CN108138146A - 制备原代细胞样品的方法 - Google Patents

制备原代细胞样品的方法 Download PDF

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Publication number
CN108138146A
CN108138146A CN201680061008.6A CN201680061008A CN108138146A CN 108138146 A CN108138146 A CN 108138146A CN 201680061008 A CN201680061008 A CN 201680061008A CN 108138146 A CN108138146 A CN 108138146A
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cell
anoikis
inhibitor
sample
cells
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CN201680061008.6A
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Chinese (zh)
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L·G·莱恩
B·里驰
A·丹大帕特
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Celcuity Inc
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Celcuity Inc
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
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    • C12N2533/52—Fibronectin; Laminin
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • Oncology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
CN201680061008.6A 2015-10-20 2016-10-20 制备原代细胞样品的方法 Pending CN108138146A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562243765P 2015-10-20 2015-10-20
US62/243,765 2015-10-20
PCT/US2016/057923 WO2017070353A1 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

Publications (1)

Publication Number Publication Date
CN108138146A true CN108138146A (zh) 2018-06-08

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CN201680061008.6A Pending CN108138146A (zh) 2015-10-20 2016-10-20 制备原代细胞样品的方法

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US (2) US11591573B2 (enExample)
EP (1) EP3365436B1 (enExample)
JP (2) JP7202613B2 (enExample)
KR (1) KR102757122B1 (enExample)
CN (1) CN108138146A (enExample)
AU (1) AU2016341938B2 (enExample)
CA (1) CA2999054A1 (enExample)
DK (1) DK3365436T3 (enExample)
WO (1) WO2017070353A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736A (zh) * 2019-01-29 2019-05-21 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN109943520A (zh) * 2019-03-08 2019-06-28 北京达博威迎医药技术有限公司 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2999054A1 (en) 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample
CN108300688B (zh) * 2018-02-07 2021-02-02 上海赛立维生物科技有限公司 原代肝细胞分离和培养方法
WO2021119056A1 (en) 2019-12-09 2021-06-17 Celcuity Inc. Methods of treating cancer patients with ras node or rtk targeted therapeutic agents
CN111733136B (zh) * 2020-06-29 2021-11-30 中山大学孙逸仙纪念医院 一种提高CD90posi细胞分离效率的方法
JP7452764B2 (ja) 2021-10-27 2024-03-19 住友ベークライト株式会社 ステータ及び構造体

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EP1795897A1 (fr) * 2005-12-12 2007-06-13 Université de Limoges Test prédictif de réponse thérapeutique du cancer du sein
TW201439537A (zh) * 2012-10-25 2014-10-16 Academia Sinica 用於氧氣濃度控制下同步細胞培養的微流體陣列平台
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent

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CN103025887A (zh) 2010-05-03 2013-04-03 麦克罗斯特姆公司 筛选方法、由其确定的组合物、可用于其确定的工具和由其生产的细胞群
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736A (zh) * 2019-01-29 2019-05-21 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN109943520A (zh) * 2019-03-08 2019-06-28 北京达博威迎医药技术有限公司 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
CN109943520B (zh) * 2019-03-08 2023-06-23 中国人民解放军军事科学院军事医学研究院 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用

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Publication number Publication date
WO2017070353A1 (en) 2017-04-27
US20230235293A1 (en) 2023-07-27
JP7202613B2 (ja) 2023-01-12
JP2021175415A (ja) 2021-11-04
CA2999054A1 (en) 2017-04-27
AU2016341938A1 (en) 2018-04-26
US20180230434A1 (en) 2018-08-16
KR20180057653A (ko) 2018-05-30
DK3365436T3 (da) 2021-10-11
EP3365436A1 (en) 2018-08-29
KR102757122B1 (ko) 2025-01-21
US11591573B2 (en) 2023-02-28
US12215356B2 (en) 2025-02-04
JP2018531021A (ja) 2018-10-25
AU2016341938B2 (en) 2022-12-01
HK1252635A1 (zh) 2019-05-31
EP3365436B1 (en) 2021-09-08

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