AU2016335698B2 - Multiplexed genome editing - Google Patents

Multiplexed genome editing Download PDF

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Publication number
AU2016335698B2
AU2016335698B2 AU2016335698A AU2016335698A AU2016335698B2 AU 2016335698 B2 AU2016335698 B2 AU 2016335698B2 AU 2016335698 A AU2016335698 A AU 2016335698A AU 2016335698 A AU2016335698 A AU 2016335698A AU 2016335698 B2 AU2016335698 B2 AU 2016335698B2
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cell
perv
porcine
genetically engineered
sequence
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AU2016335698A1 (en
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George M. Church
Marc Guell
Luhan Yang
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Harvard University
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Harvard University
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    • A—HUMAN NECESSITIES
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
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    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
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    • A01K67/027—New or modified breeds of vertebrates
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    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
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    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1132—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against retroviridae, e.g. HIV
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • A01K2207/00—Modified animals
    • A01K2207/05—Animals modified by non-integrating nucleic acids, e.g. antisense, RNAi, morpholino, episomal vector, for non-therapeutic purpose
    • A—HUMAN NECESSITIES
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2740/00—Reverse transcribing RNA viruses
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AU2016335698A 2015-10-08 2016-10-07 Multiplexed genome editing Active AU2016335698B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2023201178A AU2023201178B2 (en) 2015-10-08 2023-02-28 Multiplexed genome editing

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Application Number Priority Date Filing Date Title
US201562239239P 2015-10-08 2015-10-08
US62/239,239 2015-10-08
PCT/US2016/055916 WO2017062723A1 (en) 2015-10-08 2016-10-07 Multiplexed genome editing

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AU2016335698A1 AU2016335698A1 (en) 2018-05-10
AU2016335698B2 true AU2016335698B2 (en) 2022-12-01

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US (6) US10959413B2 (cg-RX-API-DMAC7.html)
EP (2) EP4491732A3 (cg-RX-API-DMAC7.html)
JP (4) JP7059468B2 (cg-RX-API-DMAC7.html)
KR (2) KR102880582B1 (cg-RX-API-DMAC7.html)
CN (2) CN116064664A (cg-RX-API-DMAC7.html)
AU (2) AU2016335698B2 (cg-RX-API-DMAC7.html)
CA (1) CA3001314A1 (cg-RX-API-DMAC7.html)
CL (1) CL2018000902A1 (cg-RX-API-DMAC7.html)
CO (1) CO2018004763A2 (cg-RX-API-DMAC7.html)
IL (2) IL297017A (cg-RX-API-DMAC7.html)
MX (2) MX2018004263A (cg-RX-API-DMAC7.html)
NZ (1) NZ741778A (cg-RX-API-DMAC7.html)
SA (2) SA518391294B1 (cg-RX-API-DMAC7.html)
WO (1) WO2017062723A1 (cg-RX-API-DMAC7.html)
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