BR0116669A - Método e sistema para preparação de amostras de tecido para exame histológico e patológico - Google Patents

Método e sistema para preparação de amostras de tecido para exame histológico e patológico

Info

Publication number
BR0116669A
BR0116669A BR0116669-7A BR0116669A BR0116669A BR 0116669 A BR0116669 A BR 0116669A BR 0116669 A BR0116669 A BR 0116669A BR 0116669 A BR0116669 A BR 0116669A
Authority
BR
Brazil
Prior art keywords
present
histological
biological material
coolant
freezing
Prior art date
Application number
BR0116669-7A
Other languages
English (en)
Inventor
Samuel D Prien
John Blanton
Brian Wood
Allan J Cassell
Original Assignee
Supachill Technologies Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Supachill Technologies Pty Ltd filed Critical Supachill Technologies Pty Ltd
Publication of BR0116669A publication Critical patent/BR0116669A/pt

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D31/00—Other cooling or freezing apparatus
    • F25D31/002—Liquid coolers, e.g. beverage cooler
    • F25D31/003—Liquid coolers, e.g. beverage cooler with immersed cooling element
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
    • A01N1/0257—Stationary or portable vessels generating cryogenic temperatures
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D2400/00—General features of, or devices for refrigerators, cold rooms, ice-boxes, or for cooling or freezing apparatus not covered by any other subclass
    • F25D2400/30—Quick freezing
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D25/00—Charging, supporting, and discharging the articles to be cooled
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Mechanical Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Combustion & Propulsion (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"MéTODO E SISTEMA PARA PREPARAçãO DE AMOSTRAS DE TECIDO PARA EXAME HISTOLóGICO E PATOLóGICO". Material biológico viável é criogenicamente preservado (criopreservação) por imersão do material em um tanque de fluido de resfriamento, e circulação do fluido de resfriamento acima do material em uma velocidade e temperatura predeterminadas substancialmente constantes para congelar o material. O material pode ser mergulhado diretamente no fluido de resfriamento sem preparação, ou preparado quimicamente antes do congelamento. Um método de acordo com a presente invenção congela o material biológico de forma rápida o suficiente para evitar a formação de cristais de gelo nas estruturas celulares (vitrificação) e permite que as amostras mantenham a estrutura anatómica e permaneçam bioquimicamente ativas após o descongelamento. A temperatura do fluido de resfriamento é preferivelmente entre -200<198>C e -30O<198>C, que é quente o suficiente para minimizar a formação de fraturas de stress e outros artefatos em membranas celulares devido a mudanças térmicas. Células congeladas que usam um método de acordo com a presente invenção mostrou ter um dano celular e intercelular significativamente menor que as células congeladas por outros métodos de criopreservação usados para técnicas patológicas e histológicas. Devido ao fato da presente invenção poder congelar material biológico de forma que o material seja vitrificado, a atividade bioquímica dentro da célula não é perdida após o congelamento e assim várias modalidades do presente método podem ser empregadas em um sistema para preparar material biológico para as novas técnicas de criopatologia e imunohistoquímica nas áreas de pesquisa e cuidados com pacientes.
BR0116669-7A 2001-01-02 2001-12-28 Método e sistema para preparação de amostras de tecido para exame histológico e patológico BR0116669A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25941801P 2001-01-02 2001-01-02
PCT/US2001/049595 WO2002076203A2 (en) 2001-01-02 2001-12-28 Method and system for preparing tissue samples for histological and pathological examination

Publications (1)

Publication Number Publication Date
BR0116669A true BR0116669A (pt) 2005-01-18

Family

ID=22984862

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0116669-7A BR0116669A (pt) 2001-01-02 2001-12-28 Método e sistema para preparação de amostras de tecido para exame histológico e patológico

Country Status (15)

Country Link
US (2) US6615592B2 (pt)
EP (1) EP1381841A2 (pt)
JP (1) JP2004537035A (pt)
KR (1) KR20040036683A (pt)
CN (1) CN1685210A (pt)
BR (1) BR0116669A (pt)
CA (1) CA2433356A1 (pt)
CZ (1) CZ20031811A3 (pt)
IL (1) IL156608A0 (pt)
MX (1) MXPA03006063A (pt)
NO (1) NO20033039L (pt)
NZ (1) NZ526793A (pt)
RU (1) RU2003124058A (pt)
WO (1) WO2002076203A2 (pt)
ZA (1) ZA200304949B (pt)

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MXPA03006063A (es) * 2001-01-02 2005-02-14 Supachill Technologies Pty Ltd Metodo y sistema para preparar muestras de tejidos para examenes histologicos y patologicos.
US20030096412A1 (en) * 2001-11-20 2003-05-22 Cassell Allan J. Method of preparing biological materials for cryopreservation using pre-chilled protectant
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CN101545839B (zh) * 2009-03-20 2011-06-22 西安交通大学 一种使用冷冻干燥技术对生物样品进行前处理的方法
DE102009061068B4 (de) 2009-04-17 2015-05-07 Leica Biosystems Nussloch Gmbh Verfahren zur Herstellung mikroskopierbarer Dünnschnitte von Gewebeproben
CN103201609A (zh) * 2010-09-21 2013-07-10 Fei公司 制备供电子显微镜和荧光显微镜观察的生物样品的方法
CN102692344A (zh) * 2012-05-21 2012-09-26 中国人民解放军第二军医大学 电刺激—毫秒级超低温快速冷冻方法及其装置
DE102012013680A1 (de) * 2012-07-11 2014-01-16 Euroimmun Medizinische Labordiagnostika Ag Vorrichtung sowie Verfahren zur Inkubation von Patientenproben
CN103190393B (zh) * 2013-04-09 2015-05-13 上海安久生物科技有限公司 一种生物样本玻璃化冷冻载体及其用途
CN103674678A (zh) * 2013-12-17 2014-03-26 电子科技大学 生物材料低温保存微通道芯片及装置
CN103954485B (zh) * 2014-04-17 2016-03-30 华北水利水电大学 一种实验用圆柱体冰样制作方法
WO2017099865A1 (en) 2015-12-07 2017-06-15 Coopersurgical, Inc. Low temperature specimen carriers and related methods
RU2769506C2 (ru) * 2016-08-15 2022-04-01 Ванесса Рисерч, Инк. Устройство для формирования и замораживания образцов ткани пациента или животного и способ формирования и замораживания образцов ткани пациента или животного
CN106370473A (zh) * 2016-11-22 2017-02-01 青岛啤酒股份有限公司 一种用于取样液体降温的取样器及其使用方法
CN108716395B (zh) * 2018-05-22 2019-09-17 中国石油大学(北京) 基于冰块压裂的可视化试验方法
AU2019385712A1 (en) * 2018-11-22 2021-06-10 Vitrafy Life Sciences Limited Method and apparatus for freezing of biological products
CN111413133A (zh) * 2020-04-20 2020-07-14 华中科技大学 一种冰冻切片机
AU2022343612A1 (en) * 2021-09-10 2024-03-21 Elephas Biosciences Corporation Tissue cutting system and method

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Also Published As

Publication number Publication date
MXPA03006063A (es) 2005-02-14
RU2003124058A (ru) 2005-01-10
ZA200304949B (en) 2004-08-25
EP1381841A2 (en) 2004-01-21
WO2002076203A2 (en) 2002-10-03
US20040083741A1 (en) 2004-05-06
JP2004537035A (ja) 2004-12-09
CN1685210A (zh) 2005-10-19
WO2002076203A9 (en) 2004-04-01
NO20033039D0 (no) 2003-07-02
NO20033039L (no) 2003-07-02
US20020100284A1 (en) 2002-08-01
US6615592B2 (en) 2003-09-09
CZ20031811A3 (cs) 2004-02-18
WO2002076203A3 (en) 2003-11-13
IL156608A0 (en) 2004-01-04
KR20040036683A (ko) 2004-04-30
CA2433356A1 (en) 2002-10-03
NZ526793A (en) 2006-02-24

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