AU2016341938B2 - Methods of preparing a primary cell sample - Google Patents

Methods of preparing a primary cell sample Download PDF

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Publication number
AU2016341938B2
AU2016341938B2 AU2016341938A AU2016341938A AU2016341938B2 AU 2016341938 B2 AU2016341938 B2 AU 2016341938B2 AU 2016341938 A AU2016341938 A AU 2016341938A AU 2016341938 A AU2016341938 A AU 2016341938A AU 2016341938 B2 AU2016341938 B2 AU 2016341938B2
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AU
Australia
Prior art keywords
cell
sample
anoikis
cells
inhibitors
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Ceased
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AU2016341938A
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AU2016341938A1 (en
Inventor
Abhijit Dandapat
Lance Gavin Laing
Ben RICH
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Celcuity Inc
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Celcuity Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2016341938A 2015-10-20 2016-10-20 Methods of preparing a primary cell sample Ceased AU2016341938B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562243765P 2015-10-20 2015-10-20
US62/243,765 2015-10-20
PCT/US2016/057923 WO2017070353A1 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

Publications (2)

Publication Number Publication Date
AU2016341938A1 AU2016341938A1 (en) 2018-04-26
AU2016341938B2 true AU2016341938B2 (en) 2022-12-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016341938A Ceased AU2016341938B2 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

Country Status (9)

Country Link
US (2) US11591573B2 (enExample)
EP (1) EP3365436B1 (enExample)
JP (2) JP7202613B2 (enExample)
KR (1) KR102757122B1 (enExample)
CN (1) CN108138146A (enExample)
AU (1) AU2016341938B2 (enExample)
CA (1) CA2999054A1 (enExample)
DK (1) DK3365436T3 (enExample)
WO (1) WO2017070353A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017070353A1 (en) 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample
CN108300688B (zh) * 2018-02-07 2021-02-02 上海赛立维生物科技有限公司 原代肝细胞分离和培养方法
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736B (zh) * 2019-01-29 2022-03-25 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN109943520B (zh) * 2019-03-08 2023-06-23 中国人民解放军军事科学院军事医学研究院 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
EP4073516A1 (en) 2019-12-09 2022-10-19 Celcuity Inc. Methods of treating cancer patients with ras node or rtk targeted therapeutic agents
CN111733136B (zh) * 2020-06-29 2021-11-30 中山大学孙逸仙纪念医院 一种提高CD90posi细胞分离效率的方法
US20260135422A1 (en) 2021-10-27 2026-05-14 Sumitomo Bakelite Co., Ltd. Stator and structure

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1795897A1 (fr) * 2005-12-12 2007-06-13 Université de Limoges Test prédictif de réponse thérapeutique du cancer du sein
WO2009066258A1 (en) * 2007-11-20 2009-05-28 Hopitaux Universitaires De Geneve Methods for identifying, purifying and enriching immature or stem cancer-initiating cells from tumors and use thereof
WO2013050962A1 (en) * 2011-10-04 2013-04-11 Mitra Biotech Private Limited Ecm composition, tumor microenvironment platform and methods thereof
WO2014132032A1 (en) * 2013-02-27 2014-09-04 Cancer Research Technology Limited Culture of leukemia initiating cells
US20150125894A1 (en) * 2012-06-12 2015-05-07 Celcuity Llc Whole cell assays and methods
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent

Family Cites Families (12)

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SG47996A1 (en) 1993-12-30 1998-04-17 Nitta Gelatin Kk Method for culturing animal cells under embedded conditions
US5858678A (en) 1994-08-02 1999-01-12 St. Louis University Apoptosis-regulating proteins
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
US20090130064A1 (en) 2004-10-27 2009-05-21 Vera Rogiers Differentiation of stem cells and stabilization of phenotypical properties of primary cells
US9096827B2 (en) 2008-09-02 2015-08-04 Pluristem Ltd. Adherent cells from placenta tissue and use thereof in therapy
JP5774496B2 (ja) 2010-01-19 2015-09-09 株式会社ルネッサンス・エナジー・インベストメント 癌組織由来細胞塊または癌細胞凝集塊の培養方法、評価方法および保存方法
EP2566975A4 (en) 2010-05-03 2014-04-02 Microstem Inc SCREENING METHODS, COMPOSITIONS IDENTIFIED BY THIS METHOD, TOOLS USEFUL FOR THEIR IDENTIFICATION AND CELLULAR POPULATIONS THUS PRODUCED
TWI477599B (zh) 2011-01-28 2015-03-21 鴻海精密工業股份有限公司 培育基體
US20140142000A1 (en) * 2012-10-25 2014-05-22 Academia Sinica Microfluidic array platform for simultaneous cell culture under oxygen tensions
CA3106271A1 (en) * 2013-01-25 2014-07-31 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
WO2015156929A1 (en) * 2014-04-07 2015-10-15 The Trustees Of Columbia University In The City Of New York Method for culture of human bladder cell lines and organoids and uses thereof
WO2017070353A1 (en) 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1795897A1 (fr) * 2005-12-12 2007-06-13 Université de Limoges Test prédictif de réponse thérapeutique du cancer du sein
WO2009066258A1 (en) * 2007-11-20 2009-05-28 Hopitaux Universitaires De Geneve Methods for identifying, purifying and enriching immature or stem cancer-initiating cells from tumors and use thereof
WO2013050962A1 (en) * 2011-10-04 2013-04-11 Mitra Biotech Private Limited Ecm composition, tumor microenvironment platform and methods thereof
US20150125894A1 (en) * 2012-06-12 2015-05-07 Celcuity Llc Whole cell assays and methods
WO2014132032A1 (en) * 2013-02-27 2014-09-04 Cancer Research Technology Limited Culture of leukemia initiating cells
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent

Also Published As

Publication number Publication date
EP3365436B1 (en) 2021-09-08
DK3365436T3 (da) 2021-10-11
JP2018531021A (ja) 2018-10-25
KR20180057653A (ko) 2018-05-30
WO2017070353A1 (en) 2017-04-27
US20180230434A1 (en) 2018-08-16
JP7202613B2 (ja) 2023-01-12
EP3365436A1 (en) 2018-08-29
CN108138146A (zh) 2018-06-08
US12215356B2 (en) 2025-02-04
KR102757122B1 (ko) 2025-01-21
CA2999054A1 (en) 2017-04-27
JP2021175415A (ja) 2021-11-04
HK1252635A1 (zh) 2019-05-31
US20230235293A1 (en) 2023-07-27
US11591573B2 (en) 2023-02-28
AU2016341938A1 (en) 2018-04-26

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