AU780319B2 - Simultaneous isolation and quantitation of DNA - Google Patents

Simultaneous isolation and quantitation of DNA

Info

Publication number
AU780319B2
AU780319B2 AU67895/00A AU6789500A AU780319B2 AU 780319 B2 AU780319 B2 AU 780319B2 AU 67895/00 A AU67895/00 A AU 67895/00A AU 6789500 A AU6789500 A AU 6789500A AU 780319 B2 AU780319 B2 AU 780319B2
Authority
AU
Australia
Prior art keywords
target material
dna target
dna
particles
magnetic particles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU67895/00A
Other languages
English (en)
Other versions
AU6789500A (en
Inventor
Rex M. Bitner
Steven J. Ekenberg
Daniel D. Kephart
Susan C. Koller
Craig E. Smith
Allan M. Tereba
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/377,986 external-priority patent/US6673631B1/en
Application filed by Promega Corp filed Critical Promega Corp
Publication of AU6789500A publication Critical patent/AU6789500A/en
Application granted granted Critical
Publication of AU780319B2 publication Critical patent/AU780319B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU67895/00A 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of DNA Ceased AU780319B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/377,986 US6673631B1 (en) 1997-01-21 1999-08-20 Simultaneous isolation and quantitation of DNA
US09/377986 1999-08-20
PCT/US2000/022827 WO2001014590A2 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of dna

Publications (2)

Publication Number Publication Date
AU6789500A AU6789500A (en) 2001-03-19
AU780319B2 true AU780319B2 (en) 2005-03-17

Family

ID=23491269

Family Applications (1)

Application Number Title Priority Date Filing Date
AU67895/00A Ceased AU780319B2 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of DNA

Country Status (8)

Country Link
EP (1) EP1204741B1 (enExample)
JP (1) JP4425513B2 (enExample)
CN (1) CN1370230A (enExample)
AT (1) ATE280225T1 (enExample)
AU (1) AU780319B2 (enExample)
CA (1) CA2379503A1 (enExample)
DE (1) DE60015148T2 (enExample)
WO (1) WO2001014590A2 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60234464D1 (de) 2001-11-28 2009-12-31 Applied Biosystems Llc Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung
ES2294287T5 (es) 2002-01-08 2015-10-27 F. Hoffmann-La Roche Ag Empleo de un material de sílice en una reacción de amplificación
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
EP1621890A1 (en) * 2004-07-26 2006-02-01 bioMerieux B.V. Device and method for separating, mixing and concentrating magnetic particles with a fluid and use thereof in purification methods
EP1629887B1 (de) * 2004-07-29 2007-04-11 Qiagen GmbH Monolithische Formkörper zur Aufreinigung und Trennung von Biopolymeren
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
US20090277790A1 (en) 2006-04-14 2009-11-12 Nec Corporation Individual identification method and apparatus
JP2009065900A (ja) * 2007-09-13 2009-04-02 Olympus Corp 標的rnaの解析方法及び保存方法
WO2009068584A1 (en) * 2007-11-26 2009-06-04 Atonomics A/S Integrated separation and detection cartridge with means and method for increasing signal to noise ratio
DE102008047790A1 (de) * 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
WO2011077333A1 (en) * 2009-12-23 2011-06-30 Koninklijke Philips Electronics N.V. Analyte measurement apparatus and method
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
CN101818143A (zh) * 2010-04-28 2010-09-01 长春市博坤生物科技有限公司 基于二氧化硅磁性复合微粒的超低量dna提取试剂盒及方法
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
US9725754B2 (en) 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
JP6169489B2 (ja) 2010-07-29 2017-07-26 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 一般的な試料調製
BR112014018187A8 (pt) * 2012-01-25 2017-07-11 Gencell Biosystems Ltd Métodos para manusear líquidos de amostra contendo partículas magnéticas e um líquido de encapsulação, bem como sistema de manuseio de líquido
AU2013240200B2 (en) * 2012-03-28 2016-10-20 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for the collection and isolation of nucleic acids from biological specimens
WO2014035090A1 (ko) * 2012-08-28 2014-03-06 주식회사 바이오큐브시스템 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
CN105603056B (zh) * 2014-11-21 2020-09-25 丰益(上海)生物技术研发中心有限公司 一种植物油脂掺杂检测的方法
CN104560952A (zh) * 2014-12-29 2015-04-29 福建师范大学 一种基于磁珠法快速提取革兰氏阳性菌基因组dna的方法
WO2016183292A1 (en) 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
JP2018526021A (ja) * 2015-09-11 2018-09-13 コーニング インコーポレイテッド 血液試料からの核酸精製のための組成物及び方法
US20190329244A1 (en) * 2017-01-04 2019-10-31 Agency For Science, Technology And Research Sieve-through vertical flow system for particle-based bioassays
CN109321634A (zh) * 2017-07-26 2019-02-12 上海之江生物科技股份有限公司 核酸均一化方法及其试剂盒和应用
WO2019035335A1 (ja) * 2017-08-18 2019-02-21 Agc株式会社 核酸の回収方法、核酸結合用担体および核酸回収キット
CN118284704A (zh) * 2021-11-23 2024-07-02 柏尔科学公司 用于核酸标准化的组合物和方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031840A1 (en) * 1997-01-21 1998-07-23 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031840A1 (en) * 1997-01-21 1998-07-23 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
ATE280225T1 (de) 2004-11-15
WO2001014590A3 (en) 2001-10-11
AU6789500A (en) 2001-03-19
JP4425513B2 (ja) 2010-03-03
CA2379503A1 (en) 2001-03-01
CN1370230A (zh) 2002-09-18
JP2003507049A (ja) 2003-02-25
EP1204741A2 (en) 2002-05-15
EP1204741B1 (en) 2004-10-20
WO2001014590A2 (en) 2001-03-01
DE60015148T2 (de) 2006-02-16
DE60015148D1 (de) 2004-11-25

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