AU2020101984A4 - A Kind of Culture Medium for Promoting Rooting of Hylotelephium spectabile (Boreau) H. Ohba in Hydroponic Culture - Google Patents
A Kind of Culture Medium for Promoting Rooting of Hylotelephium spectabile (Boreau) H. Ohba in Hydroponic Culture Download PDFInfo
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- 241000218092 Hylotelephium spectabile Species 0.000 title claims abstract description 31
- 230000001737 promoting effect Effects 0.000 title claims abstract description 12
- 239000001963 growth medium Substances 0.000 title claims description 9
- 238000005520 cutting process Methods 0.000 claims abstract description 26
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims abstract description 10
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000015097 nutrients Nutrition 0.000 claims abstract description 8
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical class C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims abstract description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000004327 boric acid Substances 0.000 claims abstract description 6
- 239000004062 cytokinin Substances 0.000 claims abstract description 6
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims abstract description 6
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims abstract description 6
- MKWYFZFMAMBPQK-UHFFFAOYSA-J sodium feredetate Chemical compound [Na+].[Fe+3].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O MKWYFZFMAMBPQK-UHFFFAOYSA-J 0.000 claims abstract description 6
- RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 claims abstract description 6
- ZHJGWYRLJUCMRT-UHFFFAOYSA-N 5-[6-[(4-methylpiperazin-1-yl)methyl]benzimidazol-1-yl]-3-[1-[2-(trifluoromethyl)phenyl]ethoxy]thiophene-2-carboxamide Chemical compound C=1C=CC=C(C(F)(F)F)C=1C(C)OC(=C(S1)C(N)=O)C=C1N(C1=C2)C=NC1=CC=C2CN1CCN(C)CC1 ZHJGWYRLJUCMRT-UHFFFAOYSA-N 0.000 claims abstract description 5
- QGAVSDVURUSLQK-UHFFFAOYSA-N ammonium heptamolybdate Chemical compound N.N.N.N.N.N.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.O.[Mo].[Mo].[Mo].[Mo].[Mo].[Mo].[Mo] QGAVSDVURUSLQK-UHFFFAOYSA-N 0.000 claims abstract description 5
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims abstract description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 5
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000001103 potassium chloride Substances 0.000 claims abstract description 5
- 235000011164 potassium chloride Nutrition 0.000 claims abstract description 5
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 claims abstract description 4
- 230000000052 comparative effect Effects 0.000 claims description 33
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 12
- AJTPODXVRWXEMS-UHFFFAOYSA-M potassium;4-(1h-indol-2-yl)butanoate Chemical group [K+].C1=CC=C2NC(CCCC(=O)[O-])=CC2=C1 AJTPODXVRWXEMS-UHFFFAOYSA-M 0.000 claims description 9
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims description 7
- CJUUXVFWKYRHAR-UHFFFAOYSA-M 1-Naphthaleneacetic acid sodium salt Chemical group [Na+].C1=CC=C2C(CC(=O)[O-])=CC=CC2=C1 CJUUXVFWKYRHAR-UHFFFAOYSA-M 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 2
- CONKBQPVFMXDOV-QHCPKHFHSA-N 6-[(5S)-5-[[4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]piperazin-1-yl]methyl]-2-oxo-1,3-oxazolidin-3-yl]-3H-1,3-benzoxazol-2-one Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)N1CCN(CC1)C[C@H]1CN(C(O1)=O)C1=CC2=C(NC(O2)=O)C=C1 CONKBQPVFMXDOV-QHCPKHFHSA-N 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000012452 mother liquor Substances 0.000 description 9
- 239000002689 soil Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 5
- 239000011591 potassium Substances 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- PRPINYUDVPFIRX-UHFFFAOYSA-M 1-naphthaleneacetate Chemical compound C1=CC=C2C(CC(=O)[O-])=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-M 0.000 description 2
- 241000220284 Crassulaceae Species 0.000 description 2
- 229910052793 cadmium Inorganic materials 0.000 description 2
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- FRASJONUBLZVQX-UHFFFAOYSA-N 1,4-naphthoquinone Chemical compound C1=CC=C2C(=O)C=CC(=O)C2=C1 FRASJONUBLZVQX-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- 241000054822 Lycaena cupreus Species 0.000 description 1
- 235000009071 Mesembryanthemum crystallinum Nutrition 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 239000003501 hydroponics Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 239000003226 mitogen Substances 0.000 description 1
- 150000004682 monohydrates Chemical class 0.000 description 1
- XPGDBEXDXZQNFP-UHFFFAOYSA-N nitrate tetrahydrate Chemical compound O.O.O.O.[O-][N+]([O-])=O XPGDBEXDXZQNFP-UHFFFAOYSA-N 0.000 description 1
- 150000004686 pentahydrates Chemical class 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 239000012883 rooting culture medium Substances 0.000 description 1
- 239000012882 rooting medium Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- KUNICNFETYAKKO-UHFFFAOYSA-N sulfuric acid;pentahydrate Chemical compound O.O.O.O.O.OS(O)(=O)=O KUNICNFETYAKKO-UHFFFAOYSA-N 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/32—Crassulaceae
- A01H6/328—Sedum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/02—Flowers
Abstract
The invention discloses a culture liquid for promoting cutting rooting of Hylotelephium
spectabile (Boreau) H. Ohba in water culture condition, which comprises the following
components: a plant growth regulator, a basic nutrient solution. The plant growth regulator
comprises an auxin analogue, a cytokinin analogue and, the basic nutrient solution comprises
calcium nitrate tetrahydrate, potassium chloride, potassium dihydrogen phosphate, magnesium
sulphate heptahydrate, boric acid, manganese sulphate monohydrate, zinc sulphate
heptahydrate, copper sulphate pentahydrate, ammonium heptamolybdate, and sodium iron
EDTA. The culture liquid for promoting the cutting rooting of H. spectabile in water culture
provided by the invention can greatly improve the rooting rate and the root amount of the
cutting, which solves the key technical problem of the rapid propagation of the H. spectabile,
and is of great significance to the rapid propagation and industrial seedling production of H.
spectabile.
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Description
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PATENTS ACT 1990
A Kind of Culture Medium for Promoting Rooting of Hylotelephium spectabile (Boreau)
H. Ohba in Hydroponic Culture
The invention is described in the following statement:-
A Kind of Culture Medium for Promoting Rooting of Hylotelephium spectabile
(Boreau) H. Ohba in Hydroponic Culture
The invention belongs to the technical field of plant culture liquid, in particular to a
culture liquid for promoting rooting of Hylotelephium spectabile (Boreau) H. Ohba in
hydroponic culture.
Hylotelephium spectabile (Boreau) H. Ohba, formerly called Sedum Spectabile
Boreau, is an herbaceous perennial plant in the Crassulaceae family with highly
ornamental value and is widely cultivated in China and Korea. Its common names include
showy stonecrop, ice plant, and butterfly stonecrop. The underground stem of H.
spectabile is thick and fleshy, and the aboveground stem is clumped, thick and erect. The
plant height of H. spectabile can reach 50 cm. It has alternate, simple, toothed leaves on
erect and unbranched succulent stems. The flower of H. spectabile is star-shaped, and can
be pink, white, purplish red and rose red which is the most colourful species in
Crassulaceae family. Therefore, it is commonly used to arrange flowerbeds and flower
borders with other flowers or to be planted in patches for protecting slope land, and has a
broad application prospect in the field of garden greening.
At the same time, in recent years, soil heavy metal pollution has become a serious
environmental problem in the world. At present, the farmland soil in China is seriously
polluted by heavy metals. According to the Investigation Report of Soil Pollution
Condition in China, the point exceeding rate of contaminants of cultivated land is 19.4%,
wherein the proportion of mild, light, medium and heavy pollution sites is 13.7%, 2.8%,
1.8% and 1.1% respectively. While from the point of excessive pollutants, the over
standard rate of heavy metal cadmium is 7.0%. About 25 million hectares of farmland
soil are polluted by heavy metals in China, and as much as 12 million tons of grain is
polluted by the heavy metals every year, causing direct economic losses of more than 10
billion yuan. H. spectabile has strong accumulation capacity of cadmium in soil and
shows the potential for remediation of Cd-contaminated soil. Furthermore, H. spectabile
is fast growing with large biomass and could adapt to different climate and soil conditions
such as arid, cold and barren. Therefore, it has a broad prospect in phytoremediation of
Cd-contaminated soils with the advantages of low cost, small disturbance to the
environment, no secondary pollution and strong sustainability. In addition, it could also
beautify the environment.
In view of the broad application prospects of H. spectabile, its seedling demand has
also increased gradually. Sowing directly is tedious and take a long time for seedling
formation and cutting propagation technology is often used in practical application,
however, because of the slow rooting of direct cutting, the growth and propagation of H.
spectabile is slow, which affects its wide range of promotion and planting. Therefore, the
key technical problem of rapid propagation of H. spectabile is rapid rooting, which will
be of great significance for the rapid reproduction and industrialized seedling production
of the plant.
In view of the above, the present invention aims to provide a culture medium for
promoting the rooting of H. spectabile seedlings in water culture condition so as to solve
the technical problems in the prior art.
In order to solve the above technical problems, the present invention provides the
following technical solutions.
A culture liquid for promoting cutting rooting of H. spectabile in water culture
condition comprises the following components: a plant growth regulator, a basic nutrient
solution. The plant growth regulator comprises an auxin analogue, a cytokinin analogue
and, the basic nutrient solution comprises calcium nitrate tetrahydrate, potassium
chloride, potassium dihydrogen phosphate, magnesium sulphate heptahydrate, boric acid,
manganese sulphate monohydrate, zinc sulphate heptahydrate, copper sulphate
pentahydrate, ammonium heptamolybdate, and sodium iron EDTA.
Further, the mass ratio of the auxin analogue to the cytokinin analogue is 1: 1.
Further, the auxin analogue is sodium naphthalene-1-acetate and the mitogen
analogue is potassium indolebutyrate.
Further, the concentrations of sodium naphthalene-1-acetate and indolebutyrate
potassium in the culture solution were both 0.2 mg / L.
Further, the potassium is prepared by reaction of indolebutyric acid with potassium
hydroxide, specifically, taking the indolebutyric acid and potassium hydroxide in a molar
amount ratio of 1: 1, dissolving them in water, stirring, and reacting to obtain the
potassium indolebutyrate.
In addition, the concentration of calcium nitrate tetrahydrate and potassium chloride
is 2.5 mmol / L, and that of potassium dihydrogen phosphate is 0.5 mmol / L, of
magnesium sulphate heptahydrate is 0.5 mmol / L, of boric acid is 25 mol / L, that of
manganese sulphate monohydrate is 2.25 mol / L, that of zinc sulphate heptahydrate is
1.9 mol / L, of copper sulphate pentahydrate is 0.15 mol / L, that of ammonium
heptamolybdate is 0.05 mmol / L and that of sodium iron EDTA is 5 mol / L.
In comparison with traditional technology, the beneficial effects of the invention are
as follows.
(1) The culture solution for promoting the rooting of cuttings of H. spectabile in the
water culture provided by the invention can greatly improve the rooting rate and the
rooting amount of the cuttings, and solve the key technical problem of the rapid
propagation of H. spectabile; it is of great significance for the rapid reproduction and
industrialized seedling production of the plant.
In order to more clearly explain that embodiments of the present invention or the
technical solution in the prior art, the following will briefly introduce the drawings
needed in the embodiments. Obviously, the drawings described below are only some
embodiments of the invention. For ordinary technical personnel in the art, they can also
be obtained according to these drawings without paying creative labour other figures.
Fig. 1 shows the picture of rooting results obtained after one week of hydroponic cutting
rooting test of H. spectabile with the medium of embodiment example 1 and the
comparative examples 1-9.
Various exemplary embodiments of the present invention are described in detail,
which should not be construed as a limitation of the invention, but should be understood
as a more detailed description of certain aspects, characteristics and embodiments of the
invention. It should be understood that the terms described in the present invention are
only for describing particular embodiments and are not intended to limit the invention.
In addition, for the numerical ranges in the present invention, it should be
understood that each intermediate value between the upper and lower limits of the range
is also specifically disclosed. Each smaller range between any stated value or
intermediate value within the stated range and any other stated value or intermediate
value within said range is also included in the present invention. The upper and lower
limits of these smaller ranges can be included or excluded independently.
Unless otherwise specified, all technical and scientific terms used herein have the
same meaning as those of ordinary skill in the art of the invention. Although only
preferred methods and materials have been described herein, any method and materials
similar or equivalent to those described herein may be used in the practice or testing of
the present invention. All references referred to in this specification are incorporated by
reference to disclose and describe methods and/or materials related to the literature. In
case of conflict with any incorporated literature, the contents of this specification shall
prevail.
Without departing from the scope or spirit of the invention, various improvements
and changes can be made to the specific embodiment of the specification of the invention, which is obvious to those skilled in the art. Other embodiments obtained from the description of the invention are obvious to the technical personnel. The description and embodiment of the invention are only illustrative.
The terms "embodying", "including", "having", "containing" and so on used in this
paper are all open terms, which means including but not limited to.
The "parts" mentioned in the present invention are all calculated by weight unless
otherwise specified.
Embodiment example 1
The preparation of the culture medium for promoting the rooting of cuttings of H.
spectabile in hydroponic culture.
(1) Preparation of basic nutrient solution, mother liquor 1 (0.25 mol / L calcium
nitrate tetrahydrate), mother liquor 2 (0.05 mol / L magnesium sulphate heptahydrate,
0.25 mol / L potassium chloride), and mother liquor 3 (0.025 mol / L potassium
dihydrogen phosphate), and mother liquor four (0.01125 mol / L manganese sulphate
monohydrate, 0.0095 mol / L zinc sulphate heptahydrate, and 0.00075 mol / L. copper
sulphate pentahydrate), and mother liquor 5 (0.025 mol / 1 boric acid, 0.05 mol / L
ammonium heptamolybdate) and mother liquor 6 (0.0125 mol / L sodium iron EDTA).
(2) Preparation of plant growth regulator mother liquor: 0.1 g sodium naphthalene-1
acetate is dissolved in 1 L of ultrapure water to obtain 0.1 g / L mother liquor of sodium
naphthalene-1-acetate; 0.0842 g indole butyric acid and 0.0232 g potassium hydroxide
are dissolved in water, and then the volume is fixed to 1 L to obtain 0.1 g / L mother
liquor of potassium indolebutyrate.
(3) Take 10 mL, 10 mL, 20 mL, 0.2 mL, 1 mL and 0.4 mL of basic nutrient solution
prepared in step (1) respectively, and then take some mother liquor of plant growth
regulator sodium naphthalene-1-acetate and potassium indolebutyrate prepared in step (2)
to make their final concentration be 0.2 mg / L and 0.2 mg / L, the components are fully
mixed and diluted to 1 L, so as to obtain the culture medium for promoting the cutting
rooting of H. spectabile in hydroponic culture.
Comparative examples 1-9
Comparative examples 1-9 are the same as that of embodiment example 1. The
difference is that the proportion and concentration of plant growth regulators in the
culture medium are different, as shown in Table 1.
Table 1
Final concentration of sodium Final concentration of potassium Group naphthalene-1-acetate (mg / L) indolebutyrate (mg / L)
Comparative example 1 0 0
Comparative example 2 0.1 0.1
Comparative example 3 0.1 0.3
Comparative example 4 0.1 0.5
Comparative example 5 0.2 0.6
Comparative example 6 0.2 1.0
Comparative example 7 0.3 0.3
Comparative example 8 0.3 0.9
Comparative example 9 0.3 1.5
Effect verification example
The experiment method of indoor hydroponics was used to carry out the cutting
rooting test of H. spectabile with the culture solution prepared by embodiment example 1
and the comparative examples 1-9. The specific test methods are as follows.
Choose a IL transparent plastic basin as the culture container. The container is
wrapped with tin foil to prevent the growth of green algae. Soak it with 0.15%-0.3% 84
disinfectant for 25-40 minutes, and rinse with clean water for later use. Use the
polyethylene foam board with 1 cm thick as the cutting board for the cuttings of H.
spectabile. The cutting board was cut to the size of the bottle mouth and each cutting
board has 4 holes of about 0.5 cm diameter for cutting branches.
Forty healthy branches of H. spectabile were selected and cut into 8-10 cm long.
The head of the cutting is flattened, and the bottom is cut into a horseshoe shape.
Add 2/3 volume (about 600 mL) of rooting medium into the culture vessel and plant
4 cuttings of H. spectabile into each cuttage board. About 1/3 long of the cutting was
planted into the holes of the board and then fixed with a sponge. The cuttage board was
then placed into the vessel to make sure that the 2-3 cm base section of cuttings
accessible to the rooting culture medium. The cuttings were cultivated at 18-25 °C and
observed the rooting situation continuously. The start date of rooting for each pot was
recorded. The rooting start dates of each group was shown in table 2 and the rooting
situation of each group after one week was shown in figure 1.
Table 2
Group Rooting start date Embodiment example 1 Day 5 Comparative example 1 Unrooted Comparative example 2 Day 6 Comparative example 3 Day 7 Comparative example 4 Day 7 Comparative example 5 Unrooted Comparative example 6 Day 7 Comparative example 7 Unrooted Comparative example 8 Unrooted Comparative example 9 Day 6
The results showed that under the conditions of the embodiment example, the
cutting segment of H. spectabile began rooting on the 5th day, and the roots were very
abundant after being cultured for 20 days, and the cuttings grew healthily and fast after
transplanted into soil.
The cutting rooting results of embodiment example and other comparative examples
after being cultured for one week are shown in Figure 1. After culturing for 20 days, the
rooting amount in embodiment example was the most abundant and the root length was
the longest, which meant the optimal ratio of sodium naphthalene-1-acetate and
potassium indolebutyrate in the rooting culture solution was 1:1, and the optimal added
concentration was 0.2 mg/L and 0.2 mg/L. The results of remaining comparative
examples showed that the hydroponic rooting of H. spectabile was sensitive to the ratio
and concentration of plant growth regulators, and small adjustments can significantly
affect the test results.
The above are only the preferred specific embodiments of the present invention, but
the protection scope of the present invention is not limited to this. Anyone familiar with
the technical field in the technical scope disclosed in the present invention, according to the technical solution of the present invention equivalent replacements or changes to its inventive concept should all fall within the protection scope of the present invention.
Claims (6)
1. A culture liquid for promoting cutting rooting of Hylotelephium spectabile
(Boreau) H. Ohba in hydroponic culture, the culture comprises the following
components: a plant growth regulator and a basic nutrient solution. The plant growth
regulator comprises an auxin analogue, a cytokinin analogue. The basic nutrient solution
comprises calcium nitrate tetrahydrate, potassium chloride, potassium dihydrogen
phosphate, magnesium sulphate heptahydrate, boric acid, manganese sulphate
monohydrate, zinc sulphate heptahydrate, copper sulphate pentahydrate, ammonium
heptamolybdate, and sodium iron EDTA.
2. The culture solution according to claim 1 is characterized in that the mass ratio of
the auxin analogue to the cytokinin analogue is 1: 1.
3. The culture medium according to claim 1 is characterized in that the auxin
analogue is sodium naphthalene-1-acetate and the cytokinin analogue is potassium
indolebutyrate.
4. The culture medium according to claim 3 is characterized in that the
concentration of each sodium naphthalene-1-acetate and potassium indolebutyrate in the
culture liquid is 0.2 mg / L.
5. The culture solution according to claim 4 is characterized in that the potassium
indolebutyrate is prepared by reaction of indolebutyric acid with potassium hydroxide,
and in particular, the potassium hydroxide and indolebutyric acid are dissolved in water
in a molar amount ratio of 1: 1. Stirring and reacting to obtain the potassium
indolebutyrate.
6. The culture solution according to claim 1 is characterized in that the concentration of calcium nitrate tetrahydrate and potassium chloride is 2.5 mmol / L, and that of potassium dihydrogen phosphate is 0.5 mmol / L, of magnesium sulphate heptahydrate is
0.5 mmol / L, of boric acid is 25 mol / L, that of manganese sulphate monohydrate is
2.25 mol / L, that of zinc sulphate heptahydrate is 1.9 mol / L, of copper sulphate
pentahydrate is 0.15 mol / L, that of ammonium heptamolybdate was 0.05 mmol / L and
that of sodium iron EDTA is 5 mol / L.
Comparative example 9 Comparative example 8 Comparative example 7 Comparative example 6
Figure 1 Comparative example 5 -1/1-
Embodiment example 1 Comparative example 4 Comparative example 3 Comparative example 2 Comparative example 1 Aug 2020 2020101984
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114982559A (en) * | 2022-04-20 | 2022-09-02 | 侯羽 | Radical planting mate and using method thereof |
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2020
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| CN114982559A (en) * | 2022-04-20 | 2022-09-02 | 侯羽 | Radical planting mate and using method thereof |
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