HK1252635A1 - 製備原代細胞樣品的方法 - Google Patents

製備原代細胞樣品的方法

Info

Publication number
HK1252635A1
HK1252635A1 HK18111938.2A HK18111938A HK1252635A1 HK 1252635 A1 HK1252635 A1 HK 1252635A1 HK 18111938 A HK18111938 A HK 18111938A HK 1252635 A1 HK1252635 A1 HK 1252635A1
Authority
HK
Hong Kong
Prior art keywords
preparing
methods
primary cell
cell sample
sample
Prior art date
Application number
HK18111938.2A
Other languages
English (en)
Inventor
Lance Gavin Laing
Ben Rich
Abhijit Dandapat
Original Assignee
Celcuity Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celcuity Inc filed Critical Celcuity Inc
Publication of HK1252635A1 publication Critical patent/HK1252635A1/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
HK18111938.2A 2015-10-20 2018-09-17 製備原代細胞樣品的方法 HK1252635A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201562243765P 2015-10-20 2015-10-20

Publications (1)

Publication Number Publication Date
HK1252635A1 true HK1252635A1 (zh) 2019-05-31

Family

ID=57233884

Family Applications (1)

Application Number Title Priority Date Filing Date
HK18111938.2A HK1252635A1 (zh) 2015-10-20 2018-09-17 製備原代細胞樣品的方法

Country Status (10)

Country Link
US (2) US11591573B2 (zh)
EP (1) EP3365436B1 (zh)
JP (2) JP7202613B2 (zh)
KR (1) KR20180057653A (zh)
CN (1) CN108138146A (zh)
AU (1) AU2016341938B2 (zh)
CA (1) CA2999054A1 (zh)
DK (1) DK3365436T3 (zh)
HK (1) HK1252635A1 (zh)
WO (1) WO2017070353A1 (zh)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017070353A1 (en) * 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample
CN108300688B (zh) * 2018-02-07 2021-02-02 上海赛立维生物科技有限公司 原代肝细胞分离和培养方法
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736B (zh) * 2019-01-29 2022-03-25 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN115927162A (zh) * 2019-03-08 2023-04-07 中国人民解放军军事科学院军事医学研究院 汗腺细胞的培养获得汗腺类器官的方法及其应用
JP2023505526A (ja) 2019-12-09 2023-02-09 セルキュイティー インコーポレイテッド Ｒａｓノード標的化治療剤またはｒｔｋ標的化治療剤を用いてがん患者を処置する方法
CN111733136B (zh) * 2020-06-29 2021-11-30 中山大学孙逸仙纪念医院 一种提高CD90posi细胞分离效率的方法
JP7452764B2 (ja) 2021-10-27 2024-03-19 住友ベークライト株式会社 ステータ及び構造体

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG47996A1 (en) 1993-12-30 1998-04-17 Nitta Gelatin Kk Method for culturing animal cells under embedded conditions
US5858678A (en) 1994-08-02 1999-01-12 St. Louis University Apoptosis-regulating proteins
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
EP1824965B1 (en) 2004-10-27 2011-10-05 Vrije Universiteit Brussel Hepatic differentiation of stem cells
FR2894594B1 (fr) * 2005-12-12 2008-02-08 Univ Limoges Test predictif de reponse therapeutique du cancer du sein
CA2706293A1 (en) * 2007-11-20 2009-05-28 Hopitaux Universitaires De Geneve Methods for identifying, purifying and enriching immature or stem cancer-initiating cells from tumors and use thereof
WO2010026574A2 (en) 2008-09-02 2010-03-11 Pluristem Ltd. Adherent cells from placenta tissue and use thereof in therapy
WO2011090068A1 (ja) * 2010-01-19 2011-07-28 株式会社Ｒｅｉメディカル 癌組織由来細胞塊または癌細胞凝集塊の培養方法、評価方法および保存方法
CN103025887A (zh) 2010-05-03 2013-04-03 麦克罗斯特姆公司 筛选方法、由其确定的组合物、可用于其确定的工具和由其生产的细胞群
TWI477599B (zh) 2011-01-28 2015-03-21 Hon Hai Prec Ind Co Ltd 培育基體
AU2012320088C1 (en) * 2011-10-04 2016-03-17 Mitra Rxdx India Private Limited ECM composition, tumor microenvironment platform and methods thereof
US20130330761A1 (en) 2012-06-12 2013-12-12 Celcuity, LLC Whole cell assays and methods
US20140142000A1 (en) * 2012-10-25 2014-05-22 Academia Sinica Microfluidic array platform for simultaneous cell culture under oxygen tensions
GB2516196B (en) * 2013-01-25 2015-09-09 Xcell Biosciences Inc Methods, compositions, kits, and systems for selective enrichment of target cells
GB201303491D0 (en) * 2013-02-27 2013-04-10 Cancer Rec Tech Ltd Culture of leukemia initiating cells
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent
WO2015156929A1 (en) * 2014-04-07 2015-10-15 The Trustees Of Columbia University In The City Of New York Method for culture of human bladder cell lines and organoids and uses thereof
WO2017070353A1 (en) * 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample

Also Published As

Publication number Publication date
US20230235293A1 (en) 2023-07-27
JP2018531021A (ja) 2018-10-25
KR20180057653A (ko) 2018-05-30
EP3365436B1 (en) 2021-09-08
JP2021175415A (ja) 2021-11-04
CN108138146A (zh) 2018-06-08
DK3365436T3 (da) 2021-10-11
US20180230434A1 (en) 2018-08-16
EP3365436A1 (en) 2018-08-29
US11591573B2 (en) 2023-02-28
WO2017070353A1 (en) 2017-04-27
JP7202613B2 (ja) 2023-01-12
CA2999054A1 (en) 2017-04-27
AU2016341938A1 (en) 2018-04-26
AU2016341938B2 (en) 2022-12-01

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