AU2021224852A1 - Methods of preparing oligonucleotide compositions using ultrafiltration / diafiltration - Google Patents
Methods of preparing oligonucleotide compositions using ultrafiltration / diafiltration Download PDFInfo
- Publication number
- AU2021224852A1 AU2021224852A1 AU2021224852A AU2021224852A AU2021224852A1 AU 2021224852 A1 AU2021224852 A1 AU 2021224852A1 AU 2021224852 A AU2021224852 A AU 2021224852A AU 2021224852 A AU2021224852 A AU 2021224852A AU 2021224852 A1 AU2021224852 A1 AU 2021224852A1
- Authority
- AU
- Australia
- Prior art keywords
- acetate
- oligonucleotide
- sodium
- buffer solution
- retentate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/313—Phosphorodithioates
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicinal Preparation (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202062979687P | 2020-02-21 | 2020-02-21 | |
US62/979,687 | 2020-02-21 | ||
PCT/US2021/018856 WO2021168306A1 (en) | 2020-02-21 | 2021-02-19 | Methods of preparing oligonucleotide compositions using ultrafiltration / diafiltration |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2021224852A1 true AU2021224852A1 (en) | 2022-10-13 |
Family
ID=74870921
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2021224852A Pending AU2021224852A1 (en) | 2020-02-21 | 2021-02-19 | Methods of preparing oligonucleotide compositions using ultrafiltration / diafiltration |
Country Status (11)
Country | Link |
---|---|
US (1) | US20230116671A1 (ja) |
EP (1) | EP4107261A1 (ja) |
JP (1) | JP2023515485A (ja) |
KR (1) | KR20220156843A (ja) |
CN (1) | CN115997013A (ja) |
AU (1) | AU2021224852A1 (ja) |
BR (1) | BR112022016598A2 (ja) |
CA (1) | CA3168716A1 (ja) |
IL (1) | IL295757A (ja) |
MX (1) | MX2022010310A (ja) |
WO (1) | WO2021168306A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4329923A1 (en) * | 2021-04-30 | 2024-03-06 | Hydranautics | Concentration and diafiltration of oligonucleotides |
WO2023164631A1 (en) * | 2022-02-25 | 2023-08-31 | Amgen Inc. | Methods of preparing high concentration liquid drug substances |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7074333B2 (en) * | 2001-01-19 | 2006-07-11 | Millipore Corporation | Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration |
GB201012418D0 (en) * | 2010-07-23 | 2010-09-08 | Santaris Pharma As | Process |
BR112015022660A2 (pt) * | 2013-03-14 | 2017-10-31 | Shire Human Genetic Therapies | métodos para a purificação de rna mensageiro |
ES2923018T3 (es) * | 2013-03-15 | 2022-09-22 | Glaxosmithkline Biologicals Sa | Métodos de purificación de ARN |
CN107873055B (zh) * | 2015-05-29 | 2021-09-17 | 库瑞瓦格房地产有限公司 | 包括至少一个切向流过滤步骤的产生和纯化rna的方法 |
EP3658155A4 (en) * | 2017-07-28 | 2021-06-30 | Nogra Pharma Limited | PROCESS FOR THE PREPARATION OF OLIGONUCLEOTIDIC COMPOUNDS |
-
2021
- 2021-02-19 IL IL295757A patent/IL295757A/en unknown
- 2021-02-19 WO PCT/US2021/018856 patent/WO2021168306A1/en unknown
- 2021-02-19 US US17/801,196 patent/US20230116671A1/en active Pending
- 2021-02-19 JP JP2022549837A patent/JP2023515485A/ja active Pending
- 2021-02-19 BR BR112022016598A patent/BR112022016598A2/pt unknown
- 2021-02-19 MX MX2022010310A patent/MX2022010310A/es unknown
- 2021-02-19 AU AU2021224852A patent/AU2021224852A1/en active Pending
- 2021-02-19 EP EP21711447.9A patent/EP4107261A1/en active Pending
- 2021-02-19 KR KR1020227032773A patent/KR20220156843A/ko unknown
- 2021-02-19 CA CA3168716A patent/CA3168716A1/en active Pending
- 2021-02-19 CN CN202180030178.9A patent/CN115997013A/zh active Pending
Also Published As
Publication number | Publication date |
---|---|
EP4107261A1 (en) | 2022-12-28 |
MX2022010310A (es) | 2023-01-24 |
WO2021168306A1 (en) | 2021-08-26 |
BR112022016598A2 (pt) | 2022-11-08 |
CA3168716A1 (en) | 2021-08-26 |
CN115997013A (zh) | 2023-04-21 |
IL295757A (en) | 2022-10-01 |
US20230116671A1 (en) | 2023-04-13 |
KR20220156843A (ko) | 2022-11-28 |
JP2023515485A (ja) | 2023-04-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20230116671A1 (en) | Methods of preparing oligonucleotide compositions using ultrafiltration/diafiltration | |
DE60128945T2 (de) | Verfahren zur abtrennung von extrachromosomaler dns aus einer zellsuspension | |
BRPI0809212A2 (pt) | Processo de purificação abreviado para a produção de polissacarídeos capsulares de streptococcus pneumoniae | |
EP2723760B1 (de) | Verfahren zur abtrennung von biopolymer-aggregaten und viren aus einem fluid | |
AU2017313268B2 (en) | Process for the preparation of highly concentrated liquid formulations containing biomolecules | |
JP2022521161A (ja) | タンジェントフローろ過によるmRNA精製 | |
EP3185887B1 (en) | Method for preparing crystalline insulin or insulin analog compositions | |
EP3077414B1 (en) | Method for preparing crystalline insulin | |
US20230174966A1 (en) | Rna purification method | |
CA2178369C (en) | Process for the purification and crystallisation of iopamidol | |
JP2003306497A (ja) | 巨大分子結晶の製造方法及びそれに用いる製造装置 | |
CN109431996B (zh) | 一种含米诺地尔的外用剂组合物及其制备方法 | |
EP1062237B1 (de) | Metallhaltige ribonukleotidpolypeptide | |
EP3153522A1 (en) | Process for the purification of erythropoietin and darbepoetin alfa | |
JP2017531643A (ja) | ヘモペキシンでの治療のための組成物および方法 | |
RU2453331C1 (ru) | Способ получения высокоочищенного кристаллического инсулина любого происхождения | |
CN104958255A (zh) | 一种氟马西尼注射液及其制备方法 | |
CN110256533A (zh) | 一种高纯度的多臂抗癌偶联物 | |
TW202323269A (zh) | 方法 | |
CN116270454A (zh) | 盐酸丙卡特罗口服溶液及其制备方法 | |
DE102022121980A1 (de) | Neue Toxin-Bindemittel für die Hämodialyse | |
US20170101437A1 (en) | Process for purification of darbepoetin alfa | |
CN116251059A (zh) | 一种地高辛注射液的制备方法 | |
JP2006034113A (ja) | ホタテのウロのエキスの調製方法 | |
CN110934836A (zh) | 一种托吡司特滴丸及其制备方法 |