JP7202613B2 - 初代細胞試料を調製する方法 - Google Patents

初代細胞試料を調製する方法 Download PDF

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JP7202613B2
JP7202613B2 JP2018520106A JP2018520106A JP7202613B2 JP 7202613 B2 JP7202613 B2 JP 7202613B2 JP 2018520106 A JP2018520106 A JP 2018520106A JP 2018520106 A JP2018520106 A JP 2018520106A JP 7202613 B2 JP7202613 B2 JP 7202613B2
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cell
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anoikis
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JP2018531021A6 (ja
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ランス ギャビン レイン,
ベン リッチ,
アビジット ダンダパット,
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セルキュイティー インコーポレイテッド
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JP2018520106A 2015-10-20 2016-10-20 初代細胞試料を調製する方法 Active JP7202613B2 (ja)

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US201562243765P 2015-10-20 2015-10-20
US62/243,765 2015-10-20
PCT/US2016/057923 WO2017070353A1 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

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CN (1) CN108138146A (enExample)
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CA2999054A1 (en) 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample
CN108300688B (zh) * 2018-02-07 2021-02-02 上海赛立维生物科技有限公司 原代肝细胞分离和培养方法
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736B (zh) * 2019-01-29 2022-03-25 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN109943520B (zh) * 2019-03-08 2023-06-23 中国人民解放军军事科学院军事医学研究院 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
WO2021119056A1 (en) 2019-12-09 2021-06-17 Celcuity Inc. Methods of treating cancer patients with ras node or rtk targeted therapeutic agents
CN111733136B (zh) * 2020-06-29 2021-11-30 中山大学孙逸仙纪念医院 一种提高CD90posi细胞分离效率的方法
JP7452764B2 (ja) 2021-10-27 2024-03-19 住友ベークライト株式会社 ステータ及び構造体

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011090068A1 (ja) 2010-01-19 2011-07-28 株式会社Reiメディカル 癌組織由来細胞塊または癌細胞凝集塊の培養方法、評価方法および保存方法
WO2014117021A2 (en) 2013-01-25 2014-07-31 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
WO2014132032A1 (en) 2013-02-27 2014-09-04 Cancer Research Technology Limited Culture of leukemia initiating cells

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995018216A1 (en) 1993-12-30 1995-07-06 Nitta Gelatin Inc. Process for embedding culture of animal cells
US5858678A (en) 1994-08-02 1999-01-12 St. Louis University Apoptosis-regulating proteins
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
ATE527341T1 (de) 2004-10-27 2011-10-15 Univ Bruxelles Hepatische differenzierung von stammzellen
FR2894594B1 (fr) * 2005-12-12 2008-02-08 Univ Limoges Test predictif de reponse therapeutique du cancer du sein
ES2535929T3 (es) * 2007-11-20 2015-05-19 Hopitaux Universitaires De Genève Métodos para identificar, purificar y enriquecer células madre o inmaduras de inicio de cáncer a partir de tumores y uso de las mismas
US9096827B2 (en) 2008-09-02 2015-08-04 Pluristem Ltd. Adherent cells from placenta tissue and use thereof in therapy
CN103025887A (zh) 2010-05-03 2013-04-03 麦克罗斯特姆公司 筛选方法、由其确定的组合物、可用于其确定的工具和由其生产的细胞群
TWI477599B (zh) 2011-01-28 2015-03-21 Hon Hai Prec Ind Co Ltd 培育基體
PT2764099T (pt) * 2011-10-04 2018-02-28 Mitra Rxdx India Private Ltd Composição de ecm, plataforma de microambiente tumoral e seus métodos
US20130330761A1 (en) 2012-06-12 2013-12-12 Celcuity, LLC Whole cell assays and methods
US20140142000A1 (en) 2012-10-25 2014-05-22 Academia Sinica Microfluidic array platform for simultaneous cell culture under oxygen tensions
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent
WO2015156929A1 (en) * 2014-04-07 2015-10-15 The Trustees Of Columbia University In The City Of New York Method for culture of human bladder cell lines and organoids and uses thereof
CA2999054A1 (en) * 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011090068A1 (ja) 2010-01-19 2011-07-28 株式会社Reiメディカル 癌組織由来細胞塊または癌細胞凝集塊の培養方法、評価方法および保存方法
WO2014117021A2 (en) 2013-01-25 2014-07-31 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
WO2014132032A1 (en) 2013-02-27 2014-09-04 Cancer Research Technology Limited Culture of leukemia initiating cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
12634 - Advanced D-MEM/F-12,Thermo Fischer Scientific,[令和2年7月10日検索], インターネット<URL:https://www.thermofisher.com/jp/ja/home/technical-resources/media-formulation.227.html>
StemPro hESC SFM, [online],Thermo Fischer Scientific Inc.,[令和2年7月10日検索], インターネット<URL:http://tools.thermofisher.com/content/sfs/manuals/stempro_hESC_SFM_man.pdf>

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WO2017070353A1 (en) 2017-04-27
US20230235293A1 (en) 2023-07-27
CN108138146A (zh) 2018-06-08
JP2021175415A (ja) 2021-11-04
CA2999054A1 (en) 2017-04-27
AU2016341938A1 (en) 2018-04-26
US20180230434A1 (en) 2018-08-16
KR20180057653A (ko) 2018-05-30
DK3365436T3 (da) 2021-10-11
EP3365436A1 (en) 2018-08-29
KR102757122B1 (ko) 2025-01-21
US11591573B2 (en) 2023-02-28
US12215356B2 (en) 2025-02-04
JP2018531021A (ja) 2018-10-25
AU2016341938B2 (en) 2022-12-01
HK1252635A1 (zh) 2019-05-31
EP3365436B1 (en) 2021-09-08

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