AU729932B2 - Methods for isolating nucleic acids using alkaline protease - Google Patents
Methods for isolating nucleic acids using alkaline protease Download PDFInfo
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- AU729932B2 AU729932B2 AU38214/97A AU3821497A AU729932B2 AU 729932 B2 AU729932 B2 AU 729932B2 AU 38214/97 A AU38214/97 A AU 38214/97A AU 3821497 A AU3821497 A AU 3821497A AU 729932 B2 AU729932 B2 AU 729932B2
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- solution
- nucleic acid
- alkaline
- dna
- protease
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Classifications
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- C—CHEMISTRY; METALLURGY
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/681922 | 1996-07-29 | ||
| US08/681,922 US5981235A (en) | 1996-07-29 | 1996-07-29 | Methods for isolating nucleic acids using alkaline protease |
| PCT/US1997/013440 WO1998004730A1 (en) | 1996-07-29 | 1997-07-29 | Methods for isolating nucleic acids using alkaline protease |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU3821497A AU3821497A (en) | 1998-02-20 |
| AU729932B2 true AU729932B2 (en) | 2001-02-15 |
Family
ID=24737412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU38214/97A Ceased AU729932B2 (en) | 1996-07-29 | 1997-07-29 | Methods for isolating nucleic acids using alkaline protease |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US5981235A (cg-RX-API-DMAC10.html) |
| EP (1) | EP0918877B1 (cg-RX-API-DMAC10.html) |
| JP (1) | JP3943601B2 (cg-RX-API-DMAC10.html) |
| AU (1) | AU729932B2 (cg-RX-API-DMAC10.html) |
| CA (1) | CA2260940C (cg-RX-API-DMAC10.html) |
| DE (1) | DE69730974T2 (cg-RX-API-DMAC10.html) |
| WO (1) | WO1998004730A1 (cg-RX-API-DMAC10.html) |
Families Citing this family (63)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0743949T3 (da) * | 1994-02-07 | 2000-04-10 | Qiagen Gmbh | Fremgangsmåde til fremstilling af endotoksinfrie eller endotoksinfattige nukleinsyrer og/eller oligonukleotider til gentera |
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6914137B2 (en) | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| ATE218140T1 (de) * | 1997-12-06 | 2002-06-15 | Dna Res Innovations Ltd | Isolierung von nukleinsäuren |
| US7078224B1 (en) * | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| US6194562B1 (en) * | 1998-04-22 | 2001-02-27 | Promega Corporation | Endotoxin reduction in nucleic acid purification |
| US6180408B1 (en) * | 1998-08-21 | 2001-01-30 | Washington University | Fluorescence polarization in nucleic acid analysis |
| EP1147226B1 (en) * | 1999-01-27 | 2013-01-23 | Folim G. Halaka | Materials and methods for the purification of polyelectrolytes |
| AU4367500A (en) * | 1999-04-21 | 2000-11-02 | Annovis, Inc. | Magnetic dna extraction kit for plants |
| US20020012990A1 (en) | 1999-12-22 | 2002-01-31 | Lander Russel Jackson | Process for the scaleable purification of plasmid DNA |
| US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| US7001724B1 (en) | 2000-11-28 | 2006-02-21 | Applera Corporation | Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases |
| DE10121463A1 (de) * | 2001-05-02 | 2003-02-27 | Henkel Kgaa | Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten |
| KR100454869B1 (ko) * | 2001-07-13 | 2004-11-03 | 주식회사 인트론바이오테크놀로지 | Ｄｎａ 추출용 세포 용해 버퍼 및 이를 이용한 ｄｎａ 추출방법 |
| DE10153957A1 (de) * | 2001-11-06 | 2003-05-22 | Quiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| EP1448799B2 (en) | 2001-11-28 | 2018-05-16 | Life Technologies Corporation | Methods of selective nucleic acid isolation |
| CA2473376A1 (en) | 2002-01-16 | 2003-07-31 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| US7364846B2 (en) * | 2002-10-11 | 2008-04-29 | Molecular Devices Corporation | Gene expression profiling from FFPE samples |
| US7319021B2 (en) * | 2002-11-01 | 2008-01-15 | Promega Corporation | Cell lysis composition, methods of use, apparatus and kit |
| US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
| JP2006526591A (ja) * | 2003-06-04 | 2006-11-24 | キアゲン アクティーゼルスカブ | 核酸または核酸含有種の濃縮及び／または単離方法 |
| US20050164204A1 (en) * | 2004-01-27 | 2005-07-28 | Reed Thomas D. | Single use lyophilized rnase reagents, and kits and methods for using same |
| AU2005200670B2 (en) * | 2004-02-20 | 2007-05-03 | F. Hoffmann-La Roche Ag | Adsorption of nucleic acids to a solid phase |
| KR100486179B1 (ko) * | 2004-03-02 | 2005-05-03 | 피경태 | 핵산을 분리하고 정제하기 위한 세포 용해 조성물, 방법 및 키트 |
| US7794932B2 (en) * | 2004-04-16 | 2010-09-14 | Piotr Chomczynski | Reagents and methods for isolation of purified RNA |
| CN101310022B (zh) | 2005-01-31 | 2013-01-16 | 默沙东公司 | 大规模生产质粒dna的上游和下游纯化方法 |
| US20060188892A1 (en) * | 2005-02-18 | 2006-08-24 | Ambion, Inc. | Enzymatic digestion of tissue |
| JP2009500019A (ja) * | 2005-07-01 | 2009-01-08 | プロメガ・コーポレーション | 生体分子の精製のための浮遊性粒子のネットワーク、及び生体分子の精製のための浮遊性粒子又は浮遊性粒子のネットワークの使用 |
| EP1749892B1 (en) | 2005-08-02 | 2008-03-19 | Roche Diagnostics GmbH | Nucleotide sequence for assessing TSE |
| US7569367B2 (en) | 2005-09-07 | 2009-08-04 | Roche Diagnostics Operations, Inc. | Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy |
| WO2007070381A2 (en) | 2005-12-09 | 2007-06-21 | Promega Corporation | Nucleic acid purification with a binding matrix |
| JP5354894B2 (ja) | 2006-12-11 | 2013-11-27 | エフ．ホフマン−ラ ロシュ アーゲー | ポリドカノールおよび誘導体を用いた核酸単離 |
| EP1932913B1 (en) | 2006-12-11 | 2013-01-16 | Roche Diagnostics GmbH | Nucleic acid isolation using polidocanol and derivatives |
| US8535888B2 (en) | 2006-12-29 | 2013-09-17 | Mayo Foundation For Medical Education And Research | Compositions and methods for detecting methicillin-resistant S. aureus |
| DK2181191T3 (da) * | 2007-08-20 | 2011-05-23 | Novozymes As | Nukleasereduktion |
| US8007205B2 (en) * | 2008-04-09 | 2011-08-30 | Marshall Iii Henry Bennie | Foundation wall vapor barrier system |
| EP2130929B1 (en) | 2008-06-06 | 2013-10-09 | F. Hoffmann-La Roche AG | Internally controlled multiplex detection and quantification of microbial nucleic acids |
| CN101619355B (zh) * | 2009-07-02 | 2012-02-08 | 黑龙江大学 | 传统发酵豆酱中细菌的检测方法 |
| US8222397B2 (en) | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| WO2011026194A1 (en) * | 2009-09-07 | 2011-03-10 | Reproductive Health Science Pty Ltd | Nucleic acid extraction |
| CN107058617A (zh) | 2010-07-29 | 2017-08-18 | 霍夫曼-拉罗奇有限公司 | 通用样品制备 |
| CA2802567C (en) | 2010-07-29 | 2017-12-05 | F. Hoffmann La-Roche Ag | Control nucleic acids for multiple parameters |
| CN103270171B (zh) | 2010-07-29 | 2014-09-17 | 霍夫曼-拉罗奇有限公司 | 通用pcr |
| JP5992909B2 (ja) | 2010-07-29 | 2016-09-14 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 微生物核酸の定性的および定量的検出 |
| EP2426222A1 (en) | 2010-09-07 | 2012-03-07 | F. Hoffmann-La Roche AG | Generic buffer for amplification |
| EP2465945A1 (en) | 2010-12-17 | 2012-06-20 | F. Hoffmann-La Roche AG | Generic matrix for control nucleic acids |
| WO2013067069A2 (en) * | 2011-10-31 | 2013-05-10 | Leinweber Barbara | Dual digestion method for high yield nucleic acid recovery |
| US9057673B2 (en) | 2012-08-24 | 2015-06-16 | The Board Of Trustees Of The Leland Stanford Junior University | Method of preparing RNA from ribonuclease-rich sources |
| US20160010081A1 (en) * | 2014-07-11 | 2016-01-14 | Exact Sciences Corporation | Systems, methods and kits for extracting nucleic acid from digestive fluid |
| WO2016057891A1 (en) | 2014-10-09 | 2016-04-14 | Promega Corporation | Method of isolating nucleic acid |
| CN107257857B (zh) | 2014-11-14 | 2021-12-14 | 康宁股份有限公司 | 用于ivt后rna纯化的方法和试剂盒 |
| CN107208089A (zh) * | 2014-12-09 | 2017-09-26 | 康宁股份有限公司 | 用于纯化质粒dna的方法和试剂盒 |
| ES2759477T3 (es) | 2015-05-29 | 2020-05-11 | Hoffmann La Roche | Un procedimiento de inactivación de microbios por anhídrido citracónico |
| SG11201806190TA (en) | 2016-01-29 | 2018-08-30 | Purigen Biosystems Inc | Isotachophoresis for purification of nucleic acids |
| AU2018312570B2 (en) | 2017-08-02 | 2024-01-11 | Purigen Biosystems, Inc. | Systems, devices, and methods for isotachophoresis |
| GB201803654D0 (en) * | 2018-03-07 | 2018-04-25 | Arcticzymes As | Thermolabile proteinases |
| KR20230139598A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
| KR20230139597A (ko) | 2022-03-28 | 2023-10-05 | 씨제이제일제당 (주) | O-아세틸 호모세린 생산 미생물 및 이를 이용한 o-아세틸 호모세린 또는 l-메치오닌 생산 방법 |
| KR20240152456A (ko) | 2023-04-12 | 2024-10-22 | 씨제이제일제당 (주) | 피루브산 탈수소효소 복합체 서브유닛 e1의 활성이 증가된 미생물 및 이를 이용한 o-아세틸호모세린 또는 그의 유도체의 생산 방법 |
| KR20240152455A (ko) | 2023-04-12 | 2024-10-22 | 씨제이제일제당 (주) | 피루브산 탈수소효소 복합체 디히드로리포일리신-잔기 아세틸트랜스퍼라제의 활성이 증가된 미생물 및 이를 이용한 l-아미노산 생산 방법 |
| GB202319423D0 (en) * | 2023-12-18 | 2024-01-31 | BioNTech SE | Measuring and reducing protein impurities |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
| US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| WO1995006652A1 (en) * | 1993-08-30 | 1995-03-09 | Promega Corporation | Nucleic acid purification compositions and methods |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4843155A (en) * | 1987-11-19 | 1989-06-27 | Piotr Chomczynski | Product and process for isolating RNA |
| US5075430A (en) * | 1988-12-12 | 1991-12-24 | Bio-Rad Laboratories, Inc. | Process for the purification of DNA on diatomaceous earth |
| US5155018A (en) * | 1991-07-10 | 1992-10-13 | Hahnemann University | Process and kit for isolating and purifying RNA from biological sources |
| US5256557A (en) * | 1991-12-27 | 1993-10-26 | Solvay Enzymes, Inc. | Purified alkaline protease concentrate and method of preparation |
| EP0708828B1 (en) * | 1993-07-08 | 1997-03-12 | Tepnel Medical Limited | Obtaining nucleic acids |
| US5646263A (en) * | 1994-09-19 | 1997-07-08 | Promega Corporation | High efficiency method for isolating target substances using a multisample separation device |
-
1996
- 1996-07-29 US US08/681,922 patent/US5981235A/en not_active Expired - Lifetime
-
1997
- 1997-07-29 CA CA002260940A patent/CA2260940C/en not_active Expired - Fee Related
- 1997-07-29 JP JP50913998A patent/JP3943601B2/ja not_active Expired - Fee Related
- 1997-07-29 DE DE69730974T patent/DE69730974T2/de not_active Expired - Lifetime
- 1997-07-29 WO PCT/US1997/013440 patent/WO1998004730A1/en not_active Ceased
- 1997-07-29 EP EP97935224A patent/EP0918877B1/en not_active Expired - Lifetime
- 1997-07-29 AU AU38214/97A patent/AU729932B2/en not_active Ceased
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4900677A (en) * | 1986-09-26 | 1990-02-13 | E. I. Du Pont De Nemours And Company | Process for rapid isolation of high molecular weight DNA |
| US5386024A (en) * | 1993-02-10 | 1995-01-31 | Gen-Probe Incorporated | Method to prepare nucleic acids from a biological sample using low pH and acid protease |
| WO1995006652A1 (en) * | 1993-08-30 | 1995-03-09 | Promega Corporation | Nucleic acid purification compositions and methods |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0918877A4 (en) | 2001-01-31 |
| JP2000516091A (ja) | 2000-12-05 |
| CA2260940A1 (en) | 1998-02-05 |
| EP0918877B1 (en) | 2004-09-29 |
| CA2260940C (en) | 2007-05-15 |
| AU3821497A (en) | 1998-02-20 |
| WO1998004730A1 (en) | 1998-02-05 |
| DE69730974D1 (de) | 2004-11-04 |
| EP0918877A1 (en) | 1999-06-02 |
| DE69730974T2 (de) | 2006-02-23 |
| JP3943601B2 (ja) | 2007-07-11 |
| US5981235A (en) | 1999-11-09 |
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| FGA | Letters patent sealed or granted (standard patent) |