AU2017225350B2 - Promoter - Google Patents

Promoter Download PDF

Info

Publication number
AU2017225350B2
AU2017225350B2 AU2017225350A AU2017225350A AU2017225350B2 AU 2017225350 B2 AU2017225350 B2 AU 2017225350B2 AU 2017225350 A AU2017225350 A AU 2017225350A AU 2017225350 A AU2017225350 A AU 2017225350A AU 2017225350 B2 AU2017225350 B2 AU 2017225350B2
Authority
AU
Australia
Prior art keywords
seq
nucleic acid
polynucleotide
acid molecule
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2017225350A
Other languages
English (en)
Other versions
AU2017225350A1 (en
Inventor
Ryan Cawood
Richard PARKER-MANUEL
Weiheng Su
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford Genetics Ltd
Original Assignee
Oxford Genetics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Genetics Ltd filed Critical Oxford Genetics Ltd
Publication of AU2017225350A1 publication Critical patent/AU2017225350A1/en
Application granted granted Critical
Publication of AU2017225350B2 publication Critical patent/AU2017225350B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2017225350A 2016-03-01 2017-02-28 Promoter Active AU2017225350B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1603577.6 2016-03-01
GBGB1603577.6A GB201603577D0 (en) 2016-03-01 2016-03-01 Promoter
PCT/GB2017/050537 WO2017149292A1 (en) 2016-03-01 2017-02-28 Promoter

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2023203856 Division 2017-02-28

Publications (2)

Publication Number Publication Date
AU2017225350A1 AU2017225350A1 (en) 2018-09-06
AU2017225350B2 true AU2017225350B2 (en) 2023-07-20

Family

ID=55807149

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2017225350A Active AU2017225350B2 (en) 2016-03-01 2017-02-28 Promoter

Country Status (9)

Country Link
US (1) US10647999B2 (enExample)
EP (2) EP3423583B1 (enExample)
JP (1) JP6960409B2 (enExample)
KR (1) KR102755545B1 (enExample)
CN (1) CN109072251A (enExample)
AU (1) AU2017225350B2 (enExample)
GB (3) GB201603577D0 (enExample)
SG (1) SG11201806782QA (enExample)
WO (1) WO2017149292A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110892064A (zh) 2017-07-25 2020-03-17 牛津遗传学有限公司 腺病毒载体
CN107630035A (zh) * 2017-09-28 2018-01-26 兰州大学 一种大鼠防御素真核表达载体及其构建方法
GB201800903D0 (en) 2018-01-19 2018-03-07 Oxford Genetics Ltd Vectors
BR112020015798A2 (pt) 2018-02-01 2021-03-09 Homology Medicines, Inc. Composições de vírus adeno-associado para restaurar função de gene da pah e métodos de uso das mesmas
GB201901571D0 (en) 2019-02-05 2019-03-27 Oxford Genetics Ltd Inducible AAV sysyem
TW202208632A (zh) 2020-05-27 2022-03-01 美商同源醫藥公司 用於恢復ｐａｈ基因功能的腺相關病毒組成物及其使用方法
AU2022373653A1 (en) 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007081336A1 (en) * 2006-01-13 2007-07-19 Five Prime Therapeutics, Inc. Mammalian vectors for high-level expression of recombinant proteins
WO2012112603A1 (en) * 2011-02-14 2012-08-23 University Of Utah Research Foundations Constructs and methods for generating induced pluri potent stem ( ips) cells
WO2016022075A1 (en) * 2014-08-08 2016-02-11 Agency For Science, Technology And Research Mutants of the bacteriophage lambda integrase

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA014757B1 (ru) * 2006-02-28 2011-02-28 Вэксарт, Инк. Химерный аденовирусный вектор, иммуногенная композиция на его основе, способ индукции иммуного ответа с его помощью и выделенная нуклеиновая кислота
US9388425B2 (en) * 2006-10-20 2016-07-12 Trustees Of Boston University Tunable genetic switch for regulating gene expression
EP2097538A4 (en) * 2006-12-07 2011-11-30 Switchgear Genomics TRANSCRIPTION REAGULATION ELEMENTS OF BIOLOGICAL PATHS, TOOLS AND METHODS
DK2477652T3 (en) * 2009-09-16 2015-07-20 Vaxart Inc Immunization strategy for the prevention of infection H1Ni
EP2611915B1 (en) * 2010-09-01 2015-04-15 Cellagenics B.V. Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression
AU2012238601B2 (en) 2011-04-07 2017-01-12 Bayer Cropscience Nv Seed - specific promoter in cotton
US9234197B2 (en) * 2011-10-28 2016-01-12 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona For And On Behalf Of Arizona State University Genetic element that enhances protein translation
DK2711426T3 (en) * 2012-09-24 2015-07-13 Lonza Biologics Plc Expression vectors comprising chimeric cytomegalovirus promoter and enhancer sequences
DK2971005T3 (da) 2013-03-12 2020-08-24 Agc Biologics Inc Forbedret rekombinant proteinekspression ved anvendelse af en hybrid CHEF1-promotor
CN104630267B (zh) * 2014-07-17 2017-10-31 清华大学 利用tale转录抑制子在哺乳动物细胞中模块化构建合成基因线路的试剂盒

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007081336A1 (en) * 2006-01-13 2007-07-19 Five Prime Therapeutics, Inc. Mammalian vectors for high-level expression of recombinant proteins
WO2012112603A1 (en) * 2011-02-14 2012-08-23 University Of Utah Research Foundations Constructs and methods for generating induced pluri potent stem ( ips) cells
WO2016022075A1 (en) * 2014-08-08 2016-02-11 Agency For Science, Technology And Research Mutants of the bacteriophage lambda integrase

Also Published As

Publication number Publication date
WO2017149292A1 (en) 2017-09-08
AU2017225350A1 (en) 2018-09-06
KR102755545B1 (ko) 2025-01-17
JP6960409B2 (ja) 2021-11-05
KR20180132045A (ko) 2018-12-11
GB2553170B (en) 2020-05-06
GB201703224D0 (en) 2017-04-12
US10647999B2 (en) 2020-05-12
SG11201806782QA (en) 2018-09-27
US20190071692A1 (en) 2019-03-07
EP3686282B1 (en) 2024-12-04
GB2578859B (en) 2020-10-28
GB201603577D0 (en) 2016-04-13
EP3423583B1 (en) 2023-05-24
GB2553170A (en) 2018-02-28
GB2578859A (en) 2020-05-27
GB202002193D0 (en) 2020-04-01
EP3423583A1 (en) 2019-01-09
EP3686282A1 (en) 2020-07-29
JP2019506882A (ja) 2019-03-14
CN109072251A (zh) 2018-12-21

Similar Documents

Publication Publication Date Title
AU2017225350B2 (en) Promoter
Schambach et al. Improving transcriptional termination of self-inactivating gamma-retroviral and lentiviral vectors
KR102243243B1 (ko) 신규한 cho 통합 부위 및 이의 용도
JP4489424B2 (ja) 染色体に基くプラットホーム
WO2017212264A1 (en) Method of integrating donor dna into target dna
EP3730616A1 (en) Split single-base gene editing systems and application thereof
JP2023552663A (ja) 安定したレンチウイルスパッケージング細胞株およびその作成方法
WO2013063348A1 (en) Genetic element that enhances protein translation
JP2017200476A (ja) 発現カセット
Wang et al. Shortened nuclear matrix attachment regions are sufficient for replication and maintenance of episomes in mammalian cells
US20250136961A1 (en) Isolated nuclease and use thereof
KR102256749B1 (ko) 고발현 세포주 확립방법
HK40034561A (en) Promoter
CN116555342A (zh) 一种经改造的pT7TS质粒及其应用
HK40034561B (en) Promoter
HK40001212A (en) Promoter
HK40001212B (en) Promoter
WO2005090562A1 (ja) 軽度の低温により遺伝子の発現を促進させる配列
CN113584085B (zh) 一种针对悬浮细胞的慢病毒载体及其应用
Torkzaban et al. Tethered mRNA Amplifier: A Novel Approach to Increase Protein Expression
WO2025064834A1 (en) Isolated betacoronavirus ires sequences
Rouault et al. Promoter complex in the central part of the mouse mammary tumor virus long terminal repeat
CN116218880A (zh) 一种提高病毒滴度的重组载体及其制备方法和应用
Dormiani et al. Rational development of a polycistronic plasmid with a CpG-free bacterial backbone as a potential tool for direct reprogramming

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)