 BR 112012022006A2
Authority
BR
Brazil
Prior art keywords
fatty acid
acid esters
alkyl
alkyl fatty
enzymatic synthesis
Prior art date
Application number
BR112012022006A
Other languages
English (en)
Other versions
BR112012022006B1 (pt
Inventor
Sobhi Basheer
Maisa Haj
Ahmad Hindawi
Emad Masoud
Usama Mohsen
Doaa Shehadeh
Original Assignee
Trans Bio Diesel Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Trans Bio Diesel Ltd filed Critical Trans Bio Diesel Ltd
Publication of BR112012022006A2 publication Critical patent/BR112012022006A2/pt
Publication of BR112012022006B1 publication Critical patent/BR112012022006B1/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11C—FATTY ACIDS FROM FATS, OILS OR WAXES; CANDLES; FATS, OILS OR FATTY ACIDS BY CHEMICAL MODIFICATION OF FATS, OILS, OR FATTY ACIDS OBTAINED THEREFROM
    • C11C3/00—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom
    • C11C3/003—Fats, oils, or fatty acids by chemical modification of fats, oils, or fatty acids obtained therefrom by esterification of fatty acids with alcohols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/091—Phenol resins; Amino resins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

um processo para a síntese enzimática de alquil ésteres de ácido graxo. são descritos um processo enzimático em lote ou contínuo para a produção de alquil ésteres de ácido graxo para uso em indústria de biocombustíveis, alimentos e detergente, e um sistema para o mesmo. o processo utiliza enzimas imobilizadas sobre uma resina hidrofóbica mistura com uma fonte de ácido graxo e um álcool ou doador de álcool na presença de um tampão aquoso alcalino ou levemente alcalino, ou na presença de solução de água. o processo de produção para alquil ésteres de ácido graxo é executado pela transesterificação ou esterificação simultaneamente ou sequencialmente. a atividade biocatalisadora é mantida sem perdas de atividade significativas em múltiplos usos e também evita o acúmulo evita o acúmulo de subprodutos de glicerol e água ou outros compostos hidrofilicos no biocatalisador.
BR112012022006-3A 2010-03-01 2011-02-02 um processo para a síntese enzimática de alquil ésteres de ácido graxo BR112012022006B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US30912210P 2010-03-01 2010-03-01
US61/309,122 2010-03-01
PCT/IL2011/000121 WO2011107977A1 (en) 2010-03-01 2011-02-02 A process for the enzymatic synthesis of fatty acid alkyl esters

Publications (2)

Publication Number Publication Date
BR112012022006A2 true BR112012022006A2 (pt) 2019-09-24
BR112012022006B1 BR112012022006B1 (pt) 2021-02-23

Family

ID=44150462

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012022006-3A BR112012022006B1 (pt) 2010-03-01 2011-02-02 um processo para a síntese enzimática de alquil ésteres de ácido graxo

Country Status (14)

Country Link
EP (1) EP2542685B1 (pt)
JP (1) JP5554848B2 (pt)
KR (2) KR20130004286A (pt)
CN (1) CN102812128B (pt)
AR (1) AR080117A1 (pt)
AU (1) AU2011222439B2 (pt)
BR (1) BR112012022006B1 (pt)
CA (1) CA2791836C (pt)
DK (1) DK2542685T3 (pt)
ES (1) ES2462548T3 (pt)
MX (1) MX2012010056A (pt)
RU (1) RU2600879C2 (pt)
WO (1) WO2011107977A1 (pt)
ZA (1) ZA201206168B (pt)

Families Citing this family (25)

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US10000731B2 (en) 2010-03-01 2018-06-19 Trans Bio-Diesel Ltd. Enzymatic transesterification/esterification processes employing lipases immobilized on hydrophobic resins in the presence of water solutions
US8115022B2 (en) 2010-04-06 2012-02-14 Heliae Development, Llc Methods of producing biofuels, chlorophylls and carotenoids
CN102858988A (zh) 2010-04-06 2013-01-02 赫里开发公司 通过使用具有递减极性的溶剂从含油材料连续提取油和蛋白质材料
US8313648B2 (en) 2010-04-06 2012-11-20 Heliae Development, Llc Methods of and systems for producing biofuels from algal oil
US8475660B2 (en) 2010-04-06 2013-07-02 Heliae Development, Llc Extraction of polar lipids by a two solvent method
US8308951B1 (en) 2010-04-06 2012-11-13 Heliae Development, Llc Extraction of proteins by a two solvent method
US8273248B1 (en) 2010-04-06 2012-09-25 Heliae Development, Llc Extraction of neutral lipids by a two solvent method
AU2011376066B2 (en) * 2011-08-31 2015-11-26 Trans Bio-Diesel Ltd. Enzymatic transesterification with lipases immobilized on hydrophobic resins in water solutions
US9200236B2 (en) 2011-11-17 2015-12-01 Heliae Development, Llc Omega 7 rich compositions and methods of isolating omega 7 fatty acids
WO2013081446A1 (en) * 2011-12-02 2013-06-06 Universiti Malaya A process for producing biodiesel from natural oil and a system thereof
US20150353970A1 (en) 2012-12-31 2015-12-10 Trans Bio-Diesel Ltd. Enzymatic transesterification/esterification processing systems and processes employing lipases immobilzed on hydrophobic resins
CN103044260A (zh) * 2013-01-18 2013-04-17 武汉大学 一种制备碳酸甲基烷基酯的方法
KR101355141B1 (ko) * 2013-03-20 2014-01-29 주식회사 엠알아이 바이오디젤의 제조방법
KR101684455B1 (ko) * 2013-07-30 2016-12-08 한국생명공학연구원 고정화 효소를 이용한 연속식 바이오디젤 생산 반응기 및 바이오디젤 생산 방법
KR101511744B1 (ko) * 2014-04-28 2015-04-22 티케이엘 주식회사 효소순환공정을 이용한 세틸화된 지방산 복합물의 생산장치
CN113293183A (zh) * 2014-05-28 2021-08-24 诺维信公司 采用碱处理进行脂肪酸烷基酯生产
KR101590268B1 (ko) * 2014-09-16 2016-02-01 광운대학교 산학협력단 효소를 이용한 글리세롤 아세테이트의 제조 방법
US10435726B2 (en) 2014-12-19 2019-10-08 International Flora Technologies, Ltd. Processes and systems for catalytic manufacture of wax ester derivatives
US11248245B2 (en) 2014-12-19 2022-02-15 International Flora Technologies, Ltd. Processes and systems for catalytic manufacture of wax ester derivatives
CA2996591C (en) * 2015-08-31 2021-07-20 International Flora Technologies, Ltd. Processes and systems for catalytic manufacture of wax ester derivatives
CN106906256A (zh) * 2017-03-20 2017-06-30 威莱(广州)日用品有限公司 一种脂肪酸甲酯乙氧基化物的合成方法
KR102312069B1 (ko) * 2019-12-31 2021-10-13 주식회사 제이엘비 식물성 에스테르 오일이 함유된 천연화장품 조성물
KR102332935B1 (ko) * 2020-04-06 2021-11-30 한국조선해양 주식회사 바이오 연료 시스템 및 이를 포함하는 해양구조물
IL286349A (en) * 2021-09-13 2023-04-01 Univice M E Ltd Processes for the production of biodiesel from fatty waste
FR3126994A1 (fr) * 2021-09-14 2023-03-17 Gecco Procédé et installation pour la synthèse enzymatique d’un biodiésel à partir de lipides usagés

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Also Published As

Publication number Publication date
ES2462548T3 (es) 2014-05-23
AU2011222439B2 (en) 2013-07-18
CN102812128B (zh) 2017-09-12
CN102812128A (zh) 2012-12-05
EP2542685A1 (en) 2013-01-09
DK2542685T3 (da) 2014-06-23
AU2011222439A1 (en) 2012-09-06
JP5554848B2 (ja) 2014-07-23
ZA201206168B (en) 2014-01-29
JP2013520985A (ja) 2013-06-10
RU2600879C2 (ru) 2016-10-27
MX2012010056A (es) 2012-09-28
EP2542685B1 (en) 2014-03-26
CA2791836C (en) 2014-10-28
AR080117A1 (es) 2012-03-14
WO2011107977A1 (en) 2011-09-09
KR101858915B1 (ko) 2018-05-16
KR20130004286A (ko) 2013-01-09
KR20170104002A (ko) 2017-09-13
CA2791836A1 (en) 2011-09-09
RU2012141298A (ru) 2014-04-27
BR112012022006B1 (pt) 2021-02-23

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