AU2020279663B2 - Ribulose-Phosphate 3-Epimerase Motif Having Low Side Reactivity And Enzyme Including The Same - Google Patents

Ribulose-Phosphate 3-Epimerase Motif Having Low Side Reactivity And Enzyme Including The Same Download PDF

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AU2020279663B2
AU2020279663B2 AU2020279663A AU2020279663A AU2020279663B2 AU 2020279663 B2 AU2020279663 B2 AU 2020279663B2 AU 2020279663 A AU2020279663 A AU 2020279663A AU 2020279663 A AU2020279663 A AU 2020279663A AU 2020279663 B2 AU2020279663 B2 AU 2020279663B2
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phosphate
epimerase
ribulose
psicose
amino acid
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AU2020279663A1 (en
Inventor
Hyun Kug Cho
Seong Bo Kim
Seung Hwan Kim
Hyun June Park
Il Hyang PARK
Byung-Sam Son
Sungjae YANG
Sang Young Yoon
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CJ CheilJedang Corp
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CJ CheilJedang Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03001—Ribulose-phosphate 3-epimerase (5.1.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2020279663A 2019-05-21 2020-04-29 Ribulose-Phosphate 3-Epimerase Motif Having Low Side Reactivity And Enzyme Including The Same Active AU2020279663B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020190059697A KR102080886B1 (ko) 2019-05-21 2019-05-21 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
KR10-2019-0059697 2019-05-21
PCT/KR2020/005701 WO2020235830A1 (ko) 2019-05-21 2020-04-29 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소

Publications (2)

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AU2020279663A1 AU2020279663A1 (en) 2021-09-30
AU2020279663B2 true AU2020279663B2 (en) 2023-04-13

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AU2020279663A Active AU2020279663B2 (en) 2019-05-21 2020-04-29 Ribulose-Phosphate 3-Epimerase Motif Having Low Side Reactivity And Enzyme Including The Same

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US (1) US12371686B2 (enExample)
EP (1) EP3916089A4 (enExample)
JP (2) JP7410162B2 (enExample)
KR (1) KR102080886B1 (enExample)
CN (1) CN114008198B (enExample)
AU (1) AU2020279663B2 (enExample)
WO (1) WO2020235830A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102080886B1 (ko) 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
JP2023500269A (ja) * 2019-10-28 2023-01-05 サムヤン コーポレイション フルクトース－６－リン酸３－エピマー化酵素およびその用途
KR20230012047A (ko) * 2020-05-18 2023-01-25 보너모스, 인코포레이티드 알룰로스의 효소적 생산
KR20250154456A (ko) * 2023-02-28 2025-10-28 카아길, 인코포레이팃드 D-알룰로스의 생산을 위한 유전자 변형 미생물 및 발효 공정

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003084986A2 (en) * 2002-04-04 2003-10-16 Affinium Pharmaceuticals, Inc. Novel purified polypeptides involved in cellular metabolism
WO2004042043A2 (en) * 2002-11-05 2004-05-21 Affinium Pharmaceuticals, Inc. Crystal structures of bacterial ribulose-phosphate 3-epimerases
US20160060635A1 (en) * 2013-03-14 2016-03-03 The Regents Of The University Of California Recombinant microorganisms having a methanol elongation cycle (mec)
WO2018132871A1 (en) * 2017-01-17 2018-07-26 Macquarie University Method of producing chemicals in yeast
WO2019027173A2 (ko) * 2017-07-31 2019-02-07 씨제이제일제당(주) 신규한 싸이코스-6-인산 탈인산효소, 상기 효소를 포함하는 사이코스 생산용 조성물, 상기 효소를 이용하여 사이코스를 제조하는 방법

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SE0200857D0 (sv) * 2002-03-19 2002-03-19 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of saccharomyces cerevisiae
KR101473918B1 (ko) * 2014-05-28 2014-12-17 대상 주식회사 사이코스 에피머화 효소, 이의 제조방법 및 이를 이용한 사이코스의 제조방법
BR112018077188B1 (pt) * 2016-06-30 2022-09-06 Cj Cheiljedang Corporation Método e composição compreendendo frutose-6-fosfato-3-epimerase termoestável para produzir alulose
CN119286951A (zh) * 2016-12-14 2025-01-10 博努莫斯有限责任公司 D-阿洛酮糖的酶法生产
WO2018129275A1 (en) 2017-01-06 2018-07-12 Greenlight Biosciences, Inc. Cell-free production of sugars
KR101965509B1 (ko) * 2017-11-15 2019-04-03 씨제이제일제당 (주) 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법
KR102080886B1 (ko) * 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003084986A2 (en) * 2002-04-04 2003-10-16 Affinium Pharmaceuticals, Inc. Novel purified polypeptides involved in cellular metabolism
WO2004042043A2 (en) * 2002-11-05 2004-05-21 Affinium Pharmaceuticals, Inc. Crystal structures of bacterial ribulose-phosphate 3-epimerases
US20160060635A1 (en) * 2013-03-14 2016-03-03 The Regents Of The University Of California Recombinant microorganisms having a methanol elongation cycle (mec)
WO2018132871A1 (en) * 2017-01-17 2018-07-26 Macquarie University Method of producing chemicals in yeast
WO2019027173A2 (ko) * 2017-07-31 2019-02-07 씨제이제일제당(주) 신규한 싸이코스-6-인산 탈인산효소, 상기 효소를 포함하는 사이코스 생산용 조성물, 상기 효소를 이용하여 사이코스를 제조하는 방법

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHAN ET AL.: "Structural Basis for Substrate Specificity in Phosphate Binding (β/α)8-Barrels: d-Allulose 6-Phosphate 3-Epimerase from Escherichia coli K-12", BIOCHEMISTRY, vol. 47, 1 January 2008 (2008-01-01), pages 9608 - 9617, XP055555262 *
NCBI: "ribulose-phosphate 3-epimerase [Thermoflavimicrobium dichotomicum]", GENBANK ACCESSION NO.WP_093231204, JPN6022030671, 1 August 2017 (2017-08-01), ISSN: 0004835513 *

Also Published As

Publication number Publication date
KR102080886B1 (ko) 2020-02-24
JP2022522693A (ja) 2022-04-20
AU2020279663A1 (en) 2021-09-30
EP3916089A4 (en) 2022-05-11
US20230051519A1 (en) 2023-02-16
CN114008198A (zh) 2022-02-01
JP7566993B2 (ja) 2024-10-15
WO2020235830A1 (ko) 2020-11-26
EP3916089A1 (en) 2021-12-01
JP7410162B2 (ja) 2024-01-09
CN114008198B (zh) 2024-06-07
US12371686B2 (en) 2025-07-29
JP2023166380A (ja) 2023-11-21

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Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE INVENTION TITLE TO READ RIBULOSE-PHOSPHATE 3-EPIMERASE MOTIF HAVING LOW SIDE REACTIVITY AND ENZYME INCLUDING THE SAME

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