ATE280225T1 - Simultane isolierung und quantifizierung von dna - Google Patents
Simultane isolierung und quantifizierung von dnaInfo
- Publication number
- ATE280225T1 ATE280225T1 AT00955744T AT00955744T ATE280225T1 AT E280225 T1 ATE280225 T1 AT E280225T1 AT 00955744 T AT00955744 T AT 00955744T AT 00955744 T AT00955744 T AT 00955744T AT E280225 T1 ATE280225 T1 AT E280225T1
- Authority
- AT
- Austria
- Prior art keywords
- dna target
- target material
- methods
- dna
- complex
- Prior art date
Links
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000011002 quantification Methods 0.000 title 1
- 108020004414 DNA Proteins 0.000 abstract 8
- 239000013077 target material Substances 0.000 abstract 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 6
- 238000000034 method Methods 0.000 abstract 5
- 239000000377 silicon dioxide Substances 0.000 abstract 3
- 108091092878 Microsatellite Proteins 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 239000006249 magnetic particle Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 238000001712 DNA sequencing Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/377,986 US6673631B1 (en) | 1997-01-21 | 1999-08-20 | Simultaneous isolation and quantitation of DNA |
| PCT/US2000/022827 WO2001014590A2 (en) | 1999-08-20 | 2000-08-18 | Simultaneous isolation and quantitation of dna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE280225T1 true ATE280225T1 (de) | 2004-11-15 |
Family
ID=23491269
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00955744T ATE280225T1 (de) | 1999-08-20 | 2000-08-18 | Simultane isolierung und quantifizierung von dna |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1204741B1 (enExample) |
| JP (1) | JP4425513B2 (enExample) |
| CN (1) | CN1370230A (enExample) |
| AT (1) | ATE280225T1 (enExample) |
| AU (1) | AU780319B2 (enExample) |
| CA (1) | CA2379503A1 (enExample) |
| DE (1) | DE60015148T2 (enExample) |
| WO (1) | WO2001014590A2 (enExample) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60234464D1 (de) | 2001-11-28 | 2009-12-31 | Applied Biosystems Llc | Zusammensetzungen und Verfahren zur selektiven Nukleinsäureisolierung |
| ES2294287T5 (es) | 2002-01-08 | 2015-10-27 | F. Hoffmann-La Roche Ag | Empleo de un material de sílice en una reacción de amplificación |
| US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| EP1621890A1 (en) * | 2004-07-26 | 2006-02-01 | bioMerieux B.V. | Device and method for separating, mixing and concentrating magnetic particles with a fluid and use thereof in purification methods |
| EP1629887B1 (de) * | 2004-07-29 | 2007-04-11 | Qiagen GmbH | Monolithische Formkörper zur Aufreinigung und Trennung von Biopolymeren |
| EP1690938A1 (de) * | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| US20090277790A1 (en) | 2006-04-14 | 2009-11-12 | Nec Corporation | Individual identification method and apparatus |
| JP2009065900A (ja) * | 2007-09-13 | 2009-04-02 | Olympus Corp | 標的ｒｎａの解析方法及び保存方法 |
| WO2009068584A1 (en) * | 2007-11-26 | 2009-06-04 | Atonomics A/S | Integrated separation and detection cartridge with means and method for increasing signal to noise ratio |
| DE102008047790A1 (de) * | 2008-09-17 | 2010-04-15 | Qiagen Gmbh | Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe |
| WO2011077333A1 (en) * | 2009-12-23 | 2011-06-30 | Koninklijke Philips Electronics N.V. | Analyte measurement apparatus and method |
| US8420801B2 (en) * | 2010-01-08 | 2013-04-16 | Roche Molecular Systems, Inc. | Recovery of nucleic acids from magnetic glass particles |
| CN101818143A (zh) * | 2010-04-28 | 2010-09-01 | 长春市博坤生物科技有限公司 | 基于二氧化硅磁性复合微粒的超低量dna提取试剂盒及方法 |
| GB201010237D0 (en) | 2010-06-18 | 2010-07-21 | Lgc Ltd | Methods and apparatuses |
| US9725754B2 (en) | 2010-07-29 | 2017-08-08 | Sean F. Boyle | Generic sample preparation |
| JP6169489B2 (ja) | 2010-07-29 | 2017-07-26 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 一般的な試料調製 |
| BR112014018187A8 (pt) * | 2012-01-25 | 2017-07-11 | Gencell Biosystems Ltd | Métodos para manusear líquidos de amostra contendo partículas magnéticas e um líquido de encapsulação, bem como sistema de manuseio de líquido |
| AU2013240200B2 (en) * | 2012-03-28 | 2016-10-20 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for the collection and isolation of nucleic acids from biological specimens |
| WO2014035090A1 (ko) * | 2012-08-28 | 2014-03-06 | 주식회사 바이오큐브시스템 | 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도 |
| CN105603056B (zh) * | 2014-11-21 | 2020-09-25 | 丰益(上海)生物技术研发中心有限公司 | 一种植物油脂掺杂检测的方法 |
| CN104560952A (zh) * | 2014-12-29 | 2015-04-29 | 福建师范大学 | 一种基于磁珠法快速提取革兰氏阳性菌基因组dna的方法 |
| WO2016183292A1 (en) | 2015-05-14 | 2016-11-17 | Longhorn Vaccines And Diagnostics, Llc | Rapid methods for the extraction of nucleic acids from biological samples |
| JP2018526021A (ja) * | 2015-09-11 | 2018-09-13 | コーニング インコーポレイテッド | 血液試料からの核酸精製のための組成物及び方法 |
| US20190329244A1 (en) * | 2017-01-04 | 2019-10-31 | Agency For Science, Technology And Research | Sieve-through vertical flow system for particle-based bioassays |
| CN109321634A (zh) * | 2017-07-26 | 2019-02-12 | 上海之江生物科技股份有限公司 | 核酸均一化方法及其试剂盒和应用 |
| WO2019035335A1 (ja) * | 2017-08-18 | 2019-02-21 | Ａｇｃ株式会社 | 核酸の回収方法、核酸結合用担体および核酸回収キット |
| CN118284704A (zh) * | 2021-11-23 | 2024-07-02 | 柏尔科学公司 | 用于核酸标准化的组合物和方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
-
2000
- 2000-08-18 CN CN00811856.6A patent/CN1370230A/zh active Pending
- 2000-08-18 DE DE60015148T patent/DE60015148T2/de not_active Expired - Lifetime
- 2000-08-18 WO PCT/US2000/022827 patent/WO2001014590A2/en not_active Ceased
- 2000-08-18 AU AU67895/00A patent/AU780319B2/en not_active Ceased
- 2000-08-18 EP EP00955744A patent/EP1204741B1/en not_active Expired - Lifetime
- 2000-08-18 AT AT00955744T patent/ATE280225T1/de not_active IP Right Cessation
- 2000-08-18 CA CA002379503A patent/CA2379503A1/en not_active Abandoned
- 2000-08-18 JP JP2001518457A patent/JP4425513B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU780319B2 (en) | 2005-03-17 |
| WO2001014590A3 (en) | 2001-10-11 |
| AU6789500A (en) | 2001-03-19 |
| JP4425513B2 (ja) | 2010-03-03 |
| CA2379503A1 (en) | 2001-03-01 |
| CN1370230A (zh) | 2002-09-18 |
| JP2003507049A (ja) | 2003-02-25 |
| EP1204741A2 (en) | 2002-05-15 |
| EP1204741B1 (en) | 2004-10-20 |
| WO2001014590A2 (en) | 2001-03-01 |
| DE60015148T2 (de) | 2006-02-16 |
| DE60015148D1 (de) | 2004-11-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |