JP4425513B2 - Ｄｎａの同時単離及び定量化 - Google Patents

Ｄｎａの同時単離及び定量化 Download PDF

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Publication number
JP4425513B2
JP4425513B2 JP2001518457A JP2001518457A JP4425513B2 JP 4425513 B2 JP4425513 B2 JP 4425513B2 JP 2001518457 A JP2001518457 A JP 2001518457A JP 2001518457 A JP2001518457 A JP 2001518457A JP 4425513 B2 JP4425513 B2 JP 4425513B2
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dna target
dna
medium
particles
target substance
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Expired - Lifetime
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JP2001518457A
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English (en)
Japanese (ja)
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JP2003507049A (ja
JP2003507049A5 (enExample
Inventor
アラン エム テリバ
レックス エム ビットナー
スーザン シー コラー
クレイグ イー スミス
ダニエル ディー ケファート
スティーブン ジェイ エイケンバーグ
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Promega Corp
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Promega Corp
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Priority claimed from US09/377,986 external-priority patent/US6673631B1/en
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Publication of JP2003507049A5 publication Critical patent/JP2003507049A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
JP2001518457A 1999-08-20 2000-08-18 Ｄｎａの同時単離及び定量化 Expired - Lifetime JP4425513B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/377,986 1999-08-20
US09/377,986 US6673631B1 (en) 1997-01-21 1999-08-20 Simultaneous isolation and quantitation of DNA
PCT/US2000/022827 WO2001014590A2 (en) 1999-08-20 2000-08-18 Simultaneous isolation and quantitation of dna

Publications (3)

Publication Number Publication Date
JP2003507049A JP2003507049A (ja) 2003-02-25
JP2003507049A5 JP2003507049A5 (enExample) 2005-11-17
JP4425513B2 true JP4425513B2 (ja) 2010-03-03

Family

ID=23491269

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001518457A Expired - Lifetime JP4425513B2 (ja) 1999-08-20 2000-08-18 Ｄｎａの同時単離及び定量化

Country Status (8)

Country Link
EP (1) EP1204741B1 (enExample)
JP (1) JP4425513B2 (enExample)
CN (1) CN1370230A (enExample)
AT (1) ATE280225T1 (enExample)
AU (1) AU780319B2 (enExample)
CA (1) CA2379503A1 (enExample)
DE (1) DE60015148T2 (enExample)
WO (1) WO2001014590A2 (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
ES2294287T5 (es) 2002-01-08 2015-10-27 F. Hoffmann-La Roche Ag Empleo de un material de sílice en una reacción de amplificación
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
EP1621890A1 (en) * 2004-07-26 2006-02-01 bioMerieux B.V. Device and method for separating, mixing and concentrating magnetic particles with a fluid and use thereof in purification methods
EP1629887B1 (de) * 2004-07-29 2007-04-11 Qiagen GmbH Monolithische Formkörper zur Aufreinigung und Trennung von Biopolymeren
EP1690938A1 (de) * 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
CN101467032B (zh) 2006-04-14 2013-03-27 日本电气株式会社 个体识别方法及设备
JP2009065900A (ja) * 2007-09-13 2009-04-02 Olympus Corp 標的ｒｎａの解析方法及び保存方法
US20110008776A1 (en) * 2007-11-26 2011-01-13 Atonomics A/S Integrated separation and detection cartridge using magnetic particles with bimodal size distribution
DE102008047790A1 (de) * 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
JP2013515956A (ja) * 2009-12-23 2013-05-09 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 検体測定装置及び方法
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
CN101818143A (zh) * 2010-04-28 2010-09-01 长春市博坤生物科技有限公司 基于二氧化硅磁性复合微粒的超低量dna提取试剂盒及方法
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
US9725754B2 (en) 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
WO2012013733A1 (en) 2010-07-29 2012-02-02 F. Hoffmann - La Roche Ag Generic sample preparation
US9777269B2 (en) * 2012-01-25 2017-10-03 Gencell Biosystems Ltd. Biomolecule isolation
AU2013240200B2 (en) * 2012-03-28 2016-10-20 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for the collection and isolation of nucleic acids from biological specimens
JP6170154B2 (ja) * 2012-08-28 2017-07-26 バイオキューブシステム インコーポレイテッド 生物学的試料から核酸増幅反応用生物学的分子を迅速に分離するための多孔性固体相及びその用途
CN105603056B (zh) * 2014-11-21 2020-09-25 丰益(上海)生物技术研发中心有限公司 一种植物油脂掺杂检测的方法
CN104560952A (zh) * 2014-12-29 2015-04-29 福建师范大学 一种基于磁珠法快速提取革兰氏阳性菌基因组dna的方法
WO2016183292A1 (en) 2015-05-14 2016-11-17 Longhorn Vaccines And Diagnostics, Llc Rapid methods for the extraction of nucleic acids from biological samples
EP3347490A1 (en) * 2015-09-11 2018-07-18 Corning Incorporated Compositions and methods for nucleic acid purification from blood samples
WO2018128585A1 (en) * 2017-01-04 2018-07-12 Agency For Science, Technology And Research Sieve-through vertical flow system for particle-based bioassays
CN109321634A (zh) * 2017-07-26 2019-02-12 上海之江生物科技股份有限公司 核酸均一化方法及其试剂盒和应用
WO2019035335A1 (ja) * 2017-08-18 2019-02-21 Ａｇｃ株式会社 核酸の回収方法、核酸結合用担体および核酸回収キット
US20230159912A1 (en) * 2021-11-23 2023-05-25 Bioo Scientific Corporation Compositions and methods for nucleic acid normalization

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles

Also Published As

Publication number Publication date
ATE280225T1 (de) 2004-11-15
JP2003507049A (ja) 2003-02-25
CA2379503A1 (en) 2001-03-01
CN1370230A (zh) 2002-09-18
AU780319B2 (en) 2005-03-17
EP1204741A2 (en) 2002-05-15
DE60015148T2 (de) 2006-02-16
WO2001014590A2 (en) 2001-03-01
AU6789500A (en) 2001-03-19
DE60015148D1 (de) 2004-11-25
EP1204741B1 (en) 2004-10-20
WO2001014590A3 (en) 2001-10-11

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