JP7410162B2 - 副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素 - Google Patents

副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素 Download PDF

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JP7410162B2
JP7410162B2 JP2021549965A JP2021549965A JP7410162B2 JP 7410162 B2 JP7410162 B2 JP 7410162B2 JP 2021549965 A JP2021549965 A JP 2021549965A JP 2021549965 A JP2021549965 A JP 2021549965A JP 7410162 B2 JP7410162 B2 JP 7410162B2
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phosphate
psicose
motif
seq
amino acid
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JP2022522693A (ja
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ヨン ユン，サン
ソン，ピョン－サム
クク チョ，ヒョン
チョン パク，ヒョン
ファン キム，ソン
ヤン，ソンチェ
ヒャン パク，イル
ボ キム，ソン
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CJ CheilJedang Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y501/00—Racemaces and epimerases (5.1)
    • C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
    • C12Y501/03001—Ribulose-phosphate 3-epimerase (5.1.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
JP2021549965A 2019-05-21 2020-04-29 副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素 Active JP7410162B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023128141A JP7566993B2 (ja) 2019-05-21 2023-08-04 副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020190059697A KR102080886B1 (ko) 2019-05-21 2019-05-21 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
KR10-2019-0059697 2019-05-21
PCT/KR2020/005701 WO2020235830A1 (ko) 2019-05-21 2020-04-29 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소

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JP2023128141A Division JP7566993B2 (ja) 2019-05-21 2023-08-04 副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素

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JP2022522693A JP2022522693A (ja) 2022-04-20
JP7410162B2 true JP7410162B2 (ja) 2024-01-09

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JP2021549965A Active JP7410162B2 (ja) 2019-05-21 2020-04-29 副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素
JP2023128141A Active JP7566993B2 (ja) 2019-05-21 2023-08-04 副反応性が低いリブロースリン酸３－エピメラーゼのモチーフ及びそれを含む酵素

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US (1) US12371686B2 (enExample)
EP (1) EP3916089A4 (enExample)
JP (2) JP7410162B2 (enExample)
KR (1) KR102080886B1 (enExample)
CN (1) CN114008198B (enExample)
AU (1) AU2020279663B2 (enExample)
WO (1) WO2020235830A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102080886B1 (ko) * 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소
WO2021086010A1 (ko) * 2019-10-28 2021-05-06 주식회사 삼양사 과당-6-인산 3-에피머화 효소 및 이의 용도
WO2021236632A2 (en) * 2020-05-18 2021-11-25 Bonumose, Inc. Enzymatic production of allulose
WO2024182327A1 (en) * 2023-02-28 2024-09-06 Cargill, Incorporated Genetically modified microorganism and fermentation process for the production of d-allulose

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003084986A2 (en) 2002-04-04 2003-10-16 Affinium Pharmaceuticals, Inc. Novel purified polypeptides involved in cellular metabolism
WO2004042043A2 (en) 2002-11-05 2004-05-21 Affinium Pharmaceuticals, Inc. Crystal structures of bacterial ribulose-phosphate 3-epimerases
WO2018132871A1 (en) 2017-01-17 2018-07-26 Macquarie University Method of producing chemicals in yeast
KR101965509B1 (ko) 2017-11-15 2019-04-03 씨제이제일제당 (주) 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE0200857D0 (sv) * 2002-03-19 2002-03-19 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of saccharomyces cerevisiae
US10006033B2 (en) 2013-03-14 2018-06-26 The Regents Of The University Of California Recombinant microorganisms having a methanol elongation cycle (MEC)
KR101473918B1 (ko) * 2014-05-28 2014-12-17 대상 주식회사 사이코스 에피머화 효소, 이의 제조방법 및 이를 이용한 사이코스의 제조방법
CN109563499B (zh) * 2016-06-30 2022-08-12 Cj第一制糖株式会社 一种新型热稳定果糖-6-磷酸盐-3-差向异构酶和使用其生产阿洛糖的方法
EP3555282A4 (en) 2016-12-14 2020-07-22 Bonumose LLC ENZYMATIC PRODUCTION OF D-ALLULOSE
AU2018205503A1 (en) 2017-01-06 2019-07-25 Greenlight Biosciences, Inc. Cell-free production of sugars
JP6973898B2 (ja) * 2017-07-31 2021-12-01 シージェイ チェイルジェダン コーポレーションCj Cheiljedang Corporation 新規のプシコース−６−リン酸脱リン酸酵素、前記酵素を含むプシコース生産用組成物、前記酵素を利用してプシコースを製造する方法
KR102080886B1 (ko) * 2019-05-21 2020-02-24 씨제이제일제당 주식회사 부반응성이 낮은 리불로스-인산 3-에피머화 효소의 모티프 및 이를 포함하는 효소

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003084986A2 (en) 2002-04-04 2003-10-16 Affinium Pharmaceuticals, Inc. Novel purified polypeptides involved in cellular metabolism
WO2004042043A2 (en) 2002-11-05 2004-05-21 Affinium Pharmaceuticals, Inc. Crystal structures of bacterial ribulose-phosphate 3-epimerases
WO2018132871A1 (en) 2017-01-17 2018-07-26 Macquarie University Method of producing chemicals in yeast
KR101965509B1 (ko) 2017-11-15 2019-04-03 씨제이제일제당 (주) 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NCBI，ribulose-phosphate 3-epimerase [Thermoflavimicrobium dichotomicum]，GenBank Accession No.WP_093231204，2017年08月01日，htpps://www.ncbi.nlm.nih.gov/protein/WP_093231204.1/

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Publication number Publication date
JP7566993B2 (ja) 2024-10-15
CN114008198B (zh) 2024-06-07
KR102080886B1 (ko) 2020-02-24
EP3916089A1 (en) 2021-12-01
US12371686B2 (en) 2025-07-29
CN114008198A (zh) 2022-02-01
JP2022522693A (ja) 2022-04-20
AU2020279663A1 (en) 2021-09-30
AU2020279663B2 (en) 2023-04-13
JP2023166380A (ja) 2023-11-21
US20230051519A1 (en) 2023-02-16
EP3916089A4 (en) 2022-05-11
WO2020235830A1 (ko) 2020-11-26

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