AU725966B2 - Mass label linked hybridisation probes - Google Patents
Mass label linked hybridisation probes Download PDFInfo
- Publication number
- AU725966B2 AU725966B2 AU55700/98A AU5570098A AU725966B2 AU 725966 B2 AU725966 B2 AU 725966B2 AU 55700/98 A AU55700/98 A AU 55700/98A AU 5570098 A AU5570098 A AU 5570098A AU 725966 B2 AU725966 B2 AU 725966B2
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- AU
- Australia
- Prior art keywords
- mass
- probe
- sequence
- base sequence
- hybridisation
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6872—Methods for sequencing involving mass spectrometry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Discharge Heating (AREA)
- Adhesives Or Adhesive Processes (AREA)
- Surgical Instruments (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Ultra Sonic Daignosis Equipment (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9700746 | 1997-01-15 | ||
| GBGB9700746.2A GB9700746D0 (en) | 1996-10-04 | 1997-01-15 | Hybridisation probes |
| GBGB9718255.4A GB9718255D0 (en) | 1996-10-04 | 1997-08-28 | Hybridisation probes |
| GB9718255 | 1997-08-28 | ||
| GBGB9726953.4A GB9726953D0 (en) | 1997-12-19 | 1997-12-19 | Hybridisation probes |
| GB9726953 | 1997-12-19 | ||
| PCT/GB1998/000127 WO1998031830A1 (en) | 1997-01-15 | 1998-01-15 | Mass label linked hybridisation probes |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU5570098A AU5570098A (en) | 1998-08-07 |
| AU725966B2 true AU725966B2 (en) | 2000-10-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU55700/98A Ceased AU725966B2 (en) | 1997-01-15 | 1998-01-15 | Mass label linked hybridisation probes |
Country Status (10)
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| EP (2) | EP1400599B1 (enExample) |
| JP (3) | JP3884087B2 (enExample) |
| CN (1) | CN100434531C (enExample) |
| AT (2) | ATE374836T1 (enExample) |
| AU (1) | AU725966B2 (enExample) |
| CA (2) | CA2441655A1 (enExample) |
| DE (2) | DE69838519T2 (enExample) |
| IL (1) | IL130885A (enExample) |
| NZ (1) | NZ336769A (enExample) |
| WO (1) | WO1998031830A1 (enExample) |
Families Citing this family (94)
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| GB9707980D0 (en) | 1997-04-21 | 1997-06-11 | Brax Genomics Ltd | Characterising DNA |
| US6312904B1 (en) * | 1997-07-11 | 2001-11-06 | Xzillion Gmbh & Co. Kg | Characterizing nucleic acid |
| ATE240408T1 (de) * | 1997-07-22 | 2003-05-15 | Qiagen Genomics Inc | Verfahren und verbindungen zur analysierung von nukleinsaeuren durch massenspektrometrie |
| GB9718921D0 (en) * | 1997-09-05 | 1997-11-12 | Brax Genomics Ltd | Catalytically generated mass labels |
| WO1999014362A1 (en) * | 1997-09-15 | 1999-03-25 | Brax Group Limited | Characterising nucleic acid by mass spectrometry |
| ATE217028T1 (de) * | 1997-12-05 | 2002-05-15 | Max Planck Gesellschaft | Verfahren zur identifikation von nucleinsäuren durch matrix-assistierte laser desorptions/ionisations massenspektrometrie |
| GB9815163D0 (en) * | 1998-07-13 | 1998-09-09 | Brax Genomics Ltd | Compounds |
| GB9823646D0 (en) * | 1997-12-19 | 1998-12-23 | Brax Genomics Ltd | Compounds for mass spectrometry |
| GB9815164D0 (en) * | 1998-07-13 | 1998-09-09 | Brax Genomics Ltd | Compounds for mass spectrometry |
| GB9815166D0 (en) * | 1998-07-13 | 1998-09-09 | Brax Genomics Ltd | Compounds for mass spectrometry |
| US6780981B1 (en) | 1998-05-15 | 2004-08-24 | Oxford Gene Technology Ip Limited | Libraries of oligomers labeled with different tags |
| US7399844B2 (en) * | 1998-07-09 | 2008-07-15 | Agilent Technologies, Inc. | Method and reagents for analyzing the nucleotide sequence of nucleic acids |
| US6270976B1 (en) | 1998-09-15 | 2001-08-07 | Brax Group Limited | Characterizing nucleic acid by mass spectrometry |
| JP3668075B2 (ja) * | 1999-10-12 | 2005-07-06 | 光夫 板倉 | 遺伝物質シーケンス決定用懸濁系、その懸濁系を用いた遺伝物質シーケンス決定方法およびその懸濁系を用いたＳＮＰｓ高速スコアリング方法 |
| US6509157B1 (en) * | 1999-11-05 | 2003-01-21 | Roche Molecular Systems, Inc | 3 blocked nucleic acid amplification primers |
| DE19963536C2 (de) * | 1999-12-20 | 2003-04-10 | Epigenomics Ag | Verfahren zur Analyse von Nukleinsäuresequenzen |
| JP2001204463A (ja) * | 2000-01-27 | 2001-07-31 | Toyo Kohan Co Ltd | ヌクレオチド固定用担体 |
| GB0006141D0 (en) | 2000-03-14 | 2000-05-03 | Brax Group Ltd | Mass labels |
| EP1283909A4 (en) | 2000-05-04 | 2006-06-07 | Univ Yale | HIGH DENSITY PROTEIN ARRAYS FOR SCREENING PROTEIN ACTIVITY |
| CA2419159A1 (en) | 2000-08-11 | 2002-02-21 | Agilix Corporation | Ultra-sensitive detection systems |
| ES2385335T3 (es) | 2000-10-19 | 2012-07-23 | Target Discovery, Inc. | Marcaje de defecto de masa para la determinación de secuencias de oligómeros |
| WO2002050307A1 (en) * | 2000-12-12 | 2002-06-27 | Chugai Seiyaku Kabushiki Kaisha | Method of detecting polymorphism in dna by using mass spectroscopy |
| US20040121313A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in organs for transplantation |
| US20030027135A1 (en) | 2001-03-02 | 2003-02-06 | Ecker David J. | Method for rapid detection and identification of bioagents |
| US7226739B2 (en) | 2001-03-02 | 2007-06-05 | Isis Pharmaceuticals, Inc | Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations |
| US7666588B2 (en) | 2001-03-02 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
| US7217510B2 (en) | 2001-06-26 | 2007-05-15 | Isis Pharmaceuticals, Inc. | Methods for providing bacterial bioagent characterizing information |
| DK1425586T3 (da) | 2001-09-14 | 2008-02-11 | Electrophoretics Ltd | Massemarkörer |
| JP4606028B2 (ja) * | 2002-04-04 | 2011-01-05 | エレクトロフォレティクス リミテッド | 被分析物の分析方法 |
| JP2004037128A (ja) * | 2002-06-28 | 2004-02-05 | Canon Inc | マトリクス援助レーザー脱着／イオン化飛行時間型質量分析法による基板上の物質の解析方法 |
| AU2003304026B2 (en) | 2002-10-29 | 2010-03-25 | Target Discovery, Inc. | Method for increasing ionization efficiency in mass spectroscopy |
| JP2006516193A (ja) | 2002-12-06 | 2006-06-29 | アイシス・ファーマシューティカルス・インコーポレーテッド | ヒトおよび動物における病原体の迅速な同定方法 |
| US7799576B2 (en) | 2003-01-30 | 2010-09-21 | Dh Technologies Development Pte. Ltd. | Isobaric labels for mass spectrometric analysis of peptides and method thereof |
| US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
| US8394945B2 (en) | 2003-09-11 | 2013-03-12 | Ibis Biosciences, Inc. | Compositions for use in identification of bacteria |
| US8546082B2 (en) | 2003-09-11 | 2013-10-01 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| US8097416B2 (en) | 2003-09-11 | 2012-01-17 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
| JP2005140755A (ja) * | 2003-11-10 | 2005-06-02 | Japan Science & Technology Agency | 質量分析用プローブ及びそれを用いた質量分析方法 |
| US20050148087A1 (en) | 2004-01-05 | 2005-07-07 | Applera Corporation | Isobarically labeled analytes and fragment ions derived therefrom |
| US20050148771A1 (en) | 2004-01-05 | 2005-07-07 | Applera Corporation. | Active esters of N-substituted piperazine acetic acids, including isotopically enriched versions thereof |
| US7355045B2 (en) | 2004-01-05 | 2008-04-08 | Applera Corporation | Isotopically enriched N-substituted piperazine acetic acids and methods for the preparation thereof |
| US7666592B2 (en) | 2004-02-18 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
| DE602005001302T2 (de) | 2004-03-01 | 2008-02-14 | Applera Corp., Framingham | Bestimmung von analytcharakteristika auf der grundlage von bindungseigenschaften |
| ES2641832T3 (es) | 2004-05-24 | 2017-11-14 | Ibis Biosciences, Inc. | Espectrometría de masas con filtración de iones selectiva por establecimiento de umbrales digitales |
| US20050266411A1 (en) | 2004-05-25 | 2005-12-01 | Hofstadler Steven A | Methods for rapid forensic analysis of mitochondrial DNA |
| US20070048752A1 (en) | 2004-07-12 | 2007-03-01 | Applera Corporation | Mass tags for quantitative analyses |
| US7811753B2 (en) | 2004-07-14 | 2010-10-12 | Ibis Biosciences, Inc. | Methods for repairing degraded DNA |
| AU2005280528B2 (en) | 2004-07-30 | 2010-12-23 | Adeza Biomedical Corporation | Oncofetal fibronectin as a marker for disease and other conditions and methods for detection of oncofetal fibronectin |
| WO2006135400A2 (en) | 2004-08-24 | 2006-12-21 | Isis Pharmaceuticals, Inc. | Methods for rapid identification of recombinant organisms |
| US20060183238A1 (en) | 2005-02-09 | 2006-08-17 | Applera Corporation | Amine-containing compound analysis methods |
| US20060205040A1 (en) | 2005-03-03 | 2006-09-14 | Rangarajan Sampath | Compositions for use in identification of adventitious viruses |
| US8084207B2 (en) | 2005-03-03 | 2011-12-27 | Ibis Bioscience, Inc. | Compositions for use in identification of papillomavirus |
| WO2007014045A2 (en) | 2005-07-21 | 2007-02-01 | Isis Pharmaceuticals, Inc. | Methods for rapid identification and quantitation of nucleic acid variants |
| GB0515323D0 (en) | 2005-07-26 | 2005-08-31 | Electrophoretics Ltd | Mass labels |
| GB0518585D0 (en) | 2005-09-12 | 2005-10-19 | Electrophoretics Ltd | Mass labels |
| US8975404B2 (en) | 2006-01-24 | 2015-03-10 | Dh Technologies Development Pte. Ltd. | Labeling reagents for analyte determination and methods and compounds used in making the same |
| US8088582B2 (en) | 2006-04-06 | 2012-01-03 | Ibis Biosciences, Inc. | Compositions for the use in identification of fungi |
| EP2054540B1 (en) | 2006-05-26 | 2014-11-26 | DH Technologies Development Pte. Ltd. | Tagging reagents and methods for hydroxylated compounds |
| US11098345B2 (en) | 2006-06-05 | 2021-08-24 | California Institute Of Technology | Methods for detecting target analytes |
| US8362242B2 (en) | 2006-06-30 | 2013-01-29 | Dh Technologies Development Pte. Ltd. | Analyte determination utilizing mass tagging reagents comprising a non-encoded detectable label |
| US7906341B2 (en) | 2006-06-30 | 2011-03-15 | Dh Technologies Development Pte, Ltd. | Methods, mixtures, kits and compositions pertaining to analyte determination |
| US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| EP2064332B1 (en) | 2006-09-14 | 2012-07-18 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
| US8871471B2 (en) | 2007-02-23 | 2014-10-28 | Ibis Biosciences, Inc. | Methods for rapid forensic DNA analysis |
| GB0704764D0 (en) | 2007-03-12 | 2007-04-18 | Electrophoretics Ltd | Isobarically labelled reagents and methods of their use |
| US9598724B2 (en) | 2007-06-01 | 2017-03-21 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
| CN101519698B (zh) * | 2008-03-10 | 2013-05-22 | 周国华 | 一种利用序列标签定量测定核酸的方法 |
| US8148163B2 (en) | 2008-09-16 | 2012-04-03 | Ibis Biosciences, Inc. | Sample processing units, systems, and related methods |
| WO2010033599A2 (en) | 2008-09-16 | 2010-03-25 | Ibis Biosciences, Inc. | Mixing cartridges, mixing stations, and related kits, systems, and methods |
| EP2344893B1 (en) | 2008-09-16 | 2014-10-15 | Ibis Biosciences, Inc. | Microplate handling systems and methods |
| US8158936B2 (en) | 2009-02-12 | 2012-04-17 | Ibis Biosciences, Inc. | Ionization probe assemblies |
| US9719083B2 (en) | 2009-03-08 | 2017-08-01 | Ibis Biosciences, Inc. | Bioagent detection methods |
| US8486712B2 (en) | 2009-03-10 | 2013-07-16 | University Of Maryland, College Park | Deuterium isobaric tag reagents for quantitative analysis |
| WO2010114842A1 (en) | 2009-03-30 | 2010-10-07 | Ibis Biosciences, Inc. | Bioagent detection systems, devices, and methods |
| WO2011008972A1 (en) | 2009-07-17 | 2011-01-20 | Ibis Biosciences, Inc. | Systems for bioagent identification |
| WO2011008971A1 (en) | 2009-07-17 | 2011-01-20 | Ibis Biosciences, Inc. | Lift and mount apparatus |
| US9416409B2 (en) | 2009-07-31 | 2016-08-16 | Ibis Biosciences, Inc. | Capture primers and capture sequence linked solid supports for molecular diagnostic tests |
| EP3098325A1 (en) | 2009-08-06 | 2016-11-30 | Ibis Biosciences, Inc. | Non-mass determined base compositions for nucleic acid detection |
| US9890408B2 (en) | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
| WO2011115840A2 (en) | 2010-03-14 | 2011-09-22 | Ibis Biosciences, Inc. | Parasite detection via endosymbiont detection |
| EP2588144B1 (en) | 2010-07-02 | 2018-05-09 | Ventana Medical Systems, Inc. | Detecting targets using mass tags and mass spectrometry |
| US8492163B2 (en) | 2011-01-31 | 2013-07-23 | Dh Technologies Development Pte. Ltd. | Methods, mixtures, kits and compositions pertaining to analyte determination |
| US9708647B2 (en) * | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
| US20170089915A1 (en) * | 2015-09-30 | 2017-03-30 | Agilent Technologies, Inc. | Methods of analyte derivatization and enhanced soft ionization |
| JP2019503174A (ja) * | 2016-01-04 | 2019-02-07 | クァンタムディーエックス グループ リミテッドＱｕａｎｔｕｍｄｘ Ｇｒｏｕｐ Ｌｉｍｉｔｅｄ | 電荷量タグを含む合成ヌクレオチドのデザイン、合成、および使用 |
| WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
| CN119510370A (zh) | 2019-03-14 | 2025-02-25 | 因斯利克萨公司 | 基于时间门控的荧光检测的方法和系统 |
| EP4240872A1 (en) * | 2020-11-06 | 2023-09-13 | Waters Technologies Corporation | Methods and compositions useful for nucleic acid analysis |
| WO2023282061A1 (ja) * | 2021-07-08 | 2023-01-12 | 株式会社島津製作所 | 微生物の質量分析方法 |
| CN116496335B (zh) * | 2023-03-21 | 2024-03-08 | 中国药科大学 | 一种修饰核苷酸及其应用 |
| CN118980740B (zh) * | 2024-09-19 | 2025-01-24 | 常州越泰检测科技有限公司 | 一种磁粉检测用喷照一体装置 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995004160A1 (en) * | 1993-07-30 | 1995-02-09 | Isis Innovation Limited | Tag reagent and assay method |
| EP0735144A1 (en) * | 1995-03-28 | 1996-10-02 | Research Development Corporation Of Japan | Method for molecular indexing categorising of expressed genes using restriction enzymes |
| WO1996032504A2 (en) * | 1995-04-11 | 1996-10-17 | Trustees Of Boston University | Solid phase sequencing of biopolymers |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69433811T2 (de) * | 1993-01-07 | 2005-06-23 | Sequenom, Inc., San Diego | Dns - sequenzierung durch massenspektronomie |
| CZ228598A3 (cs) * | 1996-01-23 | 1998-12-16 | Rapigene, Inc. | Způsoby a kompozice pro analyzování molekul nukleových kyselin za použití postupu třídění |
| GB9618544D0 (en) * | 1996-09-05 | 1996-10-16 | Brax Genomics Ltd | Characterising DNA |
-
1998
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- 1998-01-15 JP JP53394298A patent/JP3884087B2/ja not_active Expired - Fee Related
- 1998-01-15 CN CNB988032872A patent/CN100434531C/zh not_active Expired - Fee Related
- 1998-01-15 NZ NZ336769A patent/NZ336769A/xx unknown
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- 1998-01-15 AT AT03076965T patent/ATE374836T1/de not_active IP Right Cessation
- 1998-01-15 AU AU55700/98A patent/AU725966B2/en not_active Ceased
- 1998-01-15 AT AT98900611T patent/ATE340869T1/de not_active IP Right Cessation
- 1998-01-15 CA CA002441655A patent/CA2441655A1/en not_active Abandoned
- 1998-01-15 CA CA002277786A patent/CA2277786A1/en active Pending
- 1998-01-15 DE DE69836013T patent/DE69836013T2/de not_active Expired - Lifetime
- 1998-01-15 EP EP03076965A patent/EP1400599B1/en not_active Expired - Lifetime
- 1998-01-15 IL IL13088598A patent/IL130885A/xx not_active IP Right Cessation
- 1998-01-15 EP EP98900611A patent/EP0979305B1/en not_active Expired - Lifetime
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- 2004-02-13 JP JP2004036206A patent/JP2004357697A/ja not_active Withdrawn
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- 2006-04-19 JP JP2006115777A patent/JP2006208400A/ja active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995004160A1 (en) * | 1993-07-30 | 1995-02-09 | Isis Innovation Limited | Tag reagent and assay method |
| EP0735144A1 (en) * | 1995-03-28 | 1996-10-02 | Research Development Corporation Of Japan | Method for molecular indexing categorising of expressed genes using restriction enzymes |
| WO1996032504A2 (en) * | 1995-04-11 | 1996-10-17 | Trustees Of Boston University | Solid phase sequencing of biopolymers |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001508307A (ja) | 2001-06-26 |
| DE69836013T2 (de) | 2007-05-24 |
| JP2006208400A (ja) | 2006-08-10 |
| IL130885A0 (en) | 2001-01-28 |
| EP0979305A1 (en) | 2000-02-16 |
| DE69838519D1 (de) | 2007-11-15 |
| EP1400599B1 (en) | 2007-10-03 |
| NZ336769A (en) | 2001-01-26 |
| CA2441655A1 (en) | 1998-07-23 |
| AU5570098A (en) | 1998-08-07 |
| EP0979305B1 (en) | 2006-09-27 |
| JP3884087B2 (ja) | 2007-02-21 |
| DE69836013D1 (de) | 2006-11-09 |
| WO1998031830A1 (en) | 1998-07-23 |
| CA2277786A1 (en) | 1998-07-23 |
| IL130885A (en) | 2005-05-17 |
| ATE340869T1 (de) | 2006-10-15 |
| CN100434531C (zh) | 2008-11-19 |
| JP2004357697A (ja) | 2004-12-24 |
| DE69838519T2 (de) | 2008-07-03 |
| CN1250483A (zh) | 2000-04-12 |
| ATE374836T1 (de) | 2007-10-15 |
| EP1400599A1 (en) | 2004-03-24 |
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