AU2020317617A1 - Detection of genomic sequences using combinations of probes, probe molecules and arrays comprising the probes for the specific detection of organisms - Google Patents

Detection of genomic sequences using combinations of probes, probe molecules and arrays comprising the probes for the specific detection of organisms Download PDF

Info

Publication number
AU2020317617A1
AU2020317617A1 AU2020317617A AU2020317617A AU2020317617A1 AU 2020317617 A1 AU2020317617 A1 AU 2020317617A1 AU 2020317617 A AU2020317617 A AU 2020317617A AU 2020317617 A AU2020317617 A AU 2020317617A AU 2020317617 A1 AU2020317617 A1 AU 2020317617A1
Authority
AU
Australia
Prior art keywords
probe
genome
virtual
seq
amplicon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU2020317617A
Other languages
English (en)
Inventor
Sonja Bednar
Holger Klapproth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Safeguard Biosystems Holdings Ltd
Original Assignee
Safeguard Biosystems Holdings Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Safeguard Biosystems Holdings Ltd filed Critical Safeguard Biosystems Holdings Ltd
Publication of AU2020317617A1 publication Critical patent/AU2020317617A1/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10Detection mode being characterised by the assay principle
    • C12Q2565/102Multiple non-interacting labels
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/501Detection characterised by immobilisation to a surface being an array of oligonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/16Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2020317617A 2019-07-19 2020-07-17 Detection of genomic sequences using combinations of probes, probe molecules and arrays comprising the probes for the specific detection of organisms Pending AU2020317617A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962876413P 2019-07-19 2019-07-19
US62/876,413 2019-07-19
US202063004664P 2020-04-03 2020-04-03
US63/004,664 2020-04-03
PCT/EP2020/070308 WO2021013736A1 (en) 2019-07-19 2020-07-17 Detection of genomic sequences using combinations of probes, probe molecules and arrays comprising the probes for the specific detection of organisms

Publications (1)

Publication Number Publication Date
AU2020317617A1 true AU2020317617A1 (en) 2022-03-03

Family

ID=71670264

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2020317617A Pending AU2020317617A1 (en) 2019-07-19 2020-07-17 Detection of genomic sequences using combinations of probes, probe molecules and arrays comprising the probes for the specific detection of organisms

Country Status (13)

Country Link
US (1) US20220267833A1 (zh)
EP (1) EP3999658A1 (zh)
JP (1) JP2022540716A (zh)
KR (1) KR20220035482A (zh)
CN (1) CN114269944A (zh)
AU (1) AU2020317617A1 (zh)
BR (1) BR112021026672A2 (zh)
CA (1) CA3147364A1 (zh)
IL (1) IL289760A (zh)
MX (1) MX2022000628A (zh)
TW (1) TW202117021A (zh)
WO (1) WO2021013736A1 (zh)
ZA (1) ZA202200284B (zh)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11562057B2 (en) 2020-02-05 2023-01-24 Quantum Digital Solutions Corporation Ecosystem security platforms for enabling data exchange between members of a digital ecosystem using digital genomic data sets
WO2022169969A1 (en) * 2021-02-04 2022-08-11 Quantum Digital Solutions Corporation Cyphergenics-based ecosystem security platforms
JP2024507719A (ja) 2021-02-04 2024-02-21 クアンタム デジタル ソリューションズ コーポレーション サイファージェニクスベースのエコシステムセキュリティプラットフォーム

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US6638719B1 (en) * 1999-07-14 2003-10-28 Affymetrix, Inc. Genotyping biallelic markers
US20030134293A1 (en) * 1999-11-16 2003-07-17 Zhiping Liu Method for rapid and accurate identification of microorganisms
US7205104B2 (en) * 2000-03-24 2007-04-17 Eppendorf Array Technologies Sa (Eat) Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays
CN1280422C (zh) 2004-08-26 2006-10-18 北京博奥生物芯片有限责任公司 一种不对称pcr扩增方法及其应用
EP2176281A4 (en) * 2007-07-11 2010-09-15 Univ Laval NUCLEIC ACID SEQUENCES AND COMBINATION THEREOF FOR SENSITIVE AMPLIFICATION AND SENSITIVE DETECTION OF BACTERIAL AND MIRACLE SEPSISPATHOGENESE
EP3181700A1 (en) 2015-12-18 2017-06-21 Safeguard Biosystems Holdings Ltd. Three-dimensional polymer networks with channels situated therein
US10036054B2 (en) 2016-01-30 2018-07-31 Safeguard Biosystems Holdings Ltd. Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms
EP3418741A1 (en) 2017-06-19 2018-12-26 Safeguard Biosystems Holdings Ltd. Three-dimensional polymer networks and their use
US10513730B2 (en) 2017-11-22 2019-12-24 Safeguard Biosystems Holdings Ltd. Asymmetric PCR methods, primers and kits

Also Published As

Publication number Publication date
BR112021026672A2 (pt) 2022-02-15
CN114269944A (zh) 2022-04-01
WO2021013736A1 (en) 2021-01-28
JP2022540716A (ja) 2022-09-16
MX2022000628A (es) 2022-03-11
IL289760A (en) 2022-03-01
ZA202200284B (en) 2022-09-28
TW202117021A (zh) 2021-05-01
US20220267833A1 (en) 2022-08-25
KR20220035482A (ko) 2022-03-22
CA3147364A1 (en) 2021-01-28
EP3999658A1 (en) 2022-05-25

Similar Documents

Publication Publication Date Title
US20220267833A1 (en) Detection of genomic sequences using combinations of probes, probe molecules and arrays comprising the probes for the specific detection of organisms
JP2013531983A (ja) 多重生物検出のための核酸ならびにその使用および製造方法
BRPI0517131B1 (pt) Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas
WO2014106076A2 (en) Universal sanger sequencing from next-gen sequencing amplicons
EP2344673B1 (en) Diagnostic test for streptococcus equi
WO2013173774A2 (en) Molecular inversion probes
US20080228406A1 (en) System and method for fungal identification
JP5916740B2 (ja) 核酸標的の定量的多重同定
US20210017582A1 (en) Detection of genomic sequences and probe molecules therefor
CN113136443B (zh) 一种快速鉴定蜡样芽胞杆菌和苏云金芽胞杆菌的核酸检测方法
WO2022157248A1 (en) Improved detection of genomic sequences and probe molecules therefor
WO2022157672A1 (en) Improved detection of genomic sequences and probe molecules therefor
CN101092645B (zh) 诊断性传染病致病原的新颖方法
US8304184B2 (en) Genotyping using multiple variant-specific primer pools
KR20100012319A (ko) 패혈증­유발 미생물의 분류 및 동정 방법
Nazir Polymerase Chain Reaction
WO2024030342A1 (en) Methods and compositions for nucleic acid analysis
EP4058605A1 (en) Method of determining the presence of a hyper-virulent clostridioides difficile strain of the b1/nap1/027 group in a sample
JP2023511909A (ja) 細菌定量化方法
CN116356049A (zh) 一种检测艰难梭状芽孢杆菌的试剂盒
CN116356075A (zh) 一种用于检测的引物探针、引物探针组及其应用
BR102014008162A2 (pt) método de identificação de espécies bacterianas e kit para o diagnóstico de bactérias